成果/Result
- High-Throughput System for Screening of cephalosporin C High-Yield Strain by 48-Deep-Well Microtiter Plates被引量：30收藏
- 作者：Tan, Jun Chu, Ju Hao, Yuyou Guo, Yuanxin Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2013
- 关键词：cephalosporin C High-throughput screening High-throughput bioactivity assay 48-deep MTP Mixture culture
- High-throughput screening strategy used for enhanced production of pigment by Monascus purpureus D39-4被引量：29收藏
- 作者：Tan, Jun Chu, Ju Shi, Wenjuan Lin, Cheng Guo, Yuanxin Zhuang, Yingping Zhang, Siliang Imanaka, Tadayuki
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Food Science and Biotechnology 2012
- 关键词：Nitrogen compounds - Bottles - Sodium nitrate - Screening - Throughput
- RQ feedback control for simultaneous improvement of GSH yield and GSH content in Saccharomyces cerevisiae T65被引量：27收藏
- 作者：Xiong, Zhi-Qiang Guo, Mei-Jin Guo, Yuan-Xin Chu, Ju Zhuang, Ying-Ping Wang, Nam Sun Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Chemical and Biomolecular Engineering
- 来源：Enzyme and Microbial Technology 2010
- 关键词：Oxygen - Proportional control systems - Two term control systems - Glucose - Peptides - Yeast - Ethanol - carbon dioxide - Productivity
- Real-time viable-cell mass monitoring in high-cell-density fed-batch glutathione fermentation by Saccharomyces cerevisiae T65 in industrial complex medium被引量：23收藏
- 作者：Xiong, Zhi-Qiang Guo, Mei-Jin Guo, Yuan-Xin Chu, Ju Zhuang, Ying-Ping Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Bioscience and Bioengineering 2008
- 关键词：Batch cell culture - carbon dioxide - Fermentation - Yeast
- Efficient extraction of intracellular reduced glutathione from fermentation broth of Saccharomyces cerevisiae by ethanol被引量：16收藏
- 作者：Xiong, Zhi-Qiang Guo, Mei-Jin Guo, Yuan-Xin Chu, Ju Zhuang, Ying-Ping Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2009
- 关键词：Extraction - Ethanol - Fermentation - Energy utilization - Peptides
- Artificial intelligence-guided fermentation process regulation reveals metabolic rewiring in gentamicin C1a overproduction被引量：10收藏
- 作者：Xu, Feng Su, Lihuan Wang, Yuan Gao, Hao Hu, Kaihao Ben, Rong Guo, Yuanxin Li, Xu Chu, Ju Tian, Xiwei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2025
- 关键词：Fermentation optimization Artificial neural network Multi-sensor monitoring Genetic algorithm Metabolic flux analysis
- Engineering the methyltransferase through inactivation of the genK and genL leads to a significant increase of gentamicin C1a production in an industrial strain of Micromonospora echinospora 49-92S被引量：10收藏
- 作者：Xu, Feng Zhang, Xinyu Liu, Ling Ke, Xiang Wu, Jie Guo, Yuanxin Tian, Xiwei Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2022
- 关键词：Micromonospora echinospora Gentamicin C1a Methyltransferase GenK-genL Metabolic flux analysis
- 葡萄糖酸钙对肌苷发酵的影响被引量：10收藏
- 作者：陈双喜 郭元昕 储炬 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2005
- 关键词：枯草杆菌 肌苷 发酵 葡萄糖酸钙 葡萄糖酸激酶
- 摘要：在肌苷的摇瓶发酵过程中,10 g/L的葡萄糖酸钙适于肌苷的合成和菌体生长。初始培养基中加入10 g/L的葡萄糖酸钙,能够诱导葡萄糖酸激酶的生成,大幅提高其比活,增大磷酸戊糖(HM P)途径的通量。肌苷的产率由10.76 ...
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- 基于参数相关分析的头孢菌素C发酵过程溶氧调控策略被引量：8收藏
- 作者：李景森 庄英萍 王永红 郭元昕 储炬
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2007
- 关键词：头孢菌素C 参数相关分析 溶氧 菌形 豆油
- 摘要：运用多参数检测的生物反应器及其相关计算机软、硬件技术,对头孢菌素C发酵过程中表征细胞代谢的宏观代谢特征参数进行了检测与相关性分析,表明发酵前期菌体形态分化有利于增加溶氧,膨大、断裂菌丝的形成能够启动头孢菌素C合成;发酵中...
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- 枯草芽胞杆菌肌苷发酵过程参数相关分析被引量：6收藏
- 作者：陈双喜 周潜 郭元昕 储炬 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：相关分析 枯草芽胞杆菌 肌苷 发酵 过程优化
- 摘要：基于参数相关的研究方法已成功地应用于多个发酵产品的过程优化,本文通过对枯草芽胞杆菌肌苷生产过程的尾气变化规律、碳氮源消耗和产物浓度的分析和计算,并根据摄氧率(OUR)、二氧化碳释放率(ceR)、呼吸熵(RQ)和产物得率(...
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- Optimization and validation of an extraction method and HPAEC-PAD for determination of residual sugar composition in L-lactic acid industrial fermentation broth with a high salt content被引量：5收藏
- 作者：Lv, Xiangyun Guo, Yuanxin Zhuang, Yingping Wang, Yonghong
- 机构：E China Univ Sci & Technol;Shanghai Inst Biomfg Technol;Collaborat Innovat Ctr
- 来源：ANALYTICAL METHODS 2015
- 关键词：calcium - Ion exchange - Extraction - Ion chromatography - Fermentation - Starch - Ions - Sodium hydroxide
- 不同有机氮源对肌苷合成的影响被引量：4收藏
- 作者：郭元昕 陈双喜 刘新星 储炬 庄英萍
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：肌苷 发酵 有机氮源 蛋白 核酸
- 摘要：通过研究不同有机氮源对肌苷合成的影响,结合蛋白、核酸及菌体生长的分析测定,结果显示:国产酵母膏A是肌苷发酵的理想氮源。此外,由于国产酵母膏A部分可溶,其水解出的氨基酸的碳骨架可能作为前体物质直接进入糖酵解和三羧酸途径,使...
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- Oxygen uptake rate (OUR) guided nitrogen control strategy for improving nemadectin production by Streptomyces cyaneogriseus ssp. noncyanogenus被引量：2收藏
- 作者：Song, Xiaoqing Zhang, Zishu Yu, Junxiong Zhang, Yun Xue, Jiayun Guo, Yuanxin Liang, Jianguang Ren, Min Ling, Qingyun Mohsin, Ali Qian, Jiangchao Wang, Zejian Wang, Yonghong
- 机构：East China Univ Sci & Technol;Changshu Inst Technol;Jiangsu Weiling Pharmaceut Co Ltd
- 来源：PROCESS BIOCHEMISTRY 2025
- 关键词：Nemadectin S. cyaneogriseus On-line parameters Real-time monitoring Fed-batch fermentation
- Systematic engineering of Micromonospora echinospora cell factory for gentamicin C1a overproduction被引量：1收藏
- 作者：Xu, Feng Gao, Hao Ben, Rong Hu, Kaihao Wang, Yuan Mohsin, Ali Guo, Yuanxin Li, Xu Hang, Haifeng Chu, Ju Tian, Xiwei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：TRENDS IN BIOTECHNOLOGY 2026
- 关键词：Antibiotics - Biochemistry - Biosynthesis - Genes - Metabolism - Optimization
- 人α2a干扰素酵母工程菌高产培养条件的研究被引量：1收藏
- 作者：储炬 蔡海波 胡千德 郭元昕 叶勤 徐伦
- 机构：华东理工大学生物反应器工程国家重点实验室;上海万兴生物制品有限公司
- 来源：《华东理工大学学报（自然科学版）》 2000
- 关键词：α2a型干扰素 发酵 培养条件 酵母工程菌
- 摘要：研究了摇瓶培养条件对人α2 a干扰素酵母工程菌生长及表达的影响 ,结果表明 ,当基础料中腺嘌呤含量为 2 0μg/m L,在发酵进行至 1 2 h时再加入 2 0μg/m L腺嘌呤时 ,IFN-α2 a生物活性及比活分别...
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- Correction: Artificial intelligence-driven dynamic regulation for high-efficiency gentamicin C1a production被引量：-1收藏
- 作者：Xu, Feng Wang, Yuan Gao, Hao Hu, Kaihao Ben, Rong Mohsin, Ali Guo, Yuanxin Li, Xu Wu, Hui Hang, Haifeng Chu, Ju Tian, Xiwei
- 机构： State Key Laboratory of Bioreactor Engineering; National Center of Bio-Engineering & Technology ; Shanghai Collaborative Innovation Center for Biomanufacturing Technology
- 来源：Green Chemistry 2026
