成果/Result
- The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction被引量：194收藏
- 作者：Liu, Guo-Song Li, Tian Zhou, Wei Jiang, Min Tao, Xin-Yi Liu, Min Zhao, Ming Ren, Yu-Hong Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2020
- 关键词：Synthetic biology Saccharomyces cerevisiae Peroxisome Storage depot Subcellular factory Terpene
- Metabolic Engineering of Saccharomyces cerevisiae To Overproduce Squalene被引量：71收藏
- 作者：Li, Tian Liu, Guo-Song Zhou, Wei Jiang, Min Ren, Yu-Hong Tao, Xin-Yi Liu, Min Zhao, Ming Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Saccharomyces cerevisiae metabolic engineering squalene overproduction ethanol carbon source utilization
- High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae被引量：57收藏
- 作者：Liu, Min Lin, Yang-Chen Guo, Jiao-Jiao Du, Meng-Meng Tao, Xinyi Gao, Bei Zhao, Ming Ma, Yushu Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2021
- 关键词：patchoulol physiological status metabolic engineering Saccharomyces cerevisiae sesquiterpene cell factories
- Significantly Enhanced Production of Patchoulol in Metabolically Engineered Saccharomyces cerevisiae被引量：53收藏
- 作者：Ma, Bin Liu, Min Li, Zhen-Hai Tao, Xinyi Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：patchoulol sesquiterpene metabolic engineering Saccharomyces cerevisiae terpenoids
- Metabolic engineering of Pichia pastoris for the production of dammarenediol-II被引量：51收藏
- 作者：Liu, Xin-Bin Liu, Min Tao, Xin-Yi Zhang, Zhong-Xi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2015
- 关键词：Dammarenediol-II Metabolic engineering Pichia pastoris Ginsenosides 2,3-Oxidosqualene
- Engineered 3-Ketosteroid 9α-Hydroxylases in Mycobacterium neoaurum: an Efficient Platform for Production of Steroid Drugs被引量：36收藏
- 作者：Liu, Hao-Hao Xu, Li-Qin Yao, Kang Xiong, Liang-Bin Tao, Xin-Yi Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2018
- 关键词：3-ketosteroid-9 alpha-hydroxylase androst-1,4-diene-3,17-dione (ADD) 9 alpha-hydroxy-4-androstene-3,17-dione (9-OHAD) mycobacterium Mn25795 sterols 9 alpha-hydroxy-4-androstene-3,17-dione androst-1,4-diene-3,17-dione sterol
- Engineering Saccharomyces cerevisiae for Hyperproduction of β-Amyrin by Mitigating the Inhibition Effect of Squalene on β-Amyrin Synthase被引量：35收藏
- 作者：Du, Meng-Meng Zhu, Zhan-Tao Zhang, Ge-Ge Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Liu, Min Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：beta-amyrin squalene inhibition peroxisome compartmentalization competition pathway Saccharomyces cerevisiae
- Metagenomic sequencing reveals microbial gene catalogue of phosphinothricin-utilized soils in South China被引量：29收藏
- 作者：Lou, Jiali Liu, Min Gu, Jiali Liu, Qinghai Zhao, Li Ma, Yushu Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Huzhou Univ
- 来源：GENE 2019
- 关键词：Phosphinothricin N-acetyltransferase Metagenomics Antibiotic resistance genes Virulence factors Microbial community
- Increasing L-homoserine production in Escherichia coli by engineering the central metabolic pathways被引量：25收藏
- 作者：Liu, Min Lou, Jiali Gu, Jiali Lyu, Xiao-Mei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Huzhou Univ;Nanyang Technol Univ
- 来源：JOURNAL OF BIOTECHNOLOGY 2020
- 关键词：L-Homoserine Central metabolic pathways Metabolic engineering Escherichia coli
- Integrated Transcriptome and Proteome Studies Reveal the Underlying Mechanisms for Sterol Catabolism and Steroid Production in Mycobacterium neoaurum被引量：24收藏
- 作者：Liu, Min Xiong, Liang-Bin Tao, Xinyi Liu, Qing-Hai Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：sterol catabolism transcriptome proteome Mycobacterium
- Design and evaluation of a phospholipase D based drug delivery strategy of novel phosphatidyl-prodrug被引量：22收藏
- 作者：Tao, Xinyi Jia, Ning Cheng, Nenghui Ren, Yuhong Cao, Xuni Liu, Min Wei, Dongzhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：BIOMATERIALS 2017
- 关键词：Phosphatidyl-prodrug Phospholipase D Doxorubicin Triggered release Reverse multidrug resistance
- Engineering Escherichia coli for l-homoserine production被引量：22收藏
- 作者：Sun, Bing-Yao Wang, Feng-Qing Zhao, Jian Tao, Xin-Yi Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BASIC MICROBIOLOGY 2023
- 关键词：Escherichia coli hok sok system l-homoserine metabolic engineering plasmid stability
- Selective targeting p53WT lung cancer cells harboring homozygous p53 Arg72 by an inhibitor of CypA被引量：21收藏
- 作者：Lu, W. Cheng, F. Yan, W. Li, X. Yao, X. Song, W. Liu, M. Shen, X. Jiang, H. Chen, J. Li, J. Huang, J.
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;Sichuan Univ;Collaborat Innovat Ctr Biotherapy
- 来源：ONCOGENE 2017
- A Self-Sustained System Spanning the Primary and Secondary Metabolism Stages to Boost the Productivity of Streptomyces被引量：19收藏
- 作者：Zhao, Ming Wang, Mingrui Wang, Shuiling Xiong, Liangbin Gao, Bei Liu, Min Tao, Xinyi Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：Streptomyces sigma(hrdB) PkasO* mutant metabolic regulation
- Engineering Saccharomyces cerevisiae for Hyperproduction of β-Amyrin by Mitigating the Inhibition Effect of Squalene on β-Amyrin Synthase被引量：17收藏
- 作者：Du, Meng-Meng Zhu, Zhan-Tao Zhang, Ge-Ge Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Liu, Min Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：beta-amyrin squalene inhibition peroxisome compartmentalization competition pathway Saccharomyces cerevisiae
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae被引量：16收藏
- 作者：Tao, Xin-Yi Lin, Yang-Chen Wang, Feng-Qing Liu, Qing-Hai Ma, Yu-Shu Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2022
- 关键词：Patchoulol Mitochondria compartmentation S cerevisiae Metabolic engineering Sesquiterpene
- RNA-Seq analysis uncovers non-coding small RNA system of Mycobacterium neoaurum in the metabolism of sterols to accumulate steroid intermediates被引量：16收藏
- 作者：Liu, Min Zhu, Zhan-Tao Tao, Xin-Yi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2016
- 关键词：Noncoding RNA Small regulatory RNA RNA sequencing Steroid catabolism Mycobacterium
- Metabolic Adaptation of Mycobacterium neoaurum ATCC 25795 in the Catabolism of Sterols for Producing Important Steroid Intermediates被引量：15收藏
- 作者：Liu, Min Xiong, Liang-Bin Tao, Xinyi Liu, Qing-Hai Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：sterol catabolism mycobacteria cell envelope steroids efflux pumps
- CRISPR/Cpf1 facilitated large fragment deletion in Saccharomyces cerevisiae被引量：15收藏
- 作者：Li, Zhen-Hai Liu, Min Lyu, Xiao-Mei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Nanyang Technol Univ
- 来源：JOURNAL OF BASIC MICROBIOLOGY 2018
- 关键词：CRISPR/Cpf1 large fragment deletion Saccharomyces cerevisiae
- Comparison of the expression of phospholipase D from Streptomyces halstedii in different hosts and its over-expression in Streptomyces lividans被引量：14收藏
- 作者：Tao, Xinyi Zhao, Ming Zhang, Ying Liu, Min Liu, Qinghai Wang, Wei Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：FEMS MICROBIOLOGY LETTERS 2019
- 关键词：phospholipase D Streptomyces lividans Pichia pastoris cytotoxic dual-promoter
- Advances in the structural characterization of complexes of therapeutic antibodies with PD-1 or PD-L1被引量：14收藏
- 作者：Jiang, Mengzhen Liu, Man Liu, Guodi Ma, Jiawen Zhang, Lixin Wang, Shenlin
- 机构：East China Univ Sci & Technol
- 来源：MABS 2023
- 关键词：Antibody structure antibody-antigen interactions PD-1
- CRISPR-Cas12a assisted precise genome editing of Mycolicibacterium neoaurum被引量：13收藏
- 作者：Liu, Ke Gao, Yang Zhen-Hai Li Liu, Min Feng-Qing Wang Dong-Zhi Wei
- 机构：East China Univ Sci & Technol
- 来源：NEW BIOTECHNOLOGY 2022
- 关键词：CRISPR-Cas12a Genome editing Mycolicibacterium neoaurum NHEJ Gene integration
- Immediate, multiplexed and sequential genome engineering facilitated by CRISPR/Cas9 in Saccharomyces cerevisiae被引量：9收藏
- 作者：Li, Zhen-Hai Meng, Hao Ma, Bin Tao, Xinyi Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Hunan Norchem Pharmaceut Co Ltd
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2020
- 关键词：Saccharomyces cerevisiae CRISPR Cas9 Gene targeting Cell factory
- Cpf1-assisted efficient genomic integration of in vivo assembled DNA parts in Saccharomyces cerevisiae被引量：8收藏
- 作者：Li, Zhen-Hai Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2018
- 关键词：CRISPR-Cpf1 Saccharomyces cerevisiae Genomic integration
- CRISPR-Cas Assisted Shotgun Mutagenesis Method for EvolutionaryGenome Engineering被引量：7收藏
- 作者：Zhao, Ming Gao, Miaomiao Xiong, Liangbin Liu, Yongjun Tao, Xinyi Gao, Bei Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Anhui Polytech Univ;Shanghai Univ Med & Hlth Sci
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：CRISPR-mediated genome engineering whole-genome mutagenesis iterative evolution
- Insight into the Structure, Dynamics and the Unfolding Property of Amylosucrases: Implications of Rational Engineering on Thermostability被引量：5收藏
- 作者：Liu, Ming Wang, Shuang Sun, Tingguang Su, Jiguo Zhang, Yuanxing Yue, Junjie Sun, Zhiwei
- 机构：Beijing Inst Biotechnol;E China Univ Sci & Technol;Genor Biopharma Co Ltd;Guangxi Univ Technol;Yanshan Univ
- 来源：PLOS ONE 2012
- Screening of a phage display library of exendin-4 mutants with the extracellular domain of rat GLP-1 receptor被引量：4收藏
- 作者：Yin, Xiaopu Ma, Yushu Liu, Meiyun Gao, Weifeng Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN AND PEPTIDE LETTERS 2007
- 关键词：exendin-4 GLP-1 receptor phage display library
- Exploring the mechanism of a regulatory SNP of KLK3 by molecular dynamics simulation被引量：3收藏
- 作者：Zhang, Zhiyi Liu, Ming Li, Beiping Wang, Yuelan Yue, Junjie Liang, Long Sun, Jianzhong
- 机构：Beijing Inst Biotechnol;Beijing Inst Microbiol & Epidemiol;E China Univ Sci & Technol;Genor Biopharma Co Ltd
- 来源：JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS 2013
- 关键词：KLK3 AR rSNP proteinDNA binding recognition helix molecular dynamics simulation hydrophobic relative motion
- Preparation and purification of novel phosphatidyl prodrug and performance modulation of phosphatidyl nanoprodrug被引量：2收藏
- 作者：Niu, Rui Zhang, PeiLei Wang, Feng-Qing Liu, Min Liu, QingHai Jia, Ning Yang, ShengLi Tao, XinYi Wei, DongZhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2019
- 关键词：Phosphatidyl prodrug Purification Gradient elution Silica gel column chromatography PEGylation RGD modification
