成果/Result
- Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol被引量：123收藏
- 作者：Liu, Yiqi Tu, Xiaohu Xu, Qin Bai, Chenxiao Kong, Chuixing Liu, Qi Yu, Jiahui Peng, Qiangqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Methanol Methylotrophic yeast Co-culture Heterologous biosynthesis Monacolin J Lovastatin
- Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris被引量：95收藏
- 作者：Wang, Xiaolong Wang, Qi Wang, Jinjia Bai, Peng Shi, Lei Shen, Wei Zhou, Mian Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：E China Univ Sci & Technol;SCICB
- 来源：JOURNAL OF BIOLOGICAL CHEMISTRY 2016
- 关键词：DNA-protEIn interaction protEIn domain signal transduction transcription coactivator transcription regulation AOX1 promoter Mit1 Pichia pastoris glycerol repression methanol induction
- Upconversion fluorescent strip sensor for rapid determination of Vibrio anguillarum被引量：85收藏
- 作者：Zhao, Peng Wu, Yuanyuan Zhu, Yihua Yang, Xiaoling Jiang, Xin Xiao, Jingfan Zhang, Yuanxing Li, Chunzhong
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：NANOSCALE 2014
- 关键词：Assays - Monoclonal antibodies
- N-Fused quinoxalines and benzoquinoxalines as attractive emitters for organic light emitting diodes被引量：74收藏
- 作者：Lindner, Benjamin D. Zhang, Yexiang Hoefle, Stefan Berger, Nancy Teusch, Claudia Jesper, Malte Hardcastle, Kenneth I. Qian, Xuhong Lemmer, Uli Colsmann, Alexander Bunz, Uwe H. F. Hamburger, Manuel
- 机构：Heidelberg Univ;E China Univ Sci & Technol;KIT;Georgia Inst Technol;InnovationLab GmbH
- 来源：JOURNAL OF MATERIALS CHEMISTRY C 2013
- 关键词：Quantum theory - Organic light emitting diodes (OLED)
- Soluble Diazaiptycenes: Materials for Solution-Processed Organic Electronics被引量：64收藏
- 作者：Biegger, Philipp Stolz, Sebastian Intorp, Sebastian N. Zhang, Yexiang Engelhart, Jens U. Rominger, Frank Hardcastle, Kenneth I. Lemmer, Uli Qian, Xuhong Hamburger, Manuel Bunz, U. H. F.
- 机构：Heidelberg Univ;InnovationLab GmbH;Karlsruher Inst Technol;Karlsruhe Inst Technol;Georgia Inst Technol
- 来源：JOURNAL OF ORGANIC CHEMISTRY 2015
- 关键词：Quantum chemistry - Crystallization - Organic light emitting diodes (OLED)
- Biospecific Self-Assembly of a Nanoparticle Coating for Targeted and Stimuli-Responsive Drug Delivery被引量：54收藏
- 作者：Li, Jinyang Qu, Xue Payne, Gregory F. Zhang, Cheng Zhang, Yuxin Li, Jianbo Ren, Jie Hong, Hua Liu, Changsheng
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;Inst Biosyst & Biotechnol Res;Fischell Dept Bioengn;Tongji Univ
- 来源：ADVANCED FUNCTIONAL MATERIALS 2015
- 关键词：Cells - GlycoprotEIns - Cancer cells - Cell culture - Diseases - ProtEIns - Medical nanotechnology - Self assembly - Silica nanoparticles
- Nitrogen metabolism of asparagine and glutamate in Vero cells studied by 1H/15N NMR spectroscopy被引量：52收藏
- 作者：Huang, Haiyan Yu, Yihua Yi, Xiaoping Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai Key Laboratory of Functional Magnetic Resonance Imaging
- 来源：Applied Microbiology and Biotechnology 2007
- 关键词：Ammonia - Metabolism - Metabolites - Nitrogen - Nuclear magnetic resonance spectroscopy
- Involvement of LuxS in the regulation of motility and flagella biogenesis in Vibrio alginolyticus被引量：51收藏
- 作者：Tian, Yang Wang, Qiyao Liu, Qin Ma, Yue Cao, Xiaodan Guan, Lingyu Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioscience, Biotechnology and Biochemistry 2008
- 关键词：Bacteria - Bioassay - Gene expression - Real time control
- Catabolite Repression of Aox in Pichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy被引量：50收藏
- 作者：Zhang, Ping Zhang, Wenwen Zhou, Xiangshan Bai, Peng Cregg, James M. Zhang, Yuanxing
- 机构： Keck Graduate Institute of Applied Life Sciences; State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2010
- 关键词：Glucose sensors - Glucose oxidase - Fructose - Gene expression - Yeast
- An invasive and low virulent Edwardsiella tarda esrB mutant promising as live attenuated vaccine in aquaculture被引量：49收藏
- 作者：Yang, Weizheng Wang, Lixia Zhang, Lingzhi Qu, Jiangbo Wang, Qiyao Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;TianYuan Aquat Prod Co Ltd
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2015
- 关键词：Edwardsiella tarda T3SS T6SS esrB Invasive phenotype Vaccine
- Roles of Hfq in the stress adaptation and virulence in fish pathogen Vibrio alginolyticus and its potential application as a target for live attenuated vaccine被引量：49收藏
- 作者：Liu, Huan Wang, Qiyao Liu, Qin Cao, Xiaodan Shi, Cunbin Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Chinese Acad Fishery Sci
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：Hfq Quorum sensing Stress resistance Extracellular protease Vibrio alginolyticus
- Alkynylated Phenazines: Synthesis, Characterization, and Metal-Binding Properties of ThEIr Bis-Triazolyl Cycloadducts被引量：48收藏
- 作者：Bryant, Jonathan J. Zhang, Yexiang Lindner, Benjamin D. Davey, Evan A. Appleton, Anthony Lucas Qian, Xuhong Bunz, U. H. F.
- 机构：Heidelberg Univ;Georgia Inst Technol;E China Univ Sci & Technol
- 来源：JOURNAL OF ORGANIC CHEMISTRY 2012
- 关键词：Binding energy - Metals - Energy gap - Emission spectroscopy - Ethylene - Solutions - Metal ions
- Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast被引量：45收藏
- 作者：Liu, Yiqi Bai, Chenxiao Liu, Qi Xu, Qin Qian, Zhilan Peng, Qiangqiang Yu, Jiahui Xu, Mingqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Ethanol Crabtree-negative yeast Acetyl-CoA Monacolin J Metabolic engineering Coculture
- 阿胶酶解成分对贫血小鼠造血系统的保护机制被引量：41收藏
- 作者：吴宏忠 杨帆 崔书亚 秦玉峰 张元兴 刘建文
- 机构：华东理工大学生物反应器工程国家重点实验室;山东东阿阿胶集团;华东理工大学药学院
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：阿胶 活性成分制备 补血机理
- 摘要：运用5-氟脲嘧啶制备的小鼠贫血模型研究降解分离后所得阿胶有效组分A、B的升高红、白血球的作用机制。结果显示:体外消化液中提取的A、B组分能促进贫血小鼠外周血白细胞和红细胞的升高,促进骨髓和脾造血干/祖细胞集落BFU-E,...
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- Characterization of Edwardsiella tarda rpoS: Effect on serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression被引量：40收藏
- 作者：Xiao, Jingfan Wang, Qiyao Liu, Qin Xu, Lili Wang, Xin Wu, Haizhen Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2009
- 关键词：Biofilms - Body fluids - Gene expression
- Insight into metabolism of CHO cells at low glucose concentration on the basis of the determination of intracellular metabolites被引量：39收藏
- 作者：Lu, Subiao Sun, Xiangming Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2005
- 关键词：CHO cells low glucose intracellular amino acids
- Regulating unfolded protEIn response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris被引量：39收藏
- 作者：Huang, Mengmeng Gao, Yanyun Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Unfolded protEIn response HAC1p Heterologous expression P. pastoris Raw-starch hydrolyzing alpha-amylase
- PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris被引量：38收藏
- 作者：Wang, Xiaolong Cai, Menghao Shi, Lei Wang, Qi Zhu, Jinxiang Wang, Jinjia Zhou, Mian Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY LETTERS 2016
- 关键词：AOX1 promoter Catabolite repression Glucose Glycerol PpNrg1 Pichia pastoris Transcriptional regulation
- Interplay between ferric uptake regulator Fur and horizontally acquired virulence regulator EsrB coordinates virulence gene expression in Edwardsiella piscicida被引量：38收藏
- 作者：Shao, Shuai Li, Chunli Zhao, Luyao Zhang, Yuanxing Yin, Kaiyu Wang, Qiyao
- 机构：East China Univ Sci & Technol;Shanghai Business Sch;Qingdao Natl Lab Marine Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai;Shanghai Engn Res Ctr Maricultured Anim Vaccines
- 来源：MICROBIOLOGICAL RESEARCH 2021
- 关键词：Edwardsiella piscicida iron uptake system Fur EsrB biofilm formation virulence
- Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation被引量：37收藏
- 作者：Xiao, Anfeng Zhou, Xiangshan Zhou, Li Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2006
- 关键词：Biotechnology - Cell membranes - Cells - Fermentation - Microbiology - Oxygen
- Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway被引量：36收藏
- 作者：Xue, Ying Kong, Chuixing Shen, Wei Bai, Chenxiao Ren, Yanna Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Polyketides Pichia pastoris Heterologous biosynthesis Citrinin Biosynthetic pathway
- Isolation of a Virulent Aeromonas salmonicida subsp. masoucida Bacteriophage and Its Application in Phage Therapy in Turbot (Scophthalmus maximus)被引量：36收藏
- 作者：Xu, Zhenhe Jin, Peng Zhou, Xiangshan Zhang, Yuanxing Wang, Qiyao Liu, Xiaohong Shao, Shuai Liu, Qin
- 机构：East China Univ Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai;Shanghai Engn Res Ctr Maricultured Anim Vaccines
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2021
- 关键词：Aeromonas salmonicida subsp. masoucida antibacterial aquaculture phage therapy turbot Scophthalmus maximus
- Improving intracellular production of recombinant protEIn in Pichia pastoris using an optimized prEInduction glycerol-feeding scheme被引量：36收藏
- 作者：Wei, Chun Zhou, Xiangshan Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2008
- 关键词：Cells - Fermentation - Glycerol - Growth rate - Hormones - ProtEIns
- Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously被引量：35收藏
- 作者：Sun, Xueqian Zhou, Xiangshan Cai, Menghao Tao, Kejing Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2009
- 关键词：Ketones - Biosynthesis - Metabolites
- A programmable high-expression yeast platform responsive to user-defined signals被引量：35收藏
- 作者：Liu, Qi Song, Lili Peng, Qiangqiang Zhu, Qiaoyun Shi, Xiaona Xu, Mingqiang Wang, Qiyao Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SCIENCE ADVANCES 2022
- 关键词：Recombinant protEIns - Transcription
- Fed-batch culture of hybridoma cells in serum-free medium using an optimized feeding strategy被引量：35收藏
- 作者：Zhang, Li Shen, Hong Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 2004
- 关键词：hybridoma cell serum-free medium fed-batch culture kinetic model
- Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungus Aspergillus glaucus被引量：34收藏
- 作者：Sun, Xueqian Zhou, Xiangshan Cai, Menghao Zhou, Jiushun Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2010
- 关键词：Carboxylic acids - Biosynthesis - Ketones - Biochemistry
- Significance of seed culture methods on mycelial morphology and production of a novel anti-cancer anthraquinone by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403)被引量：34收藏
- 作者：Yu, Chao Cai, Menghao Kang, Li Zhang, Yuanxing Zhou, Xiangshan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2012
- 关键词：Mycelium - Aromatic compounds - Biomass - Bottles - Diseases - Ketones
- Metabolic characteristics of recombinant Chinese hamster ovary cells expressing glutamine synthetase in presence and absence of glutamine被引量：32收藏
- 作者：Zhang, Fang Sun, Xiangming Yi, Xiaoping Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：CYTOTECHNOLOGY 2006
- 关键词：animal cell culture cell metabolism glutamine glutamine synthetase recombinant CHO cell
- A novel multivalent vaccine based on secretary antigen-delivery induces protective immunity against Vibrio anguillarum and Aeromonas hydrophila被引量：31收藏
- 作者：Zhou, Lingyun Wang, Xiuhua Liu, Qin Wang, Qiyao Zhao, Yan Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering; Yellow Sea Fisheries Research Institute
- 来源：Journal of Biotechnology 2010
- 关键词：Physiology - Strain - Antigens
