成果/Result
- Improved vitamin B12 production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process被引量：74收藏
- 作者：Wang, Ze-Jian Wang, Hui-Yuan Li, Yong-Liang Chu, Ju Huang, Ming-Zhi Zhuang, Ying-Ping Zhang, Si-Liang
- 机构：E China Univ Sci & Technol;Grp Hua Rong Pharmaceut Co Ltd
- 来源：BIORESOURCE TECHNOLOGY 2010
- 关键词：Vitamin B-12 Oxygen uptake rate Pseudomonas denitrificans Scale-up Oxygen supply strategy
- Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy - shaking and extra-light supplementation approach被引量：62收藏
- 作者：Li, Xu Wang, Yong-Hong Zhang, Si-Liang Chu, Ju Zhang, Ming Huang, Ming-Zhi Zhuang, Ying-Ping
- 机构：E China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 2009
- 关键词：Photo-hydrogen production Rhodobacter sphaeroides Light intensity Hydrogen production rate Light attenuation kinetics
- Effects of light/dark cycle, mixing pattern and partial pressure of H2 on biohydrogen production by Rhodobacter sphaeroides ZX-5被引量：47收藏
- 作者：Li, Xu Wang, Yonghong Zhang, Siliang Chu, Ju Zhang, Ming Huang, Mingzhi Zhuang, Yingping
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2011
- 关键词：Photo-hydrogen production Rhodobacter sphaeroides Light/dark cycle Shaking velocity Hydrogen partial pressure
- Multi-omics integrative analysis with genome-scale metabolic model simulation reveals global cellular adaptation of Aspergillus niger under industrial enzyme production condition被引量：36收藏
- 作者：Lu, Hongzhong Cao, Weiqiang Liu, Xiaoyun Sui, Yufei Ouyang, Liming Xia, Jianye Huang, Mingzhi Zhuang, Yingping Zhang, Siliang Noorman, Henk Chu, Ju
- 机构：East China Univ Sci & Technol;DSM Biotechnol Ctr
- 来源：SCIENTIFIC REPORTS 2018
- Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger被引量：35收藏
- 作者：Lu, Hongzhong Liu, Xiaoyun Huang, Mingzhi Xia, Jianye Chu, Ju Zhuang, Yingping Zhang, Siliang Noorman, Henk
- 机构：E China Univ Sci & Technol;DSM Biotechnol Ctr
- 来源：MICROBIAL CELL FACTORIES 2015
- 关键词：Aspergillus niger Glucoamylase C-13 metabolic flux analysis Metabolomics Cofactor metabolism
- Impacts of high β-galactosidase expression on central metabolism of recombinant Pichia pastoris GS115 using glucose as sole carbon source via 13C metabolic flux analysis被引量：31收藏
- 作者：Nie, Yongsheng Huang, Mingzhi Lu, Junjie Qian, Jiangchao Lin, Weilu Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2014
- 关键词：P. pastoris C-13 metabolic flux analysis Central metabolism Heterologous protein expression
- Mixomics analysis of Bacillus subtilis: effect of oxygen availability on riboflavin production被引量：28收藏
- 作者：Hu, Junlang Lei, Pan Mohsin, Ali Liu, Xiaoyun Huang, Mingzhi Li, Liang Hu, Jianhua Hang, Haifeng Zhuang, Yingping Guo, Meijin
- 机构：East China Univ Sci & Technol;Shanghai Acebright Pharmaceut Grp Co Ltd
- 来源：MICROBIAL CELL FACTORIES 2017
- 关键词：Riboflavin Bacillus subtilis Oxygen availability C-13 metabolic flux analysis Metabolomics Transcriptomics
- Comprehensive reconstruction and in silico analysis of Aspergillus niger genome-scale metabolic network model that accounts for 1210 ORFs被引量：28收藏
- 作者：Lu, Hongzhong Cao, Weiqiang Ouyang, Liming Xia, Jianye Huang, Mingzhi Chu, Ju Zhuang, Yingping Zhang, Siliang Noorman, Henk
- 机构：East China Univ Sci & Technol;DSM Biotechnol Ctr
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2017
- 关键词：Aspergillus niger genome-scale metabolic model multi-omics glucoamylase
- Controlling the feed rate of glucose and propanol for the enhancement of erythromycin production and exploration of propanol metabolism fate by quantitative metabolic flux analysis被引量：22收藏
- 作者：Chen, Yong Huang, Mingzhi Wang, Zejian Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol;Qingdao Agr Univ
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2013
- 关键词：Erythromycin Propanol Metabolic flux analysis Feeding strategy
- 13C-assisted metabolomics analysis reveals the positive correlation between specific erythromycin production rate and intracellular propionyl-CoA pool size in Saccharopolyspora erythraea被引量：19收藏
- 作者：Hong, Ming Mou, Han Liu, Xiaoyun Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Acyl-CoAs Intracellular metabolites Isotope dilution mass spectroscopy Propionyl-CoA Saccharopolyspora erythraea
- Machine learning algorithms for in-line monitoring during yeast fermentations based on Raman spectroscopy被引量：18收藏
- 作者：Wu, Debiao Xu, Yaying Xu, Feng Shao, Minghao Huang, Mingzhi
- 机构：East China Univ Sci & Technol
- 来源：VIBRATIONAL SPECTROSCOPY 2024
- 关键词：Machine learning Raman spectroscopy Yeast fermentation Process analytical technology
- Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production被引量：17收藏
- 作者：Ke, Xiang Jiang, Xing Huang, Mingzhi Tian, Xiwei Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2022
- 关键词：Saccharopolyspora erythraea Succinylation Erythromycin Aconitase CRISPRi
- Exploring the metabolic fate of propanol in industrial erythromycin-producing strain via 13C labeling experiments and enhancement of erythromycin production by rational metabolic engineering of Saccharopolyspora erythraea被引量：16收藏
- 作者：Xu, Feng Ke, Xiang Hong, Ming Huang, Mingzhi Chen, Chongchong Tian, Xiwei Hang, Haifeng Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2021
- 关键词：Saccharopolyspora erythraea Erythromycin Metabolic flux analysis N-Propanol Succinyl-CoA synthetase
- Blocking the flow of propionate into TCA cycle through a mutB knockout leads to a significant increase of erythromycin production by an industrial strain of Saccharopolyspora erythraea被引量：13收藏
- 作者：Chen, Chongchong Hong, Ming Chu, Ju Huang, Mingzhi Ouyang, Liming Tian, Xiwei Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Saccharopolyspora erythraea Erythromycin N-propanol Methylmalonyl-CoA mutase mutB
- Impacts of proline on the central metabolism of an industrial erythromycin-producing strain Saccharopolyspora erythraea via 13C labeling experiments被引量：13收藏
- 作者：Hong, Ming Huang, Mingzhi Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：C-13 metabolic flux analysis Proline Saccharopolyspora erythraea Chemically defined medium
- Effects of vitamins (nicotinic acid, vitamin B1 and biotin) on phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5被引量：13收藏
- 作者：Li, Xu Shi, Huan Wang, Yonghong Zhang, Siliang Chu, Ju Zhang, Ming Huang, Mingzhi Zhuang, Yingping
- 机构：E China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 2011
- 关键词：Photo-hydrogen production Rhodobacter sphaeroides Vitamin Nicotinic acid NAD(+)/NADH
- Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil被引量：12收藏
- 作者：Li, Jianhua Yang, Yiming Chu, Ju Huang, Mingzhi Li, Liang Zhang, Xiaochun Wang, Yonghong Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol;Shanxi Weiqida Pharmaceut Co Ltd
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2010
- 关键词：Metabolic flux analysis Acremonium chrysogenum Fed-batch cultivation Cephalosporin C
- Reconstruction of the Genome-Scale Metabolic Model of Saccharopolyspora erythraea and Its Application in the Overproduction of Erythromycin被引量：12收藏
- 作者：Xu, Feng Lu, Ju Ke, Xiang Shao, Minghao Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：METABOLITES 2022
- 关键词：genome-scale metabolic model Saccharopolyspora erythraea iJL1426 erythromycin production n-propanol process optimization
- Towards a hybrid model-driven platform based on flux balance analysis and a machine learning pipeline for biosystem design被引量：10收藏
- 作者：Wu, Debiao Xu, Feng Xu, Yaying Huang, Mingzhi Li, Zhimin Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2024
- 关键词：Metabolic modeling Machine learning Flux balance analysis Biosystems design Saccharomyces cerevisiae Succinate dehydrogenase
- Metabolic flux analysis of the central carbon metabolism of the industrial vitamin B12 producing strain Pseudomonas denitrificans using 13C-labeled glucose被引量：7收藏
- 作者：Wang, Ze-Jian Wang, Ping Liu, Yu-Wei Zhang, Yi-Ming Chu, Ju Huang, Ming-zhi Zhuang, Ying-Ping Zhang, Si-Liang
- 机构：E China Univ Sci & Technol;Hebei Med Univ;Chalmers
- 来源：JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS 2012
- 关键词：C-13 metabolic network analysis Vitamin B-12 Pseudomonas denitrificans Oxygen limiting level
- In silico reconstruction and experimental validation of Saccharopolyspora erythraea genome-scale metabolic model iZZ1342 that accounts for 1685 ORFs被引量：6收藏
- 作者：Zhuang, Zhendong Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Saccharopolyspora erythraea Genome-scale metabolic model Multi-omics Erythromycin
- Untargeted Metabolomics Combined with Metabolic Flux Analysis Reveals the Mechanism of Sodium Citrate for High S-Adenosyl-Methionine Production by Pichia pastoris被引量：5收藏
- 作者：Xu, Wentao Xu, Feng Song, Weijing Dong, Le Qian, Jiangchao Huang, Mingzhi
- 机构：East China Univ Sci & Technol
- 来源：FERMENTATION-BASEL 2022
- 关键词：Pichia pastoris S-adenosyl-methionine sodium citrate untargeted metabolomics metabolic flux analysis
- Accurate Determination of 13C Isotopic Abundance of Free Intracellular Amino acids with Low Concentration by GC-MS-Selective Ion Monitoring Method被引量：4收藏
- 作者：Li Min-Chao Huang Ming-Zhi Liu Yu-Wei Chu Ju Zhuang Ying-Ping Zhang Si-Liang
- 机构：E China Univ Sci & Technol
- 来源：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 2014
- 关键词：Selected ion monitoring method C-13 isotopic abundance Gas chromatography-mass spectroscopy Free amino acids
- Ammonium sulfate supplementation enhances erythromycin biosynthesis by augmenting intracellular metabolism and precursor supply in Saccharopolyspora erythraea被引量：4收藏
- 作者：Yuan, Yujie Xu, Feng Ke, Xiang Lu, Ju Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2023
- 关键词：Saccharopolyspora erythraea Erythromycin production Ammonium sulfate Targeted metabolomics Metabolic flux analysis Cellular metabolic mechanism
- ^(13)C辅助的超高效液相色谱-三重四极杆质谱联用方法精确分析毕赤酵母胞内代谢物浓度被引量：3收藏
- 作者：郭孟磊 刘晓云 黄明志 李敏超 储炬 庄英萍 张嗣良
- 机构：华东理工大学国家生化工程技术研究中心(上海)
- 来源：《分析化学》 2016
- 关键词：超高效液相色谱-三重四极杆质谱 13C同位素标记 代谢物浓度 毕赤酵母
- 摘要：毕赤酵母作为一种高效的外源蛋白表达平台,其蛋白表达水平与胞内代谢物浓度紧密相关。但胞内代谢物种类多、物化性质差异大、浓度低、周转快,对其绝对浓度的精确检测一直难于实现。本研究将超高效液相色谱-三重四极杆质谱联用分析方法与...
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- A combined strategy to simultaneously improve the component of isovalerylspiramycin in a bitespiramycin (4"-O-acylspiramycins) complex and its biological titre被引量：3O-acylspiramycins) complex and its biological titre')">收藏
- 作者：Wang, Yong-Hong Xi, Bing-xia Hao, Yu-You Huang, Ming-Zhi Zhang, Si-Liang
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2008
- 关键词：Streptomyces bitespiramycin isovalerate 2-oxoglutarate composition
- On structural identifiability analysis of the cascaded linear dynamic systems in isotopically non-stationary 13C labelling experiments被引量：3收藏
- 作者：Lin, Weilu Wang, Zejian Huang, Mingzhi Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：MATHEMATICAL BIOSCIENCES 2018
- 关键词：Isotopically non-stationary 13C labelling experiments Structural identifiability Taylor series expansion Non-stationary cumomer balance equations
- Enhancing erythromycin production in Saccharopolyspora erythraea through rational engineering and fermentation refinement: A Design-Build-Test-Learn approach被引量：2收藏
- 作者：Shao, Minghao Xu, Feng Ke, Xiang Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2024
- 关键词：ammonium sulfate erythromycin production multi-omics analysis Saccharopolyspora erythraea systematic metabolic engineering
- Accurate Determination of Isotopic Abundance of Intracellular Metabolites of Saccharopolysporaerythraea Based on Ultra Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry被引量：1收藏
- 作者：Mou Han Hong Ming Liu Xiao-Yun Li Min-Chao Huang Ming-Zhi Chu Ju Zhuang Ying-Ping Zhang Si-Liang
- 机构：East China Univ Sci & Technol
- 来源：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 2017
- 关键词：Saccharopolysporaerythraea Intracellular metabolites C-13 isotopic abundance Ultra-high performance liquid chromatography-triple quadrupole mass spectrometry
- Modelling steady state intercellular isotopic distributions with isotopomer decomposition units被引量：1收藏
- 作者：Lin, Weilu Huang, Mingzhi Wang, Zejian Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci Technol
- 来源：COMPUTERS & CHEMICAL ENGINEERING 2019
- 关键词：Isotopomer decomposition unit Steady state isotope labeling experiments Isotopomer labeling systems Cascaded linear systems
