成果/Result
- Refolding, purification and characterization of an organic solvent-tolerant lipase from Serratia marcescens ECU1010被引量：37收藏
- 作者：Li, Suxia Pang, Huaiyu Lin, Kang Xu, Jianhe Zhao, Jian Fan, Liqiang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Cloning - Chromatography - Recombinant protEIns - Purification - Organic solvents
- Significantly improved expression and biochemical properties of recombinant serratia marcescens lipase as robust biocatalyst for kinetic resolution of chiral ester被引量：33收藏
- 作者：Wang, Yi Zhao, Jian Xu, Jian-He Fan, Li-Qiang Li, Su-Xia Zhao, Li-Li Mao, Xiao-Bo
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Biochemistry and Biotechnology 2010
- 关键词：Esters - Gene expression - Recombinant protEIns - Escherichia coli - Fatty acids - Affinity chromatography - Lipases - Substrates - Cloning - Metal ions - Metals - Nonionic surfactants - Purification
- Expression and Characterization of a Novel Lipase from Aspergillus fumigatus with High Specific Activity被引量：31收藏
- 作者：Shangguan, Jiao-Jiao Liu, Yu-Qiang Wang, Fu-Jun Zhao, Jian Fan, Li-Qiang Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Aspergillus fumigatus Recombinant lipase Expression Characterization Active site
- caspase-mediated LPS sensing and pyroptosis signaling in Hydra被引量：31收藏
- 作者：Chen, Shouwen Li, Shuxin Chen, Hao Gong, Yuxin Yang, Dahai Zhang, Yuanxing Liu, Qin
- 机构：East China Univ Sci & Technol;Qingdao Natl Lab Marine Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai
- 来源：SCIENCE ADVANCES 2023
- 关键词：Network security
- Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol被引量：29收藏
- 作者：Zhu, Qing-Qing He, Wan-Hong Kong, Xu-Dong Fan, Li-Qiang Zhao, Jian Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：RT-PCR Vigna radiata epoxide hydrolase p-Nitrostyrene oxide Enantioconvergency Characterization
- Effect of caCl2 as activity stabilizer on purification of heparinase I from Flavobacterium heparinum被引量：29收藏
- 作者：Ma, Xiaolai Wang, Zunsheng Li, Suxia Shen, Qiong Yuan, Qinsheng
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biology
- 来源：Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 2006
- 关键词：Bacteria - Cell culture - Chromatography - pH effects - Precipitation (chemical) - Purification
- Production, Characterization, and Application of an Organic Solvent-Tolerant Lipase Present in Active Inclusion Bodies被引量：16收藏
- 作者：Li, Suxia Lin, Kang Pang, Huaiyu Wu, Yixin Xu, Jianhe
- 机构：E China Univ Sci & Technol;Hamamatsu Univ Sch Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2013
- 关键词：Lipase Active inclusion body Organic solvent tolerance (+/-)-MPGM Serratia marcescens ECU1010
- 钝顶螺旋藻Fe-SOD的纯化及性质被引量：12收藏
- 作者：夏文超 李素霞 范立强 袁勤生
- 机构：华东理工大学生物反应器工程国家重点实验室生物工程学院
- 来源：《华东理工大学学报（自然科学版）》 2003
- 关键词：Fe-SOD 钝顶螺旋藻 铁型超氧化物歧化酶 纯化 平均比活 稳定性 金属酶
- 摘要：从螺旋藻中分离纯化的Fe-SOD平均比活为4576U/mg,总活力回收率50%,纯度达电泳纯。SDS-PAGE测定纯化的Fe-SOD亚基相对分子质量为21ku,每分子亚基含0.7个Fe原子;在Tris-盐酸缓冲液中的最适...
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- 重组羧肽酶B在胰岛素原C肽制备工艺中的应用被引量：9收藏
- 作者：李素霞 田丽萍 张晓彦 夏文超 龚毅 袁勤生
- 机构：华东理工大学生物反应器工程国家重点实验室;中国科学院上海生命科学研究院生物工程中心
- 来源：《华东理工大学学报（自然科学版）》 2004
- 关键词：羧肽酶原B 羧肽酶B 融合表达 分离纯化 胰岛素原C肽
- 摘要：用RT-PCR方法克隆了大鼠羧肽酶原B的cDNA编码序列,将其重组到原核表达质粒pT7-473中,并在大肠杆菌中以包涵体方式获得高表达。SDS-PAGE电泳及灰度扫描显示表达量达菌体总蛋白的35%,表达产物融合了表达质粒...
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- Preparation and Irreversible Inhibition Mechanism Insight into a Recombinant Kunitz Trypsin Inhibitor from Glycine max L. Seeds被引量：9收藏
- 作者：Xu, Yanji Zhang, Panpan Liu, Xiao Wang, Zhike Li, Suxia
- 机构：East China Univ Sci & Technol;Shanghai Yaxin Biotechnol Ltd Co
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2020
- 关键词：Soybean Kunitz trypsin inhibitor ProtEIn refolding Biochemical property Inhibition kinetic assay Molecular modeling
- Essential Role of Gly33 in a Novel Organic Solvent-Tolerant Lipase from Serratia marcescens ECU1010 as Determined by Site-Directed Mutagenesis被引量：5收藏
- 作者：Li, Su-Xia Ma, Qiang Lin, Kang Wu, Jiao-Jiao Wu, Yi-Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol;Hamamatsu Univ Sch Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2014
- 关键词：Lipase Site-directed mutagenesis Organic solvent stability Molecular modeling Serratia marcescens ECU1010
- Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B被引量：2收藏
- 作者：Li, Suxia Zhang, Luosheng Wu, Qian Xin, Aijie Zhao, Jian Fan, Liqiang
- 机构：E China Univ Sci & Technol
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2011
- 关键词：Procarboxypeptidase B Mutation CystEIne Refolding efficiency Property
- Purification and partial characterization of recombinant Cu, Zn containing superoxide dismutase of Cordyceps militaris in E. coli被引量：0收藏
- 作者：Wang, Zunsheng He, Zhuojing Shen, Qiong Gu, Yuxiang Li, Suxia Yuan, Qinsheng
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biology
- 来源：Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 2005
- 关键词：Amino acids - Characterization - Copper oxides - DNA - Escherichia coli - pH - Purification - Zinc oxide
