成果/Result
- The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction被引量：194收藏
- 作者：Liu, Guo-Song Li, Tian Zhou, Wei Jiang, Min Tao, Xin-Yi Liu, Min Zhao, Ming Ren, Yu-Hong Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2020
- 关键词：Synthetic biology Saccharomyces cerevisiae Peroxisome Storage depot Subcellular factory Terpene
- Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction被引量：115收藏
- 作者：Zhu, Zhan-Tao Du, Meng-Meng Gao, Bei Tao, Xin-Yi Zhao, Ming Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2021
- 关键词：Saccharomyces cerevisiae Mitochondrial engineering Metabolic burden Squalene Dual engineering
- Metabolic Engineering of Saccharomyces cerevisiae To Overproduce Squalene被引量：71收藏
- 作者：Li, Tian Liu, Guo-Song Zhou, Wei Jiang, Min Ren, Yu-Hong Tao, Xin-Yi Liu, Min Zhao, Ming Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Saccharomyces cerevisiae metabolic engineering squalene overproduction ethanol carbon source utilization
- High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae被引量：57收藏
- 作者：Liu, Min Lin, Yang-Chen Guo, Jiao-Jiao Du, Meng-Meng Tao, Xinyi Gao, Bei Zhao, Ming Ma, Yushu Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2021
- 关键词：patchoulol physiological status metabolic engineering Saccharomyces cerevisiae sesquiterpene cell factories
- Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurum被引量：45收藏
- 作者：Xiong, Liang-Bin Liu, Hao-Hao Zhao, Ming Liu, Yong-Jun Song, Lu Xie, Zhi-Yong Xu, Yi-Xin Wang, Feng-Qing Wei, Dong-Zhi
- 机构：Shanghai Univ Med & Hlth Sci;East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：MICROBIAL CELL FACTORIES 2020
- 关键词：Mycolic acids Self-enhancement conversion beta-Ketoacyl-acyl carrier protein synthase Phytosterol Steroid intermediates
- Multidimensional engineering of Saccharomyces cerevisiae for the efficient production of heme by exploring the cytotoxicity and tolerance of heme被引量：20收藏
- 作者：Guo, Qidi Li, Jiacun Wang, Ming-Rui Zhao, Ming Zhang, Gege Tang, Shuyan Xiong, Liang-Bin Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2024
- 关键词：Heme Saccharomyces cerevisiae Metabolic engineering Heme cytotoxicity Laboratory evolution
- De novo design of a transcription factor for a progesterone biosensor被引量：20收藏
- 作者：Liu, Kun Zhang, Yunsen Liu, Ke Zhao, Yunqiu Gao, Bei Tao, Xinyi Zhao, Ming Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chengdu Univ Tradit Chinese Med
- 来源：BIOSENSORS & BIOELECTRONICS 2022
- 关键词：Artificial transcription factor Molecular dynamics simulation Progesterone biosensor Whole-cell Diagnosis
- A Self-Sustained System Spanning the Primary and Secondary Metabolism Stages to Boost the Productivity of Streptomyces被引量：19收藏
- 作者：Zhao, Ming Wang, Mingrui Wang, Shuiling Xiong, Liangbin Gao, Bei Liu, Min Tao, Xinyi Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：Streptomyces sigma(hrdB) PkasO* mutant metabolic regulation
- A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli被引量：19收藏
- 作者：Zhao, Ming Li, Yuting Wang, Fengqing Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Anhui Polytech Univ
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2022
- 关键词：CRISPRi Dynamic regulation Itaconic acid Biosensor
- Transformation of phytosterols into pregnatetraenedione by a combined microbial and chemical process被引量：18收藏
- 作者：Liu, Yong-Jun Ji, Wei-ting Song, Lu Tao, Xin-Yi Zhao, Ming Gao, Bei Meng, Hao Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Hunan Norchem Pharmaceut Co Ltd
- 来源：GREEN CHEMISTRY 2022
- 关键词：Alcohols - Carboxylation - Drug products - Metabolism - Metabolites - Polyols
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- A highly sensitive, selective ratiometric fluorescent probe for cobalt(II) and its applications for biological imaging被引量：15收藏
- 作者：Zhang, Shenyi Zhao, Mei Zhu, Weiping Xu, Yufang Qian, Xuhong
- 机构：E China Univ Sci & Technol
- 来源：DALTON TRANSACTIONS 2015
- 关键词：Charge transfer - Cobalt compounds - Fluorescence
- Comparison of the expression of phospholipase D from Streptomyces halstedii in different hosts and its over-expression in Streptomyces lividans被引量：14收藏
- 作者：Tao, Xinyi Zhao, Ming Zhang, Ying Liu, Min Liu, Qinghai Wang, Wei Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：FEMS MICROBIOLOGY LETTERS 2019
- 关键词：phospholipase D Streptomyces lividans Pichia pastoris cytotoxic dual-promoter
- Engineering diverse eubacteria promoters for robust Gene expression in Streptomyces lividans被引量：14收藏
- 作者：Zhao, Ming Wang, Shui-Ling Tao, Xin-Yi Zhao, Guo-Ling Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2019
- 关键词：Streptomyces Heterologous promoter 5 '-UTR RBS sigma hrdB
- One-pot biosynthesis of 7β-hydroxyan drost-4-ene-3,17-dione from phytosterols by cofactor regeneration system in engineered mycolicibacterium neoaurum被引量：12收藏
- 作者：Zhao, Yun-Qiu Liu, Yong-Jun Ji, Wei-Ting Liu, Kun Gao, Bei Tao, Xin-Yi Zhao, Ming Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2022
- 关键词：7 beta-hydroxyandrost-4-ene-3,17-dione Phytosterols mP450-BM3 Nicotinamide adenine dinucleotide phosphate regeneration Mycolicibacterium
- Engineering Mycolicibacterium neoaurum for the production of antioxidant ergothioneine被引量：12收藏
- 作者：Xiong, Liang-Bin Xie, Zhi-Yong Ke, Jie Wang, Li Gao, Bei Tao, Xin-Yi Zhao, Ming Shen, Ya-Ling Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Huawei Safety Evaluat & Med Res Shanghai Co Ltd
- 来源：FOOD BIOENGINEERING 2022
- 关键词：endogenous metabolic engineering ergothioneine Mycolicibacterium neoaurum S-adenosyl-L-methionine regeneration
- Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis被引量：12收藏
- 作者：Xiong, Liang-Bin Liu, Hao-Hao Song, Lu Dong, Miao-Miao Ke, Jie Liu, Yong-Jun Liu, Ke Zhao, Ming Wang, Feng-Qing Wei, Dong-Zhi
- 机构：Shanghai Univ Med & Hlth Sci;East China Univ Sci & Technol;Huawei Safety Evaluat & Med Res Shanghai Co Ltd
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2022
- 关键词：Mycolicibacterium 9-OHAD Cell permeability Soybean phytosterol fbpC3 embC
- Photocontrol of Itaconic Acid Synthesis in Escherichia coli被引量：12收藏
- 作者：Li, Yuting Zhao, Ming Wei, Dongzhi Zhang, Jian Ren, Yuhong
- 机构：East China Univ Sci & Technol;Anhui Polytech Univ
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词： itaconic acid isocitrate dehydrogenase photoswitch AsLOV2 metabolism regulation
- Establishment of a low-dosage-IPTG inducible expression system construction method in Escherichia coli被引量：10收藏
- 作者：Zhao, Ming Tao, Xin-Yi Wang, Feng-Qing Ren, Yu-Hong Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BASIC MICROBIOLOGY 2018
- 关键词：gene expression system lac operon low-dosage-IPTG synthetic promoter library
- Original Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system被引量：7收藏
- 作者：Liu, Ke Lin, Gui-Hong Liu, Kun Liu, Yong-Jun Tao, Xin-Yi Gao, Bei Zhao, Ming Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2022
- 关键词：Site-specific recombination Phage integrase Xer recombinases Mycolicibacterium Multi-copy integration
- CRISPR-Cas Assisted Shotgun Mutagenesis Method for EvolutionaryGenome Engineering被引量：7收藏
- 作者：Zhao, Ming Gao, Miaomiao Xiong, Liangbin Liu, Yongjun Tao, Xinyi Gao, Bei Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Anhui Polytech Univ;Shanghai Univ Med & Hlth Sci
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：CRISPR-mediated genome engineering whole-genome mutagenesis iterative evolution
- A CRISPRi mediated self-inducible system for dynamic regulation of TCA cycle and improvement of itaconic acid production in Escherichia coli被引量：2收藏
- 作者：Ming Zhao Yuting Li Fengqing Wang Yuhong Ren Dongzhi Wei
- 机构：State Key Lab of Bioreactor Engineering;Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding
- 来源：合成和系统生物技术（英文） 2022
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- Improving the biotransformation efficiency of soybean phytosterols in Mycolicibacterium neoaurum by the combined deletion of fbpC3 and embC in cell envelope synthesis被引量：2收藏
- 作者：Liang-Bin Xiong Hao-Hao Liu Lu Song Miao-Miao Dong Jie Ke Yong-Jun Liu Ke Liu Ming Zhao Feng-Qing Wang Dong-Zhi Wei
- 机构：Jiading District Central Hospital Affiliated Shanghai University of Medicine and Health Sciences;State Key Laboratory of Bioreactor Engineering;Huawei Safety Evaluation&Medical Research(Shanghai)Co.
- 来源：合成和系统生物技术（英文） 2022
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- Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system被引量：1收藏
- 作者：Ke Liu Gui-Hong Lin Kun Liu Yong-Jun Liu Xin-Yi Tao Bei Gao Ming Zhao Dong-Zhi Wei Feng-Qing Wang
- 机构：State Key Laboratory of Bioreactor Engineering
- 来源：合成和系统生物技术（英文） 2022
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- 影响α-淀粉酶分离液澄清度的因素被引量：0收藏
- 作者：朱树新 包静 赵铭
- 机构：华东化工学院生化工程研究所
- 来源：《华东化工学院学报》 1989
- 关键词：酶 淀粉酶 絮凝 分离 预处理
- 摘要：本文根据凝聚和絮凝原理,研究了无机盐和高分子絮凝剂加入量对枯草杆菌BF 7658α-淀粉酶分离液澄清度的影响。实验结果表明,氯化钙可使α-淀粉酶发酵液的ζ电位降低,分离液的澄清度增加,用氯化钙和磷酸氢二钠来处理发酵液,则...
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- 超滤法纯化超氧物岐化酶被引量：0收藏
- 作者：赵铭 袁勤生 朱树新
- 机构：华东化工学院生化工程研究所
- 来源：《华东化工学院学报》 1991
- 关键词：歧化酶 提纯 过滤膜
- 摘要：研制了适合纯化猪血超氧物岐化酶(Cu.Zn-SOD)溶液的醋酸纤维素(CA)超滤膜,其截留分子量约为16 000。研究了溶液浓度、操作压力等因素对通量的影响。结果表明,自制CA膜超滤纯化SOD溶液性能优于一些市售膜。收率...
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- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：0收藏
- 作者：Bing-Yao Sun Hua-Lu Sui Zi-Wei Liu Xin-Yi Tao Bei Gao Ming Zhao Yu-Shu Ma Jian Zhao Min Liu Feng-Qing Wang Dong-Zhi Wei
- 机构：State Key Lab of Bioreactor Engineering
- 来源：生物资源与生物加工(英文) 2022
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