成果/Result
- Nitrogen metabolism of asparagine and glutamate in Vero cells studied by 1H/15N NMR spectroscopy被引量：52收藏
- 作者：Huang, Haiyan Yu, Yihua Yi, Xiaoping Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai Key Laboratory of Functional Magnetic Resonance Imaging
- 来源：Applied Microbiology and Biotechnology 2007
- 关键词：Ammonia - Metabolism - Metabolites - Nitrogen - Nuclear magnetic resonance spectroscopy
- Regulation of porcine circovirus type 2-like particles expressed in baculovirus expression system被引量：35收藏
- 作者：Li, Yingjun Yi, Xiaoping Zhuang, Yingping Chu, Ju
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2016
- 关键词：Animals - Transcription - Viruses
- Metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine被引量：32收藏
- 作者：Zhang, Fang Sun, Xiangming Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：CYTOTECHNOLOGY 2006
- 关键词：animal cell culture cell metabolism glutamine glutamine synthetase recombinant CHO cell
- Novel scale-up strategy based on three-dimensional shear space for animal cell culture被引量：22收藏
- 作者：Li, Chao Teng, Xiaonuo Peng, Huadong Yi, Xiaoping Zhuang, Yingping Zhang, Siliang Xia, Jianye
- 机构：East China Univ Sci & Technol;Imperial Coll London;Imperial Coll London
- 来源：CHEMICAL ENGINEERING SCIENCE 2020
- 关键词：Shear rate Animal cell culture Scale-up Computational fluid dynamics Stirred tank bioreactor
- Application of semi-supervised convolutional neural network regression model based on data augmentation and process spectral labeling in Raman predictive modeling of cell culture processes被引量：22收藏
- 作者：Min, Rui Wang, Zhi Zhuang, Yingping Yi, Xiaoping
- 机构：East China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2023
- 关键词：Semi -supervised learning Convolutional neural network regression Data augmentation Process spectra labeling
- Gene transcription acceleration: Main cause of hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells被引量：19收藏
- 作者：Wang, Wenying Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2007
- 关键词：cell culture CHO cell dimethyl sulfoxide (DMSO) hepatitis B surface antigen gene transcription
- Improvement of Vero cell growth in glutamate-based culture by supplementing ammoniagenic compounds被引量：13收藏
- 作者：Huang, Haiyan Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2006
- 关键词：alanine asparagine glutamate glutamine-free glutamine synthetase Vero cell
- Hydrodynamic investigation of a novel shear-generating device for the measurement of anchorage-dependent cell adhesion intensity被引量：11收藏
- 作者：Wang, Han Xia, Jianye Zheng, Zhiming Zhuang, Ying-Ping Yi, Xiaoping Zhang, Dahe Wang, Peng
- 机构：Chinese Acad Sci;Univ Sci & Technol China;East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2018
- 关键词：Animal cell culture Adhesion force Energy dissipation rate Microcarriers Modeling
- Tumor microenvironment responsive multifunctional smart living materials based on engineered bacteria for inducing macrophage polarization to enhance tumor immunotherapy被引量：11收藏
- 作者：Jiang, Ning Zhao, Kui Liu, Chengcheng Zhu, Xiaojuan Huang, Xumeng Yang, Lin Yi, Xiaoping Zhuang, Yingping Ye, Bangce Qian, Jiangchao Huang, Jiaofang
- 机构：East China Univ Sci & Technol ECUST;East China Univ Sci & Technol;Xian Jiao Tong Univ Hlth Sci Ctr;Jiangxi Normal Univ JXNU;East China Univ Sci & Technol
- 来源：CHEMICAL ENGINEERING JOURNAL 2024
- 关键词：Biomaterials Biotechnology Engineered living material Targeted therapy Drug delivery
- Large-scale plasmid preparation for transient gene expression被引量：10收藏
- 作者：Cheng, Lu Sun, Xiangming Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Kexin Biotech Co Ltd
- 来源：BIOTECHNOLOGY LETTERS 2011
- 关键词：CHO cells Fed-batch Plasmid DNA RNase Transient transfection Ultrafiltration
- Coupling metabolomics analysis and DOE optimization strategy towards enhanced IBDV production by chicken embryo fibroblast DF-1 cells被引量：10收藏
- 作者：Lin, Jia Yi, Xiaoping Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2020
- 关键词：DF-1 IBDV Metabolome DOE methodology
- Enhanced intracellular accumulation of recombinant HBsAg in CHO cells by dimethyl sulfoxide被引量：10收藏
- 作者：Ma, Zheyong Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2008
- 关键词：cell culture recombinant CHO cell dimethyl sulfoxide HBsAg sucrose gradient analysis intracellular accumulation
- Surface modification of non-woven poly (ethylene terephthalate) fibrous scaffold for improving cell attachment in animal cell culture被引量：10收藏
- 作者：Zhang, Weiyuan Yi, Xiaoping Sun, Xiangming Zhang, Yuanxing
- 机构：E China Univ Chem Technol
- 来源：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 2008
- 关键词：chemical modification poly (ethylene terephthalate) hydrophilicity fabric scaffold cell attachment animal cell culture
- A novel scale-up strategy for cultivation of BHK-21 cells based on similar hydrodynamic environments in the bioreactors被引量：9收藏
- 作者：Teng, Xiaonuo Li, Chao Yi, Xiaoping Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2021
- 关键词：BHK-21 cells Bioreactor Computational fluid dynamics Hydrodynamic characteristics Scale-up
- Limitations in the process of transcription and translation inhibit recombinant human chorionic gonadotropin expression in CHO cells被引量：8收藏
- 作者：Liu, Yang Yi, Xiaoping Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2015
- 关键词：Transcription limitation Recombinant protEIn Mammalian cell culture
- Functional Living Materials for Virus Disinfection Based on Engineered Bacillus subtilis Biofilm by Displaying Viral Affinity Peptides and Expressing Protease被引量：7收藏
- 作者：Zhu, Xiaojuan Chen, Jianshu Luo, Jian Liu, Xue Xu, Yan Chen, Lin Dong, Hao Zhang, Lingfan Yang, Lin Jiang, Nin Huang, Xumeng Mohsin, Ali Yi, Xiaoping Wang, Yonghong Zhuang, Yingping Zhang, Xuehong Zhang, Ke Zhang, Lixin Zhong, Chao Huang, Jiaofang
- 机构：East China Univ Sci & Technol ECUST;Shanghai Inst Biol Prod Co Ltd;Chinese Acad Sci;Ocean Univ China;East China Univ Sci & Technol
- 来源：ADVANCED FUNCTIONAL MATERIALS 2024
- 关键词：Bacillus subtilis biofilm engineered living materials SARS-CoV-2 virus disinfection
- Medium optimization based on comparative metabolomic analysis of chicken embryo fibroblast DF-1 cells被引量：5收藏
- 作者：Lin, Jia Yi, Xiaoping Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：RSC ADVANCES 2019
- 关键词：Animals - Cell culture - Metabolites - Viruses - Cell proliferation - Disease control
- A study on enhanced O-glycosylation strategy for improved production of recombinant human chorionic gonadotropin in Chinese hamster ovary cells被引量：5收藏
- 作者：Deng, Zhe Yi, Xiaoping Chu, Ju Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2019
- 关键词：Recombinant human chorionic gonadotropin CHO cell O-glycosylation GalNAc Ugp2 Galnt1
- Enhancement of hepatitis B surface antigen production by adenosine 5′-monophosphate in culture of recombinant Chinese hamster ovary cells被引量：4收藏
- 作者：Luo, Weiwen Sun, Xiangming Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2005
- 关键词：animal cell culture adenosine 5 '-monophosphate adenosine 5 '-triphosphate Chinese hamster ovary (CHO) cell hepatitis B surface antigen (HBsAg)
- 温度对重组BHK细胞生长、代谢和vWF蛋白表达的影响被引量：4收藏
- 作者：易小萍 孙祥明 张元兴
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2007
- 关键词：重组BHK细胞 细胞培养 温度 细胞周期 细胞凋亡 vWF
- 摘要：通过重组幼仓鼠肾细胞(BHK细胞)在不同温度下的批培养和脉冲培养,研究了温度对重组BHK细胞生长、代谢以及血管性血友病因子(vWF)蛋白表达的影响。相对于37℃,升高和降低温度的培养都降低了细胞的生长速率和密度,降温的作...
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- Stable over-expression of the human malate-aspartate NADH shuttle member Aralar I in PK15 cells improves energy metabolism and enhances proliferation of porcine circovirus-2被引量：3收藏
- 作者：Xia, Zhongwu Yi, Xiaoping Zhuang, Yingping
- 机构：E China Univ Sci & Technol
- 来源：RSC ADVANCES 2016
- 关键词：Amino acids - Vaccines - Viruses - Cytology - Metabolism - Physiology
- Effects of osmotic pressure on recombinant BHK cell growth and von willebrand factor (vWF) expression被引量：3收藏
- 作者：Yi, Xiaoping Sun, Xiangming Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2004
- 关键词：cell apoptosis cell cycle osmotic pressure recombinant BHK cell cell culture
- Transient gene expression of a mouse homolog of Fcε-Fcγ fusion protEIn for anti-allergic function assay被引量：1收藏
- 作者：Cheng, Lu Sun, Xiangming Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Fuchun Kexin Biotech Co Ltd
- 来源：PROCESS BIOCHEMISTRY 2011
- 关键词：Anti-allergy fusion protEIn (AAFP) Transient gene expression CHO cells PCA
- Modeling and Application of Controlled-fed Perfusion Culture of CHO Cells in a Bioreactor被引量：1收藏
- 作者：Teng, X. N. Yi, X. P. Sun, X. M. Zhang, Y. X.
- 机构：E China Univ Sci & Technol
- 来源：CHEMICAL AND BIOCHEMICAL ENGINEERING QUARTERLY 2011
- 关键词：Animal cell culture bioreactor CHO cell controlled-fed perfusion serum-free medium
- Overexpression of glutathione synthetase gene improving redox homeostasis and chicken infectious bursal disease virus propagation in chicken embryo fibroblast DF-1被引量：1收藏
- 作者：Lin, Jia Min, Rui Yi, Xiaoping Zhuang, Yingping
- 机构：East China Univ Sci & Technol ECUST;Fujian Univ Tradit Chinese Med
- 来源：BIORESOURCES AND BIOPROCESSING 2023
- 关键词：Chicken infectious bursal virus Chicken embryonic fibroblasts DF-1 Glutathione Glutathione synthetase
- Regulation of the pyruvate metabolism node by monogene and polygene engineering of HEK-293 cells被引量：0收藏
- 作者：Xie, Li Miao, Junqing Li, Xiangchao Yi, Xiaoping Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：RSC ADVANCES 2019
- 关键词：Metabolic engineering - Cell proliferation - Metabolism - Mammals - Vaccines
- Stress-induced premature senescence in high five cell cultures: a principal factor in cell-density effects被引量：0收藏
- 作者：Min, Rui Zhang, Dahe He, Mingzhe Chen, Jingyuan Yi, Xiaoping Zhuang, Yingping
- 机构：East China Univ Sci & Technol ECUST;Womei Biol Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2024
- 关键词：Cell density effect Transcriptome Proteome Time-series analysis Stress-induced premature senescence Mitochondrial dysfunction
- 表达重组蛋白的CHO-GS细胞的无血清培养被引量：0收藏
- 作者：张芳 张立 易小萍 孙祥明 张元兴
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《高技术通讯》 2005
- 关键词：重组蛋白 HADAMARD DMEM/F12 无血清培养基 谷氨酰胺 蛋白表达 细胞生长 细胞密度 统计方法 低分子量 添加浓度 外源蛋白 分批培养 CHO 多聚糖 葡聚糖 牛血清 GS 硫酸 转瓶
- 摘要：采用DOEHLERT和HADAMARD统计方法建立了一个适于重组CHO-GS细胞生长和外源蛋白表达的无谷氨酰胺无血清培养基SFMB.对多种硫酸多聚糖进行了考察,选取低分子量硫酸葡聚糖,添加浓度为25 mg/L,可以避免细...
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