成果/Result
- HIT: linking herbal active ingredients to targets被引量：252收藏
- 作者：Ye, Hao Ye, Li Kang, Hong Zhang, Duanfeng Tao, Lin Tang, Kailin Liu, Xueping Zhu, Ruixin Liu, Qi Chen, Y. Z. Li, Yixue Cao, Zhiwei
- 机构：Tongji Univ;Shanghai Ctr Bioinformat Technol;E China Univ Sci & Technol;NUS Grad Sch Integrat Sci & Engn;Natl Univ Singapore
- 来源：NUCLEIC ACIDS RESEARCH 2011
- High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells被引量：36收藏
- 作者：Wu, Yixiao Bissinger, Thomas Genzel, Yvonne Liu, Xuping Reichl, Udo Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Max Planck Inst Dynam Complex Tech Syst;Shanghai BioEngine Sci Tech Co Ltd;Otto von Guericke Univ
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2021
- 关键词：MDCK cells Influenza A virus ATF-based perfusion Process optimization Lab-scale bioreactors
- Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines被引量：36收藏
- 作者：Huang, Ding Peng, Wen-Juan Ye, Qian Liu, Xu-Ping Zhao, Liang Fan, Li Xia-Hou, Kang Jia, Han-Jing Luo, Jian Zhou, Lin-Ting Li, Bei-Bei Wang, Shi-Lei Xu, Wen-Ting Chen, Ze Tan, Wen-Song
- 机构：E China Univ Sci & Technol;Shanghai Inst Biol Prod Co Ltd
- 来源：PLOS ONE 2015
- Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production被引量：35收藏
- 作者：Bissinger, Thomas Fritsch, Johannes Mihut, Adrian Wu, Yixiao Liu, Xuping Genzel, Yvonne Tan, Wen-Song Reichl, Udo
- 机构：Max Planck Inst Dynam Complex Tech Syst;East China Univ Sci & Technol;Otto von Guericke Univ
- 来源：VACCINE 2019
- 关键词：MDCK suspension High cell density Influenza A virus production Process intensification Semi-perfusion Cell culture-based vaccine manufacturing
- Identification of multiple sources of the acidic charge variants in an IgG1 monoclonal antibody被引量：35收藏
- 作者：Miao, Shiwei Xie, Panpan Zou, Mao Fan, Li Liu, Xuping Zhou, Yan Zhao, Liang Ding, Ding Wang, Haibin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2017
- 关键词：mAb IgG1 Acidic charge heterogeneity Deamidation Galactosylation Disulfide bond reduction
- Enhancing Immune Response and Heterosubtypic Protection Ability of Inactivated H7N9 Vaccine by Using STING Agonist as a Mucosal Adjuvant被引量：35收藏
- 作者：Luo, Jian Liu, Xu-ping Xiong, Fei-fei Gao, Fei-xia Yi, Ying-lei Zhang, Min Chen, Ze Tan, Wen-song
- 机构：East China Univ Sci & Technol;Shanghai Inst Biol Prod
- 来源：FRONTIERS IN IMMUNOLOGY 2019
- 关键词：H7N9 whole viron vaccine STING mucosal adjuvant cross protection
- Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media: cell growth, antibody production, metabolic waste, glutamate, and energy metabolism被引量：34收藏
- 作者：Zhang, Li-xiang Zhang, Wei-yan Wang, Chen Liu, Jin-tao Deng, Xian-cun Liu, Xu-ping Fan, Li Tan, Wen-song
- 机构： The State Key Laboratory of Bioreactor Engineering; Zhejiang Hisun Pharmaceutical Co. Ltd
- 来源：Bioresources and Bioprocessing 2016
- 关键词：Amino acids - Cell culture - Cell growth - Growth kinetics - Monoclonal antibodies - Physiology
- Elucidating the effects of pH shift on IgG1 monoclonal antibody acidic charge variant levels in Chinese hamster ovary cell cultures被引量：33收藏
- 作者：Xie, Panpan Niu, Huijie Chen, Xinning Zhang, Xintao Miao, Shiwei Deng, Xiancun Liu, Xuping Tan, Wen-Song Zhou, Yan Fan, Li
- 机构：East China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Acidic charge variants IgG1 monoclonal antibody Disulfide bond reduction Galactosylation Deamination Liquid chromatography mass spectrometry
- Galactose supplementation enhance sialylation of recombinant Fc-fusion protein in CHO cell: an insight into the role of galactosylation in sialylation被引量：31收藏
- 作者：Liu, Jintao Wang, Jie Fan, Li Chen, Xinning Hu, Dongdong Deng, Xiancun Poon, H. Fai Wang, Haibin Liu, Xuping Tan, Wen-Song
- 机构：E China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Hangzhou Co Ltd
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2015
- 关键词：Fc-fusion protein CHO cell Glycosylation dSialylation Galactose Galactosylation
- Detailed understanding of enhanced specific productivity in Chinese hamster ovary cells at low culture temperature被引量：26收藏
- 作者：Kou, Tian-Ci Fan, Li Zhou, Yan Ye, Zhao-Yang Liu, Xu-Ping Zhao, Liang Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2011
- 关键词：Chinese hamster ovary (CHO) cells TNFR-Fc mRNA stability Transcriptional rate Low temperature
- Culture temperature modulates monoclonal antibody charge variation distribution in Chinese hamster ovary cell cultures被引量：20收藏
- 作者：Zhang, Xintao Sun, Ya-Ting Tang, Hongping Fan, Li Hu, Dongdong Liu, Jintao Liu, Xuping Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2015
- 关键词：Basic carboxypeptidase Charge variation Chinese hamster ovary cells Culture temperature Lysine variation Monoclonal ntibodies Weak cation exchange chromatography
- Investigation into the impact of tyrosine on the product formation and quality attributes of mAbs in rCHO cell cultures被引量：16收藏
- 作者：Zhang, Weijian Liu, Xuping Tang, Hongping Zhang, Xinran Zhou, Yanan Fan, Li Wang, Haibin Tan, Wen-Song Zhao, Liang
- 机构：East China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2020
- 关键词：Chinese hamster ovary cells Tyrosine Fed-batch cultures Critical quality attribute CD20 Monoclonal antibody
- Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose被引量：15收藏
- 作者：Sun, Ya-ting Zhao, Liang Ye, Zhaoyang Fan, Li Liu, Xu-ping Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2013
- 关键词：CHO cells Fed-batch culture Antibody production Lactate metabolism Galactose Process development
- MOI对HEK293细胞感染病毒后的生长代谢和腺病毒扩增效率的影响被引量：12收藏
- 作者：刘旭平 范里 朱明龙 张旭 谭文松
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《高校化学工程学报》 2010
- 关键词：HEK293细胞 腺病毒 病毒扩增效率 MOI
- 摘要：分别在方瓶贴壁和转瓶悬浮二种培养模式下,考察了感染复数(Multiplicity of Infection,MOI)对HEK293细胞感染病毒后的生长代谢和腺病毒扩增效率的影响。通过实验发现,感染病毒后的细胞跟未感染病毒...
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- Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development被引量：12收藏
- 作者：Liu, Yaya Zhang, Weiyan Deng, Xiancun Poon, Hong Fai Liu, Xuping Tan, Wen-Song Zhou, Yan Fan, Li
- 机构：E China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2015
- 关键词：Basal medium design Chemical-defined Chinese hamster ovary High-yielding Monoclonal antibody
- Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture被引量：11收藏
- 作者：Hu, Dongdong Sun, Yating Liu, Xuping Liu, Jintao Zhang, Xintao Zhao, Liang Wang, Haibin Tan, Wen-Song Fan, Li
- 机构：E China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Chinese hamster ovary cells Yeast extract Fc-fusion protein Specific productivity Cell cycle arrest mTOR
- A novel method based on nonparametric regression with a Gaussian kernel algorithm identifies the critical components in CHO media and feed optimization被引量：11收藏
- 作者：Zou, Mao Zhou, Zi-Wei Fan, Li Zhang, Wei-Jian Zhao, Liang Liu, Xu-Ping Wang, Hai-Bin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Shanghai Bioengine Scitech Co Ltd;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2020
- 关键词：Variable selection Nonparametric regression with Gaussian kernel Chinese hamster ovary cells Medium optimization
- 谷氨酰胺和天冬酰胺对CHO细胞生长、代谢及抗体表达的影响被引量：10收藏
- 作者：张维燕 刘亚亚 刘旭平 范里 谭文松
- 机构：华东理工大学生物反应器国家重点实验室
- 来源：《中国生物工程杂志》 2014
- 关键词：谷氨酰胺 天冬酰胺 比例 总量 抗体 氨 乳酸
- 摘要：以离心换液的批培养为例,通过设计谷氨酰胺和天冬酰胺不同的添加方式来考察两者对CHO细胞生长,代谢及产物表达的影响。结果表明:基础培养基中谷氨酰胺和天冬酰胺不能简单地相互替换,缺失谷氨酰胺或天冬酰胺的基础培养基均不能支持d...
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- Elucidating the effects of arginine and lysine on a monoclonal antibody C-terminal lysine variation in CHO cell cultures被引量：10收藏
- 作者：Zhang, Xintao Tang, Hongping Sun, Ya-Ting Liu, Xuping Tan, Wen-Song Fan, Li
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Chinese hamster ovary cells Monoclonal antibody Lysine variant Arginine Lysine Carboxypeptidase
- Pathway-pathway network-based study of the therapeutic mechanisms by which salvianolic acid B regulates cardiovascular diseases被引量：9收藏
- 作者：Ye Li He Yuan Ye Hao Liu XuePing Yang LinLin Cao ZhiWei Tang KaiLin
- 机构：Tongji Univ;E China Univ Sci & Technol;Shanghai Ctr Bioinformat Technol;Tongji Univ
- 来源：CHINESE SCIENCE BULLETIN 2012
- 关键词：cardiovascular diseases salvianolic acid B network analysis biological pathway network module analysis
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- 微载体浓度与细胞接种密度对ST细胞生长的影响被引量：8收藏
- 作者：陈以衡 陶姝宇 刘旭平 谭文松
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《生物技术通报》 2016
- 关键词：微载体 ST细胞 猪瘟病毒 细胞密度
- 摘要：选择合适的微载体浓度、细胞接种密度以提高微载体利用率,优化微载体培养体系猪睾丸细胞(Swine testicle cells)的贴附生长与维持。使用DMEM补加10%血清、LSM(Low serum medium)两种培...
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- Theoretical study of zeatin - A plant hormone and potential drug for neural diseases - On the basis of DFT, MP2 and target docking被引量：8收藏
- 作者：Liu, Xueping Berezniak, Tomasz Panek, Jaroslaw Jan Jezierska-Mazzarello, Aneta
- 机构：E China Univ Sci & Technol;Univ Wroclaw
- 来源：CHEMICAL PHYSICS LETTERS 2013
- Insights into the generation of monoclonal antibody acidic charge variants during Chinese hamster ovary cell cultures被引量：8收藏
- 作者：Tang, Hongping Miao, Shiwei Zhang, Xintao Fan, Li Liu, Xuping Tan, Wen-Song Zhao, Liang
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2018
- 关键词：Chinese hamster ovary cells Monoclonal antibody Charge heterogeneity Acidic variants Asparagine deamidation
- 金属离子对CHO细胞抗体表达及抗体电荷分布的影响被引量：7收藏
- 作者：张鑫涛 唐红萍 赵亮 范里 刘旭平 缪仕伟 谭文松
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《生物技术通报》 2016
- 关键词：动物细胞培养 电荷分布 金属离子 单克隆抗体
- 摘要：旨在深入认识金属离子在中国仓鼠卵巢(CHO)细胞培养生产单克隆抗体过程中所发挥的作用。综合考察了不同铜离子和锌离子浓度下CHO细胞的生长、抗体的表达以及抗体的电荷分布情况。结果显示,一方面,当培养基中铜离子浓度为120 ...
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- Uridine modulates monoclonal antibody charge heterogeneity in Chinese hamster ovary cell fed-batch cultures被引量：7收藏
- 作者：Niu, Huijie Wang, Jiaqi Liu, Mengjuan Chai, Miaomiao Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Charge heterogeneity Chinese hamster ovary cells Medium components Monoclonal antibody Uridine
- 微载体浓度与细胞接种密度对MDCK细胞生长的影响被引量：7收藏
- 作者：贾涵婧 王逸群 黄锭 罗剑 周琳婷 陈则 刘旭平 谭文松 周燕
- 机构：华东理工大学生物反应器工程国家重点实验室;上海生物制品研究所有限责任公司
- 来源：《中国生物制品学杂志》 2014
- 关键词：肾脏上皮细胞 犬 微载体 细胞密度
- 摘要：目的探讨微载体浓度与犬肾脏上皮（Madin-Darby canine kidney,MDCK）细胞接种密度之间的关系,及其对MDCK细胞生长代谢的影响。方法实验设计了4组不同的单个微载体周围的细胞数范围,分别为0-10、...
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- Highly efficient production of an influenza H9N2 vaccine using MDCK suspension cells被引量：7收藏
- 作者：Wu, Yixiao Jia, Hanjing Lai, Hanzhang Liu, Xuping Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Guangdong Wens Dahuanong Biotechnol Co Ltd;Zhaoqing Dahuanong Biol Med Co Ltd;Shanghai BioEngine Sci Tech Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2020
- 关键词：MDCK suspension cells Cell adaptation Influenza virus Feed strategy Cell culture-based vaccine manufacturing
- 流感病毒生产的维持培养基开发及细胞代谢特征分析被引量：7收藏
- 作者：谢盼 叶倩 刘旭平 谭文松
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《生物技术通报》 2019
- 关键词：流感病毒 维持培养基 MDCK细胞 代谢
- 摘要：采用Simplex Lattice设计开发出适于流感病毒在MDCK细胞中高效扩增的维持培养基,同时以细胞生长培养基作为对照组,研究该培养基下病毒感染后的细胞代谢特征。通过统计学方法,确定维持培养基由A和F培养基以0.58...
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- Characterization and minimization of sialic acid degradation in an Fc-fusion protein-producing CHO cell bioprocess被引量：6收藏
- 作者：Chen, Xinning Liu, Jintao Liu, Xuping Fan, Li Zhao, Liang Tan, Wen-Song
- 机构：East China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2018
- 关键词：Chinese hamster ovary cells Fc-fusion protein Sialic acid Sialidase pH
- Rational design of medium supplementation strategy for improved influenza viruses production based on analyzing nutritional requirements of MDCK Cells被引量：6收藏
- 作者：Huang, Ding Kang Xia-Hou Liu, Xu-Ping Zhao, Liang Fan, Li Ye, Zhaoyang Tan, Wen-Song Luo, Jian Chen, Ze
- 机构：E China Univ Sci & Technol;Shanghai Inst Biol Prod Co Ltd
- 来源：VACCINE 2014
- 关键词：Influenza virus MDCK cells Metabolism Nutritional requirements
