成果/Result
- Biomarkers of aging被引量：333收藏
- 作者：Aging Biomarker Consortium Bao, Hainan Cao, Jiani Chen, Mengting Chen, Min Chen, Wei Chen, Xiao Chen, Yanhao Chen, Yu Chen, Yutian Chen, Zhiyang Chhetri, Jagadish K. Ding, Yingjie Feng, Junlin Guo, Jun Guo, Mengmeng He, Chuting Jia, Yujuan Jiang, Haiping Jing, Ying Li, Dingfeng Li, Jiaming Li, Jingyi Liang, Qinhao Liang, Rui Liu, Xiaoqian Liu, Zuojun Luo, Oscar Junhong Lv, Jianwei Ma, Jingyi Mao, Kehang Nie, Jiawei Qiao, Xinhua Sun, Xinpei Tang, Xiaoqiang Wang, Jianfang Wang, Qiaoran Wang, Siyuan Wang, Xuan Wang, Yaning Wang, Yuhan Wu, Rimo Xia, Kai Xiao, Fu-Hui Xu, Lingyan Xu, Yingying Yan, Haoteng Yang, Liang Yang, Ruici Yang, Yuanxin Ying, Yilin Zhang, Le Zhang, Weiwei Zhang, Wenwan Zhang, Xing Zhang, Zhuo Zhou, Min Zhou, Rui Zhu, Qingchen Zhu, Zhengmao Cao, Feng Cao, Zhongwei Chan, Piu Chen, Chang Chen, Guobing Chen, Hou-Zao Chen, Jun Ci, Weimin Ding, Bi-Sen Ding, Qiurong Gao, Feng Han, Jing-Dong J. Huang, Kai Ju, Zhenyu Kong, Qing-Peng Li, Ji Li, Jian Li, Xin Liu, Baohua Liu, Feng Liu, Lin Liu, Qiang Liu, Xingguo Liu, Yong Luo, Xianghang Ma, Shuai Ma, Xinran Mao, Zhiyong Nie, Jing Peng, Yaojin Qu, Jing Ren, Jie Ren, Ruibao Song, Moshi Zhou Songyang Sun, Yi Eve Sun, Yu Tian, Mei Wang, Shusen Wang, Si Wang, Xia Wang, Xiaoning Wang, Yan-Jiang Wang, Yunfang Wong, Catherine C. L. Xiang, Andy Peng Xiao, Yichuan Xie, Zhengwei Xu, Daichao Ye, Jing Yue, Rui Zhang, Cuntai Zhang, Hongbo Zhang, Liang Zhang, Weiqi Zhang, Yong Zhang, Yun-Wu Zhang, Zhuohua Zhao, Tongbiao Zhao, Yuzheng Zhu, Dahai Zou, Weiguo Pei, Gang Liu, Guang-Hui
- 机构：Aging Biomarker Consortium ABC;Chinese Acad Sci;China Natl Ctr Bioinformat;Chinese Acad Sci;Cent South Univ
- 来源：SCIENCE CHINA-LIFE SCIENCES 2023
- 关键词：aging senescence biomarker clock
- 下载全文在线阅读
- SoNar, a Highly Responsive NAD+/NADH Sensor, Allows High-Throughput Metabolic Screening of Anti-tumor Agents被引量：321收藏
- 作者：Zhao, Yuzheng Hu, Qingxun Cheng, Feixiong Su, Ni Wang, Aoxue Zou, Yejun Hu, Hanyang Chen, Xianjun Zhou, Hai-Meng Huang, Xinzhi Yang, Kai Zhu, Qian Wang, Xue Yi, Jing Zhu, Linyong Qian, Xuhong Chen, Lixin Tang, Yun Loscalzo, Joseph Yang, Yi
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;E China Univ Sci & Technol;E China Univ Sci & Technol;Tsinghua Univ
- 来源：CELL METABOLISM 2015
- Genetically encoded fluorescent sensors reveal dynamic regulation of NADPH metabolism被引量：269收藏
- 作者：Tao, Rongkun Zhao, Yuzheng Chu, Huanyu Wang, Aoxue Zhu, Jiahuan Chen, Xianjun Zou, Yejun Shi, Mei Liu, Renmei Su, Ni Du, Jiulin Zhou, Hai-Meng Zhu, Linyong Qian, Xuhong Liu, Haiyan Loscalzo, Joseph Yang, Yi
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;East China Univ Sci & Technol;Univ Sci & Technol China;Tsinghua Univ
- 来源：NATURE METHODS 2017
- Regulation of G6PD acetylation by SIRT2 and KAT9 modulates NADPH homeostasis and cell survival during oxidative stress被引量：257收藏
- 作者：Wang, Yi-Ping Zhou, Li-Sha Zhao, Yu-Zheng Wang, Shi-Wen Chen, Lei-Lei Liu, Li-Xia Ling, Zhi-Qiang Hu, Fu-Jun Sun, Yi-Ping Zhang, Jing-Ye Yang, Chen Yang, Yi Xiong, Yue Guan, Kun-Liang Ye, Dan
- 机构：Fudan Univ;Fudan Univ;E China Univ Sci & Technol;Chinese Acad Sci;Chinese Acad Sci
- 来源：EMBO JOURNAL 2014
- 关键词：acetylation G6PD nicotinamide adenine dinucleotide phosphate reactive oxygen species SIRT2
- Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs被引量：249收藏
- 作者：Chen, Xianjun Zhang, Dasheng Su, Ni Bao, Bingkun Xie, Xin Zuo, Fangting Yang, Lipeng Wang, Hui Jiang, Li Lin, Qiuning Fang, Mengyue Li, Ningfeng Hua, Xin Chen, Zhengda Bao, Chunyan Xu, Jinjin Du, Wenli Zhang, Lixin Zhao, Yuzheng Zhu, Linyong Loscalzo, Joseph Yang, Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：NATURE BIOTECHNOLOGY 2019
- 关键词：cells - Enzyme kinetics - Fluorescence - Mammals - Cytology
- Genetically Encoded Fluorescent Sensors for Intracellular NADH Detection被引量：238收藏
- 作者：Zhao, Yuzheng Jin, Jing Hu, Qingxun Zhou, Hai-Meng Yi, Jing Yu, Zhenhang Xu, Lei Wang, Xue Yang, Yi Loscalzo, Joseph
- 机构：E China Univ Sci & Technol;Tsinghua Univ;Shanghai Jiao Tong Univ;China Natl Ctr Biotechnol Dev;Harvard Univ
- 来源：CELL METABOLISM 2011
- SIRT5 inhibits peroxisomal ACOX1 to prevent oxidative damage and is downregulated in liver cancer被引量：226收藏
- 作者：Chen, Xiu-Fei Tian, Meng-Xin Sun, Ren-Qiang Zhang, Meng-Li Zhou, Li-Sha Jin, Lei Chen, Lei-Lei Zhou, Wen-Jie Duan, Kun-Long Chen, Yu-Jia Gao, Chao Cheng, Zhou-Li Wang, Fang Zhang, Jin-Ye Sun, Yi-Ping Yu, Hong-Xiu Zhao, Yu-Zheng Yang, Yi Liu, Wei-Ren Shi, Ying-Hong Xiong, Yue Guan, Kun-Liang Ye, Dan
- 机构：Shanghai Med Coll;Fudan Univ;Fudan Univ;Fudan Univ;Minist Educ
- 来源：EMBO REPORTS 2018
- 关键词：ACOX1 liver cancer oxidative stress SIRT5 succinylation
- SIRT3-dependent GOT2 acetylation status affects the malate-aspartate NADH shuttle activity and pancreatic tumor growth被引量：167收藏
- 作者：Yang, Hui Zhou, Lisha Shi, Qian Zhao, Yuzheng Lin, Huaipeng Zhang, Mengli Zhao, Shimin Yang, Yi Ling, Zhi-Qiang Guan, Kun-Liang Xiong, Yue Ye, Dan
- 机构：Fudan Univ;E China Univ Sci & Technol;Zhejiang Prov Canc Hosp;Univ Calif San Diego;Univ Calif San Diego
- 来源：EMBO JOURNAL 2015
- 关键词：acetylation GOT2 malate-aspartate NADH shuttle pancreatic cancer
- PDK4-dependent hypercatabolism and lactate production of senescent cells promotes cancer malignancy被引量：154收藏
- 作者：Dou, Xuefeng Fu, Qiang Long, Qilai Liu, Shuning Zou, Yejun Fu, Da Xu, Qixia Jiang, Zhirui Ren, Xiaohui Zhang, Guilong Wei, Xiaoling Li, Qingfeng Campisi, Judith Zhao, Yuzheng Sun, Yu
- 机构：Univ Chinese Acad Sci;Binzhou Med Univ;Fudan Univ;East China Univ Sci & Technol;Chinese Acad Med Sci
- 来源：NATURE METABOLISM 2023
- A genome-wide positioning systems network algorithm for in silico drug repurposing被引量：136收藏
- 作者：Cheng, Feixiong Lu, Weiqiang Liu, Chuang Fang, Jiansong Hou, Yuan Handy, Diane E. Wang, Ruisheng Zhao, Yuzheng Yang, Yi Huang, Jin Hill, David E. Vidal, Marc Eng, Charis Loscalzo, Joseph
- 机构：Cleveland Clin;Case Western Reserve Univ;Case Western Reserve Univ;East China Normal Univ;East China Normal Univ
- 来源：NATURE COMMUNICATIONS 2019
- In vivo monitoring of cellular energy metabolism using SoNar, a highly responsive sensor for NAD+/NADH redox state被引量：136收藏
- 作者：Zhao, Yuzheng Wang, Aoxue Zou, Yejun Su, Ni Loscalzo, Joseph Yang, Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;Harvard Med Sch
- 来源：NATURE PROTOCOLS 2016
- A redox mechanism underlying nucleolar stress sensing by nucleophosmin被引量：130收藏
- 作者：Yang, Kai Wang, Ming Zhao, Yuzheng Sun, Xuxu Yang, Yi Li, Xie Zhou, Aiwu Chu, Huilin Zhou, Hu Xu, Jianrong Wu, Mian Yang, Jie Yi, Jing
- 机构：Shanghai Jiao Tong Univ;East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Shanghai Inst Mat Med;Shanghai Jiao Tong Univ
- 来源：NATURE COMMUNICATIONS 2016
- Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease被引量：106收藏
- 作者：Li, Xi Zhang, Yinan Xu, Lingya Wang, Aoxue Zou, Yejun Li, Ting Huang, Li Chen, Weicai Liu, Shuning Jiang, Kun Zhang, Xiuze Wang, Dongmei Zhang, Lijuan Zhang, Zhuo Zhang, Zeyi Chen, Xianjun Jia, Wei Zhao, Aihua Yan, Xinfeng Zhou, Haimeng Zhu, Linyong Ma, Xinran Ju, Zhenyu Jia, Weiping Wang, Congrong Loscalzo, Joseph Yang, Yi Zhao, Yuzheng
- 机构：East China Univ Sci & Technol;Chinese Acad Med Sci;Chinese Acad Sci;Shanghai Jiao Tong Univ Affiliated Peoples Hosp 6;East China Normal Univ
- 来源：CELL METABOLISM 2023
- Illuminating NAD+ Metabolism in Live cells and In Vivo Using a Genetically Encoded Fluorescent Sensor被引量：95收藏
- 作者：Zou, Yejun Wang, Aoxue Huang, Li Zhu, Xudong Hu, Qingxun Zhang, Yinan Chen, Xianjun Li, Fengwen Wang, Qiaohui Wang, Hu Liu, Renmei Zuo, Fangting Li, Ting Yao, Jing Qian, Yajin Shi, Mei Yue, Xiao Chen, Weicai Zhang, Zhuo Wang, Congrong Zhou, Yong Zhu, Linyong Ju, Zhenyu Loscalzo, Joseph Yang, Yi Zhao, Yuzheng
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;East China Univ Sci & Technol;Hangzhou Normal Univ;Shanghai Jiao Tong Univ
- 来源：DEVELOPMENTAL CELL 2020
- Analysis of redox landscapes and dynamics in living cells and in vivo using genetically encoded fluorescent sensors被引量：83收藏
- 作者：Zou, Yejun Wang, Aoxue Shi, Mei Chen, Xianjun Liu, Renmei Li, Ting Zhang, Chenxia Zhang, Zhuo Zhu, Linyong Ju, Zhenyu Loscalzo, Joseph Yang, Yi Zhao, Yuzheng
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;Jinan Univ;Harvard Med Sch
- 来源：NATURE PROTOCOLS 2018
- The alternative activity of nuclear PHGDH contributes to tumour growth under nutrient stress被引量：70收藏
- 作者：Ma, Chunmin Zheng, Ke Jiang, Kun Zhao, Qin Sha, Nannan Wang, Wang Yan, Man Chen, Tao Zhao, Yuzheng Jiang, Yuhui
- 机构：Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ;East China Univ Sci & Technol;Chinese Acad Med Sci
- 来源：NATURE METABOLISM 2021
- Lactate regulates major zygotic genome activation by H3K18 lactylation in mammals被引量：67收藏
- 作者：Li, Jingyu Hou, Weibo Zhao, Qi Han, Wei Cui, Hongdi Xiao, Songling Zhu, Ling Qu, Jiadan Liu, Xiaoyu Cong, Weitao Shen, Jingling Zhao, Yuzheng Gao, Shaorong Huang, Guoning Kong, Qingran
- 机构：Chongqing Med Univ;Wenzhou Med Univ;Tongji Univ;Wenzhou Med Univ;Wenzhou Univ
- 来源：NATIONAL SCIENCE REVIEW 2024
- 关键词：mammalian preimplantation development lactate epigenetic remodeling histone lactylation zygotic genome activation
- A fine-tuning mechanism underlying self-control for autophagy: deSUMOylation of BECN1 by SENP3被引量：65收藏
- 作者：Liu, Kejia Guo, Chu Lao, Yimin Yangb, Jie Chen, Fei Zhao, Yuzheng Yang, Yi Yang, Jie Yi, Jing
- 机构：Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ;East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci
- 来源：AUTOPHAGY 2020
- 关键词：Autophagy BECN1 reactive oxygen species SENP3 SUMOylation
- Lighting Up Live-cell and In Vivo central Carbon Metabolism with Genetically Encoded Fluorescent Sensors被引量：63收藏
- 作者：Zhang, Zhuo Cheng, Xiawei Zhao, Yuzheng Yang, Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci
- 来源：ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 13 2020
- 关键词：central carbon metabolism genetically encoded fluorescent sensors real-time monitoring fluorescence imaging
- Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins被引量：60收藏
- 作者：Liu, Renmei Yang, Jing Yao, Jing Zhao, Zhou He, Wei Su, Ni Zhang, Zeyi Zhang, Chenxia Zhang, Zhuo Cai, Haibo Zhu, Linyong Zhao, Yuzheng Quan, Shu Chen, Xianjun Yang, Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci
- 来源：NATURE BIOTECHNOLOGY 2022
- 关键词：Proteins - cell culture
- Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells被引量：58收藏
- 作者：Jiang, Li Xie, Xin Su, Ni Zhang, Dasheng Chen, Xianjun Xu, Xiaochen Zhang, Bibi Huang, Kaiyi Yu, Jingwei Fang, Mengyue Bao, Bingkun Zuo, Fangting Yang, Lipeng Zhang, Rui Li, Huiwen Huang, Xinyi Chen, Zhengda Zeng, Qingmei Liu, Renmei Lin, Qiuning Zhao, Yuzheng Ren, Aiming Zhu, Linyong Yang, Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Fluorescence Diag Shanghai Biotech Co Ltd;Zhejiang Univ
- 来源：NATURE METHODS 2023
- PPM1K Regulates Hematopoiesis and Leukemogenesis through CDC20-Mediated Ubiquitination of MEIS1 and p21被引量：57收藏
- 作者：Liu, Xiaoye Zhang, Feifei Zhang, Yaping Li, Xie Chen, Chiqi Zhou, Meiyi Yu, Zhuo Liu, Yunxia Zhao, Yuzheng Hao, Xiaoxin Tang, Yabin Zhu, Liang Liu, Ligen Xie, Li Gu, Hao Shao, Hongfang Xia, Fangzhen Yin, Chunrong Tao, Minfang Xie, Jingjing Zhang, Cheng Cheng Yang, Yi Sun, Haipeng Chen, Guo-Qiang Zheng, Junke
- 机构：Shanghai Jiao Tong Univ;Chinese Acad Sci;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ;Shanghai Key Lab Orbital Dis & Ocular Oncol
- 来源：CELL REPORTS 2018
- SIRT5 deficiency suppresses mitochondrial ATP production and promotes AMPK activation in response to energy stress被引量：56收藏
- 作者：Zhang, Mengli Wu, Jian Sun, Renqiang Tao, Xiaoting Wang, Xiaoxia Kang, Qi Wang, Hui Zhang, Lei Liu, Peng Zhang, Jinye Xia, Yukun Zhao, Yuzheng Yang, Yi Xiong, Yue Guan, Kun-Liang Zou, Yunzeng Ye, Dan
- 机构：Fudan Univ;Fudan Univ;Fudan Univ;Fudan Univ;Fudan Univ
- 来源：PLOS ONE 2019
- The Aging Biomarker Consortium represents a new era for aging research in China被引量：54收藏
- 作者：Ren, Jie Song, Moshi Zhang, Weiqi Cai, Jian-Ping Cao, Feng Cao, Zhongwei Chan, Piu Chen, Chang Chen, Guobing Chen, Hou-Zao Chen, Jun Chen, Xiao-Chun Ci, Weimin Ding, Bi-Sen Ding, Qiurong Gao, Feng Gao, Shaorong Han, Jing-Dong J. He, Qi-Yang Huang, Kai Ju, Zhenyu Kong, Qing-Peng Li, Ji Li, Jian Li, Jingyi Li, Xin Liu, Baohua Liu, Feng Liu, Jun-Ping Liu, Lin Liu, Qiang Liu, Xingguo Liu, Yong Luo, Xianghang Ma, Shuai Ma, Xinran Mao, Zhiyong Nie, Jing Peng, Yaojin Qu, Jing Ren, Ruibao Song, Weihong Songyang, Zhou Sun, Liang Sun, Yi Eve Sun, Yu Tian, Mei Tian, Xiao-Li Tian, Ye Wang, Jianwei Wang, Shusen Wang, Si Wang, Wengong Wang, Xia Wang, Xiaoning Wang, Yan-Jiang Wang, Yunfang Wong, Catherine C. L. Xiang, Andy Peng Xiao, Yichuan Xiao, Zhi-Xiong Xie, Zhengwei Xiong, Wei Xu, Daichao Yang, Ze Ye, Jing Yu, Wei Yue, Rui Zhang, Cuntai Zhang, Hongbo Zhang, Liang Zhang, Xinchao Zhang, Yong Zhang, Yun-Wu Zhang, Zhuohua Zhao, Tongbiao Zhao, Yuzheng Zhou, Zhongjun Zhu, Dahai Zou, Weiguo Pei, Gang Liu, Guang-Hui
- 机构：Chinese Acad Sci;Chinese Acad Sci;China Natl Ctr Bioinformat;Univ Chinese Acad Sci;Chinese Acad Sci
- 来源：NATURE MEDICINE 2023
- Bone marrow niche ATP levels determine leukemia-initiating cell activity via P2X7 in leukemic models被引量：54收藏
- 作者：He, Xiaoxiao Wan, Jiangbo Yang, Xiaona Zhang, Xiuze Huang, Dan Li, Xie Zou, Yejun Chen, Chiqi Yu, Zhuo Xie, Li Zhang, Yaping Liu, Ligen Li, Shangang Zhao, Yuzheng Shao, Hongfang Yu, Ye Zheng, Junke
- 机构：Shanghai Ciao Tong Univ;Shanghai Pao Tong Univ;China Pharmaceut Univ;East China Univ Sci & Technol;Kunming Univ Sci & Technol
- 来源：JOURNAL OF CLINICAL INVESTIGATION 2021
- Colon-targeted delivery of tacrolimus using pH-responsive polymeric nanoparticles for murine colitis therapy被引量：53收藏
- 作者：Cai, Xiaoran Wang, Xiaolei He, Muye Wang, Yan Lan, Minbo Zhao, Yuzheng Gao, Feng
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF PHARMACEUTICS 2021
- 关键词：Oral nanoparticle delivery system pH-responsive Targeted drug delivery Tacrolimus Inflammatory bowel diseases
- Real-time and high-throughput analysis of mitochondrial metabolic states in living cells using genetically encoded NAD+/NADH sensors被引量：53收藏
- 作者：Zhao, Yuzheng Yang, Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：FREE RADICAL BIOLOGY AND MEDICINE 2016
- 关键词：Mitochondria Cytosol Metabolic state Genetically encoded fluorescent sensor Imaging High-throughput NADH NAD(+)/NADH ratio
- A single-component light sensor system allows highly tunable and direct activation of gene expression in bacterial cells被引量：52收藏
- 作者：Li, Xie Zhang, Changcheng Xu, Xiaopei Miao, Jun Yao, Jing Liu, Renmei Zhao, Yuzheng Chen, Xianjun Yang, Yi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci;East China Univ Sci & Technol
- 来源：NUCLEIC ACIDS RESEARCH 2020
- Metabolic Imaging Reveals a Unique Preference of Symmetric cell Division and Homing of Leukemia-Initiating cells in an Endosteal Niche被引量：49收藏
- 作者：Hao, Xiaoxin Gu, Hao Chen, Chiqi Huang, Dan Zhao, Yuzheng Xie, Li Zou, Yejun Shu, Hui Sophie Zhang, Yaping He, Xiaoxiao Lai, Xiaoyun Zhang, Xiaocui Zhou, Bo O. Zhang, Cheng Cheng Chen, Guo-Qiang Yu, Zhuo Yang, Yi Zheng, Junke
- 机构：Shanghai Jiao Tong Univ;East China Univ Sci & Technol;Chinese Acad Sci;UT Southwestern Med Ctr;Shanghai Jiao Tong Univ
- 来源：CELL METABOLISM 2019
- Colon-specific delivery of methotrexate using hyaluronic acid modified pH-responsive nanocarrier for the therapy of colitis in mice被引量：47收藏
- 作者：Lv, Yingni Ren, Mengjiao Yao, Min Zou, Jiafeng Fang, Siqi Wang, Yanbing Lan, Minbo Zhao, Yuzheng Gao, Feng
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci
- 来源：INTERNATIONAL JOURNAL OF PHARMACEUTICS 2023
- 关键词：Inflammatory bowel disease Methotrexate Oral nano drug delivery system pH-sensitive Colon-specific Active targeting
