成果/Result
- Mutant library construction in directed molecular evolution: Casting a wider net被引量：74收藏
- 作者：Wang, Tian-Wen Zhu, Hu Ma, Xing-Yuan Zhang, Ting Ma, Yu-Shu Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Molecular Biotechnology 2006
- 关键词：Cells - DNA - Enzymes - Molecular biology - Mutagenesis - Mutagens
- Rhodamine 6G conjugated-quantum dots used for highly sensitive and selective ratiometric fluorescence sensor of glutathione被引量：62收藏
- 作者：Gui, Rijun An, Xueqin Su, Hongjuan Shen, Weiguo Zhu, Linyong Ma, Xingyuan Chen, Zhiyun Wang, Xiaoyong
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：TALANTA 2012
- 关键词：Quantum dots Fluorescence Glutathione Ratiometric sensor
- Development of Novel Lignin-Based Targeted Polymeric Nanoparticle Platform for Efficient Delivery of Anticancer Drugs被引量：51收藏
- 作者：Liu, Kefeng Zheng, Dan Lei, Hantian Liu, Jing Lei, Jiandu Wang, Luying Ma, Xingyuan
- 机构：Beijing Forestry Univ;East China Univ Sci & Technol
- 来源：ACS BIOMATERIALS SCIENCE & ENGINEERING 2018
- 关键词：polymeric nanoparticle drug delivery lignin renewable biopolymer self-assembly hydroxyl camptothecin
- Biodegradation of benzene homologues in contaminated sediment of the East China Sea被引量：43收藏
- 作者：Li, Hui Zhang, Qian Wang, Xiao-Li Ma, Xing-Yuan Lin, Kuang-Fei Liu, Yong-Di Gu, Ji-Dong Lu, Shu-Guang Shi, Lei Lu, Qiang Shen, Ting-Ting
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;Univ Hong Kong
- 来源：BIORESOURCE TECHNOLOGY 2012
- 关键词：BTEX Biodegradation Bacterial community Metabolic pathway Compatible solutes
- Development of a fed-batch process for the production of anticancer drug TATm-survivin(T34A) in Escherichia coli被引量：40收藏
- 作者：Zhang, Haiyi Zheng, Yu Liu, Qinghai Tao, Xinyi Zheng, Wenyun Ma, Xingyuan Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biochemical Engineering Journal 2009
- 关键词：Batch cell culture - Controlled drug delivery - Recombinant protEIns - Batch data processing - Gene expression
- High-level secretion and purification of recombinant acetylcholinesterase from human cerebral tissue in P. pastoris and identification by chromogenic reaction被引量：30收藏
- 作者：Ma, Xingyuan Tan, Jianhua Wei, Dongzhi Zhu, Pin Sun, Manji
- 机构： State Key Laboratory of Bioreactor Engineering; Gene Engineering Key Laboratory of PLA; Institute of Pharmacology and Toxicology
- 来源：Applied Microbiology and Biotechnology 2006
- 关键词：Affinity chromatography - Chromogenics - Cloning - Ion exchange - Methanol - Tissue
- Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene被引量：30收藏
- 作者：Wang, Tianwen Zhu, Hu Ma, Xingyuan Fei, Zhuoya Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2006
- 关键词：Antibiotics - Biotechnology - Cells - Escherichia coli - Genes
- Over-expression of glycerol dehydrogenase and1,3-propanediol oxidoreductase in Klebsiella pneumoniae and thEIr effects on conversion of glycerol into 1,3-propanediol in resting cell system被引量：28收藏
- 作者：Zhao, Li Ma, Xingyuan Zheng, Yu Zhang, Jianguo Wei, Guodong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Chemical Technology and Biotechnology 2009
- 关键词：Cytology - Bacteria - Cells
- Effects of over-expression of glycerol dehydrogenase and 1,3-propanediol oxidoreductase on bioconversion of glycerol into 1,3-propandediol by Klebsiella pneumoniae under micro-aerobic conditions被引量：28收藏
- 作者：Zhao, Li Zheng, Yu Ma, Xingyuan Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioprocess and Biosystems Engineering 2009
- 关键词：Bacteria - Lactic acid - Ethanol - Enzymes
- High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro被引量：23收藏
- 作者：Ma, Xingyuan Zheng, Wenyun Wei, Dongzhi Ma, Yushu Wang, Tianwen Wang, Jinzhi Liu, Qinghai Yang, Shengli
- 机构：E China Univ Sci & Technol;Shenyang Pharmaceut Univ;Chinese Acad Sci
- 来源：JOURNAL OF BIOTECHNOLOGY 2006
- 关键词：TAT-survivin (T34A) mutant high-level expression and purification pro-apoptosis
- A novel liposome-polymer hybrid nanoparticles delivering a multi-epitope self-replication DNA vaccine and its preliminary immune evaluation in experimental animals被引量：23收藏
- 作者：Zhao, Zhangting Ma, Xingyuan Zhang, Ruihuan Hu, Fabiao Zhang, Tong Liu, Yuping Han, Myong Hun You, Fang Yang, Yi Zheng, Wenyun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;KIM IL SUNG Univ;Natl Univ Singapore;SinGENE Biotech Pte Ltd
- 来源：NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 2021
- 关键词：DNA vaccine Liposome-polymer hybrid nanoparticles Delivery Immune evaluation Infectious diseases
- Overview of Regulatory Strategies and Molecular Elements in Metabolic Engineering of Bacteria被引量：19收藏
- 作者：Wang, Tianwen Ma, Xingyuan Du, Guocheng Chen, Jian
- 机构：E China Univ Sci & Technol;Jiangnan Univ
- 来源：MOLECULAR BIOTECHNOLOGY 2012
- 关键词：Metabolic pathway Metabolic engineering Riboswitch Intergenic region Rational design Directed evolution
- Production of glycerol from glucose by coexpressing glycerol-3-phosphate dehydrogenase and glycerol-3-phosphatase in Klebsiella pneumoniae被引量：19收藏
- 作者：Zheng, Yu Zhao, Li Zhang, Jianguo Zhang, Haiyi Ma, Xingyuan Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Bioscience and Bioengineering 2008
- 关键词：Recombinant protEIns - Bacteria - Glucose - Phosphatases
- One-step production of 2,3-butanediol from starch by secretory over-expression of amylase in Klebsiella pneumoniae被引量：19收藏
- 作者：Zheng, Yu Zhang, Haiyi Zhao, Li Wei, Liujing Ma, Xingyuan Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Chemical Technology and Biotechnology 2008
- 关键词：Bacteria - Amylases - Gene expression - Substrates
- Available methods for assembling expression cassettes for synthetic biology被引量：19收藏
- 作者：Wang, Tianwen Ma, Xingyuan Zhu, Hu Li, Aitao Du, Guocheng Chen, Jian
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;Jiangnan Univ;China Univ Petr E China
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2012
- 关键词：Synthetic biology Simultaneous expression Pathway construction Yeast recombination Cre-loxP site-specific recombination Acembl system
- Discovery of the cell-penetrating function of A2 domain derived from LTA subunit of Escherichia coli heat-labile enterotoxin被引量：15收藏
- 作者：Liu, Di Guo, Hua Zheng, Wenyun Zhang, Na Wang, Tianwen Wang, Ping Ma, Xingyuan
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Heat-labile enterotoxin (LT) A2 domain Cell-penetrating Multi-endocytosis pathway Drugs delivery
- Selective extraction of bioactive glycoprotEIn in neutral environment through Concanavalin A mediated template immobilization and dopamine surface imprinting被引量：13收藏
- 作者：Qu, Xue Wang, Feifei Sun, Yi Tian, Yu Chen, Rui Ma, Xiaoyu Liu, Changsheng
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：RSC ADVANCES 2016
- 关键词：Extraction - ProtEIns - Dichroism - Magnetite - Amines - Spectrum analysis - Binding energy - Diagnosis - Adsorption - Neurophysiology
- Multiple targeting strategies achieve novel protEIn drug delivery into proapoptosis lung cancer cells by precisely inhibiting survivin被引量：8收藏
- 作者：Hu, Fabiao Yan, Ting Guo, Wei Liu, Qiuli Han, Myong Hun Liu, Chang Liu, Yuping Zheng, Wenyun You, Fang Yang, Yi Zhang, Wenliang Ma, Xingyuan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;KIM IL SUNG Univ;Natl Univ Singapore;SinGENE Biotech Pte Ltd
- 来源：NANOSCALE 2020
- 关键词：Cancer cells - Tumors - Cell death - Mammals - Diseases - Targeted drug delivery - Biocompatibility - Controlled drug delivery - Recombinant protEIns
- Cloning, expression, purification and bioactivity evaluation of a thrombin-like enzyme from DEInagkistrodon acutus venom gland library被引量：7收藏
- 作者：Li, An Zhang, Chuan Wang, Jie Wang, Junjie Jiang, Hailong Li, Jianzhong Ma, Xingyuan Zhang, Wen Lu, Yiming
- 机构：Second Mil Med Univ;East China Univ Sci & Technol; Second Mil Med Univ
- 来源：BIOTECHNOLOGY LETTERS 2018
- 关键词：DEInagkistrodon acutus Blood clotting Fibrinogenolytic activity Phage display Snake venom Thrombin-like enzyme
- 3,4,5-O-tricaffeoylquinic acid alleviates ionizing radiation-induced injury in vitro and in vivo through regulating ROS/JNK/p38 signaling被引量：7收藏
- 作者：Liu, Jiajun Hu, Wen Ma, Xiaoying Liang, Xin Lin, Long Huang, Jianming Liu, Jianwen
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Fudan Univ
- 来源：ENVIRONMENTAL TOXICOLOGY 2022
- 关键词：3 4 5-O-tricaffeoylquinic acid apoptosis ionizing radiation MAPKs ROS
- Spatially programmed assembling of oxidoreductases with single-stranded DNA for cofactor-required reactions被引量：6收藏
- 作者：Wang, Tianwen David Ma, Fei Ma, Xingyuan Wang, Ping
- 机构：E China Univ Sci & Technol;Univ Minnesota
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Coupled oxidoreductive reaction Cofactor recycling Spatially assembled enzymes Single-stranded DNA Substrate channeling Proximity effect
- Development of a synthetic library of humanized nanobodies for targeted IL-6 inhibition被引量：6收藏
- 作者：Wang, Lei Dong, Jiayi Wu, Chenlu Yan, Chenyue Bi, Chong Xu, Chengnan Wu, Yiling Zheng, Wenyun Ma, Xingyuan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 2024
- 关键词：nanobody humanization stability synthetic library phage display interleukin-6 blocking antibody
- A nanobody-based molecular toolkit for ubiquitin-proteasome system explores the main role of survivin subcellular localization被引量：6收藏
- 作者：Miao, Hui Liu, Chang Ouyang, Hao Zhang, Peiwen Liu, Yuping Zhang, Chen Deng, Changping Fu, Yunhui Niu, Jinping Zheng, Wenyun You, Fang Yang, Yi Ma, Xingyuan
- 机构：East China Univ Sci & Technol;Shanghai Univ Tradit Chinese Med;East China Univ Sci & Technol;Natl Univ Singapore
- 来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 2023
- 关键词：survivin subcellular function nanobody targeted TRIM21 apoptosis and cell cycle anti-cancer
- Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protEIn nanocages for enhancing cancer biotherapy被引量：5收藏
- 作者：Hu, Fabiao Deng, Changping Zhou, Yiwen Liu, Yuping Zhang, Tong Zhang, Peiwen Zhao, Zhangting Miao, Hui Zheng, Wenyun Zhang, Wenliang Wang, Meiyan Ma, Xingyuan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Univ North Carolina Greensboro;East China Normal Univ;East China Normal Univ
- 来源：BIOENGINEERING & TRANSLATIONAL MEDICINE 2022
- 关键词：cancer precision therapy ferritin heavy chain nanocages matrix metalloprotEInase-2 survivin transcription and protEIn levels
- Construction and application of a multifunctional CHO cell platform utilizing Cre/lox and Dre/rox site-specific recombination systems被引量：5收藏
- 作者：Zhang, Chen Chang, Feng Miao, Hui Fu, Yunhui Tong, Xikui Feng, Yu Zheng, Wenyun Ma, Xingyuan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 2023
- 关键词：CHO Cre/lox Dre/rox CRISPR/Cas9 site-specific recombination hotspot production stability
- pH-sensitive pHluorins as a molecular sensor for in situ monitoring of enzyme-catalyzed prodrug activation被引量：3收藏
- 作者：Liu, Hui Cao, Xiaodan Wang, Ping Ma, Xingyuan
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY 2017
- 关键词：Bcap-37 enzyme prodrug therapy HRP IAA ratiometric pHluorins
- Design and evaluation of ionically crosslinked multifunctional ELP-SA composite hydrogels for 3D cell culture被引量：2收藏
- 作者：Chen, Yiying Wang, Yangmin Li, Yuxi Chen, Xingyang Zheng, Wenyun Wang, Tianwen Jia, Hao Ma, Xingyuan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;Anhui Polytech Univ;Shanghai Jiao Tong Univ
- 来源：REGENERATIVE BIOMATERIALS 2025
- 关键词：elastin-like polypeptide sodium alginate composite hydrogel 3D cell culture
- Screening and construction of nanobodies against human CD93 using phage libraries and study of thEIr antiangiogenic effects被引量：2收藏
- 作者：Miao, Hui Wu, Yiling Ouyang, Hao Zhang, Peiwen Zheng, Wenyun Ma, Xingyuan
- 机构：East China Univ Sci & Technol;Shanghai Univ Tradit Chinese Med;East China Univ Sci & Technol
- 来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY 2024
- 关键词：CD93 nanobody phage display angiogenesis vascular permeability
- A Novel Method for Efficient Preparation of Mucosal Adjuvant Escherichia coli Heat-Labile Enterotoxin Mutant (LTm) by Artificially Assisted Self-Assembly In Vitro被引量：1收藏
- 作者：Liu, Di Zhang, Na Zheng, Wenyun Guo, Hua Wang, Xiaoli Wang, Tianwen Wang, Ping Ma, Xingyuan
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2016
- 关键词：Heat-labile enterotoxin (LT) Mucosal adjuvant In vitro preparation Artificially assisted self-assembly
- TGF-β1 fused to elastin-like polypeptides nanoparticles with kartogenin loading for mesenchymal stem cell directed cartilage differentiation and promoting in vivo repair被引量：1收藏
- 作者：Fu, Yunhui Li, Yuxi Wu, Chenlu Chen, Yiying Zhang, Chen Tong, Xikui Wang, Yangmin Shen, Yue Zheng, Wenyun Ma, Xingyuan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF MATERIALS CHEMISTRY B 2026
- 关键词：Cartilage - Cell culture - Elastin - Hyaluronic acid - Hydrogels - Nanoparticles - Pathology - Repair - Transmission electron microscopy
