成果/Result
- HIT: linking herbal active ingredients to targets被引量：252收藏
- 作者：Ye, Hao Ye, Li Kang, Hong Zhang, Duanfeng Tao, Lin Tang, Kailin Liu, Xueping Zhu, Ruixin Liu, Qi Chen, Y. Z. Li, Yixue Cao, Zhiwei
- 机构：Tongji Univ;Shanghai Ctr Bioinformat Technol;E China Univ Sci & Technol;NUS Grad Sch Integrat Sci & Engn;Natl Univ Singapore
- 来源：NUCLEIC ACIDS RESEARCH 2011
- High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells被引量：36收藏
- 作者：Wu, Yixiao Bissinger, Thomas Genzel, Yvonne Liu, Xuping Reichl, Udo Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Max Planck Inst Dynam Complex Tech Syst;Shanghai BioEngine Sci Tech Co Ltd;Otto von Guericke Univ
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2021
- 关键词：MDCK cells Influenza A virus ATF-based perfusion Process optimization Lab-scale bioreactors
- Serum-Free Suspension Culture of MDCK Cells for Production of Influenza H1N1 Vaccines被引量：36收藏
- 作者：Huang, Ding Peng, Wen-Juan Ye, Qian Liu, Xu-Ping Zhao, Liang Fan, Li Xia-Hou, Kang Jia, Han-Jing Luo, Jian Zhou, Lin-Ting Li, Bei-Bei Wang, Shi-Lei Xu, Wen-Ting Chen, Ze Tan, Wen-Song
- 机构：E China Univ Sci & Technol;Shanghai Inst Biol Prod Co Ltd
- 来源：PLOS ONE 2015
- Semi-perfusion cultures of suspension MDCK cells enable high cell concentrations and efficient influenza A virus production被引量：35收藏
- 作者：Bissinger, Thomas Fritsch, Johannes Mihut, Adrian Wu, Yixiao Liu, Xuping Genzel, Yvonne Tan, Wen-Song Reichl, Udo
- 机构：Max Planck Inst Dynam Complex Tech Syst;East China Univ Sci & Technol;Otto von Guericke Univ
- 来源：VACCINE 2019
- 关键词：MDCK suspension High cell density Influenza A virus production Process intensification Semi-perfusion Cell culture-based vaccine manufacturing
- Identification of multiple sources of the acidic charge variants in an IgG1 monoclonal antibody被引量：35收藏
- 作者：Miao, Shiwei Xie, Panpan Zou, Mao Fan, Li Liu, Xuping Zhou, Yan Zhao, Liang Ding, Ding Wang, Haibin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2017
- 关键词：mAb IgG1 Acidic charge heterogeneity Deamidation Galactosylation Disulfide bond reduction
- Enhancing Immune Response and Heterosubtypic Protection Ability of Inactivated H7N9 Vaccine by Using STING Agonist as a Mucosal Adjuvant被引量：35收藏
- 作者：Luo, Jian Liu, Xu-ping Xiong, Fei-fei Gao, Fei-xia Yi, Ying-lei Zhang, Min Chen, Ze Tan, Wen-song
- 机构：East China Univ Sci & Technol;Shanghai Inst Biol Prod
- 来源：FRONTIERS IN IMMUNOLOGY 2019
- 关键词：H7N9 whole viron vaccine STING mucosal adjuvant cross protection
- Elucidating the effects of pH shift on IgG1 monoclonal antibody acidic charge variant levels in Chinese hamster ovary cell cultures被引量：33收藏
- 作者：Xie, Panpan Niu, Huijie Chen, Xinning Zhang, Xintao Miao, Shiwei Deng, Xiancun Liu, Xuping Tan, Wen-Song Zhou, Yan Fan, Li
- 机构：East China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Acidic charge variants IgG1 monoclonal antibody Disulfide bond reduction Galactosylation Deamination Liquid chromatography mass spectrometry
- Galactose supplementation enhance sialylation of recombinant Fc-fusion protein in CHO cell: an insight into the role of galactosylation in sialylation被引量：31收藏
- 作者：Liu, Jintao Wang, Jie Fan, Li Chen, Xinning Hu, Dongdong Deng, Xiancun Poon, H. Fai Wang, Haibin Liu, Xuping Tan, Wen-Song
- 机构：E China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Hangzhou Co Ltd
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2015
- 关键词：Fc-fusion protein CHO cell Glycosylation dSialylation Galactose Galactosylation
- Detailed understanding of enhanced specific productivity in Chinese hamster ovary cells at low culture temperature被引量：26收藏
- 作者：Kou, Tian-Ci Fan, Li Zhou, Yan Ye, Zhao-Yang Liu, Xu-Ping Zhao, Liang Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2011
- 关键词：Chinese hamster ovary (CHO) cells TNFR-Fc mRNA stability Transcriptional rate Low temperature
- Culture temperature modulates monoclonal antibody charge variation distribution in Chinese hamster ovary cell cultures被引量：20收藏
- 作者：Zhang, Xintao Sun, Ya-Ting Tang, Hongping Fan, Li Hu, Dongdong Liu, Jintao Liu, Xuping Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2015
- 关键词：Basic carboxypeptidase Charge variation Chinese hamster ovary cells Culture temperature Lysine variation Monoclonal ntibodies Weak cation exchange chromatography
- Investigation into the impact of tyrosine on the product formation and quality attributes of mAbs in rCHO cell cultures被引量：16收藏
- 作者：Zhang, Weijian Liu, Xuping Tang, Hongping Zhang, Xinran Zhou, Yanan Fan, Li Wang, Haibin Tan, Wen-Song Zhao, Liang
- 机构：East China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2020
- 关键词：Chinese hamster ovary cells Tyrosine Fed-batch cultures Critical quality attribute CD20 Monoclonal antibody
- Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose被引量：15收藏
- 作者：Sun, Ya-ting Zhao, Liang Ye, Zhaoyang Fan, Li Liu, Xu-ping Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2013
- 关键词：CHO cells Fed-batch culture Antibody production Lactate metabolism Galactose Process development
- Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development被引量：12收藏
- 作者：Liu, Yaya Zhang, Weiyan Deng, Xiancun Poon, Hong Fai Liu, Xuping Tan, Wen-Song Zhou, Yan Fan, Li
- 机构：E China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2015
- 关键词：Basal medium design Chemical-defined Chinese hamster ovary High-yielding Monoclonal antibody
- A novel method based on nonparametric regression with a Gaussian kernel algorithm identifies the critical components in CHO media and feed optimization被引量：11收藏
- 作者：Zou, Mao Zhou, Zi-Wei Fan, Li Zhang, Wei-Jian Zhao, Liang Liu, Xu-Ping Wang, Hai-Bin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Shanghai Bioengine Scitech Co Ltd;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2020
- 关键词：Variable selection Nonparametric regression with Gaussian kernel Chinese hamster ovary cells Medium optimization
- Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protein production in Chinese hamster ovary cell culture被引量：11收藏
- 作者：Hu, Dongdong Sun, Yating Liu, Xuping Liu, Jintao Zhang, Xintao Zhao, Liang Wang, Haibin Tan, Wen-Song Fan, Li
- 机构：E China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Chinese hamster ovary cells Yeast extract Fc-fusion protein Specific productivity Cell cycle arrest mTOR
- Elucidating the effects of arginine and lysine on a monoclonal antibody C-terminal lysine variation in CHO cell cultures被引量：10收藏
- 作者：Zhang, Xintao Tang, Hongping Sun, Ya-Ting Liu, Xuping Tan, Wen-Song Fan, Li
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Chinese hamster ovary cells Monoclonal antibody Lysine variant Arginine Lysine Carboxypeptidase
- Pathway-pathway network-based study of the therapeutic mechanisms by which salvianolic acid B regulates cardiovascular diseases被引量：9收藏
- 作者：Ye Li He Yuan Ye Hao Liu XuePing Yang LinLin Cao ZhiWei Tang KaiLin
- 机构：Tongji Univ;E China Univ Sci & Technol;Shanghai Ctr Bioinformat Technol;Tongji Univ
- 来源：CHINESE SCIENCE BULLETIN 2012
- 关键词：cardiovascular diseases salvianolic acid B network analysis biological pathway network module analysis
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- Theoretical study of zeatin - A plant hormone and potential drug for neural diseases - On the basis of DFT, MP2 and target docking被引量：8收藏
- 作者：Liu, Xueping Berezniak, Tomasz Panek, Jaroslaw Jan Jezierska-Mazzarello, Aneta
- 机构：E China Univ Sci & Technol;Univ Wroclaw
- 来源：CHEMICAL PHYSICS LETTERS 2013
- Insights into the generation of monoclonal antibody acidic charge variants during Chinese hamster ovary cell cultures被引量：8收藏
- 作者：Tang, Hongping Miao, Shiwei Zhang, Xintao Fan, Li Liu, Xuping Tan, Wen-Song Zhao, Liang
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2018
- 关键词：Chinese hamster ovary cells Monoclonal antibody Charge heterogeneity Acidic variants Asparagine deamidation
- Uridine modulates monoclonal antibody charge heterogeneity in Chinese hamster ovary cell fed-batch cultures被引量：7收藏
- 作者：Niu, Huijie Wang, Jiaqi Liu, Mengjuan Chai, Miaomiao Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Charge heterogeneity Chinese hamster ovary cells Medium components Monoclonal antibody Uridine
- Highly efficient production of an influenza H9N2 vaccine using MDCK suspension cells被引量：7收藏
- 作者：Wu, Yixiao Jia, Hanjing Lai, Hanzhang Liu, Xuping Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Guangdong Wens Dahuanong Biotechnol Co Ltd;Zhaoqing Dahuanong Biol Med Co Ltd;Shanghai BioEngine Sci Tech Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2020
- 关键词：MDCK suspension cells Cell adaptation Influenza virus Feed strategy Cell culture-based vaccine manufacturing
- Characterization and minimization of sialic acid degradation in an Fc-fusion protein-producing CHO cell bioprocess被引量：6收藏
- 作者：Chen, Xinning Liu, Jintao Liu, Xuping Fan, Li Zhao, Liang Tan, Wen-Song
- 机构：East China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2018
- 关键词：Chinese hamster ovary cells Fc-fusion protein Sialic acid Sialidase pH
- Rational design of medium supplementation strategy for improved influenza viruses production based on analyzing nutritional requirements of MDCK Cells被引量：6收藏
- 作者：Huang, Ding Kang Xia-Hou Liu, Xu-Ping Zhao, Liang Fan, Li Ye, Zhaoyang Tan, Wen-Song Luo, Jian Chen, Ze
- 机构：E China Univ Sci & Technol;Shanghai Inst Biol Prod Co Ltd
- 来源：VACCINE 2014
- 关键词：Influenza virus MDCK cells Metabolism Nutritional requirements
- Biphasic addition strategy of hypoxanthine and thymidine for improving monoclonal antibody production被引量：6收藏
- 作者：Chen, Fei Ye, Zhaoyang Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2012
- 关键词：Chinese hamster ovary cells Hypoxanthine Thymidine Monoclonal antibody production Fed-batch culture
- Correlation of antibody production rate with glucose and lactate metabolism in Chinese hamster ovary cells被引量：5收藏
- 作者：Chen, Fei Ye, Zhaoyang Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2012
- 关键词：Antibody production Cell metabolism Chinese hamster ovary cells Mitochondrial dehydrogenase activity
- Insights into the loss of protein sialylation in an fc-fusion protein-producing CHO cell bioprocess被引量：5收藏
- 作者：Chen, Xinning Liu, Xuping Xiao, Zheng Liu, Jintao Zhao, Liang Tan, Wen-Song Fan, Li
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2019
- 关键词：Chinese hamster ovary cells Fc-fusion protein Sialylation Extracellular degradation Intracellular biosynthesis
- Physiological responses of Chinese hamster ovary cells to a productivity-enhancing yeast extract被引量：5收藏
- 作者：Hu, Dongdong Zhao, Liang Wang, Jiaqi Fan, Li Liu, Xuping Wang, Haibin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2018
- 关键词：Chinese hamster ovary cells Yeast extract Fc-fusion protein Specific productivity Amino acid metabolism Energy charge
- Application of improved top-down approach in maximizing CHO cell mass and productivity in fed-batch culture被引量：5收藏
- 作者：Sun, Ya-Ting Zhao, Liang Liu, Xu-Ping Hu, Dong-dong Fan, Li Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 2013
- 关键词：CHO cells top-down approach fed-batch culture monoclonal antibody amino acids
- Process analysis of reduced specific productivity of TNFR-Fc in Chinese hamster ovary cells at high cell density被引量：5收藏
- 作者：Kou, Tian-Ci Fan, Li Ye, Zhao-Yang Zhou, Yan Liu, Xu-Ping Zhao, Liang Tan, Wen-Song
- 机构：E China Univ Chem Technol
- 来源：PROCESS BIOCHEMISTRY 2011
- 关键词：Cell density CHO cells TNFR-Fc Specific productivity Process analysis
- Effects of Calcium Ion on Adenovirus Production with High Densities of HEK293 Cells被引量：3收藏
- 作者：Liu, Xuping Zhao, Liang Wang, Yigang Zhang, Xu Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 2010
- 关键词：adenovirus calcium ion cell density HEK293 cells virus production
