成果/Result
- Improved vitamin B12 production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process被引量：74收藏
- 作者：Wang, Ze-Jian Wang, Hui-Yuan Li, Yong-Liang Chu, Ju Huang, Ming-Zhi Zhuang, Ying-Ping Zhang, Si-Liang
- 机构：E China Univ Sci & Technol;Grp Hua Rong Pharmaceut Co Ltd
- 来源：BIORESOURCE TECHNOLOGY 2010
- 关键词：Vitamin B-12 Oxygen uptake rate Pseudomonas denitrificans Scale-up Oxygen supply strategy
- Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy - shaking and extra-light supplementation approach被引量：62收藏
- 作者：Li, Xu Wang, Yong-Hong Zhang, Si-Liang Chu, Ju Zhang, Ming Huang, Ming-Zhi Zhuang, Ying-Ping
- 机构：E China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 2009
- 关键词：Photo-hydrogen production Rhodobacter sphaeroides Light intensity Hydrogen production rate Light attenuation kinetics
- Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using a novel strategy - shaking and extra-light supplementation approach被引量：52收藏
- 作者：Li, Xu Wang, Yong-Hong Zhang, Si-Liang Chu, Ju Zhang, Ming Huang, Ming-Zhi Zhuang, Ying-Ping
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：International Journal of Hydrogen Energy 2009
- 关键词：Image enhancement - Production efficiency
- Effects of light/dark cycle, mixing pattern and partial pressure of H2 on biohydrogen production by Rhodobacter sphaeroides ZX-5被引量：47收藏
- 作者：Li, Xu Wang, Yonghong Zhang, Siliang Chu, Ju Zhang, Ming Huang, Mingzhi Zhuang, Yingping
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2011
- 关键词：Photo-hydrogen production Rhodobacter sphaeroides Light/dark cycle Shaking velocity Hydrogen partial pressure
- Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris被引量：45收藏
- 作者：Liu, Ping Huang, Mingzhi Guo, Menglei Qian, Jiangchao Lin, Weilu Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresources and Bioprocessing 2016
- 关键词：Gene expression - Physiology - Recombinant proteins - Yeast
- Machine Learning Algorithms for In-Line Monitoring During Yeast Fermentations Based on Raman Spectroscopy被引量：43收藏
- 作者：Wu, Debiao Xu, Yaying Xu, Feng Shao, Minghao Huang, Mingzhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：SSRN 2023
- 关键词：Ethanol - Feature Selection - Fermentation - Glucose - Glycerol - Learning algorithms - Process control - Production efficiency - Raman spectroscopy - Spectrum analysis - Voltage measurement - Yeast
- Improved vitamin B12 production by step-wise reduction of oxygen uptake rate under dissolved oxygen limiting level during fermentation process被引量：38收藏
- 作者：Wang, Ze-Jian Wang, Hui-Yuan Li, Yong-Liang Chu, Ju Huang, Ming-Zhi Zhuang, Ying-Ping Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering; Shijiazhuang Pharma
- 来源：Bioresource Technology 2010
- 关键词：Fermentation - Bacteria - Biosynthesis - Cell growth - Process control - Oxygen supply - Biochemistry - Growth kinetics
- Multi-omics integrative analysis with genome-scale metabolic model simulation reveals global cellular adaptation of Aspergillus niger under industrial enzyme production condition被引量：36收藏
- 作者：Lu, Hongzhong Cao, Weiqiang Liu, Xiaoyun Sui, Yufei Ouyang, Liming Xia, Jianye Huang, Mingzhi Zhuang, Yingping Zhang, Siliang Noorman, Henk Chu, Ju
- 机构：East China Univ Sci & Technol;DSM Biotechnol Ctr
- 来源：SCIENTIFIC REPORTS 2018
- Integrated isotope-assisted metabolomics and 13C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger被引量：35收藏
- 作者：Lu, Hongzhong Liu, Xiaoyun Huang, Mingzhi Xia, Jianye Chu, Ju Zhuang, Yingping Zhang, Siliang Noorman, Henk
- 机构：E China Univ Sci & Technol;DSM Biotechnol Ctr
- 来源：MICROBIAL CELL FACTORIES 2015
- 关键词：Aspergillus niger Glucoamylase C-13 metabolic flux analysis Metabolomics Cofactor metabolism
- Effects of light/dark cycle, mixing pattern and partial pressure of H2 on biohydrogen production by Rhodobacter sphaeroides ZX-5被引量：35收藏
- 作者：Li, Xu Wang, Yonghong Zhang, Siliang Chu, Ju Zhang, Ming Huang, Mingzhi Zhuang, Yingping
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2011
- 关键词：Fermentation - Substrates - Mixing - Partial pressure - Image enhancement
- 人工神经网络在红霉素发酵过程状态预估中的应用被引量：34收藏
- 作者：黄明志 杭海峰 储炬 叶勤 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2000
- 关键词：神经网络 BP网络 RBF网络 红霉素 发酵 状态预估
- 摘要：探索了动态 BP网络和 RBF网络在红霉素发酵过程状态预估中的应用 ,比较了它们的收敛速度和学习能力。结果表明 ,BP网络和 RBF网络都具有相当好的学习能力 ,但 RBF网络的收敛速度更快。训练好的神经网络 ,在红霉素...
- 下载全文在线阅读
- Impacts of high β-galactosidase expression on central metabolism of recombinant Pichia pastoris GS115 using glucose as sole carbon source via 13C metabolic flux analysis被引量：31收藏
- 作者：Nie, Yongsheng Huang, Mingzhi Lu, Junjie Qian, Jiangchao Lin, Weilu Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2014
- 关键词：P. pastoris C-13 metabolic flux analysis Central metabolism Heterologous protein expression
- Mixomics analysis of Bacillus subtilis: effect of oxygen availability on riboflavin production被引量：28收藏
- 作者：Hu, Junlang Lei, Pan Mohsin, Ali Liu, Xiaoyun Huang, Mingzhi Li, Liang Hu, Jianhua Hang, Haifeng Zhuang, Yingping Guo, Meijin
- 机构：East China Univ Sci & Technol;Shanghai Acebright Pharmaceut Grp Co Ltd
- 来源：MICROBIAL CELL FACTORIES 2017
- 关键词：Riboflavin Bacillus subtilis Oxygen availability C-13 metabolic flux analysis Metabolomics Transcriptomics
- Comprehensive reconstruction and in silico analysis of Aspergillus niger genome-scale metabolic network model that accounts for 1210 ORFs被引量：28收藏
- 作者：Lu, Hongzhong Cao, Weiqiang Ouyang, Liming Xia, Jianye Huang, Mingzhi Chu, Ju Zhuang, Yingping Zhang, Siliang Noorman, Henk
- 机构：East China Univ Sci & Technol;DSM Biotechnol Ctr
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2017
- 关键词：Aspergillus niger genome-scale metabolic model multi-omics glucoamylase
- A combined strategy to simultaneously improve the component of isovalerylspiramycin in a bitespiramycin (4-O-acylspiramycins) complex and its biological titre被引量：24收藏
- 作者：Wang, Yong-Hong Xi, Bing-xia Hao, Yu-You Huang, Ming-Zhi Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2008
- 关键词：Cost effectiveness - Biochemistry
- Controlling the feed rate of glucose and propanol for the enhancement of erythromycin production and exploration of propanol metabolism fate by quantitative metabolic flux analysis被引量：22收藏
- 作者：Chen, Yong Huang, Mingzhi Wang, Zejian Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol;Qingdao Agr Univ
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2013
- 关键词：Erythromycin Propanol Metabolic flux analysis Feeding strategy
- Metabolic flux analysis of the central carbon metabolism of the industrial vitamin B12 producing strain Pseudomonas denitrificans using 13C-labeled glucose被引量：20收藏
- 作者：Wang, Ze-Jian Wang, Ping Liu, Yu-Wei Zhang, Yi-Ming Chu, Ju Huang, Ming-zhi Zhuang, Ying-Ping Zhang, Si-Liang
- 机构： State Key Laboratory of Bioreactor Engineering; Microbiology Laboratory of Hebei Medical University; Department of Chemical and Biological Engineering
- 来源：Journal of the Taiwan Institute of Chemical Engineers 2012
- 关键词：Bacteria - Cultivation - Physiology - Carbon - Oxygen supply - Amino acids - Metabolism
- 柠檬酸钠对肌苷发酵过程代谢流分布的调节被引量：19收藏
- 作者：陈双喜 黄明志 储炬 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《化工学报》 2005
- 关键词：枯草杆菌 肌苷 碳源溢流 代谢流调节
- 摘要：基于物料的质量守恒定律和中间代谢物的拟稳态假设,定量研究了柠檬酸钠对肌苷发酵过程不同时段代谢流分布的影响.结果表明,添加的柠檬酸钠能够抑制存在于EMP途径和TCA循环之间的“碳源溢流”.在24h以后,EMP途径的代谢通量...
- 下载全文在线阅读
- 13C-assisted metabolomics analysis reveals the positive correlation between specific erythromycin production rate and intracellular propionyl-CoA pool size in Saccharopolyspora erythraea被引量：19收藏
- 作者：Hong, Ming Mou, Han Liu, Xiaoyun Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Acyl-CoAs Intracellular metabolites Isotope dilution mass spectroscopy Propionyl-CoA Saccharopolyspora erythraea
- Machine learning algorithms for in-line monitoring during yeast fermentations based on Raman spectroscopy被引量：18收藏
- 作者：Wu, Debiao Xu, Yaying Xu, Feng Shao, Minghao Huang, Mingzhi
- 机构：East China Univ Sci & Technol
- 来源：VIBRATIONAL SPECTROSCOPY 2024
- 关键词：Machine learning Raman spectroscopy Yeast fermentation Process analytical technology
- Engineering of succinyl-CoA metabolism in view of succinylation regulation to improve the erythromycin production被引量：17收藏
- 作者：Ke, Xiang Jiang, Xing Huang, Mingzhi Tian, Xiwei Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2022
- 关键词：Saccharopolyspora erythraea Succinylation Erythromycin Aconitase CRISPRi
- Exploring the metabolic fate of propanol in industrial erythromycin-producing strain via 13C labeling experiments and enhancement of erythromycin production by rational metabolic engineering of Saccharopolyspora erythraea被引量：16收藏
- 作者：Xu, Feng Ke, Xiang Hong, Ming Huang, Mingzhi Chen, Chongchong Tian, Xiwei Hang, Haifeng Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 2021
- 关键词：Saccharopolyspora erythraea Erythromycin Metabolic flux analysis N-Propanol Succinyl-CoA synthetase
- Effects of vitamins (nicotinic acid, vitamin B1 and biotin) on phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5被引量：16收藏
- 作者：Li, Xu Shi, Huan Wang, Yonghong Zhang, Siliang Chu, Ju Zhang, Ming Huang, Mingzhi Zhuang, Yingping
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：International Journal of Hydrogen Energy 2011
- 关键词：Image enhancement - Vitamins - Coenzymes - Batch cell culture
- 鸟苷发酵过程的定量和优化:抑制NH_4^+离子积累提高了苷产量70%被引量：14收藏
- 作者：黄明志 蔡显鹏 陈双喜 储炬 庄英萍 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室生化工程技术研究中心生物工程学院
- 来源：《生物工程学报》 2003
- 关键词：代谢流迁移 定量研究 鸟苷 枯草芽孢杆菌 代谢流分布
- 摘要：基于质量守恒定律和代谢反应中间代谢物的拟稳态假设 ,定量研究了枯草芽孢杆菌 (Bacillussubtilis)代谢流分布随时间变化的特征。研究结果表明 ,在鸟苷发酵过程 40h左右 ,存在着从己糖单磷酸途径到酵解途径的...
- 下载全文在线阅读
- Impacts of proline on the central metabolism of an industrial erythromycin-producing strain Saccharopolyspora erythraea via 13C labeling experiments被引量：13收藏
- 作者：Hong, Ming Huang, Mingzhi Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：C-13 metabolic flux analysis Proline Saccharopolyspora erythraea Chemically defined medium
- Effects of vitamins (nicotinic acid, vitamin B1 and biotin) on phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5被引量：13收藏
- 作者：Li, Xu Shi, Huan Wang, Yonghong Zhang, Siliang Chu, Ju Zhang, Ming Huang, Mingzhi Zhuang, Yingping
- 机构：E China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 2011
- 关键词：Photo-hydrogen production Rhodobacter sphaeroides Vitamin Nicotinic acid NAD(+)/NADH
- Blocking the flow of propionate into TCA cycle through a mutB knockout leads to a significant increase of erythromycin production by an industrial strain of Saccharopolyspora erythraea被引量：13收藏
- 作者：Chen, Chongchong Hong, Ming Chu, Ju Huang, Mingzhi Ouyang, Liming Tian, Xiwei Zhuang, Yingping
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Saccharopolyspora erythraea Erythromycin N-propanol Methylmalonyl-CoA mutase mutB
- Reconstruction of the Genome-Scale Metabolic Model of Saccharopolyspora erythraea and Its Application in the Overproduction of Erythromycin被引量：12收藏
- 作者：Xu, Feng Lu, Ju Ke, Xiang Shao, Minghao Huang, Mingzhi Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：METABOLITES 2022
- 关键词：genome-scale metabolic model Saccharopolyspora erythraea iJL1426 erythromycin production n-propanol process optimization
- Comprehensive reconstruction and evaluation of Pichia pastoris genome-scale metabolic model that accounts for 1243 ORFs被引量：12收藏
- 作者：Ye, Rui Huang, Mingzhi Lu, Hongzhong Qian, Jiangchao Lin, Weilu Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2017
- 关键词：Genome-scale metabolic model Pichia pastoris Multi-omics beta-Galactosidase
- Quantitative metabolic flux analysis revealed uneconomical utilization of ATP and NADPH in Acremonium chrysogenum fed with soybean oil被引量：12收藏
- 作者：Li, Jianhua Yang, Yiming Chu, Ju Huang, Mingzhi Li, Liang Zhang, Xiaochun Wang, Yonghong Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol;Shanxi Weiqida Pharmaceut Co Ltd
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2010
- 关键词：Metabolic flux analysis Acremonium chrysogenum Fed-batch cultivation Cephalosporin C
