成果/Result
- Global regulator BldA regulates morphological differentiation and lincomycin production in Streptomyces lincolnensis被引量：46收藏
- 作者：Hou, Bingbing Tao, Liyuan Zhu, Xiaoyu Wu, Wei Guo, Meijin Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Applied Biology
- 来源：Applied Microbiology and Biotechnology 2018
- 关键词：Encoding (symbols) - Amino acids - Bacteria - Metabolism
- Cloning and characterization of the ddsA gene encoding decaprenyl diphosphate synthase from Rhodobacter capsulatus B10被引量：31收藏
- 作者：Liu, Xinyi Wu, Haizhen Ye, Jiang Yuan, Qinsheng Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Canadian Journal of Microbiology 2006
- 关键词：Amino acids - Bacteria - Cloning - Enzymes - Gene expression - Polypeptides
- Identification of BagI as a positive transcriptional regulator of bagremycin biosynthesis in engineered Streptomyces sp. Tü 4128被引量：30收藏
- 作者：Liu, Feng Xu, Dakui Zhang, Yuchen Zhu, Yunxia Ye, Jiang Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Microbiological Research 2015
- 关键词：Bacteria - Metabolism - Polymerase chain reaction - Fungi - Biochemistry
- Characterization of the bagremycin biosynthetic gene cluster in Streptomyces sp. Tü 4128被引量：22收藏
- 作者：Ye, Jiang Zhu, Yunxia Hou, Bingbing Wu, Haizhen Zhang, Huizhan
- 机构： Key Laboratory of Bioreactor Engineering; Department of Applied Biology
- 来源：Bioscience, Biotechnology and Biochemistry 2019
- 关键词：Antibiotics - Biochemistry - Bacteria - Genes
- Cloning and characterization of a novel tyrosine ammonia lyase-encoding gene involved in bagremycins biosynthesis in Streptomyces sp.被引量：19收藏
- 作者：Zhu, Yunxia Liao, Siyi Ye, Jiang Zhang, Huizhan
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2012
- 关键词：Aromatic amino acid ammonia lyase Bagremycins trans-coumaric acid Streptomyces Tyrosine ammonia lyase
- Developmental regulator RamRsl controls both morphological development and lincomycin biosynthesis in Streptomyces lincolnensis被引量：16收藏
- 作者：Wang, Ruida Cao, Yuan Kong, Fanjing Hou, Bingbing Zhao, Jiaqi Kang, Yajing Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF APPLIED MICROBIOLOGY 2022
- 关键词：lincomycin biosynthesis morphology RamR Streptomyces lincolnensis transcriptional regulation
- AflQ1-Q2 represses lincomycin biosynthesis via multiple cascades in Streptomyces lincolnensis被引量：15收藏
- 作者：Wang, Ruida Zhou, Tianyu Kong, Fanjing Hou, Bingbing Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2023
- 关键词：AflQ1-Q2 Streptomyces lincolnensis Two-component system Lincomycin biosynthesis Transcriptional regulation Aspartate metabolism
- ubiCA基因的强化表达及对大肠杆菌辅酶Q生物合成的影响被引量：15收藏
- 作者：叶江 马林 吴海珍 刘欣毅 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2006
- 关键词：大肠杆菌 ubiCA 基因克隆 强化表达 辅酶Q
- 摘要：为调查大肠杆菌高产辅酶Q(CoQ)的可能性,首先研究大肠杆菌生物合成关键基因ubiCA强化表达对大肠杆菌CoQ产量的影响。本文构建了含有ubiCA基因的5个表达质粒,将其分别转入E.coli JM 83或BL 21(DE...
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- Molecular cloning of an insertion sequence-like element from Vibrio anguillarum and its functional identification in E. coli被引量：13收藏
- 作者：Liang, Na Wu, Haizhen Ye, Jiang Xu, Peng Zhang, Yuanxing Zhang, Huizhan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Biotechnology Letters 2007
- 关键词：Cloning - Enzymes - Escherichia coli - Gene expression
- Characterization of a TetR-type positive regulator AtrA for lincomycin production in Streptomyces lincolnensis被引量：12收藏
- 作者：Wu, Wei Kang, Yajing Hou, Bingbing Ye, Jiang Wang, Ruida Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2023
- 关键词：Streptomyces lincolnensis AtrA lincomycin production regulatory pathway
- A novel method to identify yak fiber in textile被引量：12收藏
- 作者：Lu, Weimin Fei, Jing Yang, Juan Tang, Minfeng Dong, Zhenglian Zhou, Zhiping Ye, Jiang Wu, Haizhen
- 机构：Shanghai Entry Exit Inspect & Quarantine Bur;E China Univ Sci & Technol
- 来源：TEXTILE RESEARCH JOURNAL 2013
- 关键词：yak fiber deoxyribonuclEIc acid TaqMan-polymerase chain reaction identify
- LcbR1, a newly identified GntR family regulator, represses lincomycin biosynthesis in Streptomyces lincolnensis被引量：10收藏
- 作者：Wang, Ruida Zhao, Jiaqi Chen, Lei Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2023
- 关键词：Streptomyces lincolnensis LcbR1 GntR family Lincomycin biosynthesis Transcriptional regulation
- DeoR regulates lincomycin production in Streptomyces lincolnensis被引量：10收藏
- 作者：Zou, Jingyun Mao, Yue Hou, Bingbing Kang, Yajing Wang, Ruida Wu, Haizhen Ye, Jiang Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2023
- 关键词：Streptomyces lincolnensis Lincomycin DeoR family Transcriptional regulation
- 林可霉素生物合成基因lmbU的敲除及其遗传回补被引量：9收藏
- 作者：吕彩霞 杨捷 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：同源重组 敲除 遗传回补 林可霉素生物合成
- 摘要：利用DNA同源重组原理敲除S.lincolnensisNRRL 2936中lmbU基因,筛选获得该基因缺陷株S.lincolnensisJLUa2,其不具备林可霉素生物合成能力。利用构建的不同重组质粒转化缺陷株,发现含有...
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- 基因替换及多基因共表达强化大肠杆菌辅酶Q_(10)的合成能力被引量：8收藏
- 作者：傅楠 叶江 吴海珍 孔璐 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：大肠杆菌 基因缺失 多基因共表达 辅酶Q
- 摘要：为了强化大肠杆菌合成辅酶Q10(CoQ10)的能力,对大肠杆菌进行了相关的基因操作。通过敲除大肠杆菌染色体上的聚八异戊二烯焦磷酸合成酶基因ispB,并导入来自Gluconobactersuboxydans的聚十异戊二烯焦...
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- Cloning and expression of the polyhydroxyalkanote depolymerase gene from Pseudomonas putida, and characterization of the gene product被引量：8收藏
- 作者：Jiang, Yaqin Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY LETTERS 2004
- 关键词：cloning depolymerase polyhydroxyalkanote Pseudomonas
- Cloning and identification of the Frigocyclinone biosynthetic gene cluster from Streptomyces griseus strain NTK 97被引量：7收藏
- 作者：Mo, Jian Ye, Jiang Chen, Haozhe Hou, Bingbing Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 2019
- 关键词：Biosynthesis frigocyclinone type II polyketide synthases C-glycosyltransferase CRISPR-Cpf1
- MarR family regulator LcbR2 activates lincomycin biosynthesis in multiple ways被引量：5收藏
- 作者：Wang, Ruida Chen, Lei Zhao, Jiaqi Gao, Yu Zhou, Tianyu Ye, Jiang Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Tarim Univ
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2025
- 关键词：Streptomyces lincolnensis LcbR2 MarR family Lincomycin biosynthesis Transcriptional regulator
- 红色糖多孢菌红霉素抗性基因的克隆与鉴定被引量：3收藏
- 作者：孙丽萍 沈琼 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2002
- 关键词：鸟枪法 原生质体转化 红色糖多孢菌 红霉素抗性基因 双重抗性筛选 基因克隆 基因鉴定
- 摘要：将红色糖多孢菌 ( Saccharopolyspora erythraea)的染色体 DNA用 Pst I酶切后与载体p UWL2 0 1连接 ,转化变铅青链霉菌 ( S.lividans) TK2 3的原生质体。采用双...
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- 大肠杆菌巴豆甜菜碱还原酶基因缺失菌株的构建被引量：1收藏
- 作者：张文刚 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2004
- 关键词：同源重组 巴豆甜菜碱还原酶 基因缺失 抗性基因 突变株
- 摘要：报道了体外构建caiA基因缺失的带有卡那霉素抗性基因的5.2kb线状DNA分子,以此转化大肠杆菌JM83和BL21(DE3)株,借助于体内DNA同源重组,定向敲除了大肠杆菌中的巴豆甜菜碱还原酶编码基因caiA。经遗传稳定...
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- Metabolic engineering of Bacillus subtilis for high-yield surfactin production被引量：0收藏
- 作者：Wang, Yong Li, Hanqing Mao, Yue Li, Pengfei Wu, Haizhen Ye, Jiang Zhang, Huizhan
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 2026
- 关键词：Surfactin Bacillus subtilis Metabolic engineering P43 promoter Tolerance engineering
- Revealing and manipulating the LmbU-mediated connection between lincomycin production and the ectABCD gene cluster in S. lincolnensis被引量：0收藏
- 作者：Mao, Yue Ye, Jiang Wang, Ruida Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;Tarim Univ
- 来源：PROCESS BIOCHEMISTRY 2026
- 关键词：Streptomyces lincolnensis Lincomycin LmbU Ectoine/hydroxyectoine Osmotic regulation
- Inception-level signal amplification: Cascaded DNAzyme-Cas9 nickase achieves sub-nanomolar kanamycin tracking被引量：0收藏
- 作者：Xu, Wen Cheng, Yutian Sun, Kexin Wu, Yue Xu, Yiming Ye, Jiang Li, Pengfei Wu, Haizhen
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ANALYTICA CHIMICA ACTA 2026
- 关键词：Kanamycin Aptamer DNAzyme Cas9 nickase
- Characterization and functional analysis of FruR in Streptomyces lincolnensis: A plEIotropic regulator that links fructose metabolism to lincomycin biosynthesis被引量：0收藏
- 作者：Gao, Yu Chen, Lei Zhang, Zhicheng Xu, Nuo Ye, Jiang Wang, Ruida Wu, Haizhen Zhang, Huizhan
- 机构：East China Univ Sci & Technol;Tarim Univ
- 来源：JOURNAL OF BIOTECHNOLOGY 2026
- 关键词：FruR Fructose metabolism Lincomycin biosynthesis Streptomyces lincolnensis Transcriptional regulator
- 链霉菌辅酶Q9生物合成基因sdsA的鉴定被引量：0收藏
- 作者：季岷岚 李琳君 叶江 吴海珍 张惠展
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2008
- 关键词：sdsA 聚异戊二烯焦磷酸合成酶 链霉菌 辅酶Q
- 摘要：聚异戊二烯焦磷酸合成酶基因在4个不同种的链霉菌(Streptomyces coelicolorA3(2),Streptomyces maritimus,Streptomyces mycarofaciensATCC 214...
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- 大肠杆菌guaC基因的克隆及高效表达被引量：0收藏
- 作者：糜蓉娟 吴海珍 叶江 张惠展 叶勤
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2003
- 关键词：guaC E.coli MC4100 鸟苷酸还原酶 基因表达
- 摘要：以 E.coli MC41 0 0染色体 DNA为模板使用 PCR扩增技术克隆了鸟苷酸还原酶基因gua C,该基因全长 1 0 44bp,编码相对分子质量为 3 7ku的蛋白质。将该基因直接克隆于 p ET-1 6b质粒...
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