成果/Result
- Switching Cofactor Dependence of 7β-Hydroxysteroid Dehydrogenase for Cost-Effective Production of Ursodeoxycholic Acid被引量：66收藏
- 作者：You, Zhi-Neng Chen, Qi Shi, Shou-Cheng Zheng, Ming-Min Pan, Jiang Qian, Xiao-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis cofactor dependence small-and-smart library 7 beta-hydroxysteroid dehydrogenase molecular dynamics simulation ursodeoxycholic acid
- Stereoselective synthesis of L-tert-leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum被引量：58收藏
- 作者：Li, Jing Pan, Jiang Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2014
- 关键词：Leucine dehydrogenase Exiguobacterium sibiricum Reductive amination Dual enzyme co-expression L-tert-Leucine
- A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: Catalytic properties and performance in kinetic resolution of α-hydroxy acids被引量：49收藏
- 作者：Ma, Bao-Di Yu, Hui-Lei Pan, Jiang Liu, Jia-Yan Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2013
- 关键词：Esterase Thermostability Organic-solvent tolerance alpha-Hydroxy (carboxylic) acids Deacetylation
- Rational Engineering of Formate Dehydrogenase Substrate/Cofactor Affinity for Better Performance in NADPH Regeneration被引量：46收藏
- 作者：Jiang, He-Wen Chen, Qi Pan, Jiang Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2020
- 关键词：Binding affinity Burkholderia stabilis Formate dehydrogenase NADPH regeneration Rational engineering
- Bioproduction of chiral mandelate by enantioselective deacylation of α-acetoxyphenylacetic acid using whole cells of newly isolated Pseudomonas sp ECU1011被引量：38收藏
- 作者：Ju, Xin Yu, Hui-Lei Pan, Jiang Wei, Dong-Zhi Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2010
- 关键词：Microbial esterase Pseudomonas sp. Enantioselective deacylation alpha-Acetoxyphenylacetic acid Enantiopure mandelic acid
- Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010被引量：38收藏
- 作者：Zhao, Li-Li Xu, Jian-He Zhao, Jian Pan, Jiang Wang, Zhi-Long
- 机构： Laboratory of Biocatalysis and Bioprocessing; School of Pharmacy
- 来源：Process Biochemistry 2008
- 关键词：Bacteria - Chirality - Dimers - Enantiomers - Enzyme activity - Organic solvents
- Efficient production of diltiazem chiral intermediate using immobilized lipase from serratia marcescens被引量：34收藏
- 作者：Zhao, Li-Li Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Biotechnology and Bioprocess Engineering 2010
- 关键词：Enzyme activity - Ethers - Enzyme immobilization - Tanks (containers)
- Enzymatic synthesis of high-titer nicotinamide mononucleotide with a new nicotinamide riboside kinase and an efficient ATP regeneration system被引量：34收藏
- 作者：Qian, Xiao-Long Dai, Yi-Si Li, Chun-Xiu Pan, Jiang Xu, Jian-He Mu, Bozhong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Enzymatic phosphorylation Nicotinamide riboside Nicotinamide riboside kinase beta-Nicotinamide mononucleotide (NMN) ATP regeneration system
- Immobilization of glycolate oxidase from Medicago falcata on magnetic nanoparticles for application in biosynthesis of glyoxylic acid被引量：34收藏
- 作者：Zhu, Hong Pan, Jiang Hu, Bin Yu, Hui-Lei Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2009
- 关键词：Nanomagnetics - Nanoparticles - Fourier transform infrared spectroscopy - Batch reactors - Enzyme immobilization - Enzyme activity - Synthesis (chemical) - Biochemistry - Plants (botany) - Scanning electron microscopy
- Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system被引量：33收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Liu, Jun-Feng Xu, Jian-He He, Yu-Cai Liu, You-Yan
- 机构： Laboratory of Biocatalysis and Bioprocessing; College of Chemistry and Chemical Engineering; College of Chemistry and Chemical Engineering
- 来源：Journal of Biotechnology 2011
- 关键词：Cells - Cytology - Enantioselectivity - Escherichia coli - Hydrolysis - Organic acids - Organic solvents - Productivity - Toluene
- Efficient production of l-menthol in a two-phase system with SDS using an immobilized bacillus subtilis esterase被引量：32收藏
- 作者：Pan, Jiang Dang, Ngoc-Duy Zheng, Gao-Wei Cheng, Bo Ye, Qin Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; Sunflower Pharmaceutical Group Company Limited
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Cloning - Crosslinking - Enantioselectivity - Enzymatic hydrolysis - Enzyme activity - Enzyme inhibition - Esters - Sulfur compounds
- Resolution of racemic sulfoxides with high productivity and enantioselectivity by a Rhodococcus sp. strain as an alternative to biooxidation of prochiral sulfides for efficient production of enantiopure sulfoxides被引量：32收藏
- 作者：Li, Ai-Tao Yu, Hui-Lei Pan, Jiang Zhang, Jian-Dong Xu, Jian-He Lin, Guo-Qiang
- 机构： Laboratory of Biocatalysis and Bioprocessing; Shanghai Institute of Organic Chemistry
- 来源：Bioresource Technology 2011
- 关键词：Substrates - Enantioselectivity
- Isolation and properties of a levo-lactonase from Fusarium proliferatum ECU2002: A robust biocatalyst for production of chiral lactones被引量：31收藏
- 作者：Zhang, Xian Xu, Jian-He Xu, Yi Pan, Jiang
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Applied Microbiology and Biotechnology 2007
- 关键词：Aldehydes - Bacteria - Concentration (process) - Electrophoresis - Ketones
- Crosslinking of enzyme coaggregate with polyethylenEImine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase被引量：31收藏
- 作者：Pan, Jiang Kong, Xu-Dong Li, Chun-Xiu Ye, Qin Xu, Jian-He Imanaka, Tadayuki
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biotechnology
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Coprecipitation - Enzyme immobilization
- Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of dl-menthyl acetate被引量：31收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Li, Chun-Xiu Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Bacteriology - Monoterpenes - Nitrogen compounds - Cloning - Esters - Enzyme immobilization
- Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system被引量：30收藏
- 作者：Li, Ai-Tao Zhang, Jian-Dong Yu, Hui-Lei Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Process Biochemistry 2011
- 关键词：Oxidation - Enantiomers - Paraffins - Alcohols - Polyols - Solvent extraction
- Efficient synthesis of a statin precursor in high space-time yield by a new aldehyde-tolerant aldolase identified from Lactobacillus brevis被引量：30收藏
- 作者：Jiao, Xue-Cheng Pan, Jiang Xu, Guo-Chao Kong, Xu-Dong Chen, Qi Zhang, Zhi-Jun Xu, Jian-He
- 机构：E China Univ Sci & Technol; E China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2015
- 关键词：Crystal structure - Stereochemistry - Acetaldehyde - Hydrogen bonds - Ketones - Stability - Substrates
- Improving Pseudomonas sp. esterase performance by engineering approaches for kinetic resolution of 2-acetoxyphenylacetic acids被引量：29收藏
- 作者：Ju, Xin Pan, Jiang Yu, Hui-Lei Li, Chun-Xiu Xu, Jian-He
- 机构： Lab. of Biocatalysis and Synthetic Biotechnology
- 来源：Biochemical Engineering Journal 2011
- 关键词：Bacteria - Cells - Enzymes - Organic acids - Hydrolysis - Molecular biology - Cell culture - Esters
- Overexpression of Serratia marcescens lipase in Escherichia coli for efficient bioresolution of racemic ketoprofen被引量：29收藏
- 作者：Long, Zhang-De Xu, Jian-He Zhao, Li-Li Pan, Jiang Yang, Sheng Hua, Ling
- 机构： Laboratory of Biocatalysis and Bioprocessing; Agricultural College; Institute of Plant Physiology and Ecology; Department of Chemistry
- 来源：Journal of Molecular Catalysis B: Enzymatic 2007
- 关键词：Biocatalysts - Enantioselectivity - Esters - Lipases - Optimization - Substrates - Surface active agents
- Efficient reduction of aromatic ketones with NADPH regeneration by using crude enzyme from Rhodotorula cells and mannitol as cosubstrate被引量：28收藏
- 作者：Yang, Wei Xu, Jian-He Pan, Jiang Xu, Yi Wang, Zhi-Long
- 机构： Laboratory of Biocatalysis and Bioprocessing; School of Pharmacy
- 来源：Biochemical Engineering Journal 2008
- 关键词：Enzymes - Polyols - Aromatic compounds - Drug products
- Immobilization of Serratia marcescens lipase onto amino-functionalized magnetic nanoparticles for repeated use in enzymatic synthesis of Diltiazem intermediate被引量：27收藏
- 作者：Hu, Bin Pan, Jiang Yu, Hui-Lei Liu, Jian-Wen Xu, Jian-He
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2009
- 关键词：Nanoparticles - ProtEIns - High resolution transmission electron microscopy - Reaction intermediates - Fourier transform infrared spectroscopy - Magnetite - Coprecipitation - Nanomagnetics - Synthesis (chemical)
- Marked enhancement of Acinetobacter sp. Organophosphorus hydrolase activity by a single residue substitution Ile211Ala被引量：27收藏
- 作者：Chen, Jie Luo, Xiao-Jing Chen, Qi Pan, Jiang Zhou, Jiahai Xu, Jian-He
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai Institute of Organic Chemistry
- 来源：Bioresources and Bioprocessing 2015
- 关键词：Hydrolases
- Efficient production of (S)-naproxen with (R)-substrate recycling using an overexpressed carboxylesterase BsE-NP01被引量：27收藏
- 作者：Liu, Xiang Xu, Jian-He Pan, Jiang Zhao, Jing
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Applied Biochemistry and Biotechnology 2010
- 关键词：Escherichia coli - Hydrolysis - Bacteriology - Enantiomers - Enzymes - Cloning - Enantioselectivity - Esters
- Preparation of (R)-(-)-mandelic acid and its derivatives from racemates by enantioselective degradation with a newly isolated bacterial strain Alcaligenes sp. ECU0401被引量：27收藏
- 作者：He, Yu-Cai Xu, Jian-He Pan, Jiang Ouyang, Li-Ming Xu, Yi
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Bioprocess and Biosystems Engineering 2008
- 关键词：Organic acids - Enantiomers - Batch cell culture
- Remodeling the Homologous Recombination Mechanism of Yarrowia lipolytica for High-Level Biosynthesis of Squalene被引量：26收藏
- 作者：Xu, Man Yang, Nan Pan, Jiang Hua, Qiang Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2024
- 关键词：homologous recombination squalene Yarrowia lipolytica metabolic engineering acetyl coenzyme A
- An efficient bioprocess for enzymatic production of l-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli被引量：26收藏
- 作者：Zheng, Gao-Wei Pan, Jiang Yu, Hui-Lei Ngo-Thi, Minh-Thu Li, Chun-Xiu Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Biotechnology 2010
- 关键词：Bacteriology - Cloning - Escherichia coli - Monoterpenes - Esters - Cytology - Gene encoding - Catalysis - Enzymes - Loading
- Efficient Stereoselective Synthesis of Structurally Diverse γ- and δ-Lactones Using an Engineered Carbonyl Reductase被引量：26收藏
- 作者：Chen, Meng Zhang, Xiao-Yan Xing, Chen-Guang Zhang, Chao Zheng, Yu-Cong Pan, Jiang Xu, Jian-He Bai, Yun-Peng
- 机构：East China Univ Sci & Technol;Xiamen Oamic Biotech Co Ltd
- 来源：CHEMCATCHEM 2019
- 关键词：Asymmetric reduction directed evolution chiral lactones carbonyl reductase biocatalysis
- Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp nitrilase被引量：24收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2014
- 关键词：Immobilized cells Alcaligenes sp nitrilase Glutaraldehyde cross-linking Enantioselective hydrolysis (R)-(-)-Mandelic acid
- Optimization and Scale-up of a Bioreduction Process for Preparation of Ethyl (S)-4-Chloro-3-hydroxybutanoate被引量：23收藏
- 作者：Pan, Jiang Zheng, Gao-Wei Ye, Qin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2014
- 关键词：Coenzymes
- Significantly enhanced production of recombinant nitrilase by optimization of culture conditions and glycerol feeding被引量：21收藏
- 作者：Liu, Jun-Feng Zhang, Zhi-Jun Li, Ai-Tao Pan, Jiang Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：Nitrilase Optimization Fermentation Glycerol feeding Recombinant Escherichia coli
