成果/Result
- The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction被引量：194收藏
- 作者：Liu, Guo-Song Li, Tian Zhou, Wei Jiang, Min Tao, Xin-Yi Liu, Min Zhao, Ming Ren, Yu-Hong Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2020
- 关键词：Synthetic biology Saccharomyces cerevisiae Peroxisome Storage depot Subcellular factory Terpene
- Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction被引量：115收藏
- 作者：Zhu, Zhan-Tao Du, Meng-Meng Gao, Bei Tao, Xin-Yi Zhao, Ming Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2021
- 关键词：Saccharomyces cerevisiae Mitochondrial engineering Metabolic burden Squalene Dual engineering
- Metabolic Engineering of Saccharomyces cerevisiae To Overproduce Squalene被引量：71收藏
- 作者：Li, Tian Liu, Guo-Song Zhou, Wei Jiang, Min Ren, Yu-Hong Tao, Xin-Yi Liu, Min Zhao, Ming Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Saccharomyces cerevisiae metabolic engineering squalene overproduction ethanol carbon source utilization
- Simple and Cheap CRISPR/Cas12a Biosensor Based on Plug-and-Play of DNA Aptamers for the Detection of Endocrine-Disrupting Compounds被引量：65收藏
- 作者：Wang, Feng-Qing Zhao, Yunqiu Zhu, Lin Ding, Yaxue Ji, Weiting Liu, Kun Liu, Ke Gao, Bei Tao, Xinyi Dong, Yu-Guo Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; Key Laboratory of Biocatalysis and Intelligent Manufacturing
- 来源：SSRN 2023
- 关键词：DNA - Endocrine disrupters - Phenols
- Functional expression of a novel α-amylase from Antarctic psychrotolerant fungus for baking industry and its magnetic immobilization被引量：42收藏
- 作者：He, Lei Mao, Youzhi Zhang, Lujia Wang, Hualei Alias, Siti Aisyah Gao, Bei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Univ Malaya
- 来源：BMC BIOTECHNOLOGY 2017
- 关键词：alpha-Amylase Antarctic fungus Biochemical properties Bread quality Immobilization
- Engineering Saccharomyces cerevisiae for Hyperproduction of β-Amyrin by Mitigating the Inhibition Effect of Squalene on β-Amyrin Synthase被引量：35收藏
- 作者：Du, Meng-Meng Zhu, Zhan-Tao Zhang, Ge-Ge Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Liu, Min Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：beta-amyrin squalene inhibition peroxisome compartmentalization competition pathway Saccharomyces cerevisiae
- High-yield porphyrin production through metabolic engineering and biocatalysis被引量：34收藏
- 作者：Chen, Haihong Wang, Yaohong Wang, Weishan Cao, Ting Zhang, Lu Wang, Zhengduo Chi, Xuran Shi, Tong Wang, Huangwei He, Xinwei Liang, Mindong Yang, Mengxue Jiang, Wenyi Lv, Dongyuan Yu, Jiaming Zhu, Guoliang Xie, Yongtao Gao, Bei Wang, Xinye Liu, Xueting Li, Youyuan Ouyang, Limin Zhang, Jingyu Liu, Huimin Li, Zilong Tong, Yaojun Xia, Xuekui Tan, Gao-Yi Zhang, Lixin
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ;Qilu Univ Technol
- 来源：NATURE BIOTECHNOLOGY 2025
- 关键词：Catalysis - Diagnosis - Fermentation - Medical applications - Metabolic engineering
- Improving the catalytic activity of lipase LipK107 from Proteus sp. by site-directed mutagenesis in the lid domain based on computer simulation被引量：34收藏
- 作者：Gao, Bei Xu, Tao Lin, Jinping Zhang, Lujia Su, Erzheng Jiang, Zhengbing Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; College of Life Science
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Lipases - Palmitic acid - Mutagenesis - Catalysis
- Break Through the Thermostability of Glucose Oxidase in Extremely Thermal Environments with a Novel Dynamic Ensemble Design Protocol被引量：34收藏
- 作者：Zhang, Lujia Miao, Tingwei Zhi, Fengdong Yang, Xin Yuan, Zhaoting Zhang, Chuanxi Feng, Yinghui Wei, Hao Gao, Bei
- 机构： Shanghai Engineering Research Center of Molecular Therapeutics & New Drug Development; School of Biotechnology; NYU-ECNU Center for Computational Chemistry at NYU Shanghai; Department of Micro/Nano Electronics
- 来源：SSRN 2024
- 关键词：Computational methods - Design - Economic and social effects - Enzyme activity - Glucose - Glucose oxidase - Glucose sensors - Molecular dynamics
- Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production被引量：33收藏
- 作者：Gao, Bei Su, Erzheng Lin, Jinping Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Biotechnology 2009
- 关键词：Catalysts - Cloning - Cytology - Gene encoding - Temperature - Biodiesel - Purification - Alkalinity
- Umami polypeptide detection system targeting the human T1R1 receptor and its taste-presenting mechanism被引量：32收藏
- 作者：Zhang, Chuanxi Miao, Yulu Feng, Yinghui Wang, Jiawei Tian, Zhuoli Dong, Juan Gao, Bei Zhang, Lujia
- 机构：East China Normal Univ;East China Univ Sci & Technol;NYU Shanghai;Shanghai Jiao Tong Univ;Univ Shanghai Sci & Tecchnol
- 来源：BIOMATERIALS 2022
- 关键词：Umami petides hT1R1 receptor Interaction Detection system
- In vivo functional expression of a screened P. aeruginosa chaperone-dependent lipase in E. coli被引量：29收藏
- 作者：Wu, Xiangping You, Pengyong Su, Erzheng Xu, Jingjing Gao, Bei Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：BMC Biotechnology 2012
- 关键词：Alcohols - DNA - Escherichia coli - Esterification - Esters - Genes - Lipases
- Cloning of a novel thermostable glucoamylase from thermophilic fungus Rhizomucor pusillus and high-level co-expression with a-amylase in Pichia pastoris被引量：27收藏
- 作者：He, Zhenggui Zhang, Lujia Mao, Youzhi Gu, Jingchao Pan, Qi Zhou, Sixing Gao, Bei Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：BMC Biotechnology 2014
- 关键词：Starch - Xylose - Maltose - Glucose - Hydrolysis - Purification - Cloning - Yeast - Gene encoding - Amylases
- Switching a nitrilase from Syechocystis sp PCC6803 to a nitrile hydratase by rationally regulating reaction pathways被引量：27收藏
- 作者：Jiang, Shuiqin Zhang, Lujia Yao, Zhiqiang Gao, Bei Wang, Hualei Mao, Xiangzhao Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Ocean Univ China
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2017
- 关键词：Amides - Biochemical engineering - Enzymes
- Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface被引量：26收藏
- 作者：Jiang, Zhengbing Gao, Bei Ren, Ren Tao, Xingyi Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; College of Life Science
- 来源：BMC Biotechnology 2008
- 关键词：Biocatalysts - Esters - Flocculation - Genes - Hydrolysis - Phenols - Transesterification - Yeast
- De novo design of a transcription factor for a progesterone biosensor被引量：20收藏
- 作者：Liu, Kun Zhang, Yunsen Liu, Ke Zhao, Yunqiu Gao, Bei Tao, Xinyi Zhao, Ming Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chengdu Univ Tradit Chinese Med
- 来源：BIOSENSORS & BIOELECTRONICS 2022
- 关键词：Artificial transcription factor Molecular dynamics simulation Progesterone biosensor Whole-cell Diagnosis
- Multidimensional engineering of Saccharomyces cerevisiae for the efficient production of heme by exploring the cytotoxicity and tolerance of heme被引量：20收藏
- 作者：Guo, Qidi Li, Jiacun Wang, Ming-Rui Zhao, Ming Zhang, Gege Tang, Shuyan Xiong, Liang-Bin Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2024
- 关键词：Heme Saccharomyces cerevisiae Metabolic engineering Heme cytotoxicity Laboratory evolution
- Boosting the epoxidation of squalene to produce triterpenoids in Saccharomyces cerevisiae被引量：20收藏
- 作者：Du, Meng-Meng Zhang, Ge-Ge Zhu, Zhan-Tao Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 2023
- 关键词：2,3-Oxidosqualene 2,3:22,23-Dioxidosqualene Triterpenoid ERG7 Two-stage fermentation
- Construction of a whole-cell catalyst displaying a fungal lipase for effective treatment of oily wastewaters被引量：19收藏
- 作者：Song, Huiting Zhou, Lichao Zhang, Lujia Gao, Bei Wei, Dongzhi Shen, Yaling Wang, Rui Madzak, Catherine Jiang, Zhengbing
- 机构：Hubei Univ;E China Univ Sci & Technol;Hubei Univ;AgroParistech
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：Activated sludge Bioremediation Cell surface display Lipase Oily wastewater Yarrowia lipolytica
- Development of a novel Aspergillus uracil deficient expression system and its application in expressing a cold-adapted α-amylase gene from Antarctic fungi Geomyces pannorum被引量：18收藏
- 作者：Mao, Youzhi Yin, Yanchen Zhang, Lujia Alias, Siti Aisyah Gao, Bei Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Univ Malaya
- 来源：PROCESS BIOCHEMISTRY 2015
- 关键词：Cold-adapted alpha-amylase pyrF Aspergillus oryzae Geomyces pannorum Heterologous expression
- Transformation of phytosterols into pregnatetraenedione by a combined microbial and chemical process被引量：18收藏
- 作者：Liu, Yong-Jun Ji, Wei-ting Song, Lu Tao, Xin-Yi Zhao, Ming Gao, Bei Meng, Hao Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Hunan Norchem Pharmaceut Co Ltd
- 来源：GREEN CHEMISTRY 2022
- 关键词：Alcohols - Carboxylation - Drug products - Metabolism - Metabolites - Polyols
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- Engineering Komagataella phaffii to produce lycopene sustainably from glucose or methanol被引量：15收藏
- 作者：Zhou, Wei Ling, Rui-Jing Yang, Yi-Chen Hou, Shu-Ting Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2025
- 关键词：Komagataella phaffii Metabolic engineering Dynamic regulation Transcriptome analysis Methanol Lycopene
- Protopectinase production by Paenibacillus polymyxa Z6 and its application in pectin extraction from apple pomace被引量：14收藏
- 作者：Zhang, Jian Zhao, Li Gao, Bei Wei, Wei Wang, Hualei Xie, Jingli
- 机构：East China Univ Sci & Technol;SCICB
- 来源：JOURNAL OF FOOD PROCESSING AND PRESERVATION 2018
- 关键词：Fruits - Moisture - Energy utilization - Fermentation - Viscosity
- Light-driven progesterone production by InP-(M. neoaurum) biohybrid system被引量：14收藏
- 作者：Liu, Kun Wang, Feng-Qing Liu, Ke Zhao, Yunqiu Gao, Bei Tao, Xinyi Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Synthetic biology Progesterone P450 Electron transfer InP nanoparticles NADPH/NADP(+)
- Engineering Mycolicibacterium neoaurum for the production of antioxidant ergothionEIne被引量：12收藏
- 作者：Xiong, Liang-Bin Xie, Zhi-Yong Ke, Jie Wang, Li Gao, Bei Tao, Xin-Yi Zhao, Ming Shen, Ya-Ling Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Huawei Safety Evaluat & Med Res Shanghai Co Ltd
- 来源：FOOD BIOENGINEERING 2022
- 关键词：endogenous metabolic engineering ergothionEIne Mycolicibacterium neoaurum S-adenosyl-L-methionine regeneration
- An Energy Optimization Strategy Based on the Perfect Conformation of Prolyl Endopeptidase for Improving Catalytic Efficiency被引量：12收藏
- 作者：Li, Xiaolin Cong, Yalong Ma, Mingzhe You, Zhi-Neng Gao, Bei Zhang, John Z. H. Zhang, Lujia
- 机构：East China Normal Univ;NYU;NYU Shanghai;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：protEIn engineering prolyl endopeptidase molecular dynamics simulations site-directed mutagenesis catalytic efficiency
- Identification and magnetic immobilization of a pyrophilous aspartic protease from Antarctic psychrophilic fungus被引量：10收藏
- 作者：Gao, Bei He, Lei Wei, Dongzhi Zhang, Lujia
- 机构：East China Univ Sci & Technol;East China Normal Univ;NYU Shanghai
- 来源：JOURNAL OF FOOD BIOCHEMISTRY 2018
- 关键词：aspartic protease bond specificity cheese-making magnetic immobilization
- A novel saccharifying α-amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization被引量：10收藏
- 作者：Gao, Bei Mao, Youzhi Zhang, Lujia He, Lei Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：STARCH-STARKE 2016
- 关键词：Geomyces pannorum High-glucose-producing alpha-amylase Pichia pastoris Thermophilic
- CRISPR/Cas9-based toolkit for rapid marker recycling and combinatorial libraries in Komagataella phaffii被引量：10收藏
- 作者：Zhou, Wei Li, Yuanyi Liu, Guosong Qin, Weichuang Wei, Dongzhi Wang, Fengqing Gao, Bei
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2024
- 关键词：Komagataella phaffii CRISPR/Cas9 Visual marker recycling Multi-fragment integration Large DNA fragment knockout Yeast assembled libraries
