成果/Result
- A cytosensor based on NiO nanoparticle-enhanced surface plasmon resonance for detection of the breast cancer cell line MCF-7被引量：43收藏
- 作者：Jia, Shengsong Li, Pengfei Koh, Kwangnak Chen, Hongxia
- 机构：Shanghai Univ;E China Univ Sci & Technol;Pusan Natl Univ
- 来源：MICROCHIMICA ACTA 2016
- 关键词：Aptamer His tag MUC-1 Diagnosis Tumor marker Islet beta cells Biosensor
- Improved breast cancer cell-specific intracellular drug delivery and therapeutic efficacy by coupling decoration with cell penetrating peptide and SP90 peptide被引量：15收藏
- 作者：Fan, Li-Qiang Du, Guo-Xiu Li, Peng-Fei Li, Ming-Wei Sun, Yao Zhao, Li-Ming
- 机构：East China Univ Sci & Technol
- 来源：BIOMEDICINE & PHARMACOTHERAPY 2016
- 关键词：Cell-penetrating peptide Homing peptide Targeted drug delivery Breast cancer therapy VPR
- Enhanced anti-tumor activity of trichosanthin after combination with a human-derived cell-penetrating peptide, and a possible mechanism of activity被引量：13收藏
- 作者：Lu, Ye-Zhou Li, Peng Fei Li, Yan-Zhong Luo, Fan Guo, Chao Lin, Bin Cao, Xue-Wei Zhao, Jian Wang, Fu-Jun
- 机构：East China Univ Sci & Technol;Shanghai Univ Tradit Chinese Med;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：FITOTERAPIA 2016
- 关键词：Trichosanthin Cell-penetrating peptide Tumor therapy Apoptosis
- Enhancing tumor-specific intracellular delivering efficiency of cell-penetrating peptide by fusion with a peptide targeting to EGFR被引量：13收藏
- 作者：Long The Nguyen Yang, Xu-Zhong Du, Xuan Wang, Jia-Wei Zhang, Rui Zhao, Jian Wang, Fu-Jun Dong, Yang Li, Peng-Fei
- 机构：E China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd;Shanghai Univ Tradit Chinese Med
- 来源：AMINO ACIDS 2015
- 关键词：Cell-penetrating peptide Tumor-homing peptide Targeted drug delivery Growth factor
- Alter codon bias of the P. pastoris genome to overcome a bottleneck in codon optimization strategy development and improve protein expression被引量：11收藏
- 作者：Zhang, Siyu Lin, Ru Cui, Luyao Jiang, Tianyi Shi, Jiacheng Li, Pengfei Zhou, Mian
- 机构：East China Univ Sci & Technol;China Innovat Ctr Roche
- 来源：MICROBIOLOGICAL RESEARCH 2024
- 关键词：Codon optimization Pichia pastoris Protein expression Chassis cells
- The discovery of new scaffold of plant activators: From salicylic acid to benzotriazole被引量：10收藏
- 作者：Chang, Kang Chen, Jian-Qin Shi, Yan-Xia Sun, Mei-Jian Li, Peng-Fei Zhao, Zhen-Jiang Zhu, Wei-Ping Li, Hong-Lin Xu, Yu-Fang Li, Bao-Ju Qian, Xu-Hong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Agr Sci
- 来源：CHINESE CHEMICAL LETTERS 2017
- 关键词：Plant activator Systemic acquired resistance Salicylic acid Benzotriazole Virtual screening
- Cell-Free Biosensing Genetic Circuit Coupled with Ribozyme Cleavage Reaction for Rapid and Sensitive Detection of Small Molecules被引量：9收藏
- 作者：Li, Wenxing Xu, Yiming Zhang, Yue Li, Pengfei Zhu, Xiaoli Feng, Chang
- 机构：Shanghai Univ;Tongji Univ;East China Univ Sci & Technol;Shanghai Univ
- 来源：ACS SYNTHETIC BIOLOGY 2023
- 关键词：genetic circuit in vitro transcription ribozyme IPTG inhibitory drugs screening 3D-printedsensor array
- 国际基因工程机器大赛对本科生综合能力培养模式的探索被引量：8收藏
- 作者：王启要 李鹏飞 高淑红 李友元 吴辉 谭高翼 范建华 周勉 张立新 庄英萍
- 机构：华东理工大学生物工程学院
- 来源：《生物工程学报》 2021
- 关键词：国际基因工程机器比赛 生物创客 创新能力培养体系 合成生物学
- 摘要：华东理工大学生物工程学院从高水平专业比赛出发,以学生的兴趣为切入点,依托强大的科研和教学基地,构建具有"生物创客"精神的人才培养体系和创新实践能力培养体系。充分发挥学生主观能动性,提升了生物工程类相关专业本科生的科研素养...
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- 同向双螺杆挤压改性对米糠可溶性膳食纤维及营养物质的影响被引量：6收藏
- 作者：代国信 魏永忠 李鹏飞 杨爱民 杜传来 吴酉芝
- 机构：安徽科技学院食品工程学院;上海中侨职业技术大学食品药品学院;华东理工大学生物工程学院;百事亚洲研发中心有限公司
- 来源：《保鲜与加工》 2024
- 关键词：同向双螺杆挤压 工艺优化 米糠改性 可溶性膳食纤维 营养物质
- 摘要：为制备可溶性膳食纤维含量高且营养物质保留好的改性米糠产品,以米糠为原料,可溶性膳食纤维含量为考察指标,在单因素试验结果的基础上,采用响应面法对米糠挤压膨化改性工艺条件进行优化,并对改性前后米糠物性指标(堆积密度、颜色、持...
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- Novel Fluorescence Arginine Analogue as a Sensor for Direct Identification and Imaging of Nitric Oxide Synthase-like Enzymes in Plants被引量：5收藏
- 作者：Chang, Kang Guo, Tongtong Li, Pengfei Liu, Yin Xu, Yufang Fang, Yuda Qian, Xuhong
- 机构：East China Univ Sci & Technol;Chinese Acad Sci
- 来源：SCIENTIFIC REPORTS 2016
- Practices and exploration on competition of molecular biological detection technology among students in food quality and safety major被引量：4收藏
- 作者：Chang, Yaning Peng, Yuke Li, Pengfei Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION 2017
- 关键词：Molecular biological detection undergraduate education pork and beef identification food safety laboratory exercises
- Effectively enhancing cytotoxic and apoptotic effects of alpha-momorcharin by integrating a heparin-binding peptide被引量：4收藏
- 作者：Tan, Meng-Jie Cao, Xue-Wei Li, Peng-Fei Zhai, Yi-Zhou Zhou, Yu Liu, Ye-Jun Zhao, Jian Wang, Fu-Jun
- 机构：East China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd;Shanghai Univ Chinese Tradit Med
- 来源：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 2017
- 关键词：antitumor activity apoptosis cell-penetrating peptides heparin-binding peptide alpha-MMC-HBP
- 基于创新平台的研究型人才培养模式探索被引量：4收藏
- 作者：李鹏飞 赵健 范立强 叶江 吴海珍 张惠展
- 机构：华东理工大学生物工程学院
- 来源：《化工高等教育》 2016
- 关键词：研究型教学模式 创新实验设计 创新思维教学法 自主学习能力 创新人才培养
- 摘要：如何在高校不断扩招及现今"厚基础、宽口径"的教育模式下培养生物科学类专业学术型、创新型优秀本科人才?本项目以大学生创新项目为平台,确立了本科创新人才培养的内涵和多元组织形式,以学生兴趣为切入点,以科学研究思路为导向,将创...
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- Platycodin D reduces PD-L1 levels by inhibiting LXR-β activity and combines with nintedanib to enhance the tumor-killing effect of T cells被引量：2收藏
- 作者：Lei, Jin Cao, Xue-Wei Li, Peng-Fei Zhao, Jian Wang, Fu-Jun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Zhejiang Fonow Med Co Ltd;Shanghai Univ Tradit Chinese Med
- 来源：FEBS LETTERS 2024
- 关键词：cancer therapy cholesterol immunotherapy MHC PD-L1 platycodin D
- Bridging the biochemistry lecture and laboratory courses: Construction and application of the "Innovative Experimental Design" module被引量：1收藏
- 作者：Li, Pengfei Zhang, Boya He, Shuaifei Lu, Yuqing Jiang, Wenli Zhong, Qingsong Quan, Shu Wu, Haizhen Zhou, Mian
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION 2024
- 关键词：biochemistry course development laboratory course microbial fuel cells
- Real-Time Quantitative PCR Analysis of the Expression Pattern of the Hypoglycemic Polypeptide-P Gene in Momordica charantia被引量：1收藏
- 作者：Wang, Yi-Shuai Zeng, Xiang-Qing Yang, Xu-Zhong Liu, Wei Li, Peng-Fei Wang, Fu-Jun Zhao, Jian
- 机构：East China Univ Sci & Technol;Shanghai Univ Tradit Chinese Med;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：GENES 2019
- 关键词：Momordica charantia hypoglycemic polypeptide-P qPCR Western blotting expression pattern
- 项目式教学模式在分子生物学实验课程中的实践与思考被引量：1收藏
- 作者：李鹏飞 吴海珍 范立强 张惠展 马昱澍 叶江
- 机构：华东理工大学生物工程学院应用生物学系
- 来源：《生命的化学》 2025
- 关键词：分子生物学实验 项目式教学模式 教学改革
- 摘要：分子生物学实验课程是生命科学领域的重要实践环节,传统的实验教学多以验证性实验为主,学生往往只是被动地按照既定步骤操作,缺乏主动探索和解决问题的机会。自2021年起,华东理工大学分子生物学实验教学团队引入项目式教学模式,以...
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- A novel CRISPR-Cas9 nickase-mediated rolling circle amplification (CRIRCA) technique for gene identification and quantitative analysis of extrachromosomal DNA被引量：0收藏
- 作者：Song, Yuchen Guan, Chaoyang Zhang, Yue Xu, Yiming Li, Pengfei Luo, Liqiang Feng, Chang Chen, Guifang
- 机构：Shanghai Univ;Shanghai Univ;East China Univ Sci & Technol;Shanghai Univ
- 来源：JOURNAL OF ADVANCED RESEARCH 2026
- 关键词：ecDNA detection CRIRCA CRISPR-Cas9 Tumor markers Breast cancer
- 基于布鲁姆教育目标体系构建分子生物学实验综合考评体系被引量：0收藏
- 作者：叶江 李鹏飞 张惠展 吴海珍 赵健 马昱澍
- 机构：华东理工大学生物工程学院
- 来源：《生命的化学》 2020
- 关键词：分子生物学实验 布鲁姆教育目标体系 综合评价体系 创新能力培养
- 摘要：大学生创新能力培养是一项系统工程,实验实践教学的实施和评价是其中的重要环节。华东理工大学分子生物学教研组以布鲁姆教育目标体系为指导,构建了包括过程评价、结果评价和实践创新能力评价三个模块的分子生物学实验综合考评体系。该考...
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- Machine learning-optimized long single-stranded DNA synthesis technology empowers high-precision diagnostic-therapeutic integration in living cells被引量：0收藏
- 作者：Zhang, Yue Xu, Yiming Ding, Zhihua Cheng, Yutian Gao, Yu Ye, Jiang Zhang, Huizhan Li, Pengfei Wu, Haizhen
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：NUCLEIC ACIDS RESEARCH 2026
- Inception-level signal amplification: Cascaded DNAzyme-Cas9 nickase achieves sub-nanomolar kanamycin tracking被引量：0收藏
- 作者：Xu, Wen Cheng, Yutian Sun, Kexin Wu, Yue Xu, Yiming Ye, Jiang Li, Pengfei Wu, Haizhen
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ANALYTICA CHIMICA ACTA 2026
- 关键词：Kanamycin Aptamer DNAzyme Cas9 nickase
- Metabolic engineering of Bacillus subtilis for high-yield surfactin production被引量：0收藏
- 作者：Wang, Yong Li, Hanqing Mao, Yue Li, Pengfei Wu, Haizhen Ye, Jiang Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 2026
- 关键词：Surfactin Bacillus subtilis Metabolic engineering P43 promoter Tolerance engineering
