成果/Result
- Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol被引量：123收藏
- 作者：Liu, Yiqi Tu, Xiaohu Xu, Qin Bai, Chenxiao Kong, Chuixing Liu, Qi Yu, Jiahui Peng, Qiangqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Methanol Methylotrophic yeast Co-culture Heterologous biosynthesis Monacolin J Lovastatin
- CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris被引量：89收藏
- 作者：Liu, Qi Shi, Xiaona Song, Lili Liu, Haifeng Zhou, Xiangshan Wang, Qiyao Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Chinare Resources Angde Biotech Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：MICROBIAL CELL FACTORIES 2019
- 关键词：Pichia pastoris CRISPR-Cas9 Homology directed repair Multiloci integration Multistep enzymatic pathway
- Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast被引量：45收藏
- 作者：Liu, Yiqi Bai, Chenxiao Liu, Qi Xu, Qin Qian, Zhilan Peng, Qiangqiang Yu, Jiahui Xu, Mingqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Ethanol Crabtree-negative yeast Acetyl-CoA Monacolin J Metabolic engineering Coculture
- A programmable high-expression yeast platform responsive to user-defined signals被引量：35收藏
- 作者：Liu, Qi Song, Lili Peng, Qiangqiang Zhu, Qiaoyun Shi, Xiaona Xu, Mingqiang Wang, Qiyao Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SCIENCE ADVANCES 2022
- 关键词：Recombinant proteins - Transcription
- de Novo Production of Plant 4′-deoxyflavones Baicalein and Oroxylin A from Ethanol in Crabtree-Negative Yeast被引量：32收藏
- 作者：Qian, Zhilan Yu, Jiahui Chen, Xinjie Kang, Yijia Ren, Yanna Liu, Qi Lu, Jian Zhao, Qing Cai, Menghao
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Chinese Acad Sci;East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：plant 4 '-deoxyflavones Scutellaria baicalensis Georgi Pichia pastoris transcriptional regulation device fine-tuning pathway control
- Synthesis of core-shell molecularly imprinted polymers (MIP) for spiramycin I and their application in MIP chromatography被引量：29收藏
- 作者：Liu, Qi Wan, Junfen Cao, Xuejun
- 机构：East China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2018
- 关键词：Spiramycin I Core-shell molecularly imprinted polymers Selectivity Chromatographic column
- Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis被引量：26收藏
- 作者：Kong, Chuixing Huang, Hezhou Xue, Ying Liu, Yiqi Peng, Qiangqiang Liu, Qi Xu, Qin Zhu, Qiaoyun Yin, Ying Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SCIENTIFIC REPORTS 2018
- Coproduction of single cell protein and lipid from lignocellulose derived carbohydrates and inorganic ammonia salt with soluble ammonia recycling被引量：26收藏
- 作者：Zhang, Bin Ren, Dayu Liu, Qi Liu, Xiucai Bao, Jie
- 机构：East China Univ Sci & Technol;Cathay Biotech Inc
- 来源：BIORESOURCE TECHNOLOGY 2023
- 关键词：Single cell protein Microbial lipid Lignocellulose Trichosporon cutaneum Wastewater
- Ultra-centrifugation force in adaptive evolution changes the cell structure of oleaginous yeast Trichosporon cutaneum into a favorable space for lipid accumulation被引量：16收藏
- 作者：Liu, Qi Lu, Minping Jin, Ci Hou, Weiliang Zhao, Liao Bao, Jie
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2022
- 关键词：adaptive evolution cell structure lignocellulose microbial lipid oleaginous yeast ultra-centrifugation
- Engineered dynamic distribution of malonyl-CoA flux for improving polyketide biosynthesis in Komagataella phaffii被引量：15收藏
- 作者：Wen, Jiao Tian, Lin Liu, Qi Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：JOURNAL OF BIOTECHNOLOGY 2020
- 关键词：Komagataella phaffii FapR/fapO Malonyl-CoA Dynamic control Synthetic biology
- Engineering substrate and energy metabolism for living cell production of cytidine-5′-diphosphocholine被引量：12收藏
- 作者：Ren, Yanna Liu, Qi Liu, Haifeng Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;China Resources Angde Biotech Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2020
- 关键词：ATP generation choline transport cytidine-5 '-diphosphocholine metabolic engineering Pichia pastoris
- de novo transcriptome sequencing of marine-derived Aspergillus glaucus and comparative analysis of metabolic and developmental variations in response to salt stress被引量：12收藏
- 作者：Liu, Shaomei Li, Jiaxin Wu, Yuan Ren, Yanna Liu, Qi Wang, Qiyao Zhou, Xiangshan Cai, Menghao Zhang, Yuanxing
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：GENES & GENOMICS 2017
- 关键词：Marine fungi Aspergillus glaucus Salt stress RNA-Seq Asexual development Secondary metabolites
- Yeast transcriptional device libraries enable precise synthesis of value-added chemicals from methanol被引量：12收藏
- 作者：Zhu, Qiaoyun Liu, Qi Yao, Chaoying Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;East China Univ Sci & Technol
- 来源：NUCLEIC ACIDS RESEARCH 2022
- Cellulase mediated stress triggers the mutations of oleaginous yeast Trichosporon cutaneum with super-large spindle morphology and high lipid accumulation被引量：8收藏
- 作者：Liu, Qi Li, Yuanyuan Hou, Weiliang Zhang, Bin Bao, Jie
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2023
- 关键词：cell size cell wall structure cellulase microbial lipid oleaginous yeast
- High-level living cell production of cytidine-5′-diphosphocholine in metabolically engineered yeast被引量：7收藏
- 作者：Ren, Yanna Liu, Qi Liu, Haifeng Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;China Resources Angde Biotech Pharma Co Ltd;China Resources Biopharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：JOURNAL OF BIOTECHNOLOGY 2021
- 关键词：Living cell production Metabolic engineering Cytidine-5 '-diphosphocholine Pichia pastoris
- Simultaneous enhancement of lignin-derived inhibitor tolerance and lipid accumulation of oleaginous yeast Trichosporon cutaneum by adaptive evolution被引量：7收藏
- 作者：Liu, Qi Zhang, Bin Hu, Mingshan Bao, Jie
- 机构：East China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2024
- 关键词：Lignocellulose Biolipid Oleaginous yeast Inhibitors Adaptive evolution
- de novo biosynthesis and nicotinamide biotransformation of nicotinamide mononucleotide by engineered yeast cells被引量：7收藏
- 作者：Ren, Yanna Han, Bei Wang, Shijie Wang, Xingbin Liu, Qi Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met
- 来源：MICROBIAL BIOTECHNOLOGY 2024
- Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris被引量：6收藏
- 作者：Liu, Qi He, Ziyu Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2025
- 关键词：Pichia pastoris Methanol metabolism Transcriptome analysis Transcription activator AOX1 promoter
- Recycling of waste calcium carbonate in lignocellulosic biorefining chain for chiral lactic acid production被引量：4收藏
- 作者：Zhang, Bin Han, Qingmei Liu, Qi Wang, Ya Bao, Jie
- 机构：East China Univ Sci & Technol;Shihezi Univ
- 来源：BIORESOURCE TECHNOLOGY 2024
- 关键词：Lignocellulose Biorefinery Solid residues Ash Neutralization
- Nucleotide distribution analysis of 5′UTRs in genome-scale directs their redesign and expression regulation in yeast被引量：4收藏
- 作者：Yao, Chaoying Yin, Yu Li, Qingyang Zhang, Hanqi Zhang, Yilun Shao, Qianqian Liu, Qi Ren, Yanna Cai, Menghao
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2025
- 关键词：Gene expression regulation - Nucleotides - Transcription
- Rational design and characterization of enhanced alcohol-inducible synthetic promoters in Pichia pastoris被引量：3收藏
- 作者：Liu, Qi Li, Yun-hao Tao, Liu-fei Yang, Jia-yi Zhang, Yi-lun Cai, Meng-hao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2025
- 关键词：Pichia pastoris methanol ethanol synthetic promoter
- Heterologous Expression and Functional Characterization of a Truncated Marine Alginate Lyase被引量：1收藏
- 作者：Shao, Qianqian Yao, Chaoying Wang, Xingbin Han, Bei Liu, Qi Cai, Menghao
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2025
- 关键词：Alginate lyase
Komagataella phaffii Non-catalytic domain truncation Enzymatic property Heterologous expression
- From wheat straw to soluble yeast extract: enhanced mycoprotein production by adaptively evolved Trichosporon cutaneum and fermentation pH shifting被引量：0收藏
- 作者：Qu, Chaolong Ren, Dayu Liu, Qi Zhang, Bin Bao, Jie
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2025
- 关键词：Single cell protein Lignocellulose Adaptive evolution pH shifting Yeast extract
- Characterization and development of Salt-Resistant Aspergillus oryzae for Marine-derived Enzyme Expression被引量：0收藏
- 作者：Han, Bei Shao, Qianqian Zhang, Yilun Liu, Qi Cai, Menghao
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2025
- 关键词：Marine enzyme
A. oryzae Transcriptomic analysis Heterologous expression Alginate lyase
- Heavy chain variants affect light and heavy chains assembly of monoclonal antibody expressed by Pichia pastoris被引量：0收藏
- 作者：Gong, Xiulong Ye, Kaixiong Xu, Mingqiang Qian, Zhilan Liu, Qi Wang, Xiaolong Zhou, Xiangshan Liu, Haifeng Cai, Menghao
- 机构：East China Univ Sci & Technol;China Resources Biopharmaceut Co Ltd;China Resources Angde Biotech Pharma Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY 2025
- 关键词：Assembly monoclonal antibody
Pichia pastoris recombinant expression
- Programmable and controllable sexual life cycle for improved evolution in Komegataella phaffii被引量：0收藏
- 作者：Song, Lili Wu, Yanyan Hua, Li Liu, Qi Li, Yunhao Zhou, Xiangshan Cai, Menghao
- 机构：East China Univ Sci & Technol;China Resources Biopharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2026
- 关键词：Cell proliferation - Life cycle
- Adaptive Evolution of Rhodosporidium toruloides with Ultra-centrifugal Fractionation Leads to a High Microbial Lipid Production using Crude Glycerol Feedstock被引量：0收藏
- 作者：Wang, Xinru Liu, Qi Zhang, Bin Bao, Jie
- 机构：East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2025
- 关键词：Biodiesel Crude glycerol
Rhodosporidium toruloides Microbial lipid Ultra-centrifugal fractionation
- Synthetic yeast-bacterium consortium enables co-inducible relayed synthesis of chemicals被引量：0收藏
- 作者：Xu, Mingqiang Yu, Jiahui Chen, Xinjie Li, Xie Guo, Yan Qian, Zhilan Liu, Qi Ren, Yanna Lu, Jian Chen, Xianjun Yang, Yi Cai, Menghao
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2026
- 关键词：Synthetic consortiums Co-inducible Quorum sensing Optogenetics Komagataella phaffii Escherichia coli
- development and characterization of a Cre/loxP toolkit for genome engineering in Komagataella phaffii被引量：0收藏
- 作者：Song, Lili Hua, Li Wu, Yanyan Jiang, Xinyu Liu, Qi Zheng, Yitao Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2027
- 关键词：Komagataella phaffii Cre/loxP Genome engineering LoxP variant screening Orthogonal loxP variants
