成果/Result
- Development of an Engineered Ketoreductase with Simultaneously Improved Thermostability and Activity for Making a Bulky Atorvastatin Precursor被引量：101收藏
- 作者：Gong, Xu-Min Qin, Zhen Li, Fu-Long Zeng, Bu-Bing Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis asymmetric reduction ketoreductase protEIn engineering thermostabilization atorvastatin precursor
- Antioxidant properties of a vegetable-fruit beverage fermented with two Lactobacillus plantarum strains被引量：85收藏
- 作者：Yang, Xiaoxing Zhou, Jiachun Fan, Liqiang Qin, Zhen Chen, Qiming Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：FOOD SCIENCE AND BIOTECHNOLOGY 2018
- 关键词：Lactobacillus plantarum Vegetable-fruit beverage Fermentation Metabolites Antioxidant activities
- Expression and characterization of a novel cold-adapted chitosanase suitable for chitooligosaccharides controllable preparation被引量：71收藏
- 作者：Qin, Zhen Chen, Qiming Lin, Si Luo, Sa Qiu, Yongjun Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：FOOD CHEMISTRY 2018
- 关键词：Chitosanase Chitooligosaccharide Gynuella sunshinyii Chitin conversion Cold-adapted Glycoside hydrolase family 46
- Insights into the nanofiltration separation mechanism of monosaccharides by molecular dynamics simulation被引量：49收藏
- 作者：Yao, Lei Qin, Zhen Chen, Qiming Zhao, Mengyao Zhao, Hefei Ahmad, Waheed Fan, Liqiang Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SC;Univ Nebraska
- 来源：SEPARATION AND PURIFICATION TECHNOLOGY 2018
- 关键词：Monosaccharide Nanofiltration Molecular dynamics simulation Diffusion coefficient Interaction energy
- Biochemical properties of a novel chitosanase from Bacillus amyloliquefaciens and its use in membrane reactor被引量：46收藏
- 作者：Qin, Zhen Luo, Sa Li, Yun Chen, Qiming Qiu, Yongjun Zhao, Liming Jiang, Lihua Zhou, Jiachun
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：LWT-FOOD SCIENCE AND TECHNOLOGY 2018
- 关键词：Chitosanase Chitooligosaccharides Glycoside hydrolase family 46 Enzyme membrane reactor Product selectivity
- One-step immobilization-purification of enzymes by carbohydrate-binding module family 56 tag fusion被引量：45收藏
- 作者：Qin, Zhen Lin, Si Qiu, Yongjun Chen, Qiming Zhang, Yin Zhou, Jiachun Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT;Chengdu Univ
- 来源：FOOD CHEMISTRY 2019
- 关键词：Carbohydrate binding module Immobilized enzyme Packed-bed reactor Fusion protEIn
- High level production of a Bacillus amlyoliquefaciens chitosanase in Pichia pastoris suitable for chitooligosaccharides preparation被引量：41收藏
- 作者：Luo, Sa Qin, Zhen Chen, Qiming Fan, Liqiang Jiang, Lihua Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2020
- 关键词：Chitosanase Chitooligosaccharides Glycoside hydmlase family 46
- Efficient Immobilization of Bacterial GH Family 46 Chitosanase by Carbohydrate-Binding Module Fusion for the Controllable Preparation of Chitooligosaccharides被引量：33收藏
- 作者：Lin, Si Qin, Zhen Chen, Qiming Fan, Liqiang Zhou, Jiachun Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：chitosanase carbohydrate binding module chitooligosaccharide one-step immobilization-purification packed-bed reactor
- Membrane fouling mechanisms and permeate flux decline model in soy sauce microfiltration被引量：18收藏
- 作者：Sun, Ye Qin, Zhen Zhao, Liming Chen, Qiming Hou, Qingyun Lin, Hua Jiang, Lihua Liu, Jinchang Du, Zheng
- 机构：East China Univ Sci & Technol;SCICBT;Yantai Shino Enterprise Foods Co LTD
- 来源：JOURNAL OF FOOD PROCESS ENGINEERING 2018
- 关键词：Membrane fouling - Production efficiency - Fluorine compounds - Optimization - Clarification - Clarifiers - Digital storage - Nutrition
- Structural insights into the catalytic mechanism of a novel glycoside hydrolase family 113 β-1,4-mannanase from Amphibacillus xylanus被引量：16收藏
- 作者：You, Xin Qin, Zhen Yan, Qiaojuan Yang, Shaoqing Li, Yanxiao Jiang, Zhengqiang
- 机构：China Agr Univ;East China Univ Sci & Technol;China Agr Univ
- 来源：JOURNAL OF BIOLOGICAL CHEMISTRY 2018
- 关键词：Binding sites - Hydrolases - Enzyme activity - Hydrolysis - Biochemical engineering - Catalysis
- Efficient inhibition of Cronobacter biofilms by chitooligosaccharides of specific molecular wEIght被引量：14收藏
- 作者：Lu, Jun Chen, Qiming Pan, Bolin Qin, Zhen Fan, Liqiang Xia, Quanming Zhao, Liming
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2019
- 关键词：Cronobacter spp Biofilms Chitooligosaccharides
- Physicochemical Characteristics of Edible Bird's Nest ProtEIns and ThEIr Cooking Processing Properties被引量：8收藏
- 作者：Huang, Sha Zhao, Liming Chen, Qiming Qin, Zhen Zhou, Jiachun Qiu, Yongjun Zhang, Yin Ma, Mingyue
- 机构：East China Univ Sci & Technol;Lishui Acad Agr Sci;SCICBT;Chengdu Univ;Chongqing Univ Posts & Telecommun
- 来源：INTERNATIONAL JOURNAL OF FOOD ENGINEERING 2018
- 关键词：edible bird's nest protEIns extraction method physicochemical and processing properties water-holding capacity
- Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice被引量：3收藏
- 作者：Li, Wei Zhao, Mengyao Qin, Zhen Chen, Qiming Fan, Liqiang Zhou, Jiachun Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT;Huizhou Long Dragon Biotechnol Co Ltd
- 来源：JOURNAL OF FOOD BIOCHEMISTRY 2019
- 关键词：chemical interaction chitooligosaccharides retinol retinol bioavailability retinol metabolism
- 苹果酵素天然发酵过程中代谢产物的变化规律被引量：0收藏
- 作者：杨小幸 周家春 陈启明 秦臻 马明月 赵黎明
- 机构：华东理工大学生物工程学院;重庆邮电大学生物信息学院
- 来源：《食品科学》 2017
- 关键词：苹果酵素 天然发酵 代谢物质 DPPH自由基清除率
- 摘要：为研究苹果酵素天然发酵过程中物质代谢变化,对天然发酵过程中pH值、总酸、有机酸(乳酸、醋酸、苹果酸)、糖类(蔗糖、葡萄糖、果糖)、乙醇、总酚含量、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picryl...
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- 解淀粉芽孢杆菌β-N-乙酰氨基葡萄糖苷酶基因的克隆表达及其酶学表征被引量：0收藏
- 作者：陈宝莉 秦臻 赵黎明
- 机构：华东理工大学生物工程学院
- 来源：《食品科学》 2020
- 关键词：β-N-乙酰氨基葡萄糖苷酶 解淀粉芽孢杆菌 克隆 酶学性质 糖苷水解酶3家族
- 摘要：从解淀粉芽孢杆菌(Bacillus amyloliquefaciens YX-01)中克隆得到一个新型β-N-乙酰氨基葡萄糖苷酶基因(BaNagase),并成功在大肠杆菌中异源表达。BaNagase编码616个氨基酸,与...
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