成果/Result
- Inactivation and Augmentation of the Primary 3-Ketosteroid-Δ1-Dehydrogenase in Mycobacterium neoaurum NwIB-01: Biotransformation of Soybean Phytosterols to 4-Androstene-3,17-Dione or 1,4-Androstadiene-3,17-Dione被引量：63收藏
- 作者：Wei, Wei Wang, Feng-qing Fan, Shu-yue Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2010
- A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis被引量：31收藏
- 作者：Wei, Wei Ma, Jing Chen, Si-Qi Cai, Xiang-Hai Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：BMC BIOTECHNOLOGY 2015
- 关键词：Pullulanase type I Paenibacillus polymyxa Cold-adapted pullulanase Functional expression Enzymatic properties Starch hydrolysis products
- Gene cloning and characterization of ferritin H and M subunits from large yellow croaker (Pseudosciaena crocea)被引量：30收藏
- 作者：Zhang, Xiao Wei, Wei Wu, Haizhen Xu, Hongyan Chang, Kangmei Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Zhejiang Ocean Univ
- 来源：FISH & SHELLFISH IMMUNOLOGY 2010
- 关键词：Ferritin Immune-associated Large yellow croaker Attenuated live Vibrio anguillarum
- Accumulation of androstadiene-dione by overexpression of heterologous 3-ketosteroid Δ1-dehydrogenase in Mycobacterium neoaurum NwIB-01被引量：29收藏
- 作者：Wei, Wei Fan, Shu-Yue Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：1,4-Androstadiene-3,17-dione 3-Ketosteroid Delta(1)-dehydrogenase 4-Androstene-3,17-dione Gene overexpression Phytosterols Plasmid stability
- Autotransporter domain-dependent enzymatic analysis of a novel extremely thermostable carboxylesterase with high biodegradability towards pyrethroid pesticides被引量：28收藏
- 作者：Cai, Xianghai Wang, Wei Lin, Lin He, Dannong Huang, Gang Shen, Yaling Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol;Shanghai Univ Med & Hlth Sci
- 来源：SCIENTIFIC REPORTS 2017
- Inactivation and augmentation of the primary 3-Ketosteroid- δ1-Dehydrogenase in mycobacterium neoaurum NwIB-01: Biotransformation of soybean phytosterols to 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione被引量：27收藏
- 作者：Wei, Wei Wang, Feng-Qing Fan, Shu-Yue Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2010
- 关键词：Gene encoding - Polyols
- A type I pullulanase of Bacillus cereus Nws-bc5 screening from stinky tofu brine: Functional expression in Escherichia coli and Bacillus subtilis and enzyme characterization被引量：26收藏
- 作者：Wei, Wei Ma, Jing Guo, Su Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2014
- 关键词：Stinky tofu brine Strain screening Pullulanase Gene cloning Enzymatic properties Bacillus subtilis expression
- Functional expression of a novel alkaline-adapted lipase of Bacillus amyloliquefaciens from stinky tofu brine and development of immobilized enzyme for biodiesel production被引量：26收藏
- 作者：Cai, Xianghai Ma, Jing Wei, Dong-zhi Lin, Jin-ping Wei, Wei
- 机构：E China Univ Sci & Technol
- 来源：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 2014
- 关键词：Bacillus amyloliquefaciens Lipase Gene cloning Functional expression Immobilization
- N-Terminal Domain Truncation and Domain Insertion-Based Engineering of a Novel Thermostable Type I Pullulanase from Geobacillus thermocatenulatus被引量：23收藏
- 作者：Li, Lingmeng Dong, Fengying Lin, Lin He, Dannong Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：Geobacillus thermocatenulatus pullulanase domain truncation and insertion maize starch
- A New Steroid-Transforming Strain of Mycobacterium neoaurum and Cloning of 3-Ketosteroid 9α-Hydroxylase in NwIB-01被引量：23收藏
- 作者：Wei, Wei Fan, Shuyue Wang, Fengqing Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Mycobacterium neoaurum Extraction chromatography Screening Phytosterols 4-Androstene-3,17-dione (AD) 1,4-Androstadiene-3,17-dione (ADD) 3-Ketosteroid 9 alpha-hydroxylase (KSH)
- Identification of differentially expressed genes in large yellow croaker (Pseudosciaena crocea) induced by attenuated live Vibrio anguillarum被引量：17收藏
- 作者：Wei, Wei Xu, Hongyan Wang, Qiyao Zhang, Xiao Chang, Kangmei Wu, Changwen Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Zhejiang Ocean Univ
- 来源：AQUACULTURE 2009
- 关键词：Differentially expressed gene Immune-relevant gene Large yellow croaker Suppression subtractive hybridization (SSH) Vibrio anguillarum
- Biochemical Characterization of a Novel Thermostable Type I Pullulanase Produced Recombinantly in Bacillus subtilis被引量：16收藏
- 作者：Li, Lingmeng Dong, Fengying Lin, Lin He, Dannong Chen, Jingwen Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol;Univ South Carolina
- 来源：STARCH-STARKE 2018
- 关键词：Bacillus subtilis enzymatic properties expression Geobacillus kaustophilus pullulanase thermostable secretion expression
- Cloning and molecular characterization of two complement Bf/C2 genes in large yellow croaker (Pseudosciaena crocea)被引量：16收藏
- 作者：Wei, Wei Wu, Haizhen Xu, Hongyan Xu, Tao Zhang, Xiao Chang, Kangmei Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Zhejiang Ocean Univ
- 来源：FISH & SHELLFISH IMMUNOLOGY 2009
- 关键词：Complement factor B Complement C2 Large yellow croaker 3D structure Attenuated live V. anguillarum
- Construction of a Shuttle Vector for Protein Secretory Expression in Bacillus subtilis and the Application of the Mannanase Functional Heterologous Expression被引量：16收藏
- 作者：Guo, Su Tang, Jia-jie Wei, Dong-zhi Wei, Wei
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：Bacillus subtilis Bacillus pumilus green fluorescent protein mannanase P43 promoter secretory expression
- A light-switchable bidirectional expression system in filamentous fungus Trichoderma reesei被引量：15收藏
- 作者：Zhang, Guoxiu Liu, Pei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Trichoderma reesei Light-switchable system Heterologous protein Simultaneous expression Cellulase-free background
- Enhancement of ethyl (S)-4-chloro-3-hydroxybutanoate production at high substrate concentration by in situ resin adsorption被引量：14收藏
- 作者：Chen, Li-Feng Fan, Hai-Yang Zhang, Yi-Ping Wei, Wei Lin, Jin-Ping Wei, Dong-Zhi Wang, Hua-Lei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Ethyl (S)-4-chloro-3-hydroxybutanoate Carbonyl reductase Asymmetric reduction Product inhibition Resin adsorption
- Protopectinase production by Paenibacillus polymyxa Z6 and its application in pectin extraction from apple pomace被引量：14收藏
- 作者：Zhang, Jian Zhao, Li Gao, Bei Wei, Wei Wang, Hualei Xie, Jingli
- 机构：East China Univ Sci & Technol;SCICB
- 来源：JOURNAL OF FOOD PROCESSING AND PRESERVATION 2018
- 关键词：Fruits - Moisture - Energy utilization - Fermentation - Viscosity
- Synergistic Regulation of Metabolism by Ca2+/Reactive Oxygen Species in Penicillium brevicompactum Improves Production of Mycophenolic Acid and Investigation of the Ca2+ Channel被引量：14收藏
- 作者：Chen, Mianhui Wang, Jingjing Lin, Lin Xu, Xiangyang Wei, Wei Shen, Yaling Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol;Zaozhuang Jie Nuo Enzyme Co Ltd
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：mycophenolic acid (MPA) ROS calcium pigment Penicillium brevicompactum VHb
- Cinnamyl Esters Synthesis By Lipase-Catalyzed Transesterification in a Non-Aqueous System被引量：13收藏
- 作者：Cai, Xianghai Wang, Wei Lin, Lin He, Dannong Shen, Yaling Wei, Wei Wei, Dong-zhi
- 机构：East China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol;Shanghai Univ Med & Hlth Sci
- 来源：CATALYSIS LETTERS 2017
- 关键词：Enzymatic catalysis Cinnamyl esters Green chemistry
- In vivo functional expression of an extracellular Ca2+-independent Bacillus pumilus lipase in Bacillus subtilis WB800N被引量：13收藏
- 作者：Ma, Jing Ma, Yushu Wei, Wei Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：ANNALS OF MICROBIOLOGY 2015
- 关键词：Strain screening Lipase Gene cloning Enzymatic properties Bacillus subtilis expression
- Cloning and molecular characterization of lycC1INH genes in large yellow croaker (Pseudosciaena crocea)被引量：12收藏
- 作者：Wei, Wei Wu, Haizhen Xu, Hongyan Zhang, Xiao Chu, Teng Wu, Changwen Chang, Kangmei Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Zhejiang Ocean Univ
- 来源：FISH & SHELLFISH IMMUNOLOGY 2010
- 关键词：Large yellow croaker (Pseudosciana crocea) Vibrio anguillarum lycC1INH Immune response
- Enhanced Thermostability of Geobacillus stearothermophilus α-Amylase by Rational Design of Disulfide Bond and Application in Corn Starch Liquefaction and Bread Quality Improvement被引量：11收藏
- 作者：Zhu, Mengyu Zhai, Wenxin Song, Runfei Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2023
- 关键词：alpha-amylase disulfide bond thermostability force network corn starch liquefaction breadaging
- Low-Temperature Synthesized Well-Crystallized ZnO Microspheres for Photocatalysis被引量：11收藏
- 作者：Wei, Wei Lin, Lin Cai, Xianghai Li, Yiwen Yu, Zhiqiang Zhong, Jian
- 机构：E China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol;Shanghai Ocean Univ;Univ Calif San Diego;Southern Med Univ
- 来源：SCIENCE OF ADVANCED MATERIALS 2016
- 关键词：Photocatalysis ZnO Flower-Like Microsphere Nanocrystal
- Functional expression of a novel methanol-stable esterase from Geobacillus subterraneusDSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system被引量：10收藏
- 作者：Cai, Xianghai Lin, Lin Shen, Yaling Wei, Wei Wei, Dong-zhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol
- 来源：BMC BIOTECHNOLOGY 2020
- 关键词：Geobacillus subterraneus Esterase Methanol-stable Biocatalysis Cinnamyl acetate
- Structural and biochemical properties of a novel pullulanase of Paenibacillus lautus DSM 3035被引量：10收藏
- 作者：Chen, Si-Qi Cai, Xiang-Hai Xie, Jing-Li Wei, Wei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：STARCH-STARKE 2017
- 关键词：Enzymatic properties Functional expression Hydrolysis products Paenibacillus lautus Pullulanase type I
- A novel host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei industrial strain RUT-C30被引量：9收藏
- 作者：Zhang, Lei Zhang, Suping Jiang, Xin Wei, Wei Wang, Wei Wei, Dongzhi
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;Tianjin Hualida Biotechnol Co Ltd
- 来源：BIOTECHNOLOGY LETTERS 2016
- 关键词：Alkaline cellulose Co-expression Filamentous fungi Phosphinothricin xyn2 Promoter Trichoderma reesei
- Transesterification Synthesis of Chloramphenicol Esters with the Lipase from Bacillus amyloliquefaciens被引量：9收藏
- 作者：Dong, Fengying Li, Lingmeng Lin, Lin He, Dannong Chen, Jingwen Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol;Univ South Carolina
- 来源：MOLECULES 2017
- 关键词：enzymatic catalysis regioselectivity chloramphenicol esters green chemistry Bacillus amyloliquefaciens
- Mn2+ modulates the production of mycophenolic acid in Penicillium brevicompactum NRRL864 via reactive oxygen species signaling and the investigation of pb-pho被引量：8收藏
- 作者：Chen, Mianhui Shen, Yaling Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：FUNGAL BIOLOGY 2022
- 关键词：Manganese-calcium-ROS Penicillium brevicompactum Cell wall integrity Mycophenolic acid Heat shock
- Bioprospecting metagenomics of a microbial community on cotton degradation: Mining for new glycoside hydrolases被引量：8收藏
- 作者：Zhang, Guoxiu Liu, Pei Zhang, Lei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Glycoside hydrolase Microbial community Metagenomics Crude cotton Expression
- Exploiting loop-grafting strategy resorting on computer-aided design to improve the thermostability of alpha-amylase from Geobacillus stearothermophilus被引量：8收藏
- 作者：Zhu, Mengyu Zhai, Wenxin Jiang, Haolin Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Nanjing Univ Informat Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：PROCESS BIOCHEMISTRY 2023
- 关键词：Alpha-amylase Loop-grafting Molecular dynamic simulation Thermostability Corn starch liquefaction
