成果/Result
- Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus for baking industry and its magnetic immobilization被引量：42收藏
- 作者：He, Lei Mao, Youzhi Zhang, Lujia Wang, Hualei Alias, Siti Aisyah Gao, Bei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Univ Malaya
- 来源：BMC BIOTECHNOLOGY 2017
- 关键词：alpha-Amylase Antarctic fungus Biochemical properties Bread quality Immobilization
- Process Development for the Production of (R)-(-)-Mandelic Acid by Recombinant Escherichia coli Cells Harboring Nitrilase from Burkholderia cenocepacia J2315被引量：36收藏
- 作者：Wang, Hualei Fan, Haiyang Sun, Huihui Zhao, Li Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2015
- 关键词：Cost effectiveness - Organic acids - Hydrolysis
- Efficient production of (R)-(-)-mandelic acid in biphasic system by immobilized recombinant E. coli被引量：32收藏
- 作者：Ni, Kefeng Wang, Hualei Zhao, Li Zhang, Minjie Zhang, Siyuan Ren, Yuhong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Biotechnology 2013
- 关键词：Hydrolysis - Molecular biology - Solutions - Organic acids - Cell culture - Iron oxides - Escherichia coli - Cells
- Combinatorial Evolution of Site-Guided Function Switch of Nitrilase to Nitrile Hydratase: Alleviating the Trade-Off between Hydration Activity and Specific Activity被引量：31收藏
- 作者：Sun, Yangyang Tang, Zhuzhu Pan, Tingze Zhang, Ke Wang, Liuzhu Zhai, Xingye Jia, Yiran Yuan, Tianqi Qian, Yujia Wang, Hualei Wei, Dongzhi Yang, Shengli
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：SSRN 2023
- 关键词：Economic and social effects - Hydration
- Mechanism-Guided Computational Design of ω-Transaminase by Reprograming of High-Energy-Barrier Steps被引量：28收藏
- 作者：Yang, Lin Zhang, Kaiyue Xu, Meng Xie, Youyu Meng, Xiangqi Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2022
- 关键词：Chiral Amines ProtEIn Engineering QM Calculation Rosetta Design omega-Transaminase
- Enhancing Alcohol Dehydrogenase Activity for the Efficient Synthesis of (S)-2-Chloro-1-(2,4-Dichlorophenyl)Ethanol Using Computer-Aided Combinational Mutagenesis被引量：27收藏
- 作者：Ye, Wenjie Xie, Jingwen Gao, Weijie Meng, Yifang Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai ZJ Bio-Tech Co.; State Key Laboratory of Bioreactor Engineering
- 来源：SSRN 2024
- 关键词：Enzyme activity - Synthesis (chemical)
- 脂肪酶 RWL 催化合成二氢燕麦生物碱 D被引量：27收藏
- 作者：Chen, Jinbo Zhai, Chuntao Jin, Minrong Wu, Qian Yuan, Minjia Wang, Xuedong Ren, Yuhong Wang, Hualei
- 机构： School of Biotechnology; Shanghai Zhina Biotechnology Co.; Shanghai Qiran Biotechnology Co.; Shanghai Jinjia Technology Co.
- 来源：[1] School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China; [2] Shanghai Zhina Biotechnology Co., Ltd., Shanghai, 201400, China; [3] Shanghai Qiran Biotechnology Co., Ltd., Shanghai, 201702, China; [4] Shanghai Jinjia Technology Co., Ltd., Shanghai, 201210, China 2026
- Switching a nitrilase from Syechocystis sp PCC6803 to a nitrile hydratase by rationally regulating reaction pathways被引量：27收藏
- 作者：Jiang, Shuiqin Zhang, Lujia Yao, Zhiqiang Gao, Bei Wang, Hualei Mao, Xiangzhao Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Ocean Univ China
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2017
- 关键词：Amides - Biochemical engineering - Enzymes
- Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system被引量：26收藏
- 作者：Yang, Zeyu Fu, Hengwei Ye, Wenjie Xie, Youyu Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2020
- 关键词：Genes - Acetone - Flow of gases - Catalyst activity - Molecular dynamics - Cloning
- Efficient Asymmetric Synthesis of Ethyl (S)-4-Chloro-3-hydroxybutyrate Using Alcohol Dehydrogenase SmADH31 with High Tolerance of Substrate and Product in a Monophasic Aqueous System被引量：25收藏
- 作者：Yang, Zeyu Ye, Wenjie Xie, Youyu Liu, Qinghai Chen, Rong Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Hangzhou Normal Univ
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2020
- 关键词：asymmetric synthesis ethyl (S)-4-chloro-3-hydroxybutanoate alcohol dehydrogenases high substrate and product tolerance monophasic aqueous system
- ProtEIn Engineering of a Nitrilase from Burkholderia cenocepacia J2315 for Efficient and Enantioselective Production of (R)-o-Chloromandelic Acid被引量：23收藏
- 作者：Wang, Hualei Gao, Wenyuan Sun, Huihui Chen, Lifeng Zhang, Lujia Wang, Xuedong Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2015
- 关键词：Hydrolysis - Genetic engineering - Biochemical engineering - Mutagenesis - ProtEIns
- Enhancing the Activity of an Alcohol Dehydrogenase by Using "Aromatic Residue Scanning" at Potential Plasticity Sites被引量：21收藏
- 作者：Ye, Wen-Jie Xie, Jing-Wen Liu, Yan Wang, Yi-Lin Zhang, Yu-Xin Yang, Xiao-Ying Yang, Lin Wang, Hua-Lei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Georgetown Preparatory Sch
- 来源：CHEMISTRY-A EUROPEAN JOURNAL 2023
- 关键词：alcohol dehydrogenase aromatic residue scanning plasticity sites protEIn engineering (S)-2-chloro-1-(3 4-difluorophenyl)ethanol
- Efficient Production of (R)-o-Chloromandelic Acid by Recombinant Escherichia coli Cells Harboring Nitrilase from Burkholderia cenocepacia J2315被引量：20收藏
- 作者：Wang, Hualei Sun, Huihui Gao, Wenyuan Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2014
- 关键词：Organic solvents - Escherichia coli - Ethanol - Enantiomers
- Efficient kinetic resolution of phenyl glycidyl ether by a novel epoxide hydrolase from Tsukamurella paurometabola被引量：19收藏
- 作者：Wu, Kai Wang, Hualei Sun, Huihui Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Epoxide hydrolase Enantioselectivity Phenyl glycidyl ether Kinetic resolution High substrate tolerance
- Identification and Rational Engineering of a High Substrate-Tolerant Leucine Dehydrogenase Effective for the Synthesis of L-tert-Leucine被引量：18收藏
- 作者：Meng, Xiangqi Yang, Lin Liu, Yan Wang, Hualei Shen, Yaling Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CHEMCATCHEM 2021
- 关键词：Coenzyme Catalysis Directed evolution Enzyme L-tert-leucine Leucine dehydrogenase Substrate tolerance
- A novel nitrilase from Rhodobacter sphaeroides LHS-305: cloning, heterologous expression and biochemical characterization被引量：17收藏
- 作者：Wang, Hualei Li, Guinan Li, Mingyang Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：Nitrilase Rhodobacter sphaeroides 3-Cyanopyridine Substrate specificity Regioselectivity
- Efficient production of ethyl (R)-4-chloro-3-hydroxybutanoate by a novel alcohol dehydrogenase from Lactobacillus curieae S1L19被引量：16收藏
- 作者：Zhang, Yiping Wang, Hualei Chen, Lifeng Wu, Kai Xie, Jingli Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2016
- 关键词：Cofactor Co-expression Asymmetric reduction Ethyl-(R)-4-chloro-3-hydroxybutanoate
- Cloning, Overexpression, and Characterization of a High Enantioselective Nitrilase from Sphingomonas wittichii RW1 for Asymmetric Synthesis of (R)-Phenylglycine被引量：15收藏
- 作者：Qiu, Jian Su, Er-Zheng Wang, Hua-Lei Cai, Wen-Wen Wang, Wei Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol;Nanjing Forestry Univ
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2014
- 关键词：Nitrilase Sphingomonas wittichiiRW1 Kinetic resolution (R)-phenylglycine
- Efficient bioreductive production of (R)-N-Boc-3-hydroxypiperidine by a carbonyl reductase被引量：15收藏
- 作者：Chen, Li-Feng Zhang, Yi-Ping Fan, Hai-Yang Wu, Kai Lin, Jin-Ping Wang, Hua-Lei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：CATALYSIS COMMUNICATIONS 2017
- 关键词：(R)-N-Boc-3-hydroxypiperidine Carbonyl reductase Asymmetric reduction Cofactor-recycling system
- Characterization of a novel dextran produced by Gluconobacter oxydans DSM 2003被引量：14收藏
- 作者：Wang, Shu Mao, Xiangzhao Wang, Hualei Lin, Jinping Li, Fuli Wei, Dongzhi
- 机构：Chinese Acad Sci;Ocean Univ China;E China Univ Sci & Technol;Chinese Acad Sci
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：Dextran Gluconobacter oxydans Monosaccharide composition Structure analysis
- Cloning, purification and evaluation of the enzymatic properties of a novel arylacetonitrilase from Luminiphilus syltensis NOR5-1B: a potential biocatalyst for the synthesis of mandelic acid and its derivatives被引量：14收藏
- 作者：Sun, Huihui Gao, Wenyuan Fan, Haiyang Wang, Hualei Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2015
- 关键词：Arylacetonitrilase (R)-o-Chloromandelic acid Luminiphilus syltensis Mandelonitrile derivatives Nitrilase
- Enhancement of ethyl (S)-4-chloro-3-hydroxybutanoate production at high substrate concentration by in situ resin adsorption被引量：14收藏
- 作者：Chen, Li-Feng Fan, Hai-Yang Zhang, Yi-Ping Wei, Wei Lin, Jin-Ping Wei, Dong-Zhi Wang, Hua-Lei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Ethyl (S)-4-chloro-3-hydroxybutanoate Carbonyl reductase Asymmetric reduction Product inhibition Resin adsorption
- Efficient Biocatalytic Synthesis of (R)-2-Chloro-1-(3,4-difluorophenyl)ethanol by the Short-Chain Dehydrogenase PpKR8 from Paraburkholderia phymatum STM815被引量：14收藏
- 作者：He, Ling Ye, Wenjie Xie, Youyu Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2022
- 关键词：(R)-2-chloro-1-(3,4-difluorophenyl)ethanol alcohol dehydrogenase asymmetric reduction coexpression
- A novel nitrilase from Ralstonia eutropha H16 and its application to nicotinic acid production被引量：14收藏
- 作者：Fan, Haiyang Chen, Lifeng Sun, Huihui Wang, Hualei Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chinese Acad Fishery Sci
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Nitrilase Ralstonia eutropha Nicotinic acid Biotransformation
- Protopectinase production by Paenibacillus polymyxa Z6 and its application in pectin extraction from apple pomace被引量：14收藏
- 作者：Zhang, Jian Zhao, Li Gao, Bei Wei, Wei Wang, Hualei Xie, Jingli
- 机构：East China Univ Sci & Technol;SCICB
- 来源：JOURNAL OF FOOD PROCESSING AND PRESERVATION 2018
- 关键词：Fruits - Moisture - Energy utilization - Fermentation - Viscosity
- Characterization of a novel nitrilase, BGC4, from Paraburkholderia graminis showing wide-spectrum substrate specificity, a potential versatile biocatalyst for the degradation of nitriles被引量：13收藏
- 作者：Fan, Haiyang Chen, Lifeng Sun, Huihui Wang, Hualei Liu, Qinghai Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chinese Acad Fishery Sci Qingdao
- 来源：BIOTECHNOLOGY LETTERS 2017
- 关键词：Biodegradation Nitrilase Paraburkholderia graminis Wide-spectrum substrate specificity
- Practical two-step synthesis of enantiopure styrene oxide through an optimized chemoenzymatic approach被引量：12收藏
- 作者：Wu, Kai Wang, Hualei Chen, Lifeng Fan, Haiyang Zhao, Zhiqiang Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Styrene oxide Alcohol dehydrogenase Enantioselectivity Directed evolution
- Highly efficient synthesis of pharmaceutically relevant chiral 3-N-substi-tuted-azacyclic alcohols using two enantiocomplementary short chain dehydrogenases被引量：12收藏
- 作者：Yang, Lin Zhang, Yiping Liu, Yan Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Dongchang Middle Sch
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2022
- 关键词：Chiral 3-N-substituted azacyclic alcohols Alcohol dehydrogenase Bioreduction short chain dehydrogenases N-Boc-3-piperidone
- Significantly enhancing the stereoselectivity of a regioselective nitrilase for the production of (S)-3-cyano-5-methylhexanoic acid using an MM/PBSA method被引量：11收藏
- 作者：Chen, Zhi Wang, Hualei Yang, Lin Jiang, Shuiqing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CHEMICAL COMMUNICATIONS 2021
- 关键词：Binding energy - Molecular dynamics - Free energy - Hydrogen bonds
- Rational engineering of Acinetobacter tandoii glutamate dehydrogenase for asymmetric synthesis of l-homoalanine through biocatalytic cascades被引量：11收藏
- 作者：Wang, Liuzhu Diao, Shiqing Sun, Yangyang Jiang, Shuiqin Liu, Yan Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2021
- 关键词：Amino acids - Substrates - Catalyst activity - Efficiency - Mass transfer - Hydrophobicity
