成果/Result
- Effective cancer immunotherapy by Ganoderma lucidum polysaccharide-gold nanocomposites through dendritic cell activation and memory T cell response被引量：122收藏
- 作者：Zhang, Shulei Pang, Guibin Chen, Chao Qin, Jianzhong Yu, Huan Liu, Yongming Zhang, Xihui Song, Zhentao Zhao, Jian Wang, Fujun Wang, Yangyun Zhang, Leshuai W.
- 机构：Shanghai Univ Tradit Chinese Med;Soochow Univ;Changshu 2 Peoples Hosp;Soochow Univ;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：CARBOHYDRATE POLYMERS 2019
- 关键词：Polysaccharides Dendritic cell Nanoparticle Immunoregulation Cancer therapy
- Comparison of immunoregulatory effects of polysaccharides from three natural herbs and cellular uptake in dendritic cells被引量：67收藏
- 作者：Zhu, Ning Lv, Xiaochen Wang, Yangyun Li, Jingling Liu, Yongming Lu, Wenfei Yang, Liecheng Zhao, Jian Wang, Fujun Zhang, Leshuai W.
- 机构：Shanghai Univ Tradit Chinese Med;Soochow Univ;Soochow Univ;Zhejiang Reachall Pharmaceut Co Ltd;East China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2016
- 关键词：Natural herbs Polysaccharides Dendritic cell Nitric oxide Endocytosis
- Enhanced cellulase production by decreasing intercellular pH through H+-ATPase gene deletion in Trichoderma reesEI RUT-C30被引量：58收藏
- 作者：Liu, Pei Zhang, Guoxiu Chen, Yumeng Zhao, Jian Wang, Wei Wei, Dongzhi
- 机构： State Key Lab of Bioreactor Engineering
- 来源：Biotechnology for Biofuels 2019
- 关键词：Ammonia - Lignocellulose - Calcium - Carbon - Genes - Industrial chemicals
- Comparative studies on the immunoregulatory effects of three polysaccharides using high content imaging system被引量：53收藏
- 作者：Lv, Xiaocheng Chen, Dandan Yang, Liecheng Zhu, Ning Li, Jingling Zhao, Jian Hua, Zhibi Wang, Fu-Jun Zhang, Leshuai W.
- 机构：Shanghai Univ Tradit Chinese Med;Soochow Univ;Zhejiang Reachall Pharmaceut Co Ltd;E China Univ Sci & Technol;Shanghai R&D Ctr Standardizat Chinese Med
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2016
- 关键词：Polysaccharide Natural products Immunoregulation Cell irregularity Nitrogen oxide Macrophage
- Improving combination cancer immunotherapy by manipulating dual immunomodulatory signals with enzyme-triggered, cell-penetrating peptide-mediated biomodulators被引量：43收藏
- 作者：Pang, Guibin Chen, Piao Cao, Xuewei Yu, Huan Zhang, Leshuai W. Zhao, Jian Wang, Fu-Jun
- 机构： State Key Laboratory of Bioreactor Engineering; New Drug R&D Center; ECUST-FONOW Joint Research Center for Innovative Medicines; Institute of Chinese Materia Medica; School for Radiological and Interdisciplinary Sciences
- 来源：Biomaterials Science 2023
- 关键词：Antigen-antibody reactions - Cell proliferation - Chemical activation - Diseases - Macrophages - Peptides - Scaffolds (biology) - Tumors
- Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010被引量：38收藏
- 作者：Zhao, Li-Li Xu, Jian-He Zhao, Jian Pan, Jiang Wang, Zhi-Long
- 机构： Laboratory of Biocatalysis and Bioprocessing; School of Pharmacy
- 来源：Process Biochemistry 2008
- 关键词：Bacteria - Chirality - Dimers - Enantiomers - Enzyme activity - Organic solvents
- Refolding, purification and characterization of an organic solvent-tolerant lipase from Serratia marcescens ECU1010被引量：37收藏
- 作者：Li, Suxia Pang, Huaiyu Lin, Kang Xu, Jianhe Zhao, Jian Fan, Liqiang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Cloning - Chromatography - Recombinant protEIns - Purification - Organic solvents
- Expression and Characterization of a Novel Enantioselective Lipase from Aspergillus fumigatus被引量：34收藏
- 作者：Shangguan, Jiao-Jiao Fan, Li-Qiang Ju, Xin Zhu, Qing-Qing Wang, Fu-Jun Zhao, Jian Xu, Jian-He
- 机构： State Key Laboratory of Bioreactor Engineering; Institute of Chinese Materia Medica
- 来源：Applied Biochemistry and Biotechnology 2012
- 关键词：Aspergillus - Cloning - Fatty acids - Purification - Escherichia coli - Enantioselectivity
- Significantly improved expression and biochemical properties of recombinant serratia marcescens lipase as robust biocatalyst for kinetic resolution of chiral ester被引量：33收藏
- 作者：Wang, Yi Zhao, Jian Xu, Jian-He Fan, Li-Qiang Li, Su-Xia Zhao, Li-Li Mao, Xiao-Bo
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Biochemistry and Biotechnology 2010
- 关键词：Esters - Gene expression - Recombinant protEIns - Escherichia coli - Fatty acids - Affinity chromatography - Lipases - Substrates - Cloning - Metal ions - Metals - Nonionic surfactants - Purification
- Expression and Characterization of a Novel Lipase from Aspergillus fumigatus with High Specific Activity被引量：31收藏
- 作者：Shangguan, Jiao-Jiao Liu, Yu-Qiang Wang, Fu-Jun Zhao, Jian Fan, Li-Qiang Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Aspergillus fumigatus Recombinant lipase Expression Characterization Active site
- Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol被引量：29收藏
- 作者：Zhu, Qing-Qing He, Wan-Hong Kong, Xu-Dong Fan, Li-Qiang Zhao, Jian Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：RT-PCR Vigna radiata epoxide hydrolase p-Nitrostyrene oxide Enantioconvergency Characterization
- Switching a Newly Discovered Lactonase into an Efficient and Thermostable Phosphotriesterase by Simple Double Mutations His250Ile/Ile263Trp被引量：28收藏
- 作者：Luo, Xiao-Jing Kong, Xu-Dong Zhao, Jian Chen, Qi Zhou, Jiahai Xu, Jian-He
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2014
- 关键词：organophosphate phosphotriesterase lactonase site-directed mutagenesis functional switch X-ray crystallography
- Combinatorial Evolution of Phosphotriesterase Toward a Robust Malathion Degrader by Hierarchical Iteration Mutagenesis被引量：25收藏
- 作者：Luo, Xiao-Jing Zhao, Jian Li, Chun-Xiu Bai, Yun-Peng Reetz, Manfred T. Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Max Planck Inst Kohlenforsch;Philipps Univ Marburg
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2016
- 关键词：biocatalysis directed evolution DNA shuffling malathion phosphotriesterase
- Recent research advancements on regioselective nitrilase: fundamental and applicative aspects被引量：19收藏
- 作者：Chen, Zhi Zhao, Jiang Jiang, Shuiqing Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2019
- 关键词：Biocatalysis Regioselective nitrilase Regioselectivity Molecular modifications Industrial applications
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- Statistical Optimizing of Medium for Clavulanic Acid Production by Streptomyces clavuligerus Using Response Surface Methodology被引量：15收藏
- 作者：Feng, Tao Zhao, Jie Chu, Ju Wang, Yong-hong Zhuang, Ying-ping
- 机构：East China Univ Sci & Technol;Sinopharm Weiqida Pharmaceut Co Ltd
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2021
- 关键词：Clavulanic acid Streptomyces clavuligerus Statistical optimization Response surface methodology
- Biocatalytic Properties of a Recombinant Fusarium proliferatum Lactonase with Significantly Enhanced Production by Optimal Expression in Escherichia coli被引量：12收藏
- 作者：Chen, Bing Fan, Li-Qiang Xu, Jian-He Zhao, Jian Zhang, Xian Ouyang, Li-Ming
- 机构：E China Univ Chem Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Levo-lactonase DL-pantoyl lactone Recombinant protEIn Immobilized metal affinity chromatography Biocatalytic property Substrate specificity
- γ-PGA Fermentation by Bacillus subtilis PG-001 with Glucose Feedback Control pH-stat Strategy被引量：11收藏
- 作者：Wang, Jia-Qi Zhao, Jie Xia, Jian-Ye
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2022
- 关键词：gamma-PGA Fermentation Bacillus subtilis pH-stat Alkali consumption
- Endotoxin contamination in ovalbumin as viewed from a nano-immunotherapy perspective被引量：9收藏
- 作者：Pang, Guibin Liu, Yun Wang, Yangyun Wang, Yong Wang, Fujun Zhao, Jian Zhang, Leshuai W.
- 机构：East China Univ Sci & Technol;Shanghai Univ Tradit Chinese Med;Soochow Univ
- 来源：WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY 2022
- 关键词：antigen presentation endotoxin nano-immunotherapy ovalbumin
- Improving nanochemoimmunotherapy efficacy by boosting "eat-me" signaling and downregulating "don't-eat-me" signaling with Ganoderma lucidum polysaccharide-based drug delivery被引量：8收藏
- 作者：Pang, Guibin Wei, Siqi Zhao, Jian Wang, Fu-Jun
- 机构：East China Univ Sci & Technol;Zhejiang Fonow Med Co Ltd;East China Univ Sci & Technol;Shanghai Univ Tradit Chinese Med
- 来源：JOURNAL OF MATERIALS CHEMISTRY B 2023
- 关键词：Antigen-antibody reactions - Cell death - Cell membranes - Cell signaling - Controlled drug delivery - Signaling - T-cells - Targeted drug delivery - Tumors
- A novel trichosanthin fusion protEIn with increased cytotoxicity to tumor cells被引量：8收藏
- 作者：Lin, Bing Yang, Xu-Zhong Cao, Xue-Wei Zhang, Tao-Zhu Wang, Fu-Jun Zhao, Jian
- 机构：East China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd;Shanghai Univ Tradit Chinese Med
- 来源：BIOTECHNOLOGY LETTERS 2017
- 关键词：Antitumor activity Apoptosis Drug delivery Heparin-binding EGF-like growth factor Trichosanthin
- Tandem multimer expression and preparation of hypoglycemic peptide MC6 from Momordica charantia in Escherichia coli被引量：6收藏
- 作者：Wang, Fu-Jun Song, Huai-Lei Wang, Xiao-Meng Zhang, Wen-Ji Wang, Bi-Lian Zhao, Jian Hu, Zhi-Bi
- 机构： Department of Applied Biology; Institute of Chinese Materia Medica; School of Pharmacy
- 来源：Applied Biochemistry and Biotechnology 2012
- 关键词：Gene expression - Escherichia coli - Chlorine compounds - Peptides
- Effectively enhancing cytotoxic and apoptotic effects of alpha-momorcharin by integrating a heparin-binding peptide被引量：4收藏
- 作者：Tan, Meng-Jie Cao, Xue-Wei Li, Peng-Fei Zhai, Yi-Zhou Zhou, Yu Liu, Ye-Jun Zhao, Jian Wang, Fu-Jun
- 机构：East China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd;Shanghai Univ Chinese Tradit Med
- 来源：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 2017
- 关键词：antitumor activity apoptosis cell-penetrating peptides heparin-binding peptide alpha-MMC-HBP
- 猴附睾特异性表达基因LCN6第5号内含子的克隆及分析被引量：2收藏
- 作者：沈佳 张永莲 刘强 赵健
- 机构：华东理工大学生物工程学院;中国科学院上海生物化学与细胞研究所
- 来源：《华东理工大学学报（自然科学版）》 2005
- 关键词：附睾 LCN6 内含子 PCR 进化
- 摘要：用PCR法扩增了猴LCN6基因第5号内含子区的基因组DNA片断,并将该片断克隆到T-载体中,用SouthernBlot的方法鉴定阳性克隆。经测序后与人LCN6基因的第5号内含子相比较,两者的同源性达到了87%。根据对已有...
- 下载全文在线阅读
- Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B被引量：2收藏
- 作者：Li, Suxia Zhang, Luosheng Wu, Qian Xin, Aijie Zhao, Jian Fan, Liqiang
- 机构：E China Univ Sci & Technol
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2011
- 关键词：Procarboxypeptidase B Mutation CystEIne Refolding efficiency Property
- MST1的酵母双杂交及与Salvador体外结合的研究被引量：0收藏
- 作者：张乐帅 赵健
- 机构：华东理工大学应用生物学系
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：MST1 Salvador 酵母双杂交 磷酸化
- 摘要：利用酵母双杂交系统,以鼠M ST 1(M amm alian STE 20-1 ike 1)为诱饵蛋白,在鼠胚胎库中筛选到22个Salvador的片段。通过E.coli表达系统纯化了6个组氨酸(6H is)融合的M ST...
- 下载全文在线阅读
- Strategy of employing plug-and-play vectors and LC-MS screening to facilitate the discovery of natural products using Aspergillus oryzae被引量：0收藏
- 作者：Shi, Hanliang Lin, Beibei Zheng, Mengmeng Gan, Fengyu Lin, Zhi Xin, Xiujuan Zhao, Jian Qu, Xudong An, Faliang
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Wuhan Univ;Shanghai Jiao Tong Univ;East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2025
- 关键词：Plug-and-play vectors LC-MS detection
Aspergillus oryzae Heterologous expression PamyB
