成果/Result
- Mutant library construction in directed molecular evolution: Casting a wider net被引量：74收藏
- 作者：Wang, Tian-Wen Zhu, Hu Ma, Xing-Yuan Zhang, Ting Ma, Yu-Shu Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Molecular Biotechnology 2006
- 关键词：Cells - DNA - Enzymes - Molecular biology - Mutagenesis - Mutagens
- Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production被引量：33收藏
- 作者：Gao, Bei Su, Erzheng Lin, Jinping Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Biotechnology 2009
- 关键词：Catalysts - Cloning - Cytology - Gene encoding - Temperature - Biodiesel - Purification - Alkalinity
- Enhancing enzymatic activity of penicillin G acylase by coexpressing pcm gene被引量：30收藏
- 作者：Wang, Tianwen Zhu, Hu Ma, Xingyuan Fei, Zhuoya Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2006
- 关键词：Antibiotics - Biotechnology - Cells - Escherichia coli - Genes
- Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface被引量：26收藏
- 作者：Jiang, Zhengbing Gao, Bei Ren, Ren Tao, Xingyi Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; College of Life Science
- 来源：BMC Biotechnology 2008
- 关键词：Biocatalysts - Esters - Flocculation - Genes - Hydrolysis - Phenols - Transesterification - Yeast
- N,N-dimethylformamide induces cellulase production in the filamentous fungus Trichoderma reesEI被引量：25收藏
- 作者：Chen, Yumeng Wu, Chuan Shen, Yaling Ma, Yushu Wei, Dongzhi Wang, Wei
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY FOR BIOFUELS 2019
- 关键词：Trichoderma reesEI DMF induced Cellulase Calcium signaling Permeability Phospholipase C plc-e
- High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro被引量：23收藏
- 作者：Ma, Xingyuan Zheng, Wenyun Wei, Dongzhi Ma, Yushu Wang, Tianwen Wang, Jinzhi Liu, Qinghai Yang, Shengli
- 机构：E China Univ Sci & Technol;Shenyang Pharmaceut Univ;Chinese Acad Sci
- 来源：JOURNAL OF BIOTECHNOLOGY 2006
- 关键词：TAT-survivin (T34A) mutant high-level expression and purification pro-apoptosis
- Construction of a Novel Shuttle Vector for Use in Gluconobacter oxydans被引量：21收藏
- 作者：Zhang, Lin Lin, Jinping Ma, Yushu Wei, Dongzhi Sun, Ming
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2010
- 关键词：Shuttle vector Gluconobacter oxydans Cryptic plasmid Expression
- A simple approach to mediate genome editing in the filamentous fungus Trichoderma reesEI by CRISPR/Cas9-coupled in vivo gRNA transcription被引量：20收藏
- 作者：Wu, Chuan Chen, Yumeng Qiu, Yifei Niu, Xiao Zhu, Ningjian Chen, Jiehui Yao, Hong Wang, Wei Ma, Yushu
- 机构：East China Univ Sci & Technol;East China Normal Univ
- 来源：BIOTECHNOLOGY LETTERS 2020
- 关键词：Filamentous fungus Trichoderma reesEI CRISPR Cas9 In vivo gRNA transcription Gene editing
- A novel psychrophilic lipase from Pseudomonas fluorescens with unique property in chiral resolution and biodiesel production via transesterification被引量：19收藏
- 作者：Luo, Yu Zheng, Yitao Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2006
- 关键词：Affinity chromatography - Amino acids - Biodiesel - Catalyst activity - Escherichia coli - Gene expression - Lipases
- Analysis of the binding site of the n-terminal fragment of glucogan-like peptide-1 receptor to exendin-4被引量：19收藏
- 作者：Gao, Wei-Feng Liu, Mei-Yun Ma, Yushu
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Huadong Ligong Daxue Xuebao /Journal of East China University of Science and Technology 2008
- 新型脂肪酶LipB52催化生物柴油被引量：18收藏
- 作者：王刚 罗宇 江正兵 魏东芝 马昱澍
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：生物柴油 脂肪酶 荧光假单胞菌 转酯反应
- 摘要：一种来源于荧光假单胞菌的新型脂肪酶基因lipB52在大肠杆菌BL21(DE3)中获得表达,并且利用Ni-NTA亲和柱对脂肪酶LipB52进行纯化。采用固定化脂肪酶LipB52催化大豆油与甲醇的转酯反应生产生物柴油。实验考...
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- Expression of two old yellow enzyme homologues from Gluconobacter oxydans and identification of thEIr citral hydrogenation abilities被引量：17收藏
- 作者：Yin, Bo Yang, Xuepeng Wei, Guodong Ma, Yushu Wei, Dongzhi
- 机构： State Key Lab of Bioreactor Engineering; College of Food and Biological Engineering
- 来源：Molecular Biotechnology 2008
- 关键词：Gene expression - Cloning - Amino acids - Hydrogenation - Purification - Enzymes
- Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin被引量：17收藏
- 作者：Sun, Bing-Yao Sui, Hua-Lu Liu, Zi-Wei Tao, Xin-Yi Gao, Bei Zhao, Ming Ma, Yu-Shu Zhao, Jian Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Flavin-containing monooxygenase Indirubin Loop region Substrate tunnel Structure-guided enzyme engineering Microbial synthesis
- Display of adenoregulin with a novel Pichia pastoris cell surface display system被引量：17收藏
- 作者：Ren, Ren Jiang, Zhengbing Liu, Meiyun Tao, Xinyi Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Molecular Biotechnology 2007
- 关键词：Amino acids - Cells - Confocal microscopy - Enzyme kinetics - Flocculation - Yeast
- Production of sesquiterpene patchoulol in mitochondrion-engineered Saccharomyces cerevisiae被引量：16收藏
- 作者：Tao, Xin-Yi Lin, Yang-Chen Wang, Feng-Qing Liu, Qing-Hai Ma, Yu-Shu Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2022
- 关键词：Patchoulol Mitochondria compartmentation S cerevisiae Metabolic engineering Sesquiterpene
- Characterization of two novel lipase genes isolated directly from environmental sample被引量：13收藏
- 作者：Jiang, Zhengbing Wang, Hongping Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2006
- 关键词：Amino acids - Characterization - Environmental impact - Enzymes - Thermal effects
- Application of a silica gel prolonged-release system for methanol in the production of biodiesel被引量：11收藏
- 作者：Luo, Yu Wang, Gang Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 2006
- 关键词：biodiesel lipase prolonged release silica gel
- Characterization of a Novel NADPH-Dependent Oxidoreductase from Gluconobacter oxydans被引量：7收藏
- 作者：Chen, Minmin Lin, Jinping Ma, Yushu Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：MOLECULAR BIOTECHNOLOGY 2010
- 关键词：Gluconobacter oxydans NADPH-dependent aldehyde reductase GOX0644 ProtEIn expression Substrate specificity
- Metabolic Engineering for the Comprehensive Utilization of N,N-Dimethylformamide-Containing Wastewater被引量：3收藏
- 作者：Chen, Yumeng Wang, Meng Wang, Wei Zeng, Yi Fan, Xingjia Tu, Maobing Ma, Yushu Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Univ Cincinnati;Jiangsu YIMING Biol Technol CO LTD
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：cellulase N N-dimethylformamide separate enzymatic hydrolysis and fermentation succinate succinic acid cellulosic sugar
- 透明颤菌血红蛋白基因在产TRAIL蛋白大肠杆菌中的克隆表达被引量：2收藏
- 作者：杨凌超 孙爱友 沈亚领 魏东芝 龚毅 马昱澍
- 机构：华东理工大学生物反应器工程国家重点实验室生物化学研究所;中国科学院上海生命科学研究院生物工程中心
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：透明颤菌血红蛋白基因(vgb) TRAIL 大肠杆菌 发酵 乙酸
- 摘要：为解决大肠杆菌高密度发酵生产TRA IL过程中的供氧矛盾,提高菌体的发酵密度,在大肠杆菌中引入透明颤菌血红蛋白基因(vgb)。聚合酶链反应(PCR)检验和CO差光谱法检验表明:vgb基因能够在C 600(pBV 220-...
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- Cloning and expression of a novel lipase gene from Pseudomonas fluorescens B52被引量：0收藏
- 作者：Jiang, Zhengbing Zheng, Yitao Luo, Yu Wang, Gang Wang, Hongping Ma, Yushu Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Molecular Biotechnology 2005
- 关键词：DNA - Fluorescence - Fungi - Genes
- Production and purification of a novel antibiotic peptide, adenoregulin, from a recombinant Escherichia coli被引量：0收藏
- 作者：Zhou, Yu-Xun Cao, Wei Luo, Qing-Ping Ma, Yu-Shu Wang, Jin-Zhi Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Letters 2005
- 关键词：Bacteria - Cultivation - Escherichia coli - Fungi - ProtEIns - Protozoa
- Rational engineering of Corynebacterium glutamicum for L-Glutamine biosynthesis被引量：0收藏
- 作者：Lin, Ying-Tong Chai, Meng Liu, Qing-Hai Ma, Yu-Shu Tao, Xin-Yi Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2026
- 关键词：L-Glutamine Metabolic engineering RBS engineering SRNA interference Growth-responsive promoter
- B淋巴细胞刺激因子基因的克隆及在大肠杆菌中的高效表达被引量：0收藏
- 作者：王锦之 周卿 周宇荀 马昱澍 魏东芝
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2005
- 关键词：B淋巴细胞刺激因子 大肠杆菌 克隆和表达 丝裂霉素 协同作用
- 摘要：聚合酶链式反应(PCR)扩增获得了编码BLys(134-285AA)的cDNA片段,该片段以限制性内切酶Bgl 和Hind 双酶切插入pET32a载体。测序表明除(ATA263→ATG263)突变并导致了氨基酸(I263...
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- Metabolic engineering of Escherichia coli for efficient production of O-succinyl-L-homoserine被引量：0收藏
- 作者：Liu, Longmei Gu, Jieyun Lyu, Xiaomei Gu, JiaLi Liu, Qinghai Ma, Yu-Shu Tao, Xin-Yi Liu, Min Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Jiangnan Univ;Huzhou Univ
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2026
- 关键词：O-Succinyl-L-homoserine Escherichia coli Self-regulating promoter Metabolic engineering Flagellar system
