成果/Result
- Understanding the mechanistic roles of 30Kc6 gene in apoptosis and specific productivity in antibody-producing Chinese hamster ovary cells被引量：36收藏
- 作者：Wang, Zesong Ma, Xuhui Fan, Li Rhee, Won Jong Park, Tai Hyun Zhao, Liang Tan, Wen-Song
- 机构： State Key Laboratory of Bioreactor Engineering; Biopharmaceutical R and D; School of Chemical and Biological Engineering
- 来源：Applied Microbiology and Biotechnology 2012
- 关键词：Antibiotics - Productivity - Amino acids - Cell membranes - Mammals - Recombinant protEIns - Mitochondria - Monoclonal antibodies - Transcription - Cell culture - Chemical activation
- Identification of multiple sources of the acidic charge variants in an IgG1 monoclonal antibody被引量：35收藏
- 作者：Miao, Shiwei Xie, Panpan Zou, Mao Fan, Li Liu, Xuping Zhou, Yan Zhao, Liang Ding, Ding Wang, Haibin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2017
- 关键词：mAb IgG1 Acidic charge heterogenEIty Deamidation Galactosylation Disulfide bond reduction
- Expression of anti-apoptotic 30Kc6 gene inhibiting hyperosmotic pressure-induced apoptosis in antibody-producing Chinese hamster ovary cells被引量：34收藏
- 作者：Wang, Zesong Ma, Xuhui Zhao, Liang Fan, Li Tan, Wen-Song
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2012
- 关键词：Cell culture - Mitochondria - Cell membranes - Recombinant protEIns - Osmosis - Productivity - Antibodies - Gene expression - Mammals
- Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media: cell growth, antibody production, metabolic waste, glutamate, and energy metabolism被引量：34收藏
- 作者：Zhang, Li-xiang Zhang, Wei-yan Wang, Chen Liu, Jin-tao Deng, Xian-cun Liu, Xu-ping Fan, Li Tan, Wen-song
- 机构： The State Key Laboratory of Bioreactor Engineering; Zhejiang Hisun Pharmaceutical Co. Ltd
- 来源：Bioresources and Bioprocessing 2016
- 关键词：Amino acids - Cell culture - Cell growth - Growth kinetics - Monoclonal antibodies - Physiology
- Elucidating the effects of pH shift on IgG1 monoclonal antibody acidic charge variant levels in Chinese hamster ovary cell cultures被引量：33收藏
- 作者：Xie, Panpan Niu, Huijie Chen, Xinning Zhang, Xintao Miao, Shiwei Deng, Xiancun Liu, Xuping Tan, Wen-Song Zhou, Yan Fan, Li
- 机构：East China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Acidic charge variants IgG1 monoclonal antibody Disulfide bond reduction Galactosylation Deamination Liquid chromatography mass spectrometry
- The combined effect of sodium butyrate and low culture temperature on the production, sialylation, and biological activity of an antibody produced in CHO cells被引量：33收藏
- 作者：Chen, Fei Kou, Tianci Fan, Li Zhou, Yan Ye, Zhaoyang Zhao, Liang Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 2011
- 关键词：CHO cells sodium butyrate low culture temperature sialylation N-glycolylneuraminic acid
- Galactose supplementation enhance sialylation of recombinant Fc-fusion protEIn in CHO cell: an insight into the role of galactosylation in sialylation被引量：31收藏
- 作者：Liu, Jintao Wang, Jie Fan, Li Chen, Xinning Hu, Dongdong Deng, Xiancun Poon, H. Fai Wang, Haibin Liu, Xuping Tan, Wen-Song
- 机构：E China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Hangzhou Co Ltd
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2015
- 关键词：Fc-fusion protEIn CHO cell Glycosylation dSialylation Galactose Galactosylation
- Detailed understanding of enhanced specific productivity in Chinese hamster ovary cells at low culture temperature被引量：26收藏
- 作者：Kou, Tian-Ci Fan, Li Zhou, Yan Ye, Zhao-Yang Liu, Xu-Ping Zhao, Liang Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2011
- 关键词：Chinese hamster ovary (CHO) cells TNFR-Fc mRNA stability Transcriptional rate Low temperature
- Culture temperature modulates monoclonal antibody charge variation distribution in Chinese hamster ovary cell cultures被引量：20收藏
- 作者：Zhang, Xintao Sun, Ya-Ting Tang, Hongping Fan, Li Hu, Dongdong Liu, Jintao Liu, Xuping Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2015
- 关键词：Basic carboxypeptidase Charge variation Chinese hamster ovary cells Culture temperature Lysine variation Monoclonal ntibodies Weak cation exchange chromatography
- Insight into the roles of hypoxanthine and thydimine on cultivating antibody-producing CHO cells: cell growth, antibody production and long-term stability被引量：18收藏
- 作者：Chen, Fei Fan, Li Wang, Jiaqi Zhou, Yan Ye, Zhaoyang Zhao, Liang Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2012
- 关键词：Chinese hamster ovary cells Hypoxanthine Thymidine Methotrexate Long-term stability Monoclonal antibody
- Investigation into the impact of tyrosine on the product formation and quality attributes of mAbs in rCHO cell cultures被引量：16收藏
- 作者：Zhang, Weijian Liu, Xuping Tang, Hongping Zhang, Xinran Zhou, Yanan Fan, Li Wang, Haibin Tan, Wen-Song Zhao, Liang
- 机构：East China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2020
- 关键词：Chinese hamster ovary cells Tyrosine Fed-batch cultures Critical quality attribute CD20 Monoclonal antibody
- Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose被引量：15收藏
- 作者：Sun, Ya-ting Zhao, Liang Ye, Zhaoyang Fan, Li Liu, Xu-ping Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2013
- 关键词：CHO cells Fed-batch culture Antibody production Lactate metabolism Galactose Process development
- MOI对HEK293细胞感染病毒后的生长代谢和腺病毒扩增效率的影响被引量：12收藏
- 作者：刘旭平 范里 朱明龙 张旭 谭文松
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《高校化学工程学报》 2010
- 关键词：HEK293细胞 腺病毒 病毒扩增效率 MOI
- 摘要：分别在方瓶贴壁和转瓶悬浮二种培养模式下,考察了感染复数(Multiplicity of Infection,MOI)对HEK293细胞感染病毒后的生长代谢和腺病毒扩增效率的影响。通过实验发现,感染病毒后的细胞跟未感染病毒...
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- 表达单克隆抗体的CHO细胞无蛋白培养基的优化被引量：12收藏
- 作者：刘国庆 陈飞 赵亮 范里 谭文松
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《高校化学工程学报》 2013
- 关键词：CHO细胞 单克隆抗体 无蛋白培养基 优化
- 摘要：在自主研发无蛋白培养基的基础上,考察了氨基酸、维生素和葡萄糖对CHO细胞生长、代谢与抗体合成的影响。结果表明,对培养过程中消耗较多的氨基酸进行补充,虽然不能促进细胞生长,但有利于培养后期细胞活性维持与抗体合成;B族维生素...
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- Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development被引量：12收藏
- 作者：Liu, Yaya Zhang, Weiyan Deng, Xiancun Poon, Hong Fai Liu, Xuping Tan, Wen-Song Zhou, Yan Fan, Li
- 机构：E China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2015
- 关键词：Basal medium design Chemical-defined Chinese hamster ovary High-yielding Monoclonal antibody
- A novel method based on nonparametric regression with a Gaussian kernel algorithm identifies the critical components in CHO media and feed optimization被引量：11收藏
- 作者：Zou, Mao Zhou, Zi-Wei Fan, Li Zhang, Wei-Jian Zhao, Liang Liu, Xu-Ping Wang, Hai-Bin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Shanghai Bioengine Scitech Co Ltd;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2020
- 关键词：Variable selection Nonparametric regression with Gaussian kernel Chinese hamster ovary cells Medium optimization
- Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protEIn production in Chinese hamster ovary cell culture被引量：11收藏
- 作者：Hu, Dongdong Sun, Yating Liu, Xuping Liu, Jintao Zhang, Xintao Zhao, Liang Wang, Haibin Tan, Wen-Song Fan, Li
- 机构：E China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Chinese hamster ovary cells Yeast extract Fc-fusion protEIn Specific productivity Cell cycle arrest mTOR
- Elucidating the effects of arginine and lysine on a monoclonal antibody C-terminal lysine variation in CHO cell cultures被引量：10收藏
- 作者：Zhang, Xintao Tang, Hongping Sun, Ya-Ting Liu, Xuping Tan, Wen-Song Fan, Li
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Chinese hamster ovary cells Monoclonal antibody Lysine variant Arginine Lysine Carboxypeptidase
- Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells被引量：10收藏
- 作者：Fan, Li Zhao, Liang Ye, Zhaoyang Sun, Yating Kou, Tianci Zhou, Yan Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2010
- 关键词：CHO cells Culture temperature Specific productivity TNFR-Fc
- Responses of GS-NS0 Myeloma Cells to Osmolality: Cell Growth, Intracellular Mass Metabolism, Energy Metabolism, and Antibody Production被引量：9收藏
- 作者：Zhao, Liang Fan, Li Wang, Jiaqi Niu, Hongxing Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 2009
- 关键词：GS-NS0 cells osmolality cell growth metabolism antibody production
- Insights into the generation of monoclonal antibody acidic charge variants during Chinese hamster ovary cell cultures被引量：8收藏
- 作者：Tang, Hongping Miao, Shiwei Zhang, Xintao Fan, Li Liu, Xuping Tan, Wen-Song Zhao, Liang
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2018
- 关键词：Chinese hamster ovary cells Monoclonal antibody Charge heterogenEIty Acidic variants Asparagine deamidation
- Increasing the Productivity of TNFR-Fc in GS-CHO Cells at Reduced Culture Temperatures被引量：8收藏
- 作者：Kou, Tian-Ci Fan, Li Zhou, Yan Ye, Zhao-Yang Zhao, Liang Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 2011
- 关键词：cell growth arrest GS-CHO cells low temperature TNFR-Fc transcriptional level
- Uridine modulates monoclonal antibody charge heterogenEIty in Chinese hamster ovary cell fed-batch cultures被引量：7收藏
- 作者：Niu, Huijie Wang, Jiaqi Liu, Mengjuan Chai, Miaomiao Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Charge heterogenEIty Chinese hamster ovary cells Medium components Monoclonal antibody Uridine
- Cell culture medium supplemented with taurine decreases basic charge variant levels of a monoclonal antibody被引量：7收藏
- 作者：Liu, Mengjuan Wang, Jiaqi Tang, Hongping Fan, Li Zhao, Liang Wang, Hai-Bin Zhou, Yan Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Shanghai Bioengine Biotechnol Co Ltd;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：BIOTECHNOLOGY LETTERS 2018
- 关键词：Basic charge variation Chinese hamster ovary cell Lysine variation Monoclonal antibody Oxidation Taurine
- Ultra-low carbon dioxide partial pressure improves the galactosylation of a monoclonal antibody produced in Chinese hamster ovary cells in a bioreactor被引量：7收藏
- 作者：Wang, Chen Wang, Jiaqi Chen, Min Fan, Li Zhao, Liang Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Shanghai Bioengine Biotechnol Co Ltd
- 来源：BIOTECHNOLOGY LETTERS 2018
- 关键词：Chinese hamster ovary cell Glycosylation pCO(2) Scale-up Monoclonal antibody
- Insight into the roles of tyrosine on rCHO cell performance in fed-batch cultures被引量：7收藏
- 作者：Tang, Hongping Zhang, Xintao Zhang, Weijian Fan, Li Wang, Haibin Tan, Wen-Song Zhao, Liang
- 机构：East China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2019
- 关键词：Chinese hamster ovary cells Tyrosine Cell death Autophagy Culture pH
- Characterization and minimization of sialic acid degradation in an Fc-fusion protEIn-producing CHO cell bioprocess被引量：6收藏
- 作者：Chen, Xinning Liu, Jintao Liu, Xuping Fan, Li Zhao, Liang Tan, Wen-Song
- 机构：East China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2018
- 关键词：Chinese hamster ovary cells Fc-fusion protEIn Sialic acid Sialidase pH
- Biphasic addition strategy of hypoxanthine and thymidine for improving monoclonal antibody production被引量：6收藏
- 作者：Chen, Fei Ye, Zhaoyang Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2012
- 关键词：Chinese hamster ovary cells Hypoxanthine Thymidine Monoclonal antibody production Fed-batch culture
- Correlation of antibody production rate with glucose and lactate metabolism in Chinese hamster ovary cells被引量：5收藏
- 作者：Chen, Fei Ye, Zhaoyang Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2012
- 关键词：Antibody production Cell metabolism Chinese hamster ovary cells Mitochondrial dehydrogenase activity
- 基于单纯型设计和部件搜索方法的CHO细胞无血清培养基的高通量优化被引量：5收藏
- 作者：李晓璐 范里 赵亮 陈飞 谭文松
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《高校化学工程学报》 2014
- 关键词：CHO细胞 无血清培养基 高通量筛选技术 氨基酸优化
- 摘要：氨基酸的浓度与配比对细胞生长和产物表达有重要作用。在自主研发无血清培养基的基础上,通过单纯型格子实验设计优化氨基酸浓度及配比后发现,优化的无血清培养基明显促进了细胞生长。生物反应器批式培养中,最高活细胞密度达52.6×1...
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