成果/Result
- The yeast peroxisome: A dynamic storage depot and subcellular factory for squalene overproduction被引量：194收藏
- 作者：Liu, Guo-Song Li, Tian Zhou, Wei Jiang, Min Tao, Xin-Yi Liu, Min Zhao, Ming Ren, Yu-Hong Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2020
- 关键词：Synthetic biology Saccharomyces cerevisiae Peroxisome Storage depot Subcellular factory Terpene
- Metabolic compartmentalization in yeast mitochondria: Burden and solution for squalene overproduction被引量：115收藏
- 作者：Zhu, Zhan-Tao Du, Meng-Meng Gao, Bei Tao, Xin-Yi Zhao, Ming Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2021
- 关键词：Saccharomyces cerevisiae Mitochondrial engineering Metabolic burden Squalene Dual engineering
- Characterization and engineering of 3-ketosteroid-Δ1-dehydrogenase and 3-ketosteroid-9α-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9α-hydroxy-4-androstene-3,17-dione through the catabolism of sterols被引量：99收藏
- 作者：Yao, Kang Xu, Li-Qin Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2014
- 关键词：3-Ketosteroid-Delta(1)-dehydrogenase Mycobacteria Sterols 9 alpha-Hydroxy-4-androstene-3,17-dione
- Metabolic Engineering of Saccharomyces cerevisiae To Overproduce Squalene被引量：71收藏
- 作者：Li, Tian Liu, Guo-Song Zhou, Wei Jiang, Min Ren, Yu-Hong Tao, Xin-Yi Liu, Min Zhao, Ming Wang, Feng-Qing Gao, Bei Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2020
- 关键词：Saccharomyces cerevisiae metabolic engineering squalene overproduction ethanol carbon source utilization
- Simple and Cheap CRISPR/Cas12a Biosensor Based on Plug-and-Play of DNA Aptamers for the Detection of Endocrine-Disrupting Compounds被引量：65收藏
- 作者：Wang, Feng-Qing Zhao, Yunqiu Zhu, Lin Ding, Yaxue Ji, Weiting Liu, Kun Liu, Ke Gao, Bei Tao, Xinyi Dong, Yu-Guo Wei, Dongzhi
- 机构： State Key Laboratory of Bioreactor Engineering; Key Laboratory of Biocatalysis and Intelligent Manufacturing
- 来源：SSRN 2023
- 关键词：DNA - Endocrine disrupters - Phenols
- Significantly Enhanced Production of Patchoulol in Metabolically Engineered Saccharomyces cerevisiae被引量：53收藏
- 作者：Ma, Bin Liu, Min Li, Zhen-Hai Tao, Xinyi Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：patchoulol sesquiterpene metabolic engineering Saccharomyces cerevisiae terpenoids
- Ammonia-containing dimethyl sulfoxide: An improved solvent for the dissolution of formazan crystals in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay被引量：51收藏
- 作者：Wang, Hengwei Wang, Fengqing Tao, Xinyi Cheng, Hairong
- 机构：Shanghai Jiao Tong Univ;E China Univ Sci & Technol
- 来源：ANALYTICAL BIOCHEMISTRY 2012
- 关键词：MTT assay Formazan Dimethyl sulfoxide Ammonia pH Viability
- Metabolic engineering of Pichia pastoris for the production of dammarenediol-II被引量：51收藏
- 作者：Liu, Xin-Bin Liu, Min Tao, Xin-Yi Zhang, Zhong-Xi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2015
- 关键词：Dammarenediol-II Metabolic engineering Pichia pastoris Ginsenosides 2,3-Oxidosqualene
- Establishment of an Efficient Expression and Regulation System in Streptomyces for Economical and High-Level Production of the Natural Blue Pigment Indigoidine被引量：46收藏
- 作者：Zhao, Ming Zhang, Xiu-Shan Xiong, Liang-Bin Liu, Kun Li, Xiang-Fei Liu, Yan Wang, Feng-Qing
- 机构： Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding; State Key Laboratory of Bioreactor Engineering; Shanghai Key Laboratory of Molecular Imaging
- 来源：Journal of Agricultural and Food Chemistry 2024
- 关键词：Amino acids - Bacteria - Condensation reactions - Metabolism
- New microbiological transformations of steroids by Streptomyces virginiae IBL-14被引量：46收藏
- 作者：Wang, Feng-Qing Zhang, Cheng-Gang Li, Bo Wei, Dong-Zhi Tong, Wang-Yu
- 机构： State Key Laboratory of Bioreactor Engineering; Integrated Biotechnology Laboratory
- 来源：Environmental Science and Technology 2009
- 关键词：Lipids - Metabolism - Waste treatment
- Impact of oxygen supply on rtPA expression in Escherichia coli BL21 (DE3): Ammonia effects被引量：45收藏
- 作者：Wang, Hengwei Wang, Fengqing Wei, Dongzhi
- 机构： Newworld Institute of Biotechnology
- 来源：Applied Microbiology and Biotechnology 2009
- 关键词：Bottles - Amino acids - Nitrogen - Cell growth - Growth kinetics - Recombinant protEIns - Ammonia - Batch cell culture - Ions - Oxygen supply
- Identification and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum被引量：39收藏
- 作者：Yao, Kang Wang, Feng-Qing Zhang, Huai-Cheng Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Metabolic Engineering 2013
- 关键词：Lipids - Alcohols
- Enhancing Biosynthesis of a Ginsenoside Precursor by Self-Assembly of Two Key Enzymes in Pichia pastoris被引量：36收藏
- 作者：Zhao, Chengcheng Gao, Xin Liu, Xinbin Wang, Yong Yang, Shengli Wang, Fengqing Ren, Yuhong
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2016
- 关键词：ginsenosides self-assembly Pichia pastoris dammarenediol-II
- A comparative study of β-1, 4-endoglucanase (possessing β-1, 4-exoglucanase activity) from Bacillus subtilis LH expressed in Pichia pastoris GS115 and Escherichia coli Rosetta (DE3)被引量：35收藏
- 作者：Zhao, Xi-Hua Wang, Wei Wang, Feng-Qing Wei, Dong-Zhi
- 机构： State Key Lab of Bioreactor Engineering; College of Life Science
- 来源：Bioresource Technology 2012
- 关键词：Yeast - Escherichia coli - Bacteriology
- Engineering Saccharomyces cerevisiae for Hyperproduction of β-Amyrin by Mitigating the Inhibition Effect of Squalene on β-Amyrin Synthase被引量：35收藏
- 作者：Du, Meng-Meng Zhu, Zhan-Tao Zhang, Ge-Ge Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Liu, Min Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：beta-amyrin squalene inhibition peroxisome compartmentalization competition pathway Saccharomyces cerevisiae
- Role Identification and Application of SigD in the Transformation of Soybean Phytosterol to 9α-Hydroxy-4-androstene-3,17-dione in Mycobacterium neoaurum被引量：30收藏
- 作者：Xiong, Liang-Bin Liu, Hao-Hao Xu, Li-Qin Wei, Dong-Zhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：mycobacteria 9a-hydroxy-4-androstene-3,17-dione (9-OHAD) soybean phytosterol transcription factor sigD choM2
- Engineered 3-ketosteroid 9a-hydroxylases in Mycobacterium neoaurum: An efficient platform for production of steroid drugs被引量：30收藏
- 作者：Liu, Hao-Hao Xu, Li-Qin Yao, Kang Xiong, Liang-Bin Tao, Xin-Yi Liu, Min Wang, Feng-Qing Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2018
- 关键词：Metabolic engineering - Lipids - Alcohols - Metabolism
- Characterization of P450 FcpC, the enzyme responsible for bioconversion of diosgenone to isonuatigenone in Streptomyces virginiae IBL-14被引量：29收藏
- 作者：Wang, Wei Wang, Feng-Qing Wei, Dong-Zhi
- 机构： State Key Lab. of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2009
- 关键词：Escherichia coli
- Characterization and engineering control of the effects of reactive oxygen species on the conversion of sterols to steroid synthons in Mycobacterium neoaurum被引量：29收藏
- 作者：Sun, Wan-Ju Wang, Li Liu, Hao-Hao Liu, Yong-Jun Ren, Yu-Hong Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Sterol catabolism Reactive oxygen species Catalase Mycothiol ErgothionEIne Mycobacteria
- Accumulation of androstadiene-dione by overexpression of heterologous 3-ketosteroid Δ1-dehydrogenase in Mycobacterium neoaurum NwIB-01被引量：29收藏
- 作者：Wei, Wei Fan, Shu-Yue Wang, Feng-Qing Wei, Dong-Zhi
- 机构：E China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2014
- 关键词：1,4-Androstadiene-3,17-dione 3-Ketosteroid Delta(1)-dehydrogenase 4-Androstene-3,17-dione Gene overexpression Phytosterols Plasmid stability
- Characterization and application of fusidane antibiotic biosynethsis enzyme 3-ketosteroid-Δ1-dehydrogenase in steroid transformation被引量：28收藏
- 作者：Chen, Miao-Miao Wang, Feng-Qing Lin, Liang-Cai Yao, Kang Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2012
- 关键词：Cytology - Escherichia coli - Amino acids - Steroid hormones - Biochemistry - Aspergillus - Cell membranes - Enzymes
- Inactivation and augmentation of the primary 3-Ketosteroid- δ1-Dehydrogenase in mycobacterium neoaurum NwIB-01: Biotransformation of soybean phytosterols to 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione被引量：27收藏
- 作者：Wei, Wei Wang, Feng-Qing Fan, Shu-Yue Wei, Dong-Zhi
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2010
- 关键词：Gene encoding - Polyols
- Enhanced itaconic acid production by self-assembly of two biosynthetic enzymes in Escherichia coli被引量：27收藏
- 作者：Yang, Zhongwei Gao, Xin Xie, Hui Wang, Fengqing Ren, Yuhong Wei, Dongzhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2017
- 关键词：itaconic acid aconitase cis-aconitate decarboxylase self-assembly protEIn-peptide interactions
- Increasing L-homoserine production in Escherichia coli by engineering the central metabolic pathways被引量：25收藏
- 作者：Liu, Min Lou, Jiali Gu, Jiali Lyu, Xiao-Mei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol;Huzhou Univ;Nanyang Technol Univ
- 来源：JOURNAL OF BIOTECHNOLOGY 2020
- 关键词：L-Homoserine Central metabolic pathways Metabolic engineering Escherichia coli
- Integrated Transcriptome and Proteome Studies Reveal the Underlying Mechanisms for Sterol Catabolism and Steroid Production in Mycobacterium neoaurum被引量：24收藏
- 作者：Liu, Min Xiong, Liang-Bin Tao, Xinyi Liu, Qing-Hai Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2018
- 关键词：sterol catabolism transcriptome proteome Mycobacterium
- A New Steroid-Transforming Strain of Mycobacterium neoaurum and Cloning of 3-Ketosteroid 9α-Hydroxylase in NwIB-01被引量：23收藏
- 作者：Wei, Wei Fan, Shuyue Wang, Fengqing Wei, Dongzhi
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Mycobacterium neoaurum Extraction chromatography Screening Phytosterols 4-Androstene-3,17-dione (AD) 1,4-Androstadiene-3,17-dione (ADD) 3-Ketosteroid 9 alpha-hydroxylase (KSH)
- Design and evaluation of a phospholipase D based drug delivery strategy of novel phosphatidyl-prodrug被引量：22收藏
- 作者：Tao, Xinyi Jia, Ning Cheng, Nenghui Ren, Yuhong Cao, Xuni Liu, Min Wei, Dongzhi Wang, Feng-Qing
- 机构：East China Univ Sci & Technol
- 来源：BIOMATERIALS 2017
- 关键词：Phosphatidyl-prodrug Phospholipase D Doxorubicin Triggered release Reverse multidrug resistance
- De novo design of a transcription factor for a progesterone biosensor被引量：20收藏
- 作者：Liu, Kun Zhang, Yunsen Liu, Ke Zhao, Yunqiu Gao, Bei Tao, Xinyi Zhao, Ming Wang, Feng-Qing Wei, Dongzhi
- 机构：East China Univ Sci & Technol;Chengdu Univ Tradit Chinese Med
- 来源：BIOSENSORS & BIOELECTRONICS 2022
- 关键词：Artificial transcription factor Molecular dynamics simulation Progesterone biosensor Whole-cell Diagnosis
- Multidimensional engineering of Saccharomyces cerevisiae for the efficient production of heme by exploring the cytotoxicity and tolerance of heme被引量：20收藏
- 作者：Guo, Qidi Li, Jiacun Wang, Ming-Rui Zhao, Ming Zhang, Gege Tang, Shuyan Xiong, Liang-Bin Gao, Bei Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：METABOLIC ENGINEERING 2024
- 关键词：Heme Saccharomyces cerevisiae Metabolic engineering Heme cytotoxicity Laboratory evolution
- Boosting the epoxidation of squalene to produce triterpenoids in Saccharomyces cerevisiae被引量：20收藏
- 作者：Du, Meng-Meng Zhang, Ge-Ge Zhu, Zhan-Tao Zhao, Yun-Qiu Gao, Bei Tao, Xin-Yi Wang, Feng-Qing Wei, Dong-Zhi
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 2023
- 关键词：2,3-Oxidosqualene 2,3:22,23-Dioxidosqualene Triterpenoid ERG7 Two-stage fermentation
