成果/Result
- Structural insights into enzymatic activity and substrate specificity determination by a single amino acid in nitrilase from Syechocystis sp PCC6803被引量：60收藏
- 作者：Zhang, Lujia Yin, Bo Wang, Chao Jiang, Shuiqin Wang, Hualei Yuan, Y. Adam Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Natl Univ Singapore;Natl Univ Singapore;Natl Univ Singapore
- 来源：JOURNAL OF STRUCTURAL BIOLOGY 2014
- 关键词：Crystal structure Nitrilase Syechocystis sp PCC6803 Substrate specificity determination
- A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters被引量：55收藏
- 作者：Gao, Wenyuan Wu, Kai Chen, Lifeng Fan, Haiyang Zhao, Zhiqiang Gao, Bei Wang, Hualei Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2016
- 关键词：Metagenomic library Functional screening Esterase Transesterification Short-chain flavor esters High substrate loading
- A turn-on near-infrared fluorescence probe with aggregation-induced emission based on dibenzo [a,c]phenazine for detection of superoxide anions and its application in cell imaging被引量：46收藏
- 作者：Yang, Ji Liu, Xianglong Wang, Honglei Tan, Haoqi Xie, Xiaoxu Zhang, Xiao Liu, Chenchen Qu, Xue Hua, Jianli
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ANALYST 2018
- Discovery and characterization of a highly efficient enantioselective mandelonitrile hydrolase from Burkholderia cenocepacia J2315 by phylogeny-based enzymatic substrate specificity prediction被引量：44收藏
- 作者：Wang, Hualei Sun, Huihui Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BMC BIOTECHNOLOGY 2013
- 关键词：(R)-(-)-mandelic acid Nitrilase Burkholderia cenocepacia J2315 Substrate specificity prediction Enantioselective hydrolysis
- Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus for baking industry and its magnetic immobilization被引量：42收藏
- 作者：He, Lei Mao, Youzhi Zhang, Lujia Wang, Hualei Alias, Siti Aisyah Gao, Bei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Univ Malaya
- 来源：BMC BIOTECHNOLOGY 2017
- 关键词：alpha-Amylase Antarctic fungus Biochemical properties Bread quality Immobilization
- Process Development for the Production of (R)-(-)-Mandelic Acid by Recombinant Escherichia coli Cells Harboring Nitrilase from Burkholderia cenocepacia J2315被引量：36收藏
- 作者：Wang, Hualei Fan, Haiyang Sun, Huihui Zhao, Li Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2015
- 关键词：Cost effectiveness - Organic acids - Hydrolysis
- Magnetic Catechol-Chitosan with Bioinspired Adhesive Surface: Preparation and Immobilization of ω-Transaminase被引量：32收藏
- 作者：Ni, Kefeng Zhou, Xu Zhao, Li Wang, Hualei Ren, Yuhong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：PLOS ONE 2012
- Efficient production of (R)-(-)-mandelic acid in biphasic system by immobilized recombinant E. coli被引量：32收藏
- 作者：Ni, Kefeng Wang, Hualei Zhao, Li Zhang, Minjie Zhang, Siyuan Ren, Yuhong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Biotechnology 2013
- 关键词：Hydrolysis - Molecular biology - Solutions - Organic acids - Cell culture - Iron oxides - Escherichia coli - Cells
- Combinatorial Evolution of Site-Guided Function Switch of Nitrilase to Nitrile Hydratase: Alleviating the Trade-Off between Hydration Activity and Specific Activity被引量：31收藏
- 作者：Sun, Yangyang Tang, Zhuzhu Pan, Tingze Zhang, Ke Wang, Liuzhu Zhai, Xingye Jia, Yiran Yuan, Tianqi Qian, Yujia Wang, Hualei Wei, Dongzhi Yang, Shengli
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：SSRN 2023
- 关键词：Economic and social effects - Hydration
- Efficient production of (R)-(-)-mandelic acid in biphasic system by immobilized recombinant E. coli被引量：29收藏
- 作者：Ni, Kefeng Wang, Hualei Zhao, Li Zhang, Minjie Zhang, Siyuan Ren, Yuhong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2013
- 关键词：Immobilization Catecholic chitosan (R)-(-)-mandelic acid Nitrilase Biphasic reaction
- Manufacturing Multienzymatic Complex Reactors In Vivo by Self-Assembly To Improve the Biosynthesis of Itaconic Acid in Escherichia coil被引量：28收藏
- 作者：Yang, Zhongwei Wang, Hongling Wang, Yuxiao Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2018
- 关键词：self-assembly PPIs MECRs itaconic acid CRISPR-Cas9
- Mechanism-Guided Computational Design of ω-Transaminase by Reprograming of High-Energy-Barrier Steps被引量：28收藏
- 作者：Yang, Lin Zhang, Kaiyue Xu, Meng Xie, Youyu Meng, Xiangqi Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2022
- 关键词：Chiral Amines Protein Engineering QM Calculation Rosetta Design omega-Transaminase
- Enhancing Alcohol Dehydrogenase Activity for the Efficient Synthesis of (S)-2-Chloro-1-(2,4-Dichlorophenyl)Ethanol Using Computer-Aided Combinational Mutagenesis被引量：27收藏
- 作者：Ye, Wenjie Xie, Jingwen Gao, Weijie Meng, Yifang Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai ZJ Bio-Tech Co.; State Key Laboratory of Bioreactor Engineering
- 来源：SSRN 2024
- 关键词：Enzyme activity - Synthesis (chemical)
- 脂肪酶 RWL 催化合成二氢燕麦生物碱 D被引量：27收藏
- 作者：Chen, Jinbo Zhai, Chuntao Jin, Minrong Wu, Qian Yuan, Minjia Wang, Xuedong Ren, Yuhong Wang, Hualei
- 机构： School of Biotechnology; Shanghai Zhina Biotechnology Co.; Shanghai Qiran Biotechnology Co.; Shanghai Jinjia Technology Co.
- 来源：[1] School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China; [2] Shanghai Zhina Biotechnology Co., Ltd., Shanghai, 201400, China; [3] Shanghai Qiran Biotechnology Co., Ltd., Shanghai, 201702, China; [4] Shanghai Jinjia Technology Co., Ltd., Shanghai, 201210, China 2026
- Switching a nitrilase from Syechocystis sp PCC6803 to a nitrile hydratase by rationally regulating reaction pathways被引量：27收藏
- 作者：Jiang, Shuiqin Zhang, Lujia Yao, Zhiqiang Gao, Bei Wang, Hualei Mao, Xiangzhao Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Ocean Univ China
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2017
- 关键词：Amides - Biochemical engineering - Enzymes
- Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system被引量：26收藏
- 作者：Yang, Zeyu Fu, Hengwei Ye, Wenjie Xie, Youyu Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2020
- 关键词：Genes - Acetone - Flow of gases - Catalyst activity - Molecular dynamics - Cloning
- Efficient Asymmetric Synthesis of Ethyl (S)-4-Chloro-3-hydroxybutyrate Using Alcohol Dehydrogenase SmADH31 with High Tolerance of Substrate and Product in a Monophasic Aqueous System被引量：25收藏
- 作者：Yang, Zeyu Ye, Wenjie Xie, Youyu Liu, Qinghai Chen, Rong Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Hangzhou Normal Univ
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2020
- 关键词：asymmetric synthesis ethyl (S)-4-chloro-3-hydroxybutanoate alcohol dehydrogenases high substrate and product tolerance monophasic aqueous system
- Development of a two-step process for production of 3-hydroxypropionic acid from glycerol using Klebsiella pneumoniae and Gluconobacter oxydans被引量：24收藏
- 作者：Zhao, Li Lin, Jinping Wang, Hualei Xie, Jingli Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2015
- 关键词：3-Hydroxypropionic acid Glycerol Klebsiella pneumoniae Gluconobacter oxydans Two-step process
- Protein Engineering of a Nitrilase from Burkholderia cenocepacia J2315 for Efficient and Enantioselective Production of (R)-o-Chloromandelic Acid被引量：23收藏
- 作者：Wang, Hualei Gao, Wenyuan Sun, Huihui Chen, Lifeng Zhang, Lujia Wang, Xuedong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2015
- 关键词：Hydrolysis - Genetic engineering - Biochemical engineering - Mutagenesis - Proteins
- Enhancing the Activity of an Alcohol Dehydrogenase by Using "Aromatic Residue Scanning" at Potential Plasticity Sites被引量：21收藏
- 作者：Ye, Wen-Jie Xie, Jing-Wen Liu, Yan Wang, Yi-Lin Zhang, Yu-Xin Yang, Xiao-Ying Yang, Lin Wang, Hua-Lei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Georgetown Preparatory Sch
- 来源：CHEMISTRY-A EUROPEAN JOURNAL 2023
- 关键词：alcohol dehydrogenase aromatic residue scanning plasticity sites protein engineering (S)-2-chloro-1-(3 4-difluorophenyl)ethanol
- Efficient Production of (R)-o-Chloromandelic Acid by Recombinant Escherichia coli Cells Harboring Nitrilase from Burkholderia cenocepacia J2315被引量：20收藏
- 作者：Wang, Hualei Sun, Huihui Gao, Wenyuan Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2014
- 关键词：Organic solvents - Escherichia coli - Ethanol - Enantiomers
- Efficient kinetic resolution of phenyl glycidyl ether by a novel epoxide hydrolase from Tsukamurella paurometabola被引量：19收藏
- 作者：Wu, Kai Wang, Hualei Sun, Huihui Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Epoxide hydrolase Enantioselectivity Phenyl glycidyl ether Kinetic resolution High substrate tolerance
- Identification and Rational Engineering of a High Substrate-Tolerant Leucine Dehydrogenase Effective for the Synthesis of L-tert-Leucine被引量：18收藏
- 作者：Meng, Xiangqi Yang, Lin Liu, Yan Wang, Hualei Shen, Yaling Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CHEMCATCHEM 2021
- 关键词：Coenzyme Catalysis Directed evolution Enzyme L-tert-leucine Leucine dehydrogenase Substrate tolerance
- A novel nitrilase from Rhodobacter sphaeroides LHS-305: cloning, heterologous expression and biochemical characterization被引量：17收藏
- 作者：Wang, Hualei Li, Guinan Li, Mingyang Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：Nitrilase Rhodobacter sphaeroides 3-Cyanopyridine Substrate specificity Regioselectivity
- Efficient production of ethyl (R)-4-chloro-3-hydroxybutanoate by a novel alcohol dehydrogenase from Lactobacillus curieae S1L19被引量：16收藏
- 作者：Zhang, Yiping Wang, Hualei Chen, Lifeng Wu, Kai Xie, Jingli Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2016
- 关键词：Cofactor Co-expression Asymmetric reduction Ethyl-(R)-4-chloro-3-hydroxybutanoate
- Development of a practical biocatalytic process for (S)-N-Boc-3-hydroxypiperidine synthesis被引量：15收藏
- 作者：Chen, Li-Feng Fan, Hai-Yang Zhang, Yi-Ping Wu, Kai Wang, Hua-Lei Lin, Jin-Ping Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：TETRAHEDRON LETTERS 2017
- 关键词：(S)-N-Boc-3-hydroxypiperidine Carbonyl reductase Asymmetric reduction Biphasic system
- Cloning, Overexpression, and Characterization of a High Enantioselective Nitrilase from Sphingomonas wittichii RW1 for Asymmetric Synthesis of (R)-Phenylglycine被引量：15收藏
- 作者：Qiu, Jian Su, Er-Zheng Wang, Hua-Lei Cai, Wen-Wen Wang, Wei Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol;Nanjing Forestry Univ
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2014
- 关键词：Nitrilase Sphingomonas wittichiiRW1 Kinetic resolution (R)-phenylglycine
- Directed evolution of nitrilase PpL19 from Pseudomonas psychrotolerans L19 and identification of enantiocomplementary mutants toward mandelonitrile被引量：15收藏
- 作者：Sun, Huihui Wang, Hualei Gao, Wenyuan Chen, Lifeng Wu, Kai Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2015
- 关键词：Directed evolution Enantiocomplementary Nitrilase Pseudomonas psychrotolerans L19
- Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase被引量：15收藏
- 作者：Chen, Li-Feng Zhang, Yi-Ping Fan, Hai-Yang Wu, Kai Lin, Jin-Ping Wang, Hua-Lei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：CATALYSIS COMMUNICATIONS 2017
- 关键词：(R)-N-Boc-3-hydroxypiperidine Carbonyl reductase Asymmetric reduction Cofactor-recycling system
- A novel nitrilase from Ralstonia eutropha H16 and its application to nicotinic acid production被引量：14收藏
- 作者：Fan, Haiyang Chen, Lifeng Sun, Huihui Wang, Hualei Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chinese Acad Fishery Sci
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Nitrilase Ralstonia eutropha Nicotinic acid Biotransformation
