成果/Result
- One-Step, Multiplexed Fluorescence Detection of microRNAs Based on Duplex-Specific Nuclease Signal Amplification被引量：472收藏
- 作者：Yin, Bin-Cheng Liu, Yu-Qiang Ye, Bang-Ce
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 2012
- 关键词：Diagnosis - RNA - Clinical research - Fluorescence - Amplification
- Attomolar Ultrasensitive MicroRNA Detection by DNA-Scaffolded Silver-Nanocluster Probe Based on Isothermal Amplification被引量：231收藏
- 作者：Liu, Yu-Qiang Zhang, Min Yin, Bin-Cheng Ye, Bang-Ce
- 机构：E China Univ Sci & Technol
- 来源：ANALYTICAL CHEMISTRY 2012
- 关键词：Isotherms - Silver - Clinical research - RNA - Cost effectiveness - Diagnosis
- Colorimetric assay for parallel detection of Cd2+, Ni2+ and Co2+ using peptide-modified gold nanoparticles被引量：163收藏
- 作者：Zhang, Min Liu, Yu-Qiang Ye, Bang-Ce
- 机构：E China Univ Sci & Technol
- 来源：ANALYST 2012
- Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol被引量：123收藏
- 作者：Liu, Yiqi Tu, Xiaohu Xu, Qin Bai, Chenxiao Kong, Chuixing Liu, Qi Yu, Jiahui Peng, Qiangqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Methanol Methylotrophic yeast Co-culture Heterologous biosynthesis Monacolin J Lovastatin
- Sensitive Detection of MicroRNA in Complex Biological Samples via Enzymatic Signal Amplification Using DNA Polymerase Coupled with Nicking Endonuclease被引量：77收藏
- 作者：Yin, Bin-Cheng Liu, Yu-Qiang Ye, Bang-Ce
- 机构：E China Univ Sci & Technol
- 来源：ANALYTICAL CHEMISTRY 2013
- 关键词：RNA - Clinical research - Polymerization - Polymerase chain reaction - Diagnosis - Diseases - Isotherms - Signal transduction
- A novel methanol-free Pichia pastoris system for recombinant protein expression被引量：60收藏
- 作者：Shen, Wei Xue, Ying Liu, Yiqi Kong, Chuixing Wang, Xiaolong Huang, Mengmeng Cai, Menghao Zhou, Xiangshan Zhang, Yuanxing Zhou, Mian
- 机构：East China Univ Sci & Technol;SCICB
- 来源：MICROBIAL CELL FACTORIES 2016
- 关键词：Recombination protein expression AOX1 promoter Dihydroxyacetone GUT1 DAK Pichia pastoris
- Multiplexed detection of microRNAs by tuning DNA-scaffolded silver nanoclusters被引量：60收藏
- 作者：Zhang, Min Liu, Yu-Qiang Yu, Cui-Yuan Yin, Bin-Cheng Ye, Bang-Ce
- 机构：E China Univ Sci & Technol
- 来源：ANALYST 2013
- Colorimetric assay for sulfate using positively-charged gold nanoparticles and its application for real-time monitoring of redox process被引量：46收藏
- 作者：Zhang, Min Liu, Yu-Qiang Ye, Bang-Ce
- 机构：E China Univ Sci & Technol
- 来源：ANALYST 2011
- Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast被引量：45收藏
- 作者：Liu, Yiqi Bai, Chenxiao Liu, Qi Xu, Qin Qian, Zhilan Peng, Qiangqiang Yu, Jiahui Xu, Mingqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Ethanol Crabtree-negative yeast Acetyl-CoA Monacolin J Metabolic engineering Coculture
- Rapid and sensitive colorimetric visualization of phthalates using UTP-modified gold nanoparticles cross-linked by copper(II)被引量：41收藏
- 作者：Zhang, Min Liu, Yu-Qiang Ye, Bang-Ce
- 机构：E China Univ Sci & Technol
- 来源：CHEMICAL COMMUNICATIONS 2011
- Expression and Characterization of a Novel Lipase from Aspergillus fumigatus with High Specific Activity被引量：31收藏
- 作者：Shangguan, Jiao-Jiao Liu, Yu-Qiang Wang, Fu-Jun Zhao, Jian Fan, Li-Qiang Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Aspergillus fumigatus Recombinant lipase Expression Characterization Active site
- Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism被引量：30收藏
- 作者：Xu, Qin Bai, Chenxiao Liu, Yiqi Song, Lili Tian, Lin Yan, Yunfeng Zhou, Jinfeng Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY FOR BIOFUELS 2019
- 关键词：Komagataella phaffii Acetate utilization Acetyl-CoA Kinase screening Metabolic engineering
- Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis被引量：26收藏
- 作者：Kong, Chuixing Huang, Hezhou Xue, Ying Liu, Yiqi Peng, Qiangqiang Liu, Qi Xu, Qin Zhu, Qiaoyun Yin, Ying Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SCIENTIFIC REPORTS 2018
- Mononucleotide-Modified Metal Nanoparticles: An Efficient Colorimetric Probe for Selective and Sensitive Detection of Aluminum(III) on Living Cellular Surfaces被引量：25收藏
- 作者：Zhang, Min Liu, Yu-Qiang Ye, Bang-Ce
- 机构：E China Univ Sci & Technol
- 来源：CHEMISTRY-A EUROPEAN JOURNAL 2012
- 关键词：aluminium cell surface colorimetry nanoparticles nucleotides
- Kinase Screening in Pichia pastoris Identified Promising Targets Involved in Cell Growth and Alcohol Oxidase 1 Promoter (PAOX1) Regulation被引量：22收藏
- 作者：Shen, Wei Kong, Chuixing Xue, Ying Liu, Yiqi Cai, Menghao Zhang, Yuanxing Jiang, Tianyi Zhou, Xiangshan Zhou, Mian
- 机构：East China Univ Sci & Technol;SCICB;Roche R&D Ctr China Ltd
- 来源：PLOS ONE 2016
- Characterization of Chitin-Glucan Complex of Ganoderma lucidum Extract and Its Application as Hemostatic Hydrogel被引量：17收藏
- 作者：Liu, Yanqiong Zhang, Chunyue Liu, Lujie Zhang, Xingxing Hou, Yanying Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SC
- 来源：WASTE AND BIOMASS VALORIZATION 2022
- 关键词：Ganoderma lucidum Chitin-glucan complex Ionic liquids Hydrogel Hemostatic material
- Improved methanol-derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast被引量：13收藏
- 作者：Liu, Yiqi Bai, Chenxiao Xu, Qin Yu, Jiahui Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Enhanced biosynthetic pathway Lovastatin Methanol Pichia pastoris Statin pump protein
- Fungal statin pump protein improves monacolin J efflux and regulates its production in Komagataella phaffii被引量：2收藏
- 作者：Bai, Chenxiao Liu, Yiqi Chen, Xinjie Qian, Zhilan Liu, Haifeng Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;China Resources Angde Biotech Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIORESOURCES AND BIOPROCESSING 2020
- 关键词：Statin pump protein Komagataella phaffii Monacolin J Metabolite efflux
- Multiscale CFD-metabolic modeling reveals hybrid impeller configurations mitigate substrate and oxygen starvation in industrial bioreactors被引量：0收藏
- 作者：Gu, Qingfeng Yu, Junxiong Liu, Yongqiang Wang, Yongbo Zhuang, Yingping Li, Chao
- 机构：East China Univ Sci & Technol;Suzhou Womei Biol Co Ltd
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2026
- 关键词：Hybrid impeller configurations Glucose starvation Oxygen starvation Stirred tank reactor CFD modelling Hydrodynamics
- CFD-aided design and hydrodynamic characterization of a single-use perfusion bioreactor for high density cell culture被引量：0收藏
- 作者：Liu, Yongqiang Gu, Qingfeng Liu, Yu Xu, Guoqian Zhuang, Yingping Guo, Meijin Li, Chao
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Alit Biotech Shanghai Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2026
- 关键词：Single-use bioreactor Computational fluid dynamics Culture of animal cells High density culture Shear strain rate Perfusion culture
- Harnessing bioreactor heterogeneity: From gradient understanding to autonomous control via multiscale modeling and intelligent optimization被引量：0收藏
- 作者：Gu, Qingfeng Yu, Junxiong Liu, Yongqiang Wang, Yongbo Li, Chao Zhuang, Yingping
- 机构：East China Univ Sci & Technol;Suzhou Womei Biol Co Ltd
- 来源：BIOTECHNOLOGY ADVANCES 2026
- 关键词：Bioreactor scale-up Multiscale modeling Environmental gradients Digital twin Hybrid modeling Autonomous biomanufacturing
- A sulfated chitosan-driven immune-ECM reprogramming strategy to break the vicious cycle of aged wound healing被引量：0收藏
- 作者：Zhang, Xiaohui Wang, Siyi Liu, Yongqing Wang, Jing Liu, Changsheng
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOACTIVE MATERIALS 2026
- 关键词：Sulfated chitosan Synthetic glycosaminoglycan Macrophage polarization Extracellular matrix degradation Sulfonate groups Aged wound healing
- Multi-objective optimization of oxygen transfer and hydrodynamic shear in dual-impeller stirred tanks via integrated computational fluid dynamics and machine learning framework被引量：0收藏
- 作者：Gu, Qingfeng Ji, Shuoyan Liu, Yongqiang Wang, Yongbo Yu, Junxiong Wang, Zejian Zhuang, Yingping Li, Chao
- 机构：East China Univ Sci & Technol;Suzhou Womei Biol Co Ltd
- 来源：BIORESOURCE TECHNOLOGY 2026
- 关键词：Stirred-tank bioreactor Oxygen mass transfer Shear strain rate Blakesleatrispora beta-carotene
