成果/Result
- Metagenomic sequencing reveals microbial gene catalogue of phosphinothricin-utilized soils in South China被引量：29收藏
- 作者：Lou, Jiali Liu, Min Gu, Jiali Liu, Qinghai Zhao, Li Ma, Yushu Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Huzhou Univ
- 来源：GENE 2019
- 关键词：Phosphinothricin N-acetyltransferase Metagenomics Antibiotic resistance genes Virulence factors Microbial community
- Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system被引量：26收藏
- 作者：Yang, Zeyu Fu, Hengwei Ye, Wenjie Xie, Youyu Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2020
- 关键词：Genes - Acetone - Flow of gases - Catalyst activity - Molecular dynamics - Cloning
- Efficient Asymmetric Synthesis of Ethyl (S)-4-Chloro-3-hydroxybutyrate Using Alcohol Dehydrogenase SmADH31 with High Tolerance of Substrate and Product in a Monophasic Aqueous System被引量：25收藏
- 作者：Yang, Zeyu Ye, Wenjie Xie, Youyu Liu, Qinghai Chen, Rong Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Hangzhou Normal Univ
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2020
- 关键词：asymmetric synthesis ethyl (S)-4-chloro-3-hydroxybutanoate alcohol dehydrogenases high substrate and product tolerance monophasic aqueous system
- Integrated Transcriptome and Proteome Studies Reveal the Underlying Mechanisms for Sterol Catabolism and Steroid Production in Mycobacterium neoaurum被引量：24收藏
- 作者：Liu, Min Xiong, Liang-Bin Tao, Xinyi Liu, Qing-Hai Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：sterol catabolism transcriptome proteome Mycobacterium
- High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro被引量：23收藏
- 作者：Ma, Xingyuan Zheng, Wenyun Wei, Dongzhi Ma, Yushu Wang, Tianwen Wang, Jinzhi Liu, Qinghai Yang, Shengli
- 机构：E China Univ Sci & Technol;Shenyang Pharmaceut Univ;Chinese Acad Sci
- 来源：JOURNAL OF BIOTECHNOLOGY 2006
- 关键词：TAT-survivin (T34A) mutant high-level expression and purification pro-apoptosis
- Large scale preparation of recombinant human parathyroid hormone 1-84 from Escherichia coli被引量：19收藏
- 作者：Liu, Qinghai Lin, Jinping Liu, Meiyun Tao, Xinyi Wei, Dongzhi Ma, Xingyuan Yang, Shengli
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2007
- 关键词：rhPTH(1-84) fusion protein chromatography enterokinase adenylate cyclase large scale preparation Escherichia coli
- Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae被引量：16收藏
- 作者：Tao, Xin-Yi Lin, Yang-Chen Wang, Feng-Qing Liu, Qing-Hai Ma, Yu-Shu Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2022
- 关键词：Patchoulol Mitochondria compartmentation S cerevisiae Metabolic engineering Sesquiterpene
- Metabolic Adaptation of Mycobacterium neoaurum ATCC 25795 in the Catabolism of Sterols for Producing Important Steroid Intermediates被引量：15收藏
- 作者：Liu, Min Xiong, Liang-Bin Tao, Xinyi Liu, Qing-Hai Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：sterol catabolism mycobacteria cell envelope steroids efflux pumps
- Comparison of the expression of phospholipase D from Streptomyces halstedii in different hosts and its over-expression in Streptomyces lividans被引量：14收藏
- 作者：Tao, Xinyi Zhao, Ming Zhang, Ying Liu, Min Liu, Qinghai Wang, Wei Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：FEMS MICROBIOLOGY LETTERS 2019
- 关键词：phospholipase D Streptomyces lividans Pichia pastoris cytotoxic dual-promoter
- Efficient Biocatalytic Synthesis of (R)-2-Chloro-1-(3,4-difluorophenyl)ethanol by the Short-Chain Dehydrogenase PpKR8 from Paraburkholderia phymatum STM815被引量：14收藏
- 作者：He, Ling Ye, Wenjie Xie, Youyu Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2022
- 关键词：(R)-2-chloro-1-(3,4-difluorophenyl)ethanol alcohol dehydrogenase asymmetric reduction coexpression
- Characterization of a novel nitrilase, BGC4, from Paraburkholderia graminis showing wide-spectrum substrate specificity, a potential versatile biocatalyst for the degradation of nitriles被引量：13收藏
- 作者：Fan, Haiyang Chen, Lifeng Sun, Huihui Wang, Hualei Liu, Qinghai Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chinese Acad Fishery Sci Qingdao
- 来源：BIOTECHNOLOGY LETTERS 2017
- 关键词：Biodegradation Nitrilase Paraburkholderia graminis Wide-spectrum substrate specificity
- Development of a fed-batch process for the production of anticancer drug TATm-survivin(T34A) in Escherichia coli被引量：10收藏
- 作者：Zhang, Haiyi Zheng, Yu Liu, Qinghai Tao, Xinyi Zheng, Wenyun Ma, Xingyuan Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2009
- 关键词：TAT(m)-survivin(T34A) Escherichia coli Fed-batch culture Optimization Acetic acid Bioprocess design
- The identification and application of a robust ω-transaminase with high tolerance towards substrates and isopropylamine from a directed soil metagenome被引量：9收藏
- 作者：Xie, Youyu Wang, Jiguo Yang, Lin Wang, Wei Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2022
- 关键词：Amines - Efficiency - Ketones - Reaction kinetics - Molecular dynamics
- Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile被引量：8收藏
- 作者：Fan, Haiyang Chen, Lifeng Sun, Huihui Wang, Hualei Liu, Qinghai Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chinese Acad Fishery Sci
- 来源：CHEMICAL PAPERS 2017
- 关键词：Biotransformation Fed-batch reaction Fermentation Nitrilase Phenylacetic acid
- Semirational Engineering of a Distal Loop Region to Enhance the Catalytic Activity and Stability of Leucine Dehydrogenase被引量：4收藏
- 作者：Li, Rui Xu, Minglu Xu, Meng Zhou, Yiquan Wen, Tao Liu, Qinghai Yi, Dong Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;China State Inst Pharmaceut Ind
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2025
- 关键词：
l -phenylglycine distant modulation loop engineering benzoylformicacid enantiomericexcess triple-point mutation formate dehydrogenase molecular dynamics simulations
- Inside-Out Rational Design of Ornithine Cyclodeaminase RlOCD from Rhizobium leguminosarum by a Multiregion Synergy Strategy for Efficient Synthesis of l-Pipecolic Acid被引量：3收藏
- 作者：Gao, Weijie Jing, Zijian Meng, Yifang Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2024
- 关键词：
l -pipecolic acid ornithine cyclodeaminase rational design
- Preparation and purification of novel phosphatidyl prodrug and performance modulation of phosphatidyl nanoprodrug被引量：2收藏
- 作者：Niu, Rui Zhang, PeiLei Wang, Feng-Qing Liu, Min Liu, QingHai Jia, Ning Yang, ShengLi Tao, XinYi Wei, DongZhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2019
- 关键词：Phosphatidyl prodrug Purification Gradient elution Silica gel column chromatography PEGylation RGD modification
- Substrate-Cofactor Synergistic Reorientation and Substrate Tunnel Engineering of SspLCD for Efficient l-Pipecolic Acid Biosynthesis被引量：2收藏
- 作者：Gao, Weijie Huang, Ziyan Li, Yanjun Sun, Shuya Liu, Qinghai Wang, Hualei Chen, Rong Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Hangzhou Normal Univ
- 来源：ORGANIC LETTERS 2025
- Enhancing Alcohol Dehydrogenase Activity for the Efficient Synthesis of (S)-2-Chloro-1-(2,4-dichlorophenyl)ethanol Using Computer-Aided Combinational Mutagenesis被引量：1收藏
- 作者：Ye, Wenjie Xie, Jingwen Gao, Weijie Meng, Yifang Liu, Qinghai Wang, Hualei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Shanghai ZJ Biotech Co Ltd
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2025
- 关键词：alcohol dehydrogenase enzyme engineering virtual saturation mutagenesis combinational mutagenesis (S)-2-chloro-1-(2,4-dichlorophenyl)ethanol
- Metabolic engineering of Escherichia coli for efficient production of O-succinyl-L-homoserine被引量：0收藏
- 作者：Liu, Longmei Gu, Jieyun Lyu, Xiaomei Gu, JiaLi Liu, Qinghai Ma, Yu-Shu Tao, Xin-Yi Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Jiangnan Univ;Huzhou Univ
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2026
- 关键词：O-Succinyl-L-homoserine Escherichia coli Self-regulating promoter Metabolic engineering Flagellar system
- Application of comparative proteome analysis to reveal influence of cultivation conditions on asymmetric bioreduction of β-keto ester by Saccharomyces cerevisiae被引量：0收藏
- 作者：Lin, Jinping Liu, Qinghai Su, Erzheng Wei, Dongzhi Yang, Shengli
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2008
- 关键词：asymmetric bioreduction Saccharomyces cerevisiae growth medium ethyl benzoylacetate proteome analysis
- Large scale preparation and characterization of a genetically engineering protein and biological activity of suppressing proliferation and rapid pro-apoptosis to carcinoma cells被引量：0收藏
- 作者：Ma, Xingyuan Zheng, Wenyun Zhang, Haiyi Tao, Xinyi Liu, Qinghai Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2008
- Rational engineering of Corynebacterium glutamicum for L-Glutamine biosynthesis被引量：0收藏
- 作者：Lin, Ying-Tong Chai, Meng Liu, Qing-Hai Ma, Yu-Shu Tao, Xin-Yi Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2026
- 关键词：L-Glutamine Metabolic engineering RBS engineering SRNA interference Growth-responsive promoter
