成果/Result
- The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction被引量：194收藏
- 作者：Liu, Guo-Song Li, Tian Zhou, Wei Jiang, Min Tao, Xin-Yi Liu, Min Zhao, Ming Ren, Yu-Hong Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2020
- 关键词：Synthetic biology Saccharomyces cerevisiae Peroxisome Storage depot Subcellular factory Terpene
- Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction被引量：115收藏
- 作者：Zhu, Zhan-Tao Du, Meng-Meng Gao, Bei Tao, Xin-Yi Zhao, Ming Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2021
- 关键词：Saccharomyces cerevisiae Mitochondrial engineering Metabolic burden Squalene Dual engineering
- The Important Role of Halogen Bond in Substrate Selectivity of Enzymatic Catalysis被引量：78收藏
- 作者：Jiang, Shuiqin Zhang, Lujia Cui, Dongbin Yao, Zhiqiang Gao, Bei Lin, Jinping Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：SCIENTIFIC REPORTS 2016
- Metabolic Engineering of Saccharomyces cerevisiae To Overproduce Squalene被引量：71收藏
- 作者：Li, Tian Liu, Guo-Song Zhou, Wei Jiang, Min Ren, Yu-Hong Tao, Xin-Yi Liu, Min Zhao, Ming Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Saccharomyces cerevisiae metabolic engineering squalene overproduction ethanol carbon source utilization
- High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae被引量：57收藏
- 作者：Liu, Min Lin, Yang-Chen Guo, Jiao-Jiao Du, Meng-Meng Tao, Xinyi Gao, Bei Zhao, Ming Ma, Yushu Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2021
- 关键词：patchoulol physiological status metabolic engineering Saccharomyces cerevisiae sesquiterpene cell factories
- A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters被引量：55收藏
- 作者：Gao, Wenyuan Wu, Kai Chen, Lifeng Fan, Haiyang Zhao, Zhiqiang Gao, Bei Wang, Hualei Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2016
- 关键词：Metagenomic library Functional screening Esterase Transesterification Short-chain flavor esters High substrate loading
- Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp for biodiesel production被引量：45收藏
- 作者：Gao, Bei Su, Erzheng Lin, Jinping Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2009
- 关键词：LipK107 E. coli whole-cell catalyst Biodiesel Low-temperature property
- Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus for baking industry and its magnetic immobilization被引量：42收藏
- 作者：He, Lei Mao, Youzhi Zhang, Lujia Wang, Hualei Alias, Siti Aisyah Gao, Bei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Univ Malaya
- 来源：BMC BIOTECHNOLOGY 2017
- 关键词：alpha-Amylase Antarctic fungus Biochemical properties Bread quality Immobilization
- Engineering Saccharomyces cerevisiae for Hyperproduction of β-Amyrin by Mitigating the Inhibition Effect of Squalene on β-Amyrin Synthase被引量：35收藏
- 作者：Du, Meng-Meng Zhu, Zhan-Tao Zhang, Ge-Ge Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Liu, Min Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：beta-amyrin squalene inhibition peroxisome compartmentalization competition pathway Saccharomyces cerevisiae
- Investigation on the characteristics and mechanisms of ACE inhibitory peptides by a thorough analysis of all 8000 tripeptides via binding free energy calculation被引量：34收藏
- 作者：Chen, Ruiyao Miao, Yulu Hao, Xuan Gao, Bei Ma, Mingzhe Zhang, John Z. H. Wang, Rui Li, Sha He, Xiao Zhang, Lujia
- 机构：East China Univ Sci & Technol;East China Normal Univ;NYU Shanghai;NYU;Nanjing Tech Univ
- 来源：FOOD SCIENCE & NUTRITION 2021
- 关键词：ACE inhibitor in silico experiment inhibitory mechanism tripeptides
- High-yield porphyrin production through metabolic engineering and biocatalysis被引量：34收藏
- 作者：Chen, Haihong Wang, Yaohong Wang, Weishan Cao, Ting Zhang, Lu Wang, Zhengduo Chi, Xuran Shi, Tong Wang, Huangwei He, Xinwei Liang, Mindong Yang, Mengxue Jiang, Wenyi Lv, Dongyuan Yu, Jiaming Zhu, Guoliang Xie, Yongtao Gao, Bei Wang, Xinye Liu, Xueting Li, Youyuan Ouyang, Limin Zhang, Jingyu Liu, Huimin Li, Zilong Tong, Yaojun Xia, Xuekui Tan, Gao-Yi Zhang, Lixin
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ;Qilu Univ Technol
- 来源：NATURE BIOTECHNOLOGY 2025
- 关键词：Catalysis - Diagnosis - Fermentation - Medical applications - Metabolic engineering
- A method to rationally increase protein stability based on the charge-charge interaction, with application to lipase LipK107被引量：33收藏
- 作者：Zhang, Lujia Tang, Xiaomang Cui, Dongbing Yao, Zhiqiang Gao, Bei Jiang, Shuiqin Yin, Bo Yuan, Y. Adam Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Nanjing Univ Technol;Natl Univ Singapore;Natl Univ Singapore
- 来源：PROTEIN SCIENCE 2014
- 关键词：designed protein stabilization electrostatic stabilization lipase stabilization surface charge-charge interaction Lipk107
- Umami polypeptide detection system targeting the human T1R1 receptor and its taste-presenting mechanism被引量：32收藏
- 作者：Zhang, Chuanxi Miao, Yulu Feng, Yinghui Wang, Jiawei Tian, Zhuoli Dong, Juan Gao, Bei Zhang, Lujia
- 机构：East China Normal Univ;East China Univ Sci & Technol;NYU Shanghai;Shanghai Jiao Tong Univ;Univ Shanghai Sci & Tecchnol
- 来源：BIOMATERIALS 2022
- 关键词：Umami petides hT1R1 receptor Interaction Detection system
- Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface被引量：32收藏
- 作者：Jiang, Zhengbing Gao, Bei Ren, Ren Tao, Xingyi Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Hubei Univ
- 来源：BMC BIOTECHNOLOGY 2008
- Switching a nitrilase from Syechocystis sp PCC6803 to a nitrile hydratase by rationally regulating reaction pathways被引量：27收藏
- 作者：Jiang, Shuiqin Zhang, Lujia Yao, Zhiqiang Gao, Bei Wang, Hualei Mao, Xiangzhao Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Ocean Univ China
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2017
- 关键词：Amides - Biochemical engineering - Enzymes
- Novel Application of Magnetic Protein: Convenient One-Step Purification and Immobilization of Proteins被引量：27收藏
- 作者：Jiang, Min Zhang, Lujia Wang, Fengqing Zhang, Jie Liu, Guosong Gao, Bei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;East China Normal Univ;NYU Shanghai;East China Normal Univ
- 来源：SCIENTIFIC REPORTS 2017
- A computational strategy for altering an enzyme in its cofactor preference to NAD(H) and/or NADP(H)被引量：23收藏
- 作者：Cui, Dongbing Zhang, Lujia Jiang, Shuiqin Yao, Zhiqiang Gao, Bei Lin, Jinping Yuan, Y. Adam Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Natl Univ Singapore
- 来源：FEBS JOURNAL 2015
- 关键词：altered coenzyme specificity molecular dynamics simulation rational computational design site-directed mutagenesis structure stability prediction
- Improving the catalytic activity of lipase LipK107 from Proteus sp. by site-directed mutagenesis in the lid domain based on computer simulation被引量：21收藏
- 作者：Gao, Bei Xu, Tao Lin, Jinping Zhang, Lujia Su, Erzheng Jiang, Zhengbing Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Hubei Univ
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：LipK107 1-Phenylethanol Lid Site-directed mutagenesis Computer simulation
- Boosting the epoxidation of squalene to produce triterpenoids in Saccharomyces cerevisiae被引量：20收藏
- 作者：Du, Meng-Meng Zhang, Ge-Ge Zhu, Zhan-Tao Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 2023
- 关键词：2,3-Oxidosqualene 2,3:22,23-Dioxidosqualene Triterpenoid ERG7 Two-stage fermentation
- De novo design of a transcription factor for a progesterone biosensor被引量：20收藏
- 作者：Liu, Kun Zhang, Yunsen Liu, Ke Zhao, Yunqiu Gao, Bei Tao, Xinyi Zhao, Ming Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chengdu Univ Tradit Chinese Med
- 来源：BIOSENSORS & BIOELECTRONICS 2022
- 关键词：Artificial transcription factor Molecular dynamics simulation Progesterone biosensor Whole-cell Diagnosis
- Multidimensional engineering of Saccharomyces cerevisiae for the efficient production of heme by exploring the cytotoxicity and tolerance of heme被引量：20收藏
- 作者：Guo, Qidi Li, Jiacun Wang, Ming-Rui Zhao, Ming Zhang, Gege Tang, Shuyan Xiong, Liang-Bin Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2024
- 关键词：Heme Saccharomyces cerevisiae Metabolic engineering Heme cytotoxicity Laboratory evolution
- Construction of a whole-cell catalyst displaying a fungal lipase for effective treatment of oily wastewaters被引量：19收藏
- 作者：Song, Huiting Zhou, Lichao Zhang, Lujia Gao, Bei Wei, Dongzhi Shen, Yaling Wang, Rui Madzak, Catherine Jiang, Zhengbing
- 机构：Hubei Univ;E China Univ Sci & Technol;Hubei Univ;AgroParistech
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：Activated sludge Bioremediation Cell surface display Lipase Oily wastewater Yarrowia lipolytica
- A Self-Sustained System Spanning the Primary and Secondary Metabolism Stages to Boost the Productivity of Streptomyces被引量：19收藏
- 作者：Zhao, Ming Wang, Mingrui Wang, Shuiling Xiong, Liangbin Gao, Bei Liu, Min Tao, Xinyi Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：Streptomyces sigma(hrdB) PkasO* mutant metabolic regulation
- Development of a novel Aspergillus uracil deficient expression system and its application in expressing a cold-adapted α-amylase gene from Antarctic fungi Geomyces pannorum被引量：18收藏
- 作者：Mao, Youzhi Yin, Yanchen Zhang, Lujia Alias, Siti Aisyah Gao, Bei Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Univ Malaya
- 来源：PROCESS BIOCHEMISTRY 2015
- 关键词：Cold-adapted alpha-amylase pyrF Aspergillus oryzae Geomyces pannorum Heterologous expression
- Transformation of phytosterols into pregnatetraenedione by a combined microbial and chemical process被引量：18收藏
- 作者：Liu, Yong-Jun Ji, Wei-ting Song, Lu Tao, Xin-Yi Zhao, Ming Gao, Bei Meng, Hao Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Hunan Norchem Pharmaceut Co Ltd
- 来源：GREEN CHEMISTRY 2022
- 关键词：Alcohols - Carboxylation - Drug products - Metabolism - Metabolites - Polyols
- Engineering Saccharomyces cerevisiae for Hyperproduction of β-Amyrin by Mitigating the Inhibition Effect of Squalene on β-Amyrin Synthase被引量：17收藏
- 作者：Du, Meng-Meng Zhu, Zhan-Tao Zhang, Ge-Ge Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Liu, Min Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：beta-amyrin squalene inhibition peroxisome compartmentalization competition pathway Saccharomyces cerevisiae
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- Engineering Komagataella phaffii to produce lycopene sustainably from glucose or methanol被引量：15收藏
- 作者：Zhou, Wei Ling, Rui-Jing Yang, Yi-Chen Hou, Shu-Ting Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2025
- 关键词：Komagataella phaffii Metabolic engineering Dynamic regulation Transcriptome analysis Methanol Lycopene
- In vivo functional expression of a screened P. aeruginosa chaperone-dependent lipase in E-coli被引量：14收藏
- 作者：Wu, Xiangping You, Pengyong Su, Erzheng Xu, Jingjing Gao, Bei Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BMC BIOTECHNOLOGY 2012
- 关键词：Pseudomonas aeruginosa Lipase Chaperone Dual expression plasmid Esterification
- Light-driven progesterone production by InP-(M. neoaurum) biohybrid system被引量：14收藏
- 作者：Liu, Kun Wang, Feng-Qing Liu, Ke Zhao, Yunqiu Gao, Bei Tao, Xinyi Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Synthetic biology Progesterone P450 Electron transfer InP nanoparticles NADPH/NADP(+)
