成果/Result
- Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy - shaking and extra-light supplementation approach被引量：52收藏
- 作者：Li, Xu Wang, Yong-Hong Zhang, Si-Liang Chu, Ju Zhang, Ming Huang, Ming-Zhi Zhuang, Ying-Ping
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：International Journal of Hydrogen Energy 2009
- 关键词：Image enhancement - Production efficiency
- Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris被引量：45收藏
- 作者：Liu, Ping Huang, Mingzhi Guo, Menglei Qian, Jiangchao Lin, Weilu Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2016
- 关键词：Gene expression - Physiology - Recombinant protEIns - Yeast
- Machine Learning Algorithms for In-Line Monitoring During Yeast Fermentations Based on Raman Spectroscopy被引量：43收藏
- 作者：Wu, Debiao Xu, Yaying Xu, Feng Shao, Minghao Huang, Mingzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：SSRN 2023
- 关键词：Ethanol - Feature Selection - Fermentation - Glucose - Glycerol - Learning algorithms - Process control - Production efficiency - Raman spectroscopy - Spectrum analysis - Voltage measurement - Yeast
- Improved vitamin B12 production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process被引量：38收藏
- 作者：Wang, Ze-Jian Wang, Hui-Yuan Li, Yong-Liang Chu, Ju Huang, Ming-Zhi Zhuang, Ying-Ping Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering; Shijiazhuang Pharma
- 来源：Bioresource Technology 2010
- 关键词：Fermentation - Bacteria - Biosynthesis - Cell growth - Process control - Oxygen supply - Biochemistry - Growth kinetics
- Effects of light/dark cycle, mixing pattern and partial pressure of H2 on biohydrogen production by Rhodobacter sphaeroides ZX-5被引量：35收藏
- 作者：Li, Xu Wang, Yonghong Zhang, Siliang Chu, Ju Zhang, Ming Huang, Mingzhi Zhuang, Yingping
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2011
- 关键词：Fermentation - Substrates - Mixing - Partial pressure - Image enhancement
- 人工神经网络在红霉素发酵过程状态预估中的应用被引量：34收藏
- 作者：黄明志 杭海峰 储炬 叶勤 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2000
- 关键词：神经网络 BP网络 RBF网络 红霉素 发酵 状态预估
- 摘要：探索了动态 BP网络和 RBF网络在红霉素发酵过程状态预估中的应用 ,比较了它们的收敛速度和学习能力。结果表明 ,BP网络和 RBF网络都具有相当好的学习能力 ,但 RBF网络的收敛速度更快。训练好的神经网络 ,在红霉素...
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- Impacts of high β-galactosidase expression on central metabolism of recombinant Pichia pastoris GS115 using glucose as sole carbon source via 13C metabolic flux analysis被引量：31收藏
- 作者：Nie, Yongsheng Huang, Mingzhi Lu, Junjie Qian, Jiangchao Lin, Weilu Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2014
- 关键词：P. pastoris C-13 metabolic flux analysis Central metabolism Heterologous protEIn expression
- Comprehensive reconstruction and in silico analysis of Aspergillus niger genome-scale metabolic network model that accounts for 1210 ORFs被引量：28收藏
- 作者：Lu, Hongzhong Cao, Weiqiang Ouyang, Liming Xia, Jianye Huang, Mingzhi Chu, Ju Zhuang, Yingping Zhang, Siliang Noorman, Henk
- 机构：East China Univ Sci & Technol;DSM Biotechnol Ctr
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2017
- 关键词：Aspergillus niger genome-scale metabolic model multi-omics glucoamylase
- A combined strategy to simultaneously improve the component of isovalerylspiramycin in a bitespiramycin (4-O-acylspiramycins) complex and its biological titre被引量：24收藏
- 作者：Wang, Yong-Hong Xi, Bing-xia Hao, Yu-You Huang, Ming-Zhi Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2008
- 关键词：Cost effectiveness - Biochemistry
- Controlling the feed rate of glucose and propanol for the enhancement of erythromycin production and exploration of propanol metabolism fate by quantitative metabolic flux analysis被引量：22收藏
- 作者：Chen, Yong Huang, Mingzhi Wang, Zejian Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol;Qingdao Agr Univ
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2013
- 关键词：Erythromycin Propanol Metabolic flux analysis Feeding strategy
- Metabolic flux analysis of the central carbon metabolism of the industrial vitamin B12 producing strain Pseudomonas denitrificans using 13C-labeled glucose被引量：20收藏
- 作者：Wang, Ze-Jian Wang, Ping Liu, Yu-Wei Zhang, Yi-Ming Chu, Ju Huang, Ming-zhi Zhuang, Ying-Ping Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering; Microbiology Laboratory of Hebei Medical University; Department of Chemical and Biological Engineering
- 来源：Journal of the Taiwan Institute of Chemical Engineers 2012
- 关键词：Bacteria - Cultivation - Physiology - Carbon - Oxygen supply - Amino acids - Metabolism
- 柠檬酸钠对肌苷发酵过程代谢流分布的调节被引量：19收藏
- 作者：陈双喜 黄明志 储炬 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《化工学报》 2005
- 关键词：枯草杆菌 肌苷 碳源溢流 代谢流调节
- 摘要：基于物料的质量守恒定律和中间代谢物的拟稳态假设,定量研究了柠檬酸钠对肌苷发酵过程不同时段代谢流分布的影响.结果表明,添加的柠檬酸钠能够抑制存在于EMP途径和TCA循环之间的“碳源溢流”.在24h以后,EMP途径的代谢通量...
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- 13C-assisted metabolomics analysis reveals the positive correlation between specific erythromycin production rate and intracellular propionyl-CoA pool size in Saccharopolyspora erythraea被引量：19收藏
- 作者：Hong, Ming Mou, Han Liu, Xiaoyun Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Acyl-CoAs Intracellular metabolites Isotope dilution mass spectroscopy Propionyl-CoA Saccharopolyspora erythraea
- Machine learning algorithms for in-line monitoring during yeast fermentations based on Raman spectroscopy被引量：18收藏
- 作者：Wu, Debiao Xu, Yaying Xu, Feng Shao, Minghao Huang, Mingzhi
- 机构：East China Univ Sci & Technol
- 来源：VIBRATIONAL SPECTROSCOPY 2024
- 关键词：Machine learning Raman spectroscopy Yeast fermentation Process analytical technology
- Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production被引量：17收藏
- 作者：Ke, Xiang Jiang, Xing Huang, Mingzhi Tian, Xiwei Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2022
- 关键词：Saccharopolyspora erythraea Succinylation Erythromycin Aconitase CRISPRi
- Effects of vitamins (nicotinic acid, vitamin B1 and biotin) on phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5被引量：16收藏
- 作者：Li, Xu Shi, Huan Wang, Yonghong Zhang, Siliang Chu, Ju Zhang, Ming Huang, Mingzhi Zhuang, Yingping
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：International Journal of Hydrogen Energy 2011
- 关键词：Image enhancement - Vitamins - Coenzymes - Batch cell culture
- Blocking the flow of propionate into TCA cycle through a mutB knockout leads to a significant increase of erythromycin production by an industrial strain of Saccharopolyspora erythraea被引量：13收藏
- 作者：Chen, Chongchong Hong, Ming Chu, Ju Huang, Mingzhi Ouyang, Liming Tian, Xiwei Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Saccharopolyspora erythraea Erythromycin N-propanol Methylmalonyl-CoA mutase mutB
- Impacts of proline on the central metabolism of an industrial erythromycin-producing strain Saccharopolyspora erythraea via 13C labeling experiments被引量：13收藏
- 作者：Hong, Ming Huang, Mingzhi Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：C-13 metabolic flux analysis Proline Saccharopolyspora erythraea Chemically defined medium
- Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil被引量：12收藏
- 作者：Li, Jianhua Yang, Yiming Chu, Ju Huang, Mingzhi Li, Liang Zhang, Xiaochun Wang, Yonghong Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol;Shanxi Weiqida Pharmaceut Co Ltd
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2010
- 关键词：Metabolic flux analysis Acremonium chrysogenum Fed-batch cultivation Cephalosporin C
- Comprehensive reconstruction and evaluation of Pichia pastoris genome-scale metabolic model that accounts for 1243 ORFs被引量：12收藏
- 作者：Ye, Rui Huang, Mingzhi Lu, Hongzhong Qian, Jiangchao Lin, Weilu Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2017
- 关键词：Genome-scale metabolic model Pichia pastoris Multi-omics beta-Galactosidase
- In silico reconstruction and experimental validation of Saccharopolyspora erythraea genome-scale metabolic model iZZ1342 that accounts for 1685 ORFs被引量：6收藏
- 作者：Zhuang, Zhendong Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Saccharopolyspora erythraea Genome-scale metabolic model Multi-omics Erythromycin
- ATR/FT-IR监测山梨醇发酵中山梨醇和山梨糖浓度变化被引量：5收藏
- 作者：邱江 黄明志 韩崇家 叶勤 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室;华东理工大学分析测试中心
- 来源：《华东理工大学学报（自然科学版）》 1997
- 关键词：发酵 山梨醇 山梨糖 ATR/FT-IR 监测
- 摘要：采用衰减全反射傅里叶变换红外光谱（ＡＴＲ／ＦＴ－ＩＲ）技术研究了山梨醇生产山梨糖的发酵过程。确定了基质山梨醇和产物山梨糖的特征吸收峰，其特征峰的吸光度在发酵过程中的变化直接反映了山梨醇和山梨糖浓度的变化规律。建立了特征峰...
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- Ammonium sulfate supplementation enhances erythromycin biosynthesis by augmenting intracellular metabolism and precursor supply in Saccharopolyspora erythraea被引量：4收藏
- 作者：Yuan, Yujie Xu, Feng Ke, Xiang Lu, Ju Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2023
- 关键词：Saccharopolyspora erythraea Erythromycin production Ammonium sulfate Targeted metabolomics Metabolic flux analysis Cellular metabolic mechanism
- ^(13)C辅助的超高效液相色谱-三重四极杆质谱联用方法精确分析毕赤酵母胞内代谢物浓度被引量：3收藏
- 作者：郭孟磊 刘晓云 黄明志 李敏超 储炬 庄英萍 张嗣良
- 机构：华东理工大学国家生化工程技术研究中心(上海)
- 来源：《分析化学》 2016
- 关键词：超高效液相色谱-三重四极杆质谱 13C同位素标记 代谢物浓度 毕赤酵母
- 摘要：毕赤酵母作为一种高效的外源蛋白表达平台,其蛋白表达水平与胞内代谢物浓度紧密相关。但胞内代谢物种类多、物化性质差异大、浓度低、周转快,对其绝对浓度的精确检测一直难于实现。本研究将超高效液相色谱-三重四极杆质谱联用分析方法与...
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- On structural identifiability analysis of the cascaded linear dynamic systems in isotopically non-stationary 13C labelling experiments被引量：3收藏
- 作者：Lin, Weilu Wang, Zejian Huang, Mingzhi Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：MATHEMATICAL BIOSCIENCES 2018
- 关键词：Isotopically non-stationary 13C labelling experiments Structural identifiability Taylor series expansion Non-stationary cumomer balance equations
- Enhancing erythromycin production in Saccharopolyspora erythraea through rational engineering and fermentation refinement: A Design-Build-Test-Learn approach被引量：2收藏
- 作者：Shao, Minghao Xu, Feng Ke, Xiang Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2024
- 关键词：ammonium sulfate erythromycin production multi-omics analysis Saccharopolyspora erythraea systematic metabolic engineering
- 添加异戊酸对必特螺旋霉素生物合成的影响被引量：2收藏
- 作者：习兵霞 王永红 黄明志 储炬 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心(上海)
- 来源：《华东理工大学学报（自然科学版）》 2007
- 关键词：异戊酸 必特螺旋霉素 效价 发酵
- 摘要：为了进一步提高多组分必特螺旋霉素中异戊酰螺旋霉素Ⅲ和总异戊酰螺旋霉素的含量,本文通过外源添加浓度为0.5 g/L的异戊酸使异戊酰螺旋霉素Ⅲ和总异戊酰螺旋霉素的含量分别比对照提高了38.32%和31%。通过测定必特螺旋霉素...
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- Accurate Determination of Isotopic Abundance of Intracellular Metabolites of Saccharopolysporaerythraea Based on Ultra Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry被引量：1收藏
- 作者：Mou Han Hong Ming Liu Xiao-Yun Li Min-Chao Huang Ming-Zhi Chu Ju Zhuang Ying-Ping Zhang Si-Liang
- 机构：East China Univ Sci & Technol
- 来源：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 2017
- 关键词：Saccharopolysporaerythraea Intracellular metabolites C-13 isotopic abundance Ultra-high performance liquid chromatography-triple quadrupole mass spectrometry
- Modelling steady state intercellular isotopic distributions with isotopomer decomposition units被引量：1收藏
- 作者：Lin, Weilu Huang, Mingzhi Wang, Zejian Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci Technol
- 来源：COMPUTERS & CHEMICAL ENGINEERING 2019
- 关键词：Isotopomer decomposition unit Steady state isotope labeling experiments Isotopomer labeling systems Cascaded linear systems
- On stability analysis of cascaded linear time varying systems in dynamic isotope experiments被引量：0收藏
- 作者：Lin, Weilu Wang, Zejian Huang, Mingzhi Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：AICHE JOURNAL 2020
- 关键词：dynamic cumomer balance equations dynamic isotope experiments metabolic nonsteady state stability analysis
