成果/Result
- High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells被引量：36收藏
- 作者：Wu, Yixiao Bissinger, Thomas Genzel, Yvonne Liu, Xuping Reichl, Udo Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Max Planck Inst Dynam Complex Tech Syst;Shanghai BioEngine Sci Tech Co Ltd;Otto von Guericke Univ
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2021
- 关键词：MDCK cells Influenza A virus ATF-based perfusion Process optimization Lab-scale bioreactors
- Identification of multiple sources of the acidic charge variants in an IgG1 monoclonal antibody被引量：35收藏
- 作者：Miao, Shiwei Xie, Panpan Zou, Mao Fan, Li Liu, Xuping Zhou, Yan Zhao, Liang Ding, Ding Wang, Haibin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2017
- 关键词：mAb IgG1 Acidic charge heterogenEIty Deamidation Galactosylation Disulfide bond reduction
- Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media: cell growth, antibody production, metabolic waste, glutamate, and energy metabolism被引量：34收藏
- 作者：Zhang, Li-xiang Zhang, Wei-yan Wang, Chen Liu, Jin-tao Deng, Xian-cun Liu, Xu-ping Fan, Li Tan, Wen-song
- 机构： The State Key Laboratory of Bioreactor Engineering; Zhejiang Hisun Pharmaceutical Co. Ltd
- 来源：Bioresources and Bioprocessing 2016
- 关键词：Amino acids - Cell culture - Cell growth - Growth kinetics - Monoclonal antibodies - Physiology
- Elucidating the effects of pH shift on IgG1 monoclonal antibody acidic charge variant levels in Chinese hamster ovary cell cultures被引量：33收藏
- 作者：Xie, Panpan Niu, Huijie Chen, Xinning Zhang, Xintao Miao, Shiwei Deng, Xiancun Liu, Xuping Tan, Wen-Song Zhou, Yan Fan, Li
- 机构：East China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Acidic charge variants IgG1 monoclonal antibody Disulfide bond reduction Galactosylation Deamination Liquid chromatography mass spectrometry
- Galactose supplementation enhance sialylation of recombinant Fc-fusion protEIn in CHO cell: an insight into the role of galactosylation in sialylation被引量：31收藏
- 作者：Liu, Jintao Wang, Jie Fan, Li Chen, Xinning Hu, Dongdong Deng, Xiancun Poon, H. Fai Wang, Haibin Liu, Xuping Tan, Wen-Song
- 机构：E China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Hangzhou Co Ltd
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2015
- 关键词：Fc-fusion protEIn CHO cell Glycosylation dSialylation Galactose Galactosylation
- Detailed understanding of enhanced specific productivity in Chinese hamster ovary cells at low culture temperature被引量：26收藏
- 作者：Kou, Tian-Ci Fan, Li Zhou, Yan Ye, Zhao-Yang Liu, Xu-Ping Zhao, Liang Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2011
- 关键词：Chinese hamster ovary (CHO) cells TNFR-Fc mRNA stability Transcriptional rate Low temperature
- Culture temperature modulates monoclonal antibody charge variation distribution in Chinese hamster ovary cell cultures被引量：20收藏
- 作者：Zhang, Xintao Sun, Ya-Ting Tang, Hongping Fan, Li Hu, Dongdong Liu, Jintao Liu, Xuping Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2015
- 关键词：Basic carboxypeptidase Charge variation Chinese hamster ovary cells Culture temperature Lysine variation Monoclonal ntibodies Weak cation exchange chromatography
- Investigation into the impact of tyrosine on the product formation and quality attributes of mAbs in rCHO cell cultures被引量：16收藏
- 作者：Zhang, Weijian Liu, Xuping Tang, Hongping Zhang, Xinran Zhou, Yanan Fan, Li Wang, Haibin Tan, Wen-Song Zhao, Liang
- 机构：East China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2020
- 关键词：Chinese hamster ovary cells Tyrosine Fed-batch cultures Critical quality attribute CD20 Monoclonal antibody
- Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose被引量：15收藏
- 作者：Sun, Ya-ting Zhao, Liang Ye, Zhaoyang Fan, Li Liu, Xu-ping Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2013
- 关键词：CHO cells Fed-batch culture Antibody production Lactate metabolism Galactose Process development
- Chinese hamster ovary cell performance enhanced by a rational divide-and-conquer strategy for chemically defined medium development被引量：12收藏
- 作者：Liu, Yaya Zhang, Weiyan Deng, Xiancun Poon, Hong Fai Liu, Xuping Tan, Wen-Song Zhou, Yan Fan, Li
- 机构：E China Univ Sci & Technol;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2015
- 关键词：Basal medium design Chemical-defined Chinese hamster ovary High-yielding Monoclonal antibody
- MOI对HEK293细胞感染病毒后的生长代谢和腺病毒扩增效率的影响被引量：12收藏
- 作者：刘旭平 范里 朱明龙 张旭 谭文松
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《高校化学工程学报》 2010
- 关键词：HEK293细胞 腺病毒 病毒扩增效率 MOI
- 摘要：分别在方瓶贴壁和转瓶悬浮二种培养模式下,考察了感染复数(Multiplicity of Infection,MOI)对HEK293细胞感染病毒后的生长代谢和腺病毒扩增效率的影响。通过实验发现,感染病毒后的细胞跟未感染病毒...
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- Understanding the intracellular effects of yeast extract on the enhancement of Fc-fusion protEIn production in Chinese hamster ovary cell culture被引量：11收藏
- 作者：Hu, Dongdong Sun, Yating Liu, Xuping Liu, Jintao Zhang, Xintao Zhao, Liang Wang, Haibin Tan, Wen-Song Fan, Li
- 机构：E China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Chinese hamster ovary cells Yeast extract Fc-fusion protEIn Specific productivity Cell cycle arrest mTOR
- A novel method based on nonparametric regression with a Gaussian kernel algorithm identifies the critical components in CHO media and feed optimization被引量：11收藏
- 作者：Zou, Mao Zhou, Zi-Wei Fan, Li Zhang, Wei-Jian Zhao, Liang Liu, Xu-Ping Wang, Hai-Bin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Shanghai Bioengine Scitech Co Ltd;Hisun Pharmaceut Hangzhou Co Ltd
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2020
- 关键词：Variable selection Nonparametric regression with Gaussian kernel Chinese hamster ovary cells Medium optimization
- Elucidating the effects of arginine and lysine on a monoclonal antibody C-terminal lysine variation in CHO cell cultures被引量：10收藏
- 作者：Zhang, Xintao Tang, Hongping Sun, Ya-Ting Liu, Xuping Tan, Wen-Song Fan, Li
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Chinese hamster ovary cells Monoclonal antibody Lysine variant Arginine Lysine Carboxypeptidase
- Insights into the generation of monoclonal antibody acidic charge variants during Chinese hamster ovary cell cultures被引量：8收藏
- 作者：Tang, Hongping Miao, Shiwei Zhang, Xintao Fan, Li Liu, Xuping Tan, Wen-Song Zhao, Liang
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2018
- 关键词：Chinese hamster ovary cells Monoclonal antibody Charge heterogenEIty Acidic variants Asparagine deamidation
- Theoretical study of zeatin - A plant hormone and potential drug for neural diseases - On the basis of DFT, MP2 and target docking被引量：8收藏
- 作者：Liu, Xueping Berezniak, Tomasz Panek, Jaroslaw Jan Jezierska-Mazzarello, Aneta
- 机构：E China Univ Sci & Technol;Univ Wroclaw
- 来源：CHEMICAL PHYSICS LETTERS 2013
- Highly efficient production of an influenza H9N2 vaccine using MDCK suspension cells被引量：7收藏
- 作者：Wu, Yixiao Jia, Hanjing Lai, Hanzhang Liu, Xuping Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Guangdong Wens Dahuanong Biotechnol Co Ltd;Zhaoqing Dahuanong Biol Med Co Ltd;Shanghai BioEngine Sci Tech Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2020
- 关键词：MDCK suspension cells Cell adaptation Influenza virus Feed strategy Cell culture-based vaccine manufacturing
- Uridine modulates monoclonal antibody charge heterogenEIty in Chinese hamster ovary cell fed-batch cultures被引量：7收藏
- 作者：Niu, Huijie Wang, Jiaqi Liu, Mengjuan Chai, Miaomiao Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Charge heterogenEIty Chinese hamster ovary cells Medium components Monoclonal antibody Uridine
- Biphasic addition strategy of hypoxanthine and thymidine for improving monoclonal antibody production被引量：6收藏
- 作者：Chen, Fei Ye, Zhaoyang Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2012
- 关键词：Chinese hamster ovary cells Hypoxanthine Thymidine Monoclonal antibody production Fed-batch culture
- Characterization and minimization of sialic acid degradation in an Fc-fusion protEIn-producing CHO cell bioprocess被引量：6收藏
- 作者：Chen, Xinning Liu, Jintao Liu, Xuping Fan, Li Zhao, Liang Tan, Wen-Song
- 机构：East China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2018
- 关键词：Chinese hamster ovary cells Fc-fusion protEIn Sialic acid Sialidase pH
- Physiological responses of Chinese hamster ovary cells to a productivity-enhancing yeast extract被引量：5收藏
- 作者：Hu, Dongdong Zhao, Liang Wang, Jiaqi Fan, Li Liu, Xuping Wang, Haibin Tan, Wen-Song
- 机构：East China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Co Ltd
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2018
- 关键词：Chinese hamster ovary cells Yeast extract Fc-fusion protEIn Specific productivity Amino acid metabolism Energy charge
- Correlation of antibody production rate with glucose and lactate metabolism in Chinese hamster ovary cells被引量：5收藏
- 作者：Chen, Fei Ye, Zhaoyang Zhao, Liang Liu, Xuping Fan, Li Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2012
- 关键词：Antibody production Cell metabolism Chinese hamster ovary cells Mitochondrial dehydrogenase activity
- Insights into the loss of protEIn sialylation in an fc-fusion protEIn-producing CHO cell bioprocess被引量：5收藏
- 作者：Chen, Xinning Liu, Xuping Xiao, Zheng Liu, Jintao Zhao, Liang Tan, Wen-Song Fan, Li
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2019
- 关键词：Chinese hamster ovary cells Fc-fusion protEIn Sialylation Extracellular degradation Intracellular biosynthesis
- Process analysis of reduced specific productivity of TNFR-Fc in Chinese hamster ovary cells at high cell density被引量：5收藏
- 作者：Kou, Tian-Ci Fan, Li Ye, Zhao-Yang Zhou, Yan Liu, Xu-Ping Zhao, Liang Tan, Wen-Song
- 机构：E China Univ Chem Technol
- 来源：PROCESS BIOCHEMISTRY 2011
- 关键词：Cell density CHO cells TNFR-Fc Specific productivity Process analysis
- Application of improved top-down approach in maximizing CHO cell mass and productivity in fed-batch culture被引量：5收藏
- 作者：Sun, Ya-Ting Zhao, Liang Liu, Xu-Ping Hu, Dong-dong Fan, Li Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 2013
- 关键词：CHO cells top-down approach fed-batch culture monoclonal antibody amino acids
- Effects of Calcium Ion on Adenovirus Production with High Densities of HEK293 Cells被引量：3收藏
- 作者：Liu, Xuping Zhao, Liang Wang, Yigang Zhang, Xu Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 2010
- 关键词：adenovirus calcium ion cell density HEK293 cells virus production
- The improvement of adenovirus vector production by increased expression of coxsackie adenovirus receptor被引量：2收藏
- 作者：Liu, Xuping Wang, Yigang Niu, Hongxing Zhang, Xu Tan, Wen-Song
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2009
- 关键词：Adenovirus CAR Cell density effect Cell-specific productivity HEK 293 cells
- Monitoring sialylation levels of Fc-fusion protEIn using size-exclusion chromatography as a process analytical technology tool被引量：1收藏
- 作者：Liu, Jintao Chen, Xinning Fan, Li Deng, Xiancun Poon, H. Fai Tan, Wen-Song Liu, Xuping
- 机构：E China Univ Sci & Technol;Zhejiang Hisun Pharmaceut Hangzhou Co Ltd
- 来源：BIOTECHNOLOGY LETTERS 2015
- 关键词：Cell culture Critical quality attributes Critical quality parameters Fc-fusion protEIn Glycosylation Process analytical technology SEC-HPLC Sialic acid
- 基于Box-Behnken实验设计与响应面法优化禽流感疫苗生产工艺?被引量：0收藏
- 作者：胡可可 刘旭平 蔡海波 谭文松
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《高校化学工程学报》 2019
- 关键词：MDCK细胞悬浮培养 禽流感病毒疫苗 部分析因实验设计 响应面方法 BBD实验设计
- 摘要：为优化基于MDCK细胞无血清悬浮培养生产禽流感病毒的工艺以提高禽流感病毒血凝素(HA)滴度,采用部分析因实验设计考察了病毒生产工艺中接毒时细胞密度、TPCK-胰酶浓度、培养温度、感染复数和病毒收获时间对病毒HA滴度的影响...
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- Correction to: High cell density perfusion process for high yield of influenza A virus production using MDCK suspension cells (Applied Microbiology and Biotechnology, (2021), 105, 4, (1421-1434), 10.1007/s00253-020-11050-8)被引量：-1收藏
- 作者：Liu, Xuping
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2021
