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115 条 记 录,以下是 1-30

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The Important Role of Halogen Bond in Substrate Selectivity of Enzymatic Catalysis被引量:78收藏 分享
作者:Jiang, Shuiqin Zhang, Lujia Cui, Dongbin Yao, Zhiqiang Gao, Bei Lin, Jinping Wei, Dongzhi
机构:East China Univ Sci & Technol
来源:SCIENTIFIC REPORTS  2016
Enhanced production of dihydroxyacetone from glycerol by overexpression of glycerol dehydrogenase in an alcohol dehydrogenase-deficient mutant of Gluconobacter oxydans被引量:62收藏 分享
作者:Li, Ming-hua Wu, Jian Liu, Xu Lin, Jin-ping Wei, Dong-zhi Chen, Hao
机构:E China Univ Sci & Technol
来源:BIORESOURCE TECHNOLOGY  2010
关键词:Gluconobacter oxydans   Glycerol dehydrogenase   Dihydroxyacetone   Biotransformation   Glycerol  
Discovering multicore micelles: insights into the self-assembly of linear ABC terpolymers in midblock-selective solvents被引量:57收藏 分享
作者:Wang, Liquan Lin, Jiaping
机构:E China Univ Sci & Technol
来源:SOFT MATTER  2011
High cell density fermentation of Gluconobacter oxydans DSM 2003 for glycolic acid production被引量:48收藏 分享
作者:Wei, Guodong Yang, Xuepeng Gan, Tula Zhou, Wenyu Lin, Jinping Wei, Dongzhi
机构:E China Univ Sci & Technol;Zhengzhou Univ Light Ind
来源:JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY  2009
关键词:Gluconobacter oxydans   Uniform design   Fed-batch   High-density fermentation   Bioconversion   Ethylene glycol   Glycolic acid  
Optimization of immobilization for selective oxidation of benzyl alcohol by Gluconobacter oxydans using response surface methodology被引量:46收藏 分享
作者:Wu, Jian Wang, Jia-Le Li, Min-Hua Lin, Jin-Ping Wei, Dong-Zhi
机构:E China Univ Sci & Technol
来源:BIORESOURCE TECHNOLOGY  2010
关键词:Benzaldehyde production   Ca-alginate   Immobilization   Gluconobacter oxydans   Response surface methodology  
Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp for biodiesel production被引量:45收藏 分享
作者:Gao, Bei Su, Erzheng Lin, Jinping Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
机构:E China Univ Sci & Technol
来源:JOURNAL OF BIOTECHNOLOGY  2009
关键词:LipK107   E. coli whole-cell catalyst   Biodiesel   Low-temperature property  
Purification and Characterization of an Organic Solvent-Tolerant Lipase from Pseudomonas aeruginosa CS-2被引量:43收藏 分享
作者:Peng, Ren Lin, Jinping Wei, Dongzhi
机构:E China Univ Chem Technol;Jiangxi Normal Univ
来源:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY  2010
关键词:Organic solvent-tolerant lipase   Pseudomonas aeruginosa CS-2  
Overexpression of membrane-bound gluconate-2-dehydrogenase to enhance the production of 2-keto-D-gluconic acid by Gluconobacter oxydans被引量:39收藏 分享
作者:Li, Kefei Mao, Xinlei Liu, Liu Lin, Jinping Sun, Ming Wei, Dongzhi Yang, Shengli
机构:East China Univ Sci & Technol
来源:MICROBIAL CELL FACTORIES  2016
关键词:2-keto-D-gluconic acid   Gluconobacter oxydans   Overexpression   Resting cell  
Pyrroloquinoline quinone biosynthesis in Escherichia coli through expression of the Gluconobacter oxydans pqqABCDE gene cluster被引量:38收藏 分享
作者:Yang, Xue-Peng Zhong, Gui-Fang Lin, Jin-Ping Mao, Duo-Bin Wei, Dong-Zhi
机构:Zhengzhou Univ Light Ind;E China Univ Sci & Technol
来源:JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY  2010
关键词:Biosynthesis   Expression   Gene cluster   Pyrroloquinoline quinone  
Enhanced production of dihydroxyacetone from glycerol by overexpression of glycerol dehydrogenase in an alcohol dehydrogenase-deficient mutant of Gluconobacter oxydans被引量:37收藏 分享
作者:Li, Ming-hua Wu, Jian Liu, Xu Lin, Jin-ping Wei, Dong-zhi Chen, Hao
机构: State Key Laboratory of Bioreactor Engineering
来源:Bioresource Technology  2010
关键词:Bioconversion - Polymerase chain reaction - Productivity - Enzyme activity - Genes  
Improving the catalytic activity of lipase LipK107 from Proteus sp. by site-directed mutagenesis in the lid domain based on computer simulation被引量:34收藏 分享
作者:Gao, Bei Xu, Tao Lin, Jinping Zhang, Lujia Su, Erzheng Jiang, Zhengbing Wei, Dongzhi
机构: State Key Laboratory of Bioreactor Engineering; College of Life Science
来源:Journal of Molecular Catalysis B: Enzymatic  2011
关键词:Lipases - Palmitic acid - Mutagenesis - Catalysis  
Development of recombinant Escherichia coli whole-cell biocatalyst expressing a novel alkaline lipase-coding gene from Proteus sp. for biodiesel production被引量:33收藏 分享
作者:Gao, Bei Su, Erzheng Lin, Jinping Jiang, Zhengbing Ma, Yushu Wei, Dongzhi
机构: State Key Laboratory of Bioreactor Engineering
来源:Journal of Biotechnology  2009
关键词:Catalysts - Cloning - Cytology - Gene encoding - Temperature - Biodiesel - Purification - Alkalinity  
Optimization of immobilization for selective oxidation of benzyl alcohol by Gluconobacter oxydans using response surface methodology被引量:32收藏 分享
作者:Wu, Jian Wang, Jia-Le Li, Min-Hua Lin, Jin-Ping Wei, Dong-Zhi
机构: New World Institute of Biotechnology
来源:Bioresource Technology  2010
关键词:Alginate - Cell immobilization - Surface properties  
Functions of membrane-bound alcohol dehydrogenase and aldehyde dehydrogenase in the bio-oxidation of alcohols in Gluconobacter oxydans DSM 2003被引量:31收藏 分享
作者:Wei, Liu-Jing Zhou, Ji-Lai Zhu, Dan-Ni Cai, Bai-Yi Lin, Jin-Ping Hua, Qiang Wei, Dong-Zhi
机构: State Key Laboratory of Bioreactor Engineering; State Key Laboratory of Bioreactor Engineering
来源:Biotechnology and Bioprocess Engineering  2012
关键词:Enzymes - Oxidation - Aldehydes  
Towards the computational design and engineering of enzyme enantioselectivity: A case study by a carbonyl reductase from Gluconobacter oxydans被引量:31收藏 分享
作者:Deng, Jian Yao, Zhiqiang Chen, Kangling Yuan, Y. Adam Lin, Jinping Wei, Dongzhi
机构:E China Univ Sci & Technol;Natl Univ Singapore
来源:JOURNAL OF BIOTECHNOLOGY  2016
关键词:Carbonyl reductase   Enantiorecognition   Computational design   OPBE   Improved enantioselectivity  
Membrane-bound pyrroloquinoline quinone-dependent dehydrogenase in Gluconobacter oxydans M5, responsible for production of 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose被引量:29收藏 分享
作者:Yang, Xue-Peng Wei, Liu-Jing Lin, Jin-Ping Yin, Bo Wei, Dong-Zhi
机构:E China Univ Sci & Technol;Zhengzhou Univ Light Ind
来源:APPLIED AND ENVIRONMENTAL MICROBIOLOGY  2008
Adsorptive bioconversion of ethylene glycol to glycolic acid by Gluconobacter oxydans DSM 2003被引量:28收藏 分享
作者:Wei, Guodong Yang, Xuepeng Zhou, Wenyu Lin, Jinping Wei, Dongzhi
机构:E China Univ Sci & Technol;Zhengzhou Univ Light Ind
来源:BIOCHEMICAL ENGINEERING JOURNAL  2009
关键词:Gluconobacter oxydans   Glycolic acid   Ethylene glycol   Product inhibition   Integrated bioprocess   In situ product removal  
Drug-carrier/hydrogel scaffold for controlled growth of cells被引量:27收藏 分享
作者:Wei, Lan Lin, Jiaping Cai, Chunhua Fang, Zhengdong Fu, Weiguo
机构:E China Univ Sci & Technol;Fudan Univ
来源:EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS  2011
关键词:Scaffold   Drug delivery   Hydrogel   Micelle   Alginate  
Functional expression of a novel alkaline-adapted lipase of Bacillus amyloliquefaciens from stinky tofu brine and development of immobilized enzyme for biodiesel production被引量:26收藏 分享
作者:Cai, Xianghai Ma, Jing Wei, Dong-zhi Lin, Jin-ping Wei, Wei
机构:E China Univ Sci & Technol
来源:ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY  2014
关键词:Bacillus amyloliquefaciens   Lipase   Gene cloning   Functional expression   Immobilization  
Characterization of a Putative Stereoselective Oxidoreductase from Gluconobacter oxydans and Its Application in Producing Ethyl (R)-4-Chloro-3-Hydroxybutanoate Ester被引量:26收藏 分享
作者:Liu, Xu Chen, Rong Yang, Zhongwei Wang, Jiale Lin, Jinping Wei, Dongzhi
机构:E China Univ Sci & Technol;Hangzhou Normal Univ
来源:MOLECULAR BIOTECHNOLOGY  2014
关键词:Gluconobacter oxydans   Oxidoreductase   Ethyl (R)-4-chloro-3-hydroxybutanoate ester  
Carbonyl reductase identification and development of whole-cell biotransformation for highly efficient synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol被引量:25收藏 分享
作者:Chen, Kangling Li, Kefei Deng, Jian Zhang, Baoqi Lin, Jinping Wei, Dongzhi
机构:East China Univ Sci & Technol
来源:MICROBIAL CELL FACTORIES  2016
关键词:(R)-[3,5-bis(trifluoromethyl)phenyl] ethanol   3,5-bis(trifluoromethyl) acetophenone   Fusion-protein expression   Carbonyl reductase   Glucose dehydrogenase  
A novel strain Pseudomonas aeruginosa CS-2 producing an organic solvent-tolerant lipase被引量:25收藏 分享
作者:Peng, Ren Lin, Jinping Wei, Dongzhi
机构: State Key Laboratory of Bioreactor Engineering; College of Life Science
来源:Advanced Materials Research  2012
关键词:Bacteria - Lipases - Hexane - Magnesium compounds - Organic solvents - Chlorine compounds  
Identification of a novel promoter gHp0169 for gene expression in Gluconobacter oxydans被引量:25收藏 分享
作者:Shi, Lulu Li, Kefei Zhang, Huan Liu, Xu Lin, Jinping Wei, Dongzhi
机构:E China Univ Sci & Technol
来源:JOURNAL OF BIOTECHNOLOGY  2014
关键词:Gluconobacter oxydans   Promoter   gHp0169   Gene overexpression  
Development of a two-step process for production of 3-hydroxypropionic acid from glycerol using Klebsiella pneumoniae and Gluconobacter oxydans被引量:24收藏 分享
作者:Zhao, Li Lin, Jinping Wang, Hualei Xie, Jingli Wei, Dongzhi
机构:E China Univ Sci & Technol
来源:BIOPROCESS AND BIOSYSTEMS ENGINEERING  2015
关键词:3-Hydroxypropionic acid   Glycerol   Klebsiella pneumoniae   Gluconobacter oxydans   Two-step process  
Application of comparative proteome analysis to reveal influence of cultivation conditions on asymmetric bioreduction of β-keto ester by Saccharomyces cerevisiae被引量:24收藏 分享
作者:Lin, Jinping Liu, Qinghai Su, Erzheng Wei, Dongzhi Yang, Shengli
机构: State Key Laboratory of Bioreactor Engineering
来源:Applied Microbiology and Biotechnology  2008
关键词:Batch cell culture - Cultivation - Esters - Metabolism - Cells - Enzymes  
具有高谷胱甘肽合成活性重组大肠杆菌的构建及合成反应过程被引量:24收藏 分享
作者:李华钟 李寅 林金萍 陈坚
机构:无锡轻工大学生物工程学院;华东理工大学生物工程学院
来源:《微生物学报》  2001
关键词:谷胱甘肽  重组大肠杆菌  构建  生物合成  反应机理  
摘要:以野生型大肠杆菌E .coliⅡ为宿主细胞 ,转化带有编码谷胱甘肽合成酶系的基因gshⅠ和 gshⅡ的质粒 pGH50 1 ,获得了一株谷胱甘肽合成活性、质粒稳定性和传代稳定性俱佳 ,并且能够重复使用的重组大肠杆菌E ....
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Design and engineering of whole-cell biocatalyst for efficient synthesis of (R)-citronellal被引量:24收藏 分享
作者:Zhang, Baoqi Du, Han Zheng, Yanqiu Sun, Jiale Shen, Yu Lin, Jinping Wei, Dongzhi
机构:East China Univ Sci & Technol
来源:MICROBIAL BIOTECHNOLOGY  2022
A computational strategy for altering an enzyme in its cofactor preference to NAD(H) and/or NADP(H)被引量:23收藏 分享
作者:Cui, Dongbing Zhang, Lujia Jiang, Shuiqin Yao, Zhiqiang Gao, Bei Lin, Jinping Yuan, Y. Adam Wei, Dongzhi
机构:E China Univ Sci & Technol;Natl Univ Singapore
来源:FEBS JOURNAL  2015
关键词:altered coenzyme specificity   molecular dynamics simulation   rational computational design   site-directed mutagenesis   structure stability prediction  
Enhancement of cell growth and glycolic acid production by overexpression of membrane-bound alcohol dehydrogenase in Gluconobacter oxydans DSM 2003被引量:23收藏 分享
作者:Zhang, Huan Shi, Lulu Mao, Xinlei Lin, Jinping Wei, Dongzhi
机构:East China Univ Sci & Technol
来源:JOURNAL OF BIOTECHNOLOGY  2016
关键词:Gluconobacter oxydans   Alcohol dehydrogenase   Respiratory chain   Glycolic acid  
Identification of a yeast old yellow enzyme for highly enantioselective reduction of citral isomers to (R)-citronellal被引量:22收藏 分享
作者:Meng, Liandan Lin, Jinping Mang, Baoqi Kuang, Yuyao Wei, Dongzhi
机构:East China Univ Sci & Technol;East China Univ Sci & Technol
来源:BIORESOURCES AND BIOPROCESSING  2018
关键词:Enoate reductase   Old yellow enzyme (OYE)   Citral   (R)-Citronellal   Enantioselectivity   Asymmetric reduction  
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