成果/Result
- Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris被引量：136收藏
- 作者：Wang, Jinjia Wang, Xiaolong Shi, Lei Qi, Fei Zhang, Ping Zhang, Yuanxing Zhou, Xiangshan Song, Zhiwei Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;Bioproc Technol Inst;Natl Univ Singapore
- 来源：SCIENTIFIC REPORTS 2017
- Genetic, molecular, and biochemical basis of fungal tropolone biosynthesis被引量：133收藏
- 作者：Davison, Jack al Fahad, Ahmed Cai, Menghao Song, Zhongshu Yehia, Samar Y. Lazarus, Colin M. Bailey, Andrew M. Simpson, Thomas J. Cox, Russell J.
- 机构：Univ Bristol;E China Univ Sci & Technol;Future Univ Egypt;Univ Bristol
- 来源：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2012
- 关键词：oxidative rearrangement azaphilone colchicine
- Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol被引量：123收藏
- 作者：Liu, Yiqi Tu, Xiaohu Xu, Qin Bai, Chenxiao Kong, Chuixing Liu, Qi Yu, Jiahui Peng, Qiangqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Methanol Methylotrophic yeast Co-culture Heterologous biosynthesis Monacolin J Lovastatin
- Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris被引量：95收藏
- 作者：Wang, Xiaolong Wang, Qi Wang, Jinjia Bai, Peng Shi, Lei Shen, Wei Zhou, Mian Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：E China Univ Sci & Technol;SCICB
- 来源：JOURNAL OF BIOLOGICAL CHEMISTRY 2016
- 关键词：DNA-protein interaction protein domain signal transduction transcription coactivator transcription regulation AOX1 promoter Mit1 Pichia pastoris glycerol repression methanol induction
- CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris被引量：89收藏
- 作者：Liu, Qi Shi, Xiaona Song, Lili Liu, Haifeng Zhou, Xiangshan Wang, Qiyao Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Chinare Resources Angde Biotech Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：MICROBIAL CELL FACTORIES 2019
- 关键词：Pichia pastoris CRISPR-Cas9 Homology directed repair Multiloci integration Multistep enzymatic pathway
- A novel methanol-free Pichia pastoris system for recombinant protein expression被引量：60收藏
- 作者：Shen, Wei Xue, Ying Liu, Yiqi Kong, Chuixing Wang, Xiaolong Huang, Mengmeng Cai, Menghao Zhou, Xiangshan Zhang, Yuanxing Zhou, Mian
- 机构：East China Univ Sci & Technol;SCICB
- 来源：MICROBIAL CELL FACTORIES 2016
- 关键词：Recombination protein expression AOX1 promoter Dihydroxyacetone GUT1 DAK Pichia pastoris
- Structure and physicochemical properties of amphiphilic agar modified with octenyl succinic anhydride被引量：56收藏
- 作者：Chen, Huijing Chen, Fuquan Xiao, Qiong Cai, Menghao Yang, Qiuming Weng, Huifen Xiao, Anfeng
- 机构：Jimei Univ;East China Univ Sci & Technol;Natl R&D Ctr Red Alga Proc Technol;Fujian Prov Engn Technol Res Ctr Marine Funct Foo
- 来源：CARBOHYDRATE POLYMERS 2021
- 关键词：Agar Octenyl succinic anhydride Characterization Rheology
- Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production被引量：52收藏
- 作者：Gao, Limei Cai, Menghao Shen, Wei Xiao, Siwei Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2013
- 关键词：Polyketide Pichia pastoris 6-MSA 6-MSAS Heterologous expression Fermentation
- Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast被引量：45收藏
- 作者：Liu, Yiqi Bai, Chenxiao Liu, Qi Xu, Qin Qian, Zhilan Peng, Qiangqiang Yu, Jiahui Xu, Mingqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Ethanol Crabtree-negative yeast Acetyl-CoA Monacolin J Metabolic engineering Coculture
- Characterization of a thermostable raw-starch hydrolyzing α-amylase from deep-sea thermophile Geobacillus sp.被引量：43收藏
- 作者：Jiang, Tao Cai, Menghao Huang, Mengmeng He, Hao Lu, Jian Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2015
- 关键词：alpha-Amylase Geobacillus sp. Heterologous expression Thermophile
- Regulating unfolded protein response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris被引量：39收藏
- 作者：Huang, Mengmeng Gao, Yanyun Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Unfolded protein response HAC1p Heterologous expression P. pastoris Raw-starch hydrolyzing alpha-amylase
- PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris被引量：38收藏
- 作者：Wang, Xiaolong Cai, Menghao Shi, Lei Wang, Qi Zhu, Jinxiang Wang, Jinjia Zhou, Mian Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY LETTERS 2016
- 关键词：AOX1 promoter Catabolite repression Glucose Glycerol PpNrg1 Pichia pastoris Transcriptional regulation
- Identification and characterization of novel promoters for recombinant protein production in yeast Pichia pastoris被引量：37收藏
- 作者：Xu, Ning Zhu, Jinxiang Zhu, Qiaoyun Xing, Yanzi Cai, Menghao Jiang, Tianyi Zhou, Mian Zhang, Yuanxing
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;Roche R&D Ctr China Ltd
- 来源：YEAST 2018
- 关键词：carbon source Pichia pastoris promoter recombinant protein expression
- Genetically shaping morphology of the filamentous fungus Aspergillus glaucus for production of antitumor polyketide aspergiolide A被引量：37收藏
- 作者：Cai, Menghao Zhang, Ying Hu, Wei Shen, Wei Yu, Zhenzhong Zhou, Weiqiang Jiang, Tao Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2014
- 关键词：Filamentous fungi Aspergillus glaucus Genetically morphology shaping Shear stress Aspergiolide A
- Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway被引量：36收藏
- 作者：Xue, Ying Kong, Chuixing Shen, Wei Bai, Chenxiao Ren, Yanna Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Polyketides Pichia pastoris Heterologous biosynthesis Citrinin Biosynthetic pathway
- A programmable high-expression yeast platform responsive to user-defined signals被引量：35收藏
- 作者：Liu, Qi Song, Lili Peng, Qiangqiang Zhu, Qiaoyun Shi, Xiaona Xu, Mingqiang Wang, Qiyao Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SCIENCE ADVANCES 2022
- 关键词：Recombinant proteins - Transcription
- Preparation, characterization, and application of high-whiteness agar bleached with hydrogen peroxide被引量：35收藏
- 作者：An, Ding Xiao, Qiong Zhang, Cong Cai, Menghao Zhang, Yonghui Weng, Huifen Chen, Fuquan Xiao, Anfeng
- 机构：Jimei Univ;East China Univ Sci & Technol;Natl R&D Ctr Red Alga Proc Technol;Fujian Prov Engn Technol Res Ctr Marine Funct Foo
- 来源：FOOD HYDROCOLLOIDS 2021
- 关键词：High-whiteness agar Hydrogen peroxide Bleaching Quality promotion Diversified application
- De Novo Production of Plant 4′-Deoxyflavones Baicalein and Oroxylin A from Ethanol in Crabtree-Negative Yeast被引量：32收藏
- 作者：Qian, Zhilan Yu, Jiahui Chen, Xinjie Kang, Yijia Ren, Yanna Liu, Qi Lu, Jian Zhao, Qing Cai, Menghao
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Chinese Acad Sci;East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：plant 4 '-deoxyflavones Scutellaria baicalensis Georgi Pichia pastoris transcriptional regulation device fine-tuning pathway control
- Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus被引量：32收藏
- 作者：Cai, Meng-Hao Zhou, Xiang-Shan Sun, Xue-Qian Tao, Ke-Jing Zhang, Yuan-Xing
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2009
- 关键词：Aspergillus glaucus Aspergiolide A Statistical methodologies Medium optimization Carbon-nitrogen ratio
- Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism被引量：30收藏
- 作者：Xu, Qin Bai, Chenxiao Liu, Yiqi Song, Lili Tian, Lin Yan, Yunfeng Zhou, Jinfeng Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY FOR BIOFUELS 2019
- 关键词：Komagataella phaffii Acetate utilization Acetyl-CoA Kinase screening Metabolic engineering
- Polyketides in Aspergillus terreus: biosynthesis pathway discovery and application被引量：29收藏
- 作者：Yin, Ying Cai, Menghao Zhou, Xiangshan Li, Zhiyong Zhang, Yuanxing
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Polyketide Aspergillus terreus Biosynthesis pathway Gene cluster Heterologous expression
- Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis被引量：26收藏
- 作者：Kong, Chuixing Huang, Hezhou Xue, Ying Liu, Yiqi Peng, Qiangqiang Liu, Qi Xu, Qin Zhu, Qiaoyun Yin, Ying Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SCIENTIFIC REPORTS 2018
- Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously被引量：24收藏
- 作者：Sun, Xueqian Zhou, Xiangshan Cai, Menghao Tao, Kejing Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2009
- 关键词：Aspergillus glaucus Aspergiolide A Pathway specific inhibitor Polyketide pathway Precursor
- Genome-Wide Determination of Gene Essentiality by Transposon Insertion Sequencing in Yeast Pichia pastoris被引量：23收藏
- 作者：Zhu, Jinxiang Gong, Ruiqing Zhu, Qiaoyun He, Qiulin Xu, Ning Xu, Yichun Cai, Menghao Zhou, Xiangshan Zhang, Yuanxing Zhou, Mian
- 机构：East China Univ Sci & Technol;SCICB
- 来源：SCIENTIFIC REPORTS 2018
- Kinase Screening in Pichia pastoris Identified Promising Targets Involved in Cell Growth and Alcohol Oxidase 1 Promoter (PAOX1) Regulation被引量：22收藏
- 作者：Shen, Wei Kong, Chuixing Xue, Ying Liu, Yiqi Cai, Menghao Zhang, Yuanxing Jiang, Tianyi Zhou, Xiangshan Zhou, Mian
- 机构：East China Univ Sci & Technol;SCICB;Roche R&D Ctr China Ltd
- 来源：PLOS ONE 2016
- Producing Novel Fibrinolytic Isoindolinone Derivatives in Marine Fungus Stachybotrys longispora FG216 by the Rational Supply of Amino Compounds According to Its Biosynthesis Pathway被引量：22收藏
- 作者：Yin, Ying Fu, Qiang Wu, Wenhui Cai, Menghao Zhou, Xiangshan Zhang, Yuanxing
- 机构：East China Univ Sci & Technol;Shanghai Ocean Univ;Shanghai Collaborat Innovat Ctr Biomfg;Chinese Acad Sci
- 来源：MARINE DRUGS 2017
- 关键词：Stachybotrys isoindolinone biosynthesis genome mining amino compound fibrinolytic activity
- Biosynthesis of aspergiolide A, a novel antitumor compound by a marine-derived fungus Aspergillus glaucus via the polyketide pathway被引量：22收藏
- 作者：Tao, Kejing Du, Lin Sun, Xueqian Cai, Menghao Zhu, Tianjiao Zhou, Xiangshan Gu, Qianqun Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Ocean Univ China
- 来源：TETRAHEDRON LETTERS 2009
- 关键词：Aspergillus glaucus Biosynthesis Polyketide pathway C-13 NMR Feeding experiments Natural products
- Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor被引量：21收藏
- 作者：Cai, Menghao Zhou, Xiangshan Lu, Jian Fan, Weimin Niu, Chuanpeng Zhou, Jiushun Sun, Xueqian Kang, Li Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2011
- 关键词：Aspergillus glaucus Aspergiolide A Oxygen carrier Shear-sensitive
- A Synthetic Malonyl-CoA Metabolic OSCIllator in Komagataella phaffii被引量：21收藏
- 作者：Wen, Jiao Tian, Lin Xu, Mingqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：ACS SYNTHETIC BIOLOGY 2020
- Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges被引量：19收藏
- 作者：Lv, Wen Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：FEMS YEAST RESEARCH 2025
- 关键词：Komagataella phaffii non-conventional yeast protein expression regulation advanced methods biotechnology applications
