成果/Result
- Expression and Characterization of a Novel Lipase from Aspergillus fumigatus with High Specific Activity被引量：31收藏
- 作者：Shangguan, Jiao-Jiao Liu, Yu-Qiang Wang, Fu-Jun Zhao, Jian Fan, Li-Qiang Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Aspergillus fumigatus Recombinant lipase Expression Characterization Active site
- caspase-mediated LPS sensing and pyroptosis signaling in Hydra被引量：31收藏
- 作者：Chen, Shouwen Li, Shuxin Chen, Hao Gong, Yuxin Yang, Dahai Zhang, Yuanxing Liu, Qin
- 机构：East China Univ Sci & Technol;Qingdao Natl Lab Marine Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai
- 来源：SCIENCE ADVANCES 2023
- 关键词：Network security
- Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol被引量：29收藏
- 作者：Zhu, Qing-Qing He, Wan-Hong Kong, Xu-Dong Fan, Li-Qiang Zhao, Jian Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：RT-PCR Vigna radiata epoxide hydrolase p-Nitrostyrene oxide Enantioconvergency Characterization
- Dual function of a turbot inflammatory caspase in mediating both canonical and non-canonical inflammasome activation被引量：28收藏
- 作者：Chen, Shouwen Jin, Peng Chen, Hao Wu, Di Li, Shuxin Zhang, Yuanxing Liu, Qin Yang, Dahai
- 机构：East China Univ Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai;Shanghai Engn Res Ctr Marine Cultured Anim Vaccin
- 来源：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 2021
- 关键词：Turbot Inflammatory caspase Cytosolic LPS receptor NLRP3 inflammasome GSDME Pyroptosis Antibacterial activity
- A novel human derived cell-penetrating peptide in drug delivery被引量：20收藏
- 作者：Zhao, Jian Gao, Peng Xiao, Wei Fan, Li-Qiang Wang, Fu-Jun Li, Su-Xia Liu, Jian-Wen
- 机构：E China Univ Sci & Technol;Zhejiang Reachall Pharmaceut Co Ltd
- 来源：MOLECULAR BIOLOGY REPORTS 2011
- 关键词：hC-SOD3 Human cell-penetrating peptide Apoptin Drug delivery
- Production, Characterization, and Application of an Organic Solvent-Tolerant Lipase Present in Active Inclusion Bodies被引量：16收藏
- 作者：Li, Suxia Lin, Kang Pang, Huaiyu Wu, Yixin Xu, Jianhe
- 机构：E China Univ Sci & Technol;Hamamatsu Univ Sch Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2013
- 关键词：Lipase Active inclusion body Organic solvent tolerance (+/-)-MPGM Serratia marcescens ECU1010
- Significantly Improved Expression and Biochemical Properties of Recombinant Serratia marcescens Lipase as Robust Biocatalyst for Kinetic Resolution of Chiral Ester被引量：16收藏
- 作者：Wang, Yi Zhao, Jian Xu, Jian-He Fan, Li-Qiang Li, Su-Xia Zhao, Li-Li Mao, Xiao-Bo
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Serratia marcescens ECU1010 Lipase Soluble expression Purification Characterization Enantioselectivity Biocatalytic resolution
- Refolding, purification and characterization of an organic solvent-tolerant lipase from Serratia marcescens ECU1010被引量：10收藏
- 作者：Li, Suxia Pang, Huaiyu Lin, Kang Xu, Jianhe Zhao, Jian Fan, Liqiang
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：Lipase Serratia marcescens Refolding Organic solvent tolerance FT-IR microspectroscopy
- Preparation and Irreversible Inhibition Mechanism Insight into a Recombinant Kunitz Trypsin Inhibitor from Glycine max L. Seeds被引量：9收藏
- 作者：Xu, Yanji Zhang, Panpan Liu, Xiao Wang, Zhike Li, Suxia
- 机构：East China Univ Sci & Technol;Shanghai Yaxin Biotechnol Ltd Co
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2020
- 关键词：Soybean Kunitz trypsin inhibitor Protein refolding Biochemical property Inhibition kinetic assay Molecular modeling
- PCR-RFLP to Detect Codon 248 Mutation in Exon 7 of p53 Tumor Suppressor Gene被引量：8收藏
- 作者：Ouyang, Liming Ge, Chongtao Wu, Haizhen Li, Suxia Zhang, Huizhan
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION 2009
- 关键词：PCR-RFLP p53 gene codon 248 mutation genomics experiment
- Cloning and expression of a new rat procarboxypeptidase B gene in Escherichia coli and purification of recombination carboxypeptidase B被引量：7收藏
- 作者：Li, SX Zhang, YJ Tian, LP Yuan, QS Gong, Y
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：PROTEIN AND PEPTIDE LETTERS 2003
- 关键词：rat procarboxypeptidase B RT-PCR recombinant carboxypeptidase B cloning and expression specific activity purification application
- The renaturation of procarboxypeptidase B by urea gradient gel filtration and some properties of recombinant carboxypeptidase B被引量：6收藏
- 作者：Zhang, XY Li, SX Yuan, QS
- 机构：E China Univ Sci & Technol
- 来源：PROTEIN AND PEPTIDE LETTERS 2005
- 关键词：procarboxypeptidase B recombinant carboxypeptidase B urea gradient gel filtration renaturation dilution refolding properties
- Essential Role of Gly33 in a Novel Organic Solvent-Tolerant Lipase from Serratia marcescens ECU1010 as Determined by Site-Directed Mutagenesis被引量：5收藏
- 作者：Li, Su-Xia Ma, Qiang Lin, Kang Wu, Jiao-Jiao Wu, Yi-Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol;Hamamatsu Univ Sch Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2014
- 关键词：Lipase Site-directed mutagenesis Organic solvent stability Molecular modeling Serratia marcescens ECU1010
- Hydra gasdermin-gated pyroptosis signalling regulates tissue regeneration被引量：5收藏
- 作者：Chen, Shouwen Gong, Yuxin Li, Shuxin Yang, Dahai Zhang, Yuanxing Liu, Qin
- 机构：East China Univ Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai;Shanghai Engn Res Ctr Marine Cultured Anim Vaccine
- 来源：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 2023
- 关键词：Gasdermin Pyroptosis Wnt Mitochondrial ROS Regeneration
- Identification and characterization of an androgen-responsive Kap promoter enhancer located in the intron II region of human angiotensinogen gene被引量：4收藏
- 作者：Fan, Li-qiang Hardy, Dianne O. Catterall, James F. Zhao, Jian Li, Su-xia
- 机构：E China Univ Sci & Technol;Populat Council
- 来源：JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 2010
- 关键词：Androgen Glucocorticoid Enhancer Gene regulation hAGT Kap promoter
- A Biochemistry and Molecular Biology Experiment and Evaluation System for Biotechnology Specialty Students AN EFFECTIVE EVALUATION SYSTEM TO IMPROVE THE BIOCHEMISTRY AND MOLECULAR BIOLOGY EXPERIMENT TEACHING被引量：3收藏
- 作者：Li, Suxia Wu, Haizhen Zhao, Jian Ou, Ling Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION 2010
- 关键词：Biochemistry and molecular biology education evaluation system experimental presentation experiment teaching
- Increasing the refolding efficiency in vitro by site-directed mutagenesis of Cys383 in rat procarboxypeptidase B被引量：2收藏
- 作者：Li, Suxia Zhang, Luosheng Wu, Qian Xin, Aijie Zhao, Jian Fan, Liqiang
- 机构：E China Univ Sci & Technol
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2011
- 关键词：Procarboxypeptidase B Mutation Cysteine Refolding efficiency Property
- Effect of K225 residue to the catalytic efficiency of Kex2 protease被引量：2收藏
- 作者：Yang, Fan Liu, Li Liu, Yingying Li, Suxia
- 机构：East China Univ Sci & Technol
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2020
- 关键词：Kex2 protease Pichia pastoris Kex2-K225L mutant Stability Steady-state kinetics
- The Small GTPase Activity of the ROC Domain from LRRK2, a Parkinson's Disease Related Protein被引量：2收藏
- 作者：Fu, Qing-Shan Song, Ai-Xin Li, Su-Xia Hu, Hong-Yu
- 机构：Chinese Acad Sci;E China Univ Sci & Technol
- 来源：PROTEIN AND PEPTIDE LETTERS 2009
- 关键词：LRRK2 ROC domain small GTPase GTP binding Parkinson's disease
- Identification and characterization of the minimal androgen-regulated kidney-specific kidney androgen-regulated protein gene promoter被引量：1收藏
- 作者：Fan, Liqiang Hardy, Dianne O. Catteral, James F. Zhao, Jian Li, Suxia
- 机构：E China Univ Sci & Technol;Populat Council
- 来源：ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2008
- 关键词：androgen receptor kidney proximal tubule cell promoter
- Expression of C-peptide multiple gene copies in Escherichia coli and stabilities of C-peptide in aqueous solution被引量：1收藏
- 作者：Li, SX Tian, LP Liu, HF Zhang, YJ Hu, XB Gong, Y Yuan, QS
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：ACTA BIOCHIMICA ET BIOPHYSICA SINICA 2003
- 关键词：proinsulin C-peptide multiple copies expression stability RP-HPLC
- Preparation and application of Taq DNA polymerase monoclonal antibody guided by structural domain analysis被引量：0收藏
- 作者：Zhu, Xiaoqian Zhang, Tingting Zhao, Haiyu Hou, Bingbing Fu, Mingyue Zhao, Qianshan Xu, Tong Zhao, Jixiang Xie, Jiming Zhou, Yuyao Li, Suxia Wang, Peng
- 机构：East China Univ Sci & Technol;Shanghai Yaxin Biotechnol Co Ltd
- 来源：PLOS ONE 2026
- Cleavage of gasdermin by apoptotic caspases triggers pyroptosis restricting bacterial colonization in Hydra被引量：0收藏
- 作者：Li, Shuxin Lin, Xiuqing Zhang, Yuanxing Liu, Qin Chen, Shouwen
- 机构：East China Univ Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai;Shanghai Engn Res Ctr Marine Cultured Anim Vaccine
- 来源：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 2024
- 关键词：Gasdermin GSDME Evolution Recognition motif Hydra
