成果/Result
- Refolding and structural characteristic of TRAIL/Apo2L inclusion bodies from different specific growth rates of recombinant Escherichia coli被引量：40收藏
- 作者：Kang, Hui Sun, Ai-You Shen, Ya-Ling Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Progress 2007
- 关键词：Fluorescence - ProtEIns - Scanning electron microscopy - Spectroscopy - Urea
- Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies被引量：33收藏
- 作者：Wang, Xiaojuan Bai, Chaogang Zhang, Jian Sun, Aiyou Wang, Xuedong Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Cell death - Metal ions - Metals - Purification - Scanning electron microscopy - Solubility
- Soluble expression, rapid purification, and characterization of human interleukin-24 (IL-24) using a MBP-SUMO dual fusion system in Escherichia coli被引量：25收藏
- 作者：Zhang, Jian Lv, Xinxin Xu, Rui Tao, Xinyi Dong, Yuguo Sun, Aiyou Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Interleukin-24 (IL-24) Small ubiquitin-related modifier (SUMO) Maltose-binding protEIn (MBP) Soluble expression One-step purification Apoptosis
- Influence of amino acids, organic solvents and surfactants for phenylalanine ammonia lyase activity in recombinant Escherichia coli被引量：17收藏
- 作者：Cui, J.D. Jia, S.R. Sun, A.Y.
- 机构： Tianjin Key Laboratory of Industry Microbiology; Tianjin Key Laboratory of Industry Microbiology; State Key Laboratory of Bioreactor Engineering
- 来源：Letters in Applied Microbiology 2008
- 关键词：Acetone - Ammonia - Enzyme activity - Escherichia coli - Organic solvents - Surface active agents
- Gigantoxin-4-4D5 scFv is a novel recombinant immunotoxin with specific toxicity against HER2/neu-positive ovarian carcinoma cells被引量：16收藏
- 作者：Lv, Xinxin Zhang, Jian Xu, Rui Dong, Yuguo Sun, Aiyou Shen, Yaling Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Immunotoxins Gigantoxin-4 HER2/neu Cancer cell targeting Ovarian carcinoma SK-OV-3 cell
- A combined feeding strategy for enhancing mycophenolic acid production by fed-batch fermentation in Penicillium brevicompactum被引量：13收藏
- 作者：Dong, Yuguo Xu, Rui Wang, Lihua Zhang, Jian Bai, Chaogang Sun, Aiyou Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2015
- 关键词：Mycophenolic acid Penicillium brevicompactum Fermentation Combined feeding strategy
- Improvement of expression level and bioactivity of soluble tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL) by a novel zinc ion feeding strategy被引量：12收藏
- 作者：Sun, Ai-You Shen, Ya-Ling Yin, Ji-Cheng Zhang, Hong Tang, Ya-Nan Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2006
- 关键词：apoptosis Escherichia coli tumor necrosis factor zinc ion feeding strategy
- TAT-RhoGDI2, a novel tumor metastasis suppressor fusion protEIn: expression, purification and functional evaluation被引量：9收藏
- 作者：Xu, Rui Dong, Yuguo Wang, Lihua Tao, Xinyi Sun, Aiyou Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：RhoGDI2 TAT-fused protEIn Purification Bladder cancer Metastasis
- Evaluation of surfactant effect on β-poly(L-malic acid) production by Aureobasidium pullulans被引量：9收藏
- 作者：Yin, Haisong Gao, Cui Ye, Kai Zhao, Tingbin Sun, Aiyou Qiao, Changsheng
- 机构：Tianjin Univ Sci & Technol;Tianjin Modern Vocat Technol Coll;Minist Educ;Tianjin Univ Sci & Technol;Tianjin Huizhi Biotrans Bioengn Co Ltd
- 来源：BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT 2019
- 关键词：Aureobasidium pullulans poly (beta-L-malic acid) Tween 80 mechanism
- Recombinant Production and Characterization of SAC, the Core Domain of Par-4, by SUMO Fusion System被引量：6收藏
- 作者：Zhang, Jian Sun, Aiyou Dong, Yuguo Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2018
- 关键词：Prostate apoptosis response-4 (Par-4) SAC Small ubiquitin-relatedmodifier (SUMO) One-step purification Ovarian cancer GRP78
- Optimisation of the mRNA secondary structure to improve the expression of interleukin-24 (IL-24) in Escherichia coli被引量：6收藏
- 作者：Bai, Chaogang Wang, Xiaojuan Zhang, Jian Sun, Aiyou Wei, Dongzhi Yang, Shengli
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2014
- 关键词：Anti-tumour Interleukin 24 Melanoma mRNA secondary structure Translation initiation region
- 透明颤菌血红蛋白基因在产TRAIL蛋白大肠杆菌中的克隆表达被引量：2收藏
- 作者：杨凌超 孙爱友 沈亚领 魏东芝 龚毅 马昱澍
- 机构：华东理工大学生物反应器工程国家重点实验室生物化学研究所;中国科学院上海生命科学研究院生物工程中心
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：透明颤菌血红蛋白基因(vgb) TRAIL 大肠杆菌 发酵 乙酸
- 摘要：为解决大肠杆菌高密度发酵生产TRA IL过程中的供氧矛盾,提高菌体的发酵密度,在大肠杆菌中引入透明颤菌血红蛋白基因(vgb)。聚合酶链反应(PCR)检验和CO差光谱法检验表明:vgb基因能够在C 600(pBV 220-...
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- 融合型乳酸片球菌素在大肠杆菌中的表达与纯化被引量：1收藏
- 作者：宋建民 孙爱友 魏东芝
- 机构：华东理工大学生物反应器工程国家重点实验室;合肥学院生物与环境工程系
- 来源：《南京理工大学学报》 2011
- 关键词：乳酸片球菌素 大肠杆菌 融合蛋白 表达 纯化
- 摘要：为在大肠杆菌中高效表达乳酸片球菌素(PED),按照美国国立生物技术信息中心公布的PED基因序列设计合成引物,以乳酸片球菌基因组DNA为模板,用多聚酶链式反应扩增PED结构基因特异片段,构建编码组氨酸标记硫氧还原蛋白融合P...
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- 根癌农杆菌介导透明颤茵血红蛋白基因(vgb)在短密青霉菌中的克隆表达被引量：0收藏
- 作者：董玉国 王丽华 徐瑞 张舰 孙爱友 魏东芝
- 机构：生物反应器工程国家重点实验室
- 来源：《高校化学工程学报》 2015
- 关键词：根癌农杆菌 短密青霉 vgb 霉酚酸 发酵
- 摘要：利用根癌农杆菌LBA4404介导,将编码透明颤菌血红蛋白的结构基因(Vitreoscilla Hemoglobin gene,vgb)导入到短密青霉ATCC16024中,用于改善菌体对氧的摄取能力,提高霉酚酸(Myhop...
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