成果/Result
- The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction被引量：194收藏
- 作者：Liu, Guo-Song Li, Tian Zhou, Wei Jiang, Min Tao, Xin-Yi Liu, Min Zhao, Ming Ren, Yu-Hong Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2020
- 关键词：Synthetic biology Saccharomyces cerevisiae Peroxisome Storage depot Subcellular factory Terpene
- An improved 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reduction assay for evaluating the viability of Escherichia coli cells被引量：181收藏
- 作者：Wang, Hengwei Cheng, Hairong Wang, Fengqing Wei, Dongzhi Wang, Xuedong
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：JOURNAL OF MICROBIOLOGICAL METHODS 2010
- 关键词：MTT reduction Formazan E. coli Viability
- Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction被引量：115收藏
- 作者：Zhu, Zhan-Tao Du, Meng-Meng Gao, Bei Tao, Xin-Yi Zhao, Ming Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2021
- 关键词：Saccharomyces cerevisiae Mitochondrial engineering Metabolic burden Squalene Dual engineering
- Characterization and engineering of 3-ketosteroid-Δ1-dehydrogenase and 3-ketosteroid-9α-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9α-hydroxy-4-androstene-3,17-dione through the catabolism of sterols被引量：99收藏
- 作者：Yao, Kang Xu, Li-Qin Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2014
- 关键词：3-Ketosteroid-Delta(1)-dehydrogenase Mycobacteria Sterols 9 alpha-Hydroxy-4-androstene-3,17-dione
- Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum被引量：93收藏
- 作者：Yao, Kang Wang, Feng-Qing Zhang, Huai-Cheng Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2013
- 关键词：Cholesterol oxidase Mycobacteria Sterols Androst-4-ene-3,17-dione Androst-1,4-diene-3,17-dione
- Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism被引量：80收藏
- 作者：Xu, Li-Qin Liu, Yong-Jun Yao, Kang Liu, Hao-Hao Tao, Xin-Yi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：SCIENTIFIC REPORTS 2016
- Metabolic Engineering of Saccharomyces cerevisiae To Overproduce Squalene被引量：71收藏
- 作者：Li, Tian Liu, Guo-Song Zhou, Wei Jiang, Min Ren, Yu-Hong Tao, Xin-Yi Liu, Min Zhao, Ming Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Saccharomyces cerevisiae metabolic engineering squalene overproduction ethanol carbon source utilization
- Inactivation and Augmentation of the Primary 3-Ketosteroid-Δ1-Dehydrogenase in Mycobacterium neoaurum NwIB-01: Biotransformation of Soybean Phytosterols to 4-Androstene-3,17-Dione or 1,4-Androstadiene-3,17-Dione被引量：63收藏
- 作者：Wei, Wei Wang, Feng-qing Fan, Shu-yue Wei, Dong-zhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2010
- High-Level Production of Sesquiterpene Patchoulol in Saccharomyces cerevisiae被引量：57收藏
- 作者：Liu, Min Lin, Yang-Chen Guo, Jiao-Jiao Du, Meng-Meng Tao, Xinyi Gao, Bei Zhao, Ming Ma, Yushu Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2021
- 关键词：patchoulol physiological status metabolic engineering Saccharomyces cerevisiae sesquiterpene cell factories
- Significantly Enhanced Production of Patchoulol in Metabolically Engineered Saccharomyces cerevisiae被引量：53收藏
- 作者：Ma, Bin Liu, Min Li, Zhen-Hai Tao, Xinyi Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：patchoulol sesquiterpene metabolic engineering Saccharomyces cerevisiae terpenoids
- Ammonia-containing dimethyl sulfoxide: An improved solvent for the dissolution of formazan crystals in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay被引量：51收藏
- 作者：Wang, Hengwei Wang, Fengqing Tao, Xinyi Cheng, Hairong
- 机构：Shanghai Jiao Tong Univ;E China Univ Sci & Technol
- 来源：ANALYTICAL BIOCHEMISTRY 2012
- 关键词：MTT assay Formazan Dimethyl sulfoxide Ammonia pH Viability
- Metabolic engineering of Pichia pastoris for the production of dammarenediol-II被引量：51收藏
- 作者：Liu, Xin-Bin Liu, Min Tao, Xin-Yi Zhang, Zhong-Xi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2015
- 关键词：Dammarenediol-II Metabolic engineering Pichia pastoris Ginsenosides 2,3-Oxidosqualene
- Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurum被引量：45收藏
- 作者：Xiong, Liang-Bin Liu, Hao-Hao Zhao, Ming Liu, Yong-Jun Song, Lu Xie, Zhi-Yong Xu, Yi-Xin Wang, Feng-Qing Wei, Dong-Zhi
- 机构：Shanghai Univ Med & Hlth Sci;East China Univ Sci & Technol;Shanghai Univ Med & Hlth Sci
- 来源：MICROBIAL CELL FACTORIES 2020
- 关键词：Mycolic acids Self-enhancement conversion beta-Ketoacyl-acyl carrier protein synthase Phytosterol Steroid intermediates
- The MrCYP52 Cytochrome P450 Monoxygenase Gene of Metarhizium robertsii Is Important for Utilizing Insect Epicuticular Hydrocarbons被引量：41收藏
- 作者：Lin, Liangcai Fang, Weiguo Liao, Xinggang Wang, Fengqing Wei, Dongzhi Leger, Raymond J. St.
- 机构：E China Univ Sci & Technol;Univ Maryland
- 来源：PLOS ONE 2011
- Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes被引量：38收藏
- 作者：Xiong, Liang-Bin Liu, Hao-Hao Xu, Li-Qin Sun, Wan-Ju Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2017
- 关键词：Mycobacterium 22-Hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC) mmpL3 choM cyp125 fadA5 Sterol catabolism
- Enhancing Biosynthesis of a Ginsenoside Precursor by Self-Assembly of Two Key Enzymes in Pichia pastoris被引量：36收藏
- 作者：Zhao, Chengcheng Gao, Xin Liu, Xinbin Wang, Yong Yang, Shengli Wang, Fengqing Ren, Yuhong
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2016
- 关键词：ginsenosides self-assembly Pichia pastoris dammarenediol-II
- Engineered 3-Ketosteroid 9α-Hydroxylases in Mycobacterium neoaurum: an Efficient Platform for Production of Steroid Drugs被引量：36收藏
- 作者：Liu, Hao-Hao Xu, Li-Qin Yao, Kang Xiong, Liang-Bin Tao, Xin-Yi Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2018
- 关键词：3-ketosteroid-9 alpha-hydroxylase androst-1,4-diene-3,17-dione (ADD) 9 alpha-hydroxy-4-androstene-3,17-dione (9-OHAD) mycobacterium Mn25795 sterols 9 alpha-hydroxy-4-androstene-3,17-dione androst-1,4-diene-3,17-dione sterol
- Improving the Expression of Recombinant Proteins in E. coli BL21 (DE3) under Acetate Stress: An Alkaline pH Shift Approach被引量：35收藏
- 作者：Wang, Hengwei Wang, Fengqing Wang, Wei Yao, Xueling Wei, Dongzhi Cheng, Hairong Deng, Zixin
- 机构：Zhejiang Ocean Univ;Shanghai Jiao Tong Univ;E China Univ Sci & Technol
- 来源：PLOS ONE 2014
- Engineering Saccharomyces cerevisiae for Hyperproduction of β-Amyrin by Mitigating the Inhibition Effect of Squalene on β-Amyrin Synthase被引量：35收藏
- 作者：Du, Meng-Meng Zhu, Zhan-Tao Zhang, Ge-Ge Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Liu, Min Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：beta-amyrin squalene inhibition peroxisome compartmentalization competition pathway Saccharomyces cerevisiae
- Biotransformation of diosgenin to nuatigenin-type steroid by a newly isolated strain, Streptomyces virginiae IBL-14被引量：31收藏
- 作者：Wang, Feng-Qing Li, Bo Wang, Wei Zhang, Cheng-Gang Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2007
- 关键词：diosgenin diosgenone isonuatigenone nuatigenin-type steroid tertiary hydroxylation Streptomyces virginiae
- Role Identification and Application of SigD in the Transformation of Soybean Phytosterol to 9α-Hydroxy-4-androstene-3,17-dione in Mycobacterium neoaurum被引量：30收藏
- 作者：Xiong, Liang-Bin Liu, Hao-Hao Xu, Li-Qin Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：mycobacteria 9a-hydroxy-4-androstene-3,17-dione (9-OHAD) soybean phytosterol transcription factor sigD choM2
- Characterization and engineering control of the effects of reactive oxygen species on the conversion of sterols to steroid synthons in Mycobacterium neoaurum被引量：29收藏
- 作者：Sun, Wan-Ju Wang, Li Liu, Hao-Hao Liu, Yong-Jun Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Sterol catabolism Reactive oxygen species Catalase Mycothiol Ergothioneine Mycobacteria
- Accumulation of androstadiene-dione by overexpression of heterologous 3-ketosteroid Δ1-dehydrogenase in Mycobacterium neoaurum NwIB-01被引量：29收藏
- 作者：Wei, Wei Fan, Shu-Yue Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：1,4-Androstadiene-3,17-dione 3-Ketosteroid Delta(1)-dehydrogenase 4-Androstene-3,17-dione Gene overexpression Phytosterols Plasmid stability
- Enhanced itaconic acid production by self-assembly of two biosynthetic enzymes in Escherichia coli被引量：27收藏
- 作者：Yang, Zhongwei Gao, Xin Xie, Hui Wang, Fengqing Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2017
- 关键词：itaconic acid aconitase cis-aconitate decarboxylase self-assembly protein-peptide interactions
- Novel Application of Magnetic Protein: Convenient One-Step Purification and Immobilization of Proteins被引量：27收藏
- 作者：Jiang, Min Zhang, Lujia Wang, Fengqing Zhang, Jie Liu, Guosong Gao, Bei Wei, Dongzhi
- 机构：East China Univ Sci & Technol;East China Normal Univ;NYU Shanghai;East China Normal Univ
- 来源：SCIENTIFIC REPORTS 2017
- A comparative study of β-1, 4-endoglucanase (possessing β-1, 4-exoglucanase activity) from Bacillus subtilis LH expressed in Pichia pastoris GS115 and Escherichia coli Rosetta (DE3)被引量：27收藏
- 作者：Zhao, Xi-Hua Wang, Wei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol;Jiangxi Normal Univ
- 来源：BIORESOURCE TECHNOLOGY 2012
- 关键词：beta-1, 4-Endoglucanase Escherichia coli Rosetta (DE3) Pichia pastoris GS115 Glycosylation Biostoning
- Characterization and application of fusidane antibiotic biosynethsis enzyme 3-ketosteroid-Δ1-dehydrogenase in steroid transformation被引量：25收藏
- 作者：Chen, Miao-Miao Wang, Feng-Qing Lin, Liang-Cai Yao, Kang Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2012
- 关键词：3-Ketosteroid-Delta(1)-dehydrogenase Steroidal antibiotic fusidanes Steroid transformation 4-Androstene-3,17-dione 1,4-Androstadiene-3,17-dione Boldenone
- Increasing L-homoserine production in Escherichia coli by engineering the central metabolic pathways被引量：25收藏
- 作者：Liu, Min Lou, Jiali Gu, Jiali Lyu, Xiao-Mei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Huzhou Univ;Nanyang Technol Univ
- 来源：JOURNAL OF BIOTECHNOLOGY 2020
- 关键词：L-Homoserine Central metabolic pathways Metabolic engineering Escherichia coli
- Integrated Transcriptome and Proteome Studies Reveal the Underlying Mechanisms for Sterol Catabolism and Steroid Production in Mycobacterium neoaurum被引量：24收藏
- 作者：Liu, Min Xiong, Liang-Bin Tao, Xinyi Liu, Qing-Hai Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：sterol catabolism transcriptome proteome Mycobacterium
- A New Steroid-Transforming Strain of Mycobacterium neoaurum and Cloning of 3-Ketosteroid 9α-Hydroxylase in NwIB-01被引量：23收藏
- 作者：Wei, Wei Fan, Shuyue Wang, Fengqing Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Mycobacterium neoaurum Extraction chromatography Screening Phytosterols 4-Androstene-3,17-dione (AD) 1,4-Androstadiene-3,17-dione (ADD) 3-Ketosteroid 9 alpha-hydroxylase (KSH)
