成果/Result
- A novel cold-adapted type I pullulanase of Paenibacillus polymyxa Nws-pp2: in vivo functional expression and biochemical characterization of glucans hydrolyzates analysis被引量：31收藏
- 作者：Wei, Wei Ma, Jing Chen, Si-Qi Cai, Xiang-Hai Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：BMC BIOTECHNOLOGY 2015
- 关键词：Pullulanase type I Paenibacillus polymyxa Cold-adapted pullulanase Functional expression Enzymatic properties Starch hydrolysis products
- Accumulation of androstadiene-dione by overexpression of heterologous 3-ketosteroid Δ1-dehydrogenase in Mycobacterium neoaurum NwIB-01被引量：29收藏
- 作者：Wei, Wei Fan, Shu-Yue Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：1,4-Androstadiene-3,17-dione 3-Ketosteroid Delta(1)-dehydrogenase 4-Androstene-3,17-dione Gene overexpression Phytosterols Plasmid stability
- Inactivation and augmentation of the primary 3-Ketosteroid- δ1-Dehydrogenase in mycobacterium neoaurum NwIB-01: Biotransformation of soybean phytosterols to 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione被引量：27收藏
- 作者：Wei, Wei Wang, Feng-Qing Fan, Shu-Yue Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2010
- 关键词：Gene encoding - Polyols
- A type I pullulanase of Bacillus cereus Nws-bc5 screening from stinky tofu brine: Functional expression in Escherichia coli and Bacillus subtilis and enzyme characterization被引量：26收藏
- 作者：Wei, Wei Ma, Jing Guo, Su Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2014
- 关键词：Stinky tofu brine Strain screening Pullulanase Gene cloning Enzymatic properties Bacillus subtilis expression
- N-Terminal Domain Truncation and Domain Insertion-Based Engineering of a Novel Thermostable Type I Pullulanase from Geobacillus thermocatenulatus被引量：23收藏
- 作者：Li, Lingmeng Dong, Fengying Lin, Lin He, Dannong Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：Geobacillus thermocatenulatus pullulanase domain truncation and insertion maize starch
- A New Steroid-Transforming Strain of Mycobacterium neoaurum and Cloning of 3-Ketosteroid 9α-Hydroxylase in NwIB-01被引量：23收藏
- 作者：Wei, Wei Fan, Shuyue Wang, Fengqing Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Mycobacterium neoaurum Extraction chromatography Screening Phytosterols 4-Androstene-3,17-dione (AD) 1,4-Androstadiene-3,17-dione (ADD) 3-Ketosteroid 9 alpha-hydroxylase (KSH)
- Biochemical Characterization of a Novel Thermostable Type I Pullulanase Produced Recombinantly in Bacillus subtilis被引量：16收藏
- 作者：Li, Lingmeng Dong, Fengying Lin, Lin He, Dannong Chen, Jingwen Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol;Univ South Carolina
- 来源：STARCH-STARKE 2018
- 关键词：Bacillus subtilis enzymatic properties expression Geobacillus kaustophilus pullulanase thermostable secretion expression
- A light-switchable bidirectional expression system in filamentous fungus Trichoderma reesEI被引量：15收藏
- 作者：Zhang, Guoxiu Liu, Pei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Trichoderma reesEI Light-switchable system Heterologous protEIn Simultaneous expression Cellulase-free background
- Enhancement of ethyl (S)-4-chloro-3-hydroxybutanoate production at high substrate concentration by in situ resin adsorption被引量：14收藏
- 作者：Chen, Li-Feng Fan, Hai-Yang Zhang, Yi-Ping Wei, Wei Lin, Jin-Ping Wei, Dong-Zhi Wang, Hua-Lei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Ethyl (S)-4-chloro-3-hydroxybutanoate Carbonyl reductase Asymmetric reduction Product inhibition Resin adsorption
- Protopectinase production by Paenibacillus polymyxa Z6 and its application in pectin extraction from apple pomace被引量：14收藏
- 作者：Zhang, Jian Zhao, Li Gao, Bei Wei, Wei Wang, Hualei Xie, Jingli
- 机构：East China Univ Sci & Technol;SCICB
- 来源：JOURNAL OF FOOD PROCESSING AND PRESERVATION 2018
- 关键词：Fruits - Moisture - Energy utilization - Fermentation - Viscosity
- Cinnamyl Esters Synthesis By Lipase-Catalyzed Transesterification in a Non-Aqueous System被引量：13收藏
- 作者：Cai, Xianghai Wang, Wei Lin, Lin He, Dannong Shen, Yaling Wei, Wei Wei, Dong-zhi
- 机构：East China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol;Shanghai Univ Med & Hlth Sci
- 来源：CATALYSIS LETTERS 2017
- 关键词：Enzymatic catalysis Cinnamyl esters Green chemistry
- Enhanced Thermostability of Geobacillus stearothermophilus α-Amylase by Rational Design of Disulfide Bond and Application in Corn Starch Liquefaction and Bread Quality Improvement被引量：11收藏
- 作者：Zhu, Mengyu Zhai, Wenxin Song, Runfei Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2023
- 关键词：alpha-amylase disulfide bond thermostability force network corn starch liquefaction breadaging
- Functional expression of a novel methanol-stable esterase from Geobacillus subterraneusDSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system被引量：10收藏
- 作者：Cai, Xianghai Lin, Lin Shen, Yaling Wei, Wei Wei, Dong-zhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol
- 来源：BMC BIOTECHNOLOGY 2020
- 关键词：Geobacillus subterraneus Esterase Methanol-stable Biocatalysis Cinnamyl acetate
- Structural and biochemical properties of a novel pullulanase of Paenibacillus lautus DSM 3035被引量：10收藏
- 作者：Chen, Si-Qi Cai, Xiang-Hai Xie, Jing-Li Wei, Wei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：STARCH-STARKE 2017
- 关键词：Enzymatic properties Functional expression Hydrolysis products Paenibacillus lautus Pullulanase type I
- A novel host-vector system for heterologous protEIn co-expression and purification in the Trichoderma reesEI industrial strain RUT-C30被引量：9收藏
- 作者：Zhang, Lei Zhang, Suping Jiang, Xin Wei, Wei Wang, Wei Wei, Dongzhi
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;Tianjin Hualida Biotechnol Co Ltd
- 来源：BIOTECHNOLOGY LETTERS 2016
- 关键词：Alkaline cellulose Co-expression Filamentous fungi Phosphinothricin xyn2 Promoter Trichoderma reesEI
- Bioprospecting metagenomics of a microbial community on cotton degradation: Mining for new glycoside hydrolases被引量：8收藏
- 作者：Zhang, Guoxiu Liu, Pei Zhang, Lei Wei, Wei Wang, Xuedong Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Glycoside hydrolase Microbial community Metagenomics Crude cotton Expression
- Exploiting loop-grafting strategy resorting on computer-aided design to improve the thermostability of alpha-amylase from Geobacillus stearothermophilus被引量：8收藏
- 作者：Zhu, Mengyu Zhai, Wenxin Jiang, Haolin Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Nanjing Univ Informat Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：PROCESS BIOCHEMISTRY 2023
- 关键词：Alpha-amylase Loop-grafting Molecular dynamic simulation Thermostability Corn starch liquefaction
- An Acid-Adapted Endo-α-1,5-L-arabinanase for Pectin Releasing被引量：7收藏
- 作者：Lang, Chong Yang, Rujian Yang, Ying Gao, Bei Zhao, Li Wei, Wei Wang, Hualei Matsukawa, Shingo Xie, Jingli Wei, Dongzhi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Tokyo Univ Marine Sci & Technol;SCICB
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2016
- 关键词：Endo-1,5-alpha-L-arabinanase GH43 Acid-adapted Pectin extraction Apple pomace
- Identification, Characterization, and Computer-Aided Rational Design of a Novel Thermophilic Esterase from Geobacillus subterraneus, and Application in the Synthesis of Cinnamyl Acetate被引量：6收藏
- 作者：Zhang, Jin Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2024
- 关键词：Thermophilic esterase Rational design Thermal stability ADAP strategy Cinnamyl acetate
- Esterase-Immobilized Sea-Urchin-Like Fe3O4 Nanoparticles for Chloramphenicol Palmitate Synthesis被引量：5收藏
- 作者：Dong, Fengying Lin, Lin Su, Yue Zhang, Chuan Wei, Wei
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Shanghai Inst Technol
- 来源：CATALYSIS LETTERS 2023
- 关键词：Polydopamine-coated Fe3O4 nanoparticle Dialdehyde starch Esterase Compartmentation immobilization Regio-selectivity Chloramphenicol palmitate
- High-level expression of a β-mannanase (manB) in Pichia pastoris GS115 for mannose production with Penicillium brevicompactum fermentation pretreatment of soybean meal被引量：5收藏
- 作者：Chen, Mianhui Wang, Jingjing Lin, Lin Wei, Wei Shen, Yaling Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2021
- 关键词：beta-Mannanase Soybean meal Bio-fermentation pretreated Mannose Penicillium brevicompactum
- Pyomelanin production via heterologous expression of 4-hydroxyphenylpyruvate dioxygenase (HPPD) and construction of HPPD inhibitor screening model被引量：5收藏
- 作者：Zhang, Qihao Yang, Xiaohui Lin, Lin Wu, Shuhong Wang, Ping Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol;Shanghai Jiao Tong Univ;Weigao Shanghai R&D Ctr
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2023
- 关键词： Pyomelanin production 4-Hydroxyphenylpyruvate dioxygenase 4-Hydroxyphenylpyruvate dioxygenase inhibitor Screening model UV protection]
- A Type I Pullulanase from Geobacillus subterraneus: Functional Expression in Escherichia coli, Enzyme Characterization, Truncation, and Application被引量：5收藏
- 作者：Chen, Mianhui Zhang, Jin Wang, Jingjing Lin, Lin Wei, Wei Shen, Yaling Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：STARCH-STARKE 2022
- 关键词：Geobacillus subterraneus pullulanase structure truncation
- A New Strategy for the Enzymatic Degradation of Sudan Dyes in Non-alkaline Conditions被引量：4收藏
- 作者：Min, Liang Zhang, Lei Jiang, Xin Wei, Wei Wei, Dongzhi Wang, Wei
- 机构：E China Univ Sci & Technol;TEDA
- 来源：WATER AIR AND SOIL POLLUTION 2016
- 关键词：Sudan dye Horseradish peroxidase Glucose oxidase Phenols Inactivation Co-expression
- In Silico Rational Design and ProtEIn Engineering of Disulfide Bridges of an α-Amylase from Geobacillus sp. to Improve Thermostability被引量：4收藏
- 作者：Zhang, Heng Zhai, Wenxin Lin, Lin Wang, Ping Xu, Xiangyang Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol;Weigao Shanghai R&D Ctr;Zaozhuang Jie Nuo Enzyme Co Ltd
- 来源：STARCH-STARKE 2021
- 关键词：α ‐ amylase disulfide bonds Geobacillus stearothermophilus immobilized thermostability
- ProtEIn engineering of a cold-adapted rhamnogalacturonan acetylesterase: In vivo functional expression and cinnamyl acetate synthesis被引量：4收藏
- 作者：Tang, Xu-dong Dong, Feng-ying Zhang, Qi-hao Lin, Lin Wang, Ping Xu, Xiang-yang Wei, Wei Wei, Dong-zhi
- 机构：East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci;Natl Engn Res Ctr Nanotechnol;Weigao Shanghai R&D Ctr;Zaozhuang Jienuo Enzyme Co Ltd
- 来源：PROCESS BIOCHEMISTRY 2021
- 关键词：ProtEIn engineering Rhamnogalacturonan acetylesterase Paenibacillus polymyxa Cold-adapted Cinnamyl acetate
- Facile One-Pot Immobilization of a Novel Esterase and Its Application in Cinnamyl Acetate Synthesis被引量：3收藏
- 作者：Dong, Fengying Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Natl Engn Res Ctr Nanotechnol
- 来源：CATALYSIS LETTERS 2020
- 关键词：High thermostability High organic solvents tolerance Esterase Transesterification Cinnamyl acetate
- Computer-Aided ProtEIn Surface Modification Strategy to Improve the Thermostability of α-Amylase被引量：2收藏
- 作者：Zhai, Wenxin Zhu, Mengyu Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：STARCH-STARKE 2025
- 关键词：alpha-amylase protEIn surface engineering thermostability
- Computer-aided rational design strategy based on protEIn surface charge to improve the thermal stability of a novel esterase from Geobacillus jurassicus被引量：2收藏
- 作者：Song, Runfei Zhang, Jin Zhu, Mengyu Lin, Lin Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol
- 来源：BIOTECHNOLOGY LETTERS 2024
- 关键词：Surface charge protEIn engineering Thermostable esterase Site-directed mutagenesis Rational design
- Enhanced Thermal Stability/Activity of Geobacillus jurassicus Esterase by Rational Design and Application in the Synthesis of Cinnamyl Acetate被引量：0收藏
- 作者：Li, Junze Song, Runfei Lin, Lin Li, Tao Yan, Yan Wei, Wei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai Inst Technol;Natl Engn Res Ctr Nanotechnol;Peking Univ Third Hosp
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2025
- 关键词：Esterase Geobacillus jurassicus Thermal stability Rational design Cinnamyl acetate
