华东理工大学机构知识库

成果/Result

Influence of High Solid Concentration on Enzymatic Hydrolysis and Fermentation of Steam-Exploded Corn Stover Biomass被引量：148收藏 分享

作者：Lu, Yifeng Wang, Yonghong Xu, Guoqian Chu, Ju Zhuang, Yingping Zhang, Siliang

机构：E China Univ Sci & Technol

来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY  2010

关键词：Bioethanol   Corn stover   High solid concentration   Enzymatic hydrolysis   Fermentation  

Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces被引量：143收藏 分享

作者：Wang, Weishan Li, Shanshan Li, Zilong Zhang, Jingyu Fan, Keqiang Tan, Gaoyi Ai, Guomin Lam, Sin Man Shui, Guanghou Yang, Zhiheng Lu, Hongzhong Jin, Pinjiao Li, Yihong Chen, Xiangyin Xia, Xuekui Liu, Xueting Dannelly, H. Kathleen Yang, Chen Yang, Yi Zhang, Siliang Alterovitz, Gil Xiang, Wensheng Zhang, Lixin

机构：Chinese Acad Sci;East China Univ Sci & Technol;Chinese Acad Agr Sci;Chinese Acad Sci;Qilu Univ Technol

来源：NATURE BIOTECHNOLOGY  2020

关键词：Ketones - Metabolism - Biochemistry - Bacteria - Metabolites - Glycerol  

巴斯德毕赤酵母的基因表达系统研究进展被引量：130收藏 分享

作者：欧阳立明 张惠展 张嗣良 刘志敏

机构：华东理工大学生物工程学院;军事医学科学院生物工程研究所

来源：《生物化学与生物物理进展》  2000

关键词：巴斯德毕赤酵母  基因表达系统  载体  受体  

摘要：巴斯德毕赤酵母是一种近年来广泛使用的基因表达系统 ,它具有表达率高、遗传稳定、产物可分泌、发酵工艺成熟等许多优点 .综述了该系统在载体类型、载体元件 (包括启动子、选择标记和信号肽序列 )、受体类型。

下载全文在线阅读

GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris被引量：113收藏 分享

作者：Qin, Xiulin Qian, Jiangchao Yao, Gaofeng Zhuang, Yingping Zhang, Siliang Chu, Ju

机构：E China Univ Sci & Technol

来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY  2011

Optimization of cellulase mixture for efficient hydrolysis of steam-exploded corn stover by statistically designed experiments被引量：112收藏 分享

作者：Zhou, Jin Wang, Yong-Hong Chu, Ju Luo, Ling-Zhi Zhuang, Ying-Ping Zhang, Si-Liang

机构：E China Univ Sci & Technol

来源：BIORESOURCE TECHNOLOGY  2009

关键词：Cellulase mixture   Optimization   Steam-exploded corn stover   Statistically designed experiments   Bioethanol  

Identification and purification of the main components of cellulases from a mutant strain of Trichoderma viride T 100-14被引量：94收藏 分享

作者：Zhou, Jin Wang, Yong-Hong Chu, Ju Zhuang, Ying-Ping Zhang, Si-Liang Yin, Peng

机构：E China Univ Sci & Technol

来源：BIORESOURCE TECHNOLOGY  2008

关键词：Trichoderma viride T 100-14   mutant strain   cellulase   identification   purification  

Reverse biological engineering of hrdB to enhance the production of avermectins in an industrial strain of Streptomyces avermitilis被引量：80收藏 分享

作者：Zhuo, Ying Zhang, Wenquan Chen, Difei Gao, Hong Tao, Jun Liu, Mei Gou, Zhongxuan Zhou, Xianlong Ye, Bang-Ce Zhang, Qing Zhang, Siliang Zhang, Li-Xin

机构：Chinese Acad Sci;E China Univ Sci & Technol;Hebei Veyong Biochem Co Ltd;Chinese Acad Sci

来源：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA  2010

关键词：precision engineering   RNA polymerase   overproduction  

Feature-matching Pattern-based Support Vector Machines for Robust Peptide Mass Fingerprinting被引量：79收藏 分享

作者：Li, Youyuan Hao, Pei Zhang, Siliang Li, Yixue

机构：E China Univ Sci & Technol;Shanghai Ctr Bioinformat Technol

来源：MOLECULAR & CELLULAR PROTEOMICS  2011

Multiple Propionyl Coenzyme A-Supplying Pathways for Production of the Bioplastic Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) in Haloferax mediterranei被引量：76收藏 分享

作者：Han, Jing Hou, Jing Zhang, Fan Ai, Guomin Li, Ming Cai, Shuangfeng Liu, Hailong Wang, Lei Wang, Zejian Zhang, Siliang Cai, Lei Zhao, Dahe Zhou, Jian Xiang, Hua

机构：Chinese Acad Sci;Univ Chinese Acad Sci;Chinese Acad Sci;E China Univ Sci & Technol

来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY  2013

Improved vitamin B₁₂ production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process被引量：74收藏 分享

作者：Wang, Ze-Jian Wang, Hui-Yuan Li, Yong-Liang Chu, Ju Huang, Ming-Zhi Zhuang, Ying-Ping Zhang, Si-Liang

机构：E China Univ Sci & Technol;Grp Hua Rong Pharmaceut Co Ltd

来源：BIORESOURCE TECHNOLOGY  2010

关键词：Vitamin B-12   Oxygen uptake rate   Pseudomonas denitrificans   Scale-up   Oxygen supply strategy  

发酵过程多水平问题及其生物反应器装置技术研究——基于过程参数相关的发酵过程优化与放大技术被引量：69收藏 分享

作者：张嗣良

机构：华东理工大学生物反应器国家重点实验室国家生化工程技术研究中心

来源：《中国工程科学》  2001

关键词：生物反应器  发酵  放大  过程优化  细胞培养  

摘要：回顾了发酵过程优化与放大所依据的基本思想和方法 ,认为采用以动力学为基础的最佳工艺控制点为依据的静态操作方法 ,实质上只是化学工程宏观动力学概念在发酵工程上的延伸 ,往往忽视细胞代谢流的存在。以细胞代谢流的分析与控制为核...

下载全文在线阅读

Development of a continuous cell-recycle fermentation system for production of lactic acid by Lactobacillus paracasei被引量：65收藏 分享

作者：Xu, Guo-qian Chu, Ju Wang, Yong-hong Zhuang, Ying-ping Zhang, Si-liang Peng, Hua-qiong

机构：E China Univ Sci & Technol

来源：PROCESS BIOCHEMISTRY  2006

关键词：electromagnetic flow-meter   Lactobacillus paracasei   lactic acid   tangential velocity   membrane bioreactor   cell-recycle  

Oxygen uptake rate optimization with nitrogen regulation for erythromycin production and scale-up from 50 L to 372 m³ scale被引量：64收藏 分享

作者：Zou, Xiang Hang, Hai-feng Chu, Ju Zhuang, Ying-ping Zhang, Si-liang

机构：E China Univ Sci & Technol;Southwest Univ

来源：BIORESOURCE TECHNOLOGY  2009

关键词：Erythromycin   Industrial scale   Metabolic flux   Oxygen uptake rate   Scale-up  

Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy - shaking and extra-light supplementation approach被引量：62收藏 分享

作者：Li, Xu Wang, Yong-Hong Zhang, Si-Liang Chu, Ju Zhang, Ming Huang, Ming-Zhi Zhuang, Ying-Ping

机构：E China Univ Sci & Technol

来源：INTERNATIONAL JOURNAL OF HYDROGEN ENERGY  2009

关键词：Photo-hydrogen production   Rhodobacter sphaeroides   Light intensity   Hydrogen production rate   Light attenuation kinetics  

Fluid dynamics investigation of variant impeller combinations by simulation and fermentation experiment被引量：61收藏 分享

作者：Xia, Jian-Ye Wang, Yong-Hong Zhang, Si-Liang Chen, Ning Yin, Peng Zhuang, Ying-Ping Chu, Ju

机构：E China Univ Chem Technol

来源：BIOCHEMICAL ENGINEERING JOURNAL  2009

关键词：Dissolved oxygen   Fluid mechanics   Gas-liquid mass transfer   Mixing   Mycelia fermentation   Shear environment  

Genetic modulation of the overexpression of tailoring genes eryK and eryG leading to the improvement of erythromycin a purity and production in Saccharopolyspora erythraea fermentation被引量：60收藏 分享

作者：Chen, Yun Deng, Wei Wu, Jiequn Qian, Jiangchao Chu, Ju Zhuang, Yingping Zhang, Siliang Liu, Wen

机构：E China Univ Sci & Technol;Chinese Acad Sci

来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY  2008

Flow Pattern, Mixing, Gas Hold-Up and Mass Transfer Coefficient of Triple-Impeller Configurations in Stirred Tank Bioreactors被引量：60收藏 分享

作者：Xie, Minghui Xia, Jianye Zhou, Zhen Chu, Ju Zhuang, Yingping Zhang, Siliang

机构：E China Univ Sci & Technol

来源：INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH  2014

关键词：Gases - Liquids - Mixing - Pumps - Turbine components - Flow patterns - Mass transfer - Turbomachine blades - Velocity  

Screening endogenous signal peptides and protein folding factors to promote the secretory expression of heterologous proteins in Pichia pastoris被引量：59收藏 分享

作者：Duan, Guangdong Ding, Lumei Wei, Dongsheng Zhou, Hangcheng Chu, Ju Zhang, Siliang Qian, Jiangchao

机构：East China Univ Sci & Technol

来源：JOURNAL OF BIOTECHNOLOGY  2019

关键词：Signal peptides   Folding factors   Pichia pastoris   Heterologous protein   Secretory expression  

Progress in the research of S-adenosyl-L-methionine production被引量：59收藏 分享

作者：Chu, Ju Qian, Jiangchao Zhuang, Yingping Zhang, Siliang Li, Yourong

机构：E China Univ Sci & Technol

来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY  2013

关键词：S-adenosyl-L-methionine   L-Methionine   ATP   L-Methionine adenosyltransferase   Pichia pastoris  

Industrial bioprocess control and optimization in the context of systems biotechnology被引量：56收藏 分享

作者：Wang, Yong Chu, Ju Zhuang, Yingping Wang, Yonghong Xia, Jianye Zhang, Siliang

机构： State Key Laboratory of Bioreactor Engineering

来源：Biotechnology Advances  2009

Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy - shaking and extra-light supplementation approach被引量：52收藏 分享

作者：Li, Xu Wang, Yong-Hong Zhang, Si-Liang Chu, Ju Zhang, Ming Huang, Ming-Zhi Zhuang, Ying-Ping

机构： State Key Laboratory of Bioreactor Engineering

来源：International Journal of Hydrogen Energy  2009

关键词：Image enhancement - Production efficiency  

重组巴氏毕赤酵母高密度发酵表达rHSA被引量：49收藏 分享

作者：郭美锦 庄英萍 储炬 张嗣良 刘志敏

机构：华东理工大学生物反应器工程国家重点实验室;军事医学科学院生物工程研究所

来源：《微生物学报》  2002

关键词：重组巴斯德毕赤酵母  高密度发酵  重组人血清白蛋白  rHSA  表达  

摘要：对基因工程菌Pichiapastoris的摇瓶发酵条件进行了试验 ,并根据摇瓶发酵的优化结果进行了补料分批高密度发酵。在摇瓶发酵时 ,甲醇诱导基因工程菌P .pastoris表达重组人血清白蛋白的发酵周期为 96h ;甲...

下载全文在线阅读

Application of Euler-Lagrange CFD for quantitative evaluating the effect of shear force on Carthamus tinctorius L. cell in a stirred tank bioreactor被引量：49收藏 分享

作者：Liu, Yu Wang, Ze-Jian Xia, Jian-Ye Haringa, Cees Liu, Ya-Ping Chu, Ju Zhuang, Ying-Ping Zhang, Si-Liang

机构：East China Univ Sci & Technol;Dalian Pract Biotechnol Co Ltd;Delft Univ Technol

来源：BIOCHEMICAL ENGINEERING JOURNAL  2016

关键词：Plant cell culture   Fluid mechanics   Agitation   Power consumption   C tinctorius L. cell   Stress thresholds  

Multiple propionyl coenzyme a-supplying pathways for production of the bioplastic poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) in Haloferax mediterranei被引量：49收藏 分享

作者：Han, Jing Hou, Jing Zhang, Fan Ai, Guomin Li, Ming Cai, Shuangfeng Liu, Hailong Wang, Lei Wang, Zejian Zhang, Siliang Cai, Lei Zhao, Dahe Zhou, Jian Xiang, Hua

机构： State Key Laboratory of Microbial Resources; University of Chinese Academy of Sciences; Beijing Institute of Genomics; State Key Laboratory of Bioreactor Engineering; University of Massachusetts

来源：Applied and Environmental Microbiology  2013

关键词：Biochemistry - Reinforced plastics - Coenzymes - Genes - Monomers  

CFD analysis of the turbulent flow in baffled shake flasks被引量：49收藏 分享

作者：Li, Chao Xia, Jian-Ye Chu, Ju Wang, Yong-Hong Zhuang, Ying-Ping Zhang, Si-Liang

机构：E China Univ Sci & Technol

来源：BIOCHEMICAL ENGINEERING JOURNAL  2013

关键词：Shake flask   Hydrodynamics   Mass transfer   Mixing   Shear force   Power consumption  

Power consumption, local and average volumetric mass transfer coefficient in multiple-impeller stirred bioreactors for xanthan gum solutions被引量：48收藏 分享

作者：Xie, Ming-hui Xia, Jian-ye Zhou, Zhen Zhou, Guo-zhong Chu, Ju Zhuang, Ying-ping Zhang, Si-liang Noorman, Henk

机构：E China Univ Sci & Technol;Wenzhou Great Wall Mixer Design Inst;DSM Biotechnol Ctr

来源：CHEMICAL ENGINEERING SCIENCE  2014

关键词：Power   Mass transfer   Xanthan gum   Non-Newtonian fluid   Impeller combination  

Effects of light/dark cycle, mixing pattern and partial pressure of H₂ on biohydrogen production by Rhodobacter sphaeroides ZX-5被引量：47收藏 分享

作者：Li, Xu Wang, Yonghong Zhang, Siliang Chu, Ju Zhang, Ming Huang, Mingzhi Zhuang, Yingping

机构：E China Univ Sci & Technol

来源：BIORESOURCE TECHNOLOGY  2011

关键词：Photo-hydrogen production   Rhodobacter sphaeroides   Light/dark cycle   Shaking velocity   Hydrogen partial pressure  

Understanding the effect of foreign gene dosage on the physiology of Pichia pastoris by transcriptional analysis of key genes被引量：47收藏 分享

作者：Zhu, Taicheng Guo, Meijin Zhuang, Yingping Chu, Ju Zhang, Siliang

机构：E China Univ Sci & Technol;Chinese Acad Sci

来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY  2011

关键词：Gene dosage   Pichia pastoris   Methanol metabolism   Cell physiology   mRNA quantitative analysis  

Improved large-scale production of vitamin B₁₂ by Pseudomonas denitrificans with betaine feeding被引量：45收藏 分享

作者：Li, Kun-Tai Liu, Dong-Hong Li, Yong-Liang Chu, Ju Wang, Yong-Hong Zhuang, Ying-Ping Zhang, Si-Liang

机构：E China Univ Sci & Technol;Jiangxi Agr Univ;Grp Hua Rong Pharmaceut Co Ltd

来源：BIORESOURCE TECHNOLOGY  2008

关键词：betaine   large-scale production   vitamin B-12   Pseudomonas denitrificans  

High efficient production of recombinant human consensus interferon mutant in high cell density culture of Pichia pastoris using two phases methanol control被引量：45收藏 分享

作者：Wu, Dan Chu, Ju Hao, Yu-You Wang, Yong-Hong Zhuang, Ying-Ping Zhang, Si-Liang

机构： State Key Laboratory of Bioreactor Engineering

来源：Process Biochemistry  2011

关键词：Aggregates - Physiology - Methanol - Batch cell culture - Covalent bonds - Growth rate - Quality control - Cell proliferation - Sulfur compounds - Yeast - Glycoproteins - Recombinant proteins