成果/Result
- Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol被引量：123收藏
- 作者：Liu, Yiqi Tu, Xiaohu Xu, Qin Bai, Chenxiao Kong, Chuixing Liu, Qi Yu, Jiahui Peng, Qiangqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Methanol Methylotrophic yeast Co-culture Heterologous biosynthesis Monacolin J Lovastatin
- Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris被引量：95收藏
- 作者：Wang, Xiaolong Wang, Qi Wang, Jinjia Bai, Peng Shi, Lei Shen, Wei Zhou, Mian Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：E China Univ Sci & Technol;SCICB
- 来源：JOURNAL OF BIOLOGICAL CHEMISTRY 2016
- 关键词：DNA-protEIn interaction protEIn domain signal transduction transcription coactivator transcription regulation AOX1 promoter Mit1 Pichia pastoris glycerol repression methanol induction
- Catabolite Repression of Aox in Pichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy被引量：50收藏
- 作者：Zhang, Ping Zhang, Wenwen Zhou, Xiangshan Bai, Peng Cregg, James M. Zhang, Yuanxing
- 机构： Keck Graduate Institute of Applied Life Sciences; State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2010
- 关键词：Glucose sensors - Glucose oxidase - Fructose - Gene expression - Yeast
- Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast被引量：45收藏
- 作者：Liu, Yiqi Bai, Chenxiao Liu, Qi Xu, Qin Qian, Zhilan Peng, Qiangqiang Yu, Jiahui Xu, Mingqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Ethanol Crabtree-negative yeast Acetyl-CoA Monacolin J Metabolic engineering Coculture
- Regulating unfolded protEIn response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris被引量：39收藏
- 作者：Huang, Mengmeng Gao, Yanyun Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Unfolded protEIn response HAC1p Heterologous expression P. pastoris Raw-starch hydrolyzing alpha-amylase
- PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris被引量：38收藏
- 作者：Wang, Xiaolong Cai, Menghao Shi, Lei Wang, Qi Zhu, Jinxiang Wang, Jinjia Zhou, Mian Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY LETTERS 2016
- 关键词：AOX1 promoter Catabolite repression Glucose Glycerol PpNrg1 Pichia pastoris Transcriptional regulation
- Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation被引量：37收藏
- 作者：Xiao, Anfeng Zhou, Xiangshan Zhou, Li Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2006
- 关键词：Biotechnology - Cell membranes - Cells - Fermentation - Microbiology - Oxygen
- Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway被引量：36收藏
- 作者：Xue, Ying Kong, Chuixing Shen, Wei Bai, Chenxiao Ren, Yanna Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Polyketides Pichia pastoris Heterologous biosynthesis Citrinin Biosynthetic pathway
- Isolation of a Virulent Aeromonas salmonicida subsp. masoucida Bacteriophage and Its Application in Phage Therapy in Turbot (Scophthalmus maximus)被引量：36收藏
- 作者：Xu, Zhenhe Jin, Peng Zhou, Xiangshan Zhang, Yuanxing Wang, Qiyao Liu, Xiaohong Shao, Shuai Liu, Qin
- 机构：East China Univ Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai;Shanghai Engn Res Ctr Maricultured Anim Vaccines
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2021
- 关键词：Aeromonas salmonicida subsp. masoucida antibacterial aquaculture phage therapy turbot Scophthalmus maximus
- Improving intracellular production of recombinant protEIn in Pichia pastoris using an optimized prEInduction glycerol-feeding scheme被引量：36收藏
- 作者：Wei, Chun Zhou, Xiangshan Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2008
- 关键词：Cells - Fermentation - Glycerol - Growth rate - Hormones - ProtEIns
- Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously被引量：35收藏
- 作者：Sun, Xueqian Zhou, Xiangshan Cai, Menghao Tao, Kejing Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2009
- 关键词：Ketones - Biosynthesis - Metabolites
- Significance of seed culture methods on mycelial morphology and production of a novel anti-cancer anthraquinone by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403)被引量：34收藏
- 作者：Yu, Chao Cai, Menghao Kang, Li Zhang, Yuanxing Zhou, Xiangshan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2012
- 关键词：Mycelium - Aromatic compounds - Biomass - Bottles - Diseases - Ketones
- Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungus Aspergillus glaucus被引量：34收藏
- 作者：Sun, Xueqian Zhou, Xiangshan Cai, Menghao Zhou, Jiushun Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2010
- 关键词：Carboxylic acids - Biosynthesis - Ketones - Biochemistry
- Roles of cobalt in biosynthesis stimulation of a cytotoxic compound from marine-derived Aspergillus glaucus被引量：30收藏
- 作者：Cai, Menghao Sun, Xueqian Zhou, Xiangshan Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2012
- 关键词：Aspergillus - Biochemistry - Biosynthesis - Cobalt - Ketones - Trace elements
- Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism被引量：30收藏
- 作者：Xu, Qin Bai, Chenxiao Liu, Yiqi Song, Lili Tian, Lin Yan, Yunfeng Zhou, Jinfeng Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY FOR BIOFUELS 2019
- 关键词：Komagataella phaffii Acetate utilization Acetyl-CoA Kinase screening Metabolic engineering
- Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus被引量：30收藏
- 作者：Cai, Meng-Hao Zhou, Xiang-Shan Sun, Xue-Qian Tao, Ke-Jing Zhang, Yuan-Xing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Industrial Microbiology and Biotechnology 2009
- 关键词：Carbon - Nitrogen - Sodium - Statistical methods - Yeast
- Polyketides in Aspergillus terreus: biosynthesis pathway discovery and application被引量：29收藏
- 作者：Yin, Ying Cai, Menghao Zhou, Xiangshan Li, Zhiyong Zhang, Yuanxing
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016
- 关键词：Polyketide Aspergillus terreus Biosynthesis pathway Gene cluster Heterologous expression
- Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus被引量：28收藏
- 作者：Cai, Menghao Zhou, Xiangshan Zhou, Jiushun Niu, Chuanpeng Kang, Li Sun, Xueqian Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2010
- 关键词：Biochemistry - Sodium compounds - pH effects - Citric acid
- Highly sialylated recombinant human erythropoietin production in large-scale perfusion bioreactor utilizing CHO-gmt4 (JW152) with restored GnT I function被引量：21收藏
- 作者：Goh, John S. Y. Liu, Yingwei Liu, Haifeng Chan, Kah Fai Wan, Corrine Teo, Gavin Zhou, Xiangshan Xie, Fusheng Zhang, Peiqing Zhang, Yuanxing Song, Zhiwei
- 机构：ASTAR;E China Univ Sci & Technol;Shandong Dong E E Jiao Co Lte;Shandong Ehua Biopharmaceut Co Ltd
- 来源：BIOTECHNOLOGY JOURNAL 2014
- 关键词：CHO cells Erythropoietin (EPO) Glycosylation N-Acetylglucosaminyltransferase-I (GnT I) Sialylation
- An Integrated Control Strategy for the Fermentation of the Marine-Derived Fungus Aspergillus glaucus for the Production of Anti-cancer Polyketide被引量：20收藏
- 作者：Cai, Menghao Zhou, Xiangshan Lu, Jian Fan, Weimin Zhou, Jiushun Niu, Chuanpeng Kang, Li Sun, Xueqian Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Marine Biotechnology 2012
- 关键词：Biochemistry - Bioreactors - Shear stress - Aspergillus - Bioconversion - Metabolites - Sodium hydroxide - Diseases - Dissolved oxygen - Ketones
- Biosynthetic origin of the carbon skeleton of a novel anti-tumor compound, haloroquinone, from a marine-derived fungus, Halorosellinia sp.被引量：17收藏
- 作者：Niu, Chuanpeng Cai, Menghao Zhang, Yuanxing Zhou, Xiangshan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Letters 2012
- 关键词：Drug products - Ketones - Biosynthesis - Alkylation - Carbon - Musculoskeletal system
- Nutrition and bioprocess development for efficient biosynthesis of an antitumor compound from marine-derived fungus被引量：14收藏
- 作者：Zhou, Weiqiang Cai, Menghao Zhou, Jiushun Jiang, Tao Zhou, Jiao Wang, Meixia Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2013
- 关键词：Halorosellinia sp (no. 1403) Medium optimization 1403C Fermentation process Antitumor
- Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor被引量：13收藏
- 作者：Cai, Menghao Zhou, Xiangshan Lu, Jian Fan, Weimin Niu, Chuanpeng Zhou, Jiushun Sun, Xueqian Kang, Li Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2011
- 关键词：Oxygen - Turbomachine blades - Biomass - Bioconversion - Bioreactors - Turbine components - Impellers
- Improved production of the anticancer compound 1403C by glucose pulse feeding of marine Halorosellinia sp. (No. 1403) in submerged culture被引量：13收藏
- 作者：Kang, Li Cai, Menghao Yu, Chao Zhang, Yuanxing Zhou, Xiangshan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2011
- 关键词：Batch cell culture - Fungi
- Improved methanol-derived lovastatin production through enhancement of the biosynthetic pathway and intracellular lovastatin efflux in methylotrophic yeast被引量：13收藏
- 作者：Liu, Yiqi Bai, Chenxiao Xu, Qin Yu, Jiahui Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIORESOURCES AND BIOPROCESSING 2018
- 关键词：Enhanced biosynthetic pathway Lovastatin Methanol Pichia pastoris Statin pump protEIn
- Engineering substrate and energy metabolism for living cell production of cytidine-5′-diphosphocholine被引量：12收藏
- 作者：Ren, Yanna Liu, Qi Liu, Haifeng Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;China Resources Angde Biotech Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2020
- 关键词：ATP generation choline transport cytidine-5 '-diphosphocholine metabolic engineering Pichia pastoris
- A light-dark shift strategy derived from light-responded metabolic behaviors for polyketides production in marine fungus Halorosellinia sp.被引量：9收藏
- 作者：Zhang, Xiaoxu He, Hao Yin, Ying Zhou, Weiqiang Cai, Menghao Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：JOURNAL OF BIOTECHNOLOGY 2016
- 关键词：Fermentation Light regulation Light-dark shift Anticancer polyketide Halorosellinia sp. Marine fungus
- Mixed carbon source control strategy for enhancing alginate lyase production by marine Vibrio sp QY102被引量：8收藏
- 作者：Zhou, Jiushun Cai, Menghao Jiang, Tao Zhou, Weiqiang Shen, Wei Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2014
- 关键词：Alginate induction Alginate lyase Vibrio sp QY102 Medium optimization Mixed carbon source
- A comparative investigation on different refolding strategies of recombinant human tissue-type plasminogen activator derivative被引量：8收藏
- 作者：Liu, Haifeng Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shandong Dongeejiao Grp
- 来源：BIOTECHNOLOGY LETTERS 2006
- 关键词：inclusion body on-column refolding size-exclusion chromatography tissue-type plasminogen activator derivative
- PiggyBac transposon-mediated mutagenesis and application in yeast Komagataella phaffii被引量：7收藏
- 作者：Zhu, Jinxiang Zhu, Qiaoyun Gong, Ruiqing Xu, Qin Cai, Menghao Jiang, Tianyi Zhou, Xiangshan Zhou, Mian Zhang, Yuanxing
- 机构：East China Univ Sci & Technol;SCICB;Roche R&D Ctr China Ltd
- 来源：BIOTECHNOLOGY LETTERS 2018
- 关键词：Mutagenesis Komagataella phaffii piggyBac Transposon
