成果/Result
- A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification被引量：66收藏
- 作者：Luo, Yu Zheng, Yitao Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2006
- High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae被引量：57收藏
- 作者：Liu, Min Lin, Yang-Chen Guo, Jiao-Jiao Du, Meng-Meng Tao, Xinyi Gao, Bei Zhao, Ming Ma, Yushu Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2021
- 关键词：patchoulol physiological status metabolic engineering Saccharomyces cerevisiae sesquiterpene cell factories
- Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp for biodiesel production被引量：45收藏
- 作者：Gao, Bei Su, Erzheng Lin, Jinping Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2009
- 关键词：LipK107 E. coli whole-cell catalyst Biodiesel Low-temperature property
- Mutant library construction in directed molecular evolution被引量：40收藏
- 作者：Wang, Tian-Wen Zhu, Hu Ma, Xing-Yuan Zhang, Ting Ma, Yu-Shu Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2006
- 关键词：directed molecular evolution error-prone PCR oligonucleotide-based mutagenesis DNA shuffling mutator strain somatic hypermutation
- Expression and purification of antimicrobial peptide adenoregulin with C-amidated terminus in Escherichia coli被引量：37收藏
- 作者：Cao, W Zhou, YX Ma, YS Luo, QP Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2005
- 关键词：C-amidated terminus adenoregulin antimicrobial peptide expression Escherichia coli
- Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface被引量：32收藏
- 作者：Jiang, Zhengbing Gao, Bei Ren, Ren Tao, Xingyi Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Hubei Univ
- 来源：BMC BIOTECHNOLOGY 2008
- Metagenomic sequencing reveals microbial gene catalogue of phosphinothricin-utilized soils in South China被引量：29收藏
- 作者：Lou, Jiali Liu, Min Gu, Jiali Liu, Qinghai Zhao, Li Ma, Yushu Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Huzhou Univ
- 来源：GENE 2019
- 关键词：Phosphinothricin N-acetyltransferase Metagenomics Antibiotic resistance genes Virulence factors Microbial community
- N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesei被引量：25收藏
- 作者：Chen, Yumeng Wu, Chuan Shen, Yaling Ma, Yushu Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY FOR BIOFUELS 2019
- 关键词：Trichoderma reesei DMF induced Cellulase Calcium signaling Permeability Phospholipase C plc-e
- High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro被引量：23收藏
- 作者：Ma, Xingyuan Zheng, Wenyun Wei, Dongzhi Ma, Yushu Wang, Tianwen Wang, Jinzhi Liu, Qinghai Yang, Shengli
- 机构：E China Univ Sci & Technol;Shenyang Pharmaceut Univ;Chinese Acad Sci
- 来源：JOURNAL OF BIOTECHNOLOGY 2006
- 关键词：TAT-survivin (T34A) mutant high-level expression and purification pro-apoptosis
- Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans被引量：21收藏
- 作者：Zhang, Lin Lin, Jinping Ma, Yushu Wei, Dongzhi Sun, Ming
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2010
- 关键词：Shuttle vector Gluconobacter oxydans Cryptic plasmid Expression
- A simple approach to mediate genome editing in the filamentous fungus Trichoderma reesei by CRISPR/Cas9-coupled in vivo gRNA transcription被引量：20收藏
- 作者：Wu, Chuan Chen, Yumeng Qiu, Yifei Niu, Xiao Zhu, Ningjian Chen, Jiehui Yao, Hong Wang, Wei Ma, Yushu
- 机构：East China Univ Sci & Technol;East China Normal Univ
- 来源：BIOTECHNOLOGY LETTERS 2020
- 关键词：Filamentous fungus Trichoderma reesei CRISPR Cas9 In vivo gRNA transcription Gene editing
- Expression and purification of exendin-4, a GLP-1 receptor agonist, in Escherichia coli被引量：19收藏
- 作者：Yin, XP Wei, DZ Yi, LN Tao, XY Ma, YS
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2005
- 关键词：exendin-4 expression glucose-lowering action
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae被引量：16收藏
- 作者：Tao, Xin-Yi Lin, Yang-Chen Wang, Feng-Qing Liu, Qing-Hai Ma, Yu-Shu Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2022
- 关键词：Patchoulol Mitochondria compartmentation S cerevisiae Metabolic engineering Sesquiterpene
- An efficient shortened genetic transformation strategy for filamentous fungus Trichoderma reesei被引量：16收藏
- 作者：Wu, Chuan Chen, Yumeng Huang, Xiaoxue Sun, Shishuai Luo, Jinnan Lu, Zhiwen Wang, Wei Ma, Yushu
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 2019
- 关键词：G418 optimized codons Trichoderma reesei two-Agrobacterium-mediated transformation
- Characterisation of a thermostable catechol-2,3-dioxygenase from phenanthrene-degrading Pseudomonas sp strain ZJF08被引量：15收藏
- 作者：Zhou, Ying Wei, Jiashi Jiang, Tao Gao, Weifeng Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Fudan Univ
- 来源：ANNALS OF MICROBIOLOGY 2007
- 关键词：biodegradation catechol-2,3-dioxygenase PAH dioxygenase phenanthrene thermostability
- Characterization of two novel lipase genes isolated directly from environmental sample被引量：13收藏
- 作者：Jiang, Zhengbing Wang, Hongping Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2006
- 关键词：Amino acids - Characterization - Environmental impact - Enzymes - Thermal effects
- In vivo functional expression of an extracellular Ca2+-independent Bacillus pumilus lipase in Bacillus subtilis WB800N被引量：13收藏
- 作者：Ma, Jing Ma, Yushu Wei, Wei Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：ANNALS OF MICROBIOLOGY 2015
- 关键词：Strain screening Lipase Gene cloning Enzymatic properties Bacillus subtilis expression
- Phenanthrene biodegradation by an indigenous Pseudomonas sp ZJF08 with TX100 as surfactant被引量：12收藏
- 作者：Zhou, Ying Zhang, Jiangguo Su, Erzheng Wei, Guodong Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：ANNALS OF MICROBIOLOGY 2008
- 关键词：surfactant phenanthrene biodegradation Pseudomonas sp.
- Display of adenoregulin with a novel Pichia pastoris cell surface display system被引量：12收藏
- 作者：Ren, Ren Jiang, Zhengbing Liu, Meiyun Tao, Xinyi Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2007
- 关键词：adenoregulin cell surface display expression Pichia pastoris
- Cloning and expression of a novel lipase gene from Pseudomonas fluorescens B52被引量：12收藏
- 作者：Jiang, ZB Zheng, YT Luo, Y Wang, G Wang, HP Ma, YS Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2005
- 关键词：expression genome-walking lipase Pichia pastoris Pseudomonas fluorescens
- Structure-based stabilization of an enzyme:: The case of penicillin acylase from Alcaligenes faecalis被引量：12收藏
- 作者：Wang, TW Zhu, H Ma, XY Ma, YS Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN AND PEPTIDE LETTERS 2006
- 关键词：thermal stability penicillin acylase site-directed mutagenesis homology modeling double mutations
- Expression of two old yellow enzyme homologues from Gluconobacter oxydans and identification of their citral hydrogenation abilities被引量：12收藏
- 作者：Yin, Bo Yang, Xuepeng Wei, Guodong Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol;Zhengzhou Univ Light Ind
- 来源：MOLECULAR BIOTECHNOLOGY 2008
- 关键词：Gluconobacter oxydans old yellow enzyme citral NADH FMN-oxidoreductase citronellal
- Application of a silica gel prolonged-release system for methanol in the production of biodiesel被引量：11收藏
- 作者：Luo, Yu Wang, Gang Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 2006
- 关键词：biodiesel lipase prolonged release silica gel
- The transduction of His-TAT-p53 fusion protein into the human osteogenic sarcoma cell line (Saos-2) and its influence on cell cycle arrest and apoptosis被引量：11收藏
- 作者：Jiang, Lei Ma, Yushu Wang, Jinzhi Tao, Xinyi Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：MOLECULAR BIOLOGY REPORTS 2008
- 关键词：apoptosis cell cycle G2 phase transduction
- Antifungal activity of Streptomyces albidoflavus L131 against the leaf mold pathogen Passalora fulva involves membrane leakage and oxidative damage被引量：11收藏
- 作者：Chen, Chao Wang, Yumei Su, Chun Zhao, Xinqing Li, Ming Meng, Xiaowei Jin, Yingyu Yang, Seung-Hwan Ma, Yushu Wei, Dongzhi Suh, Joo-Won
- 机构：Dalian Univ Technol;Dalian Med Univ;Myongji Univ;E China Univ Sci & Technol
- 来源：JOURNAL OF THE KOREAN SOCIETY FOR APPLIED BIOLOGICAL CHEMISTRY 2015
- 关键词：Marine-derived Streptomyces albidoflavus Tomato leaf pathogen Passalorafulva (syn. Fulvia fulva Cladosporium fulvum) Antifungal activity Reactive oxygen species (ROS)
- Production and purification of a novel antibiotic peptide, adenoregulin, from a recombinant Escherichia coli被引量：8收藏
- 作者：Zhou, YX Cao, W Luo, QP Ma, YS Wang, JZ Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2005
- 关键词：adenoregulin Escherichia coli fed-batch culture purification recombinant protein expression
- Characterization of a Novel NADPH-Dependent Oxidoreductase from Gluconobacter oxydans被引量：7收藏
- 作者：Chen, Minmin Lin, Jinping Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2010
- 关键词：Gluconobacter oxydans NADPH-dependent aldehyde reductase GOX0644 Protein expression Substrate specificity
- Comparison of expression of monomeric and multimeric adenoregulin genes in Escherichia coli and Pichia pastorias被引量：7收藏
- 作者：Zhou, YX Cao, W Wang, JZ Ma, YS Wei, DZ
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN AND PEPTIDE LETTERS 2005
- 关键词：antibiotic peptide adenoregulin recombinant expression Escherichia coli Pichia patoris
- Expression and purification of exendin-4 dimer in Escherichia coli and its interaction with GLP-1 receptor in vitro被引量：6收藏
- 作者：Yi, Lina Yin, Xiaopu Wei, Dongzhi Ma, Yushu
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN AND PEPTIDE LETTERS 2006
- 关键词：exendin-4 dimer recombinant expression purification chemical cross-linking
