成果/Result
- Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor被引量：129收藏
- 作者：Wang, Li-Juan Li, Chun-Xiu Ni, Yan Zhang, Jie Liu, Xiang Xu, Jian-He
- 机构：E China Univ Sci & Technol;Jiangnan Univ
- 来源：BIORESOURCE TECHNOLOGY 2011
- 关键词：Carbonyl reductase Streptomyces coelicolor Asymmetric reduction Ethyl (S)-4-chloro-3-hydroxybutanoate NADH regeneration
- Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity被引量：68收藏
- 作者：Ni, Yan Li, Chun-Xiu Ma, Hong-Min Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：YtbE Recombinant aldo-keto reductase Bacillus sp. Asymmetric reduction Chiral alcohols
- Efficient Synthesis of a Chiral Precursor for Angiotensin-Converting Enzyme (ACE) Inhibitors in High Space-Time Yield by a New Reductase without External Cofactors被引量：61收藏
- 作者：Shen, Nai-Dong Ni, Yan Ma, Hong-Min Wang, Li-Juan Li, Chun-Xiu Zheng, Gao-Wei Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC LETTERS 2012
- Stereoselective synthesis of L-tert-leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum被引量：58收藏
- 作者：Li, Jing Pan, Jiang Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2014
- 关键词：Leucine dehydrogenase Exiguobacterium sibiricum Reductive amination Dual enzyme co-expression L-tert-Leucine
- Stereospecific Reduction of Methyl o-Chlorobenzoylformate at 300 g?L-1 without Additional Cofactor using a Carbonyl Reductase Mined from Candida glabrata被引量：57收藏
- 作者：Ma, Hongmin Yang, Linlin Ni, Yan Zhang, Jie Li, Chun-Xiu Zheng, Gao-Wei Yang, Huaiyu Xu, Jian-He
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2012
- 关键词：bioinformatics biotransformations cofactors oxidoreductases
- Efficient Reduction of Ethyl 2-Oxo-4-phenylbutyrate at 620 g.L-1 by a Bacterial Reductase with Broad Substrate Spectrum被引量：50收藏
- 作者：Ni, Yan Li, Chun-Xiu Zhang, Jie Shen, Nai-Dong Bornscheuer, Uwe T. Xu, Jian-He
- 机构：E China Univ Sci & Technol;Ernst Moritz Arndt Univ Greifswald
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2011
- 关键词：alcohols asymmetric catalysis cofactor oxidoreductases reduction
- Highly stereoselective reduction of prochiral ketones by a bacterial reductase coupled with cofactor regeneration被引量：46收藏
- 作者：Ni, Yan Li, Chun-Xiu Wang, Li-Juan Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC & BIOMOLECULAR CHEMISTRY 2011
- 关键词：Aromatic compounds - Bacteriology - Enantiomers - Ketones
- A novel D-mandelate dehydrogenase used in three-enzyme cascade reaction for highly efficient synthesis of non-natural chiral amino acids被引量：40收藏
- 作者：Fan, Chang-Wei Xu, Guo-Chao Ma, Bao-Di Bai, Yun-Peng Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2015
- 关键词：D-Mandelate dehydrogenase L-Phenylglycine Asymmetric synthesis Cascade reaction Process optimization
- Crystal structures of Pseudomonas putida esterase reveal the functional role of residues 187 and 287 in substrate binding and chiral recognition被引量：24收藏
- 作者：Dou, Shuai Kong, Xu-Dong Ma, Bao-Di Chen, Qi Zhang, Jie Zhou, Jiahai Xu, Jian-He
- 机构：E China Univ Sci & Technol;Chinese Acad Sci;Wuhan Univ
- 来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2014
- 关键词：Crystal structure Pseudomonas putida esterase Enzyme-substrate complex Chiral recognition Activity enhancement Protein engineering
- Bioreduction of methyl o-chlorobenzoylformate at 500 g L-1 without external cofactors for efficient production of enantiopure clopidogrel intermediate被引量：22收藏
- 作者：Ni, Yan Pan, Jiang Ma, Hong-Min Li, Chun-Xiu Zhang, Jie Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：TETRAHEDRON LETTERS 2012
- 关键词：Asymmetric reduction Aldo-keto reductase Whole-cell catalyst Methyl (R)-o-chloromandelate Clopidogrel
- Relative humidity-triggered polyamide 4/Cinnamaldehyde core-shell nanofibers for antibacterial packaging被引量：16收藏
- 作者：Wang, Ziying Zhan, Fangke Zhang, Di Wang, Yibing Qiu, Yongjun Zhang, Jie Wang, Li Zhao, Liming
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI
- 来源：JOURNAL OF FOOD ENGINEERING 2023
- 关键词：Polyamide 4 Cinnamaldehyde Coaxial electrospinning RH-Triggered Active packaging
- Efficient production of ethyl (R)-2-hydroxy-4-phenylbutyrate using a cost-effective reductase expressed in Pichia pastoris被引量：8收藏
- 作者：Qian, Xiao-Long Pan, Jiang Shen, Nai-Dong Ju, Xin Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2014
- 关键词：Biocatalysis (R)-HPBE Enzyme production Recombinant Pichia pastoris Fermentation Cost-effective reductase Fed-batch culture
- Optogenetic control of Corynebacterium glutamicum gene expression被引量：8收藏
- 作者：Deng, Chen Xin, Ruijie Li, Xingjian Zhang, Jie Fan, Liqiang Qiu, Yongjun Zhao, Liming
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：NUCLEIC ACIDS RESEARCH 2024
- Novel iterative genome mining and engineering of a bifunctional KvVDH enable selective production of furan carboxylic acids from high-concentration 5-hydroxymethylfurfural被引量：0收藏
- 作者：Cui, Yanan Zhang, Jie Xing, Xijun Zhang, Hanwen Chen, Yutong Fan, Liqiang Li, Xu Qiu, Yongjun Deng, Chen Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI;East China Univ Sci & Technol
- 来源：GREEN CHEMISTRY 2026
- 关键词：Biosynthesis - Carboxylic acids - Catalysis - Catalyst activity - Computational efficiency - Conformations - Enzymes - Genes - Genome - Hydrogen bonds - Iterative methods - Screening
- Enhanced heparosan biosynthesis in Corynebacterium glutamicum by systematic metabolic engineering and translation-level fine-tuning被引量：0收藏
- 作者：Zhang, Jie Cui, Yanan Zhang, Peiyi Zhao, Mengqi Fan, Liqiang Li, Xu Deng, Chen Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI
- 来源：BIORESOURCES AND BIOPROCESSING 2026
- 关键词：Heparosan
Corynebacterium glutamicum Systematic metabolic engineering Translation-level fine-tuning Fed-batch fermentation
