成果/Result
- Effects of exogenous GABA on gene expression of Caragana intermedia roots under NaCl stress: regulatory roles for H2O2 and ethylene production被引量：152收藏
- 作者：Shi, Sheng-Qing Shi, Zheng Jiang, Ze-Ping Qi, Li-Wang Sun, Xiao-Mei Li, Chun-Xiu Liu, Jian-Feng Xiao, Wen-Fa Zhang, Shou-Gong
- 机构：Chinese Acad Forestry;Chinese Acad Forestry;E China Univ Sci & Technol
- 来源：PLANT CELL AND ENVIRONMENT 2010
- 关键词：suppression subtractive hybridization gene expression signal transduction reactive oxygen species nitrogen sources proline
- Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor被引量：129收藏
- 作者：Wang, Li-Juan Li, Chun-Xiu Ni, Yan Zhang, Jie Liu, Xiang Xu, Jian-He
- 机构：E China Univ Sci & Technol;Jiangnan Univ
- 来源：BIORESOURCE TECHNOLOGY 2011
- 关键词：Carbonyl reductase Streptomyces coelicolor Asymmetric reduction Ethyl (S)-4-chloro-3-hydroxybutanoate NADH regeneration
- Reshaping the Active Pocket of Amine Dehydrogenases for Asymmetric Synthesis of Bulky Aliphatic Amines被引量：102收藏
- 作者：Chen, Fei-Fei Zheng, Gao-Wei Liu, Lei Li, Hao Chen, Qi Li, Fu-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2018
- 关键词：amine dehydrogenase reductive amination chiral amine protein engineering biocatalysis substrate scope
- Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1被引量：82收藏
- 作者：Zheng, Gao-Wei Liu, Yuan-Yang Chen, Qi Huang, Lei Yu, Hui-Lei Lou, Wen-Yong Li, Chun-Xiu Bai, Yun-Peng Li, Ai-Tao Xu, Jian-He
- 机构：East China Univ Sci & Technol;South China Univ Technol;Max Planck Inst Kohlenforsch
- 来源：ACS CATALYSIS 2017
- 关键词：asymmetric reduction ketoreductase chiral alcohol protein engineering substrate specificity biocatalysis
- Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity被引量：68收藏
- 作者：Ni, Yan Li, Chun-Xiu Ma, Hong-Min Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：YtbE Recombinant aldo-keto reductase Bacillus sp. Asymmetric reduction Chiral alcohols
- Switching Cofactor Dependence of 7β-Hydroxysteroid Dehydrogenase for Cost-Effective Production of Ursodeoxycholic Acid被引量：66收藏
- 作者：You, Zhi-Neng Chen, Qi Shi, Shou-Cheng Zheng, Ming-Min Pan, Jiang Qian, Xiao-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis cofactor dependence small-and-smart library 7 beta-hydroxysteroid dehydrogenase molecular dynamics simulation ursodeoxycholic acid
- Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques被引量：66收藏
- 作者：Zheng, Ming-Min Wang, Ru-Feng Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;China Pharmaceut Univ
- 来源：PROCESS BIOCHEMISTRY 2015
- 关键词：Ursodeoxycholic acid Chenodeoxycholic acid 7 alpha-Hydroxysteroid dehydrogenase 7 beta-Hydroxysteroid dehydrogenase Two-step reaction
- Efficient Synthesis of a Chiral Precursor for Angiotensin-Converting Enzyme (ACE) Inhibitors in High Space-Time Yield by a New Reductase without External Cofactors被引量：61收藏
- 作者：Shen, Nai-Dong Ni, Yan Ma, Hong-Min Wang, Li-Juan Li, Chun-Xiu Zheng, Gao-Wei Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC LETTERS 2012
- Stereospecific Reduction of Methyl o-Chlorobenzoylformate at 300 g?L-1 without Additional Cofactor using a Carbonyl Reductase Mined from Candida glabrata被引量：57收藏
- 作者：Ma, Hongmin Yang, Linlin Ni, Yan Zhang, Jie Li, Chun-Xiu Zheng, Gao-Wei Yang, Huaiyu Xu, Jian-He
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2012
- 关键词：bioinformatics biotransformations cofactors oxidoreductases
- Engineering 7β-Hydroxysteroid Dehydrogenase for Enhanced Ursodeoxycholic Acid Production by Multiobjective Directed Evolution被引量：57收藏
- 作者：Zheng, Ming-Min Chen, Ke-Cai Wang, Ru-Feng Li, Hao Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：biocatalysis cascade reaction 7 beta-hydroxysteroid dehydrogenase multiobjective directed evolution ursodeoxycholic acid
- Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata被引量：53收藏
- 作者：Zhang, Chen-Sheng Zhang, Zhi-Jun Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2012
- 关键词：Nitrilase Labrenzia aggregate Enantioselective hydrolysis (R)-o-Chloromandelic acid Biphasic system
- Efficient Reduction of Ethyl 2-Oxo-4-phenylbutyrate at 620 g.L-1 by a Bacterial Reductase with Broad Substrate Spectrum被引量：50收藏
- 作者：Ni, Yan Li, Chun-Xiu Zhang, Jie Shen, Nai-Dong Bornscheuer, Uwe T. Xu, Jian-He
- 机构：E China Univ Sci & Technol;Ernst Moritz Arndt Univ Greifswald
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2011
- 关键词：alcohols asymmetric catalysis cofactor oxidoreductases reduction
- Highly stereoselective reduction of prochiral ketones by a bacterial reductase coupled with cofactor regeneration被引量：46收藏
- 作者：Ni, Yan Li, Chun-Xiu Wang, Li-Juan Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC & BIOMOLECULAR CHEMISTRY 2011
- 关键词：Aromatic compounds - Bacteriology - Enantiomers - Ketones
- Crosslinking of enzyme coaggregate with polyethyleneimine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase被引量：35收藏
- 作者：Pan, Jiang Kong, Xu-Dong Li, Chun-Xiu Ye, Qin Xu, Jian-He Imanaka, Tadayuki
- 机构：E China Univ Sci & Technol;Ritsumeikan Univ
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：Crosslinked enzyme coaggregate Serratia marcescens lipase Polyethyleneimine Coaggregation of enzyme trans-3-(4 '-Methoxyphenyl)glycidic acid methyl ester (MPGM)
- Evolution of Glucose Dehydrogenase for Cofactor Regeneration in Bioredox Processes with Denaturing Agents被引量：35收藏
- 作者：Qian, Wen-Zhuo Ou, Ling Li, Chun-Xiu Pan, Jiang Xu, Jian-He Chen, Qi Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2020
- 关键词：biocatalysis chemical stability cofactor regeneration directed evolution glucose dehydrogenase
- Enzymatic synthesis of high-titer nicotinamide mononucleotide with a new nicotinamide riboside kinase and an efficient ATP regeneration system被引量：34收藏
- 作者：Qian, Xiao-Long Dai, Yi-Si Li, Chun-Xiu Pan, Jiang Xu, Jian-He Mu, Bozhong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Enzymatic phosphorylation Nicotinamide riboside Nicotinamide riboside kinase beta-Nicotinamide mononucleotide (NMN) ATP regeneration system
- Continuous Production of Ursodeoxycholic Acid by Using Two Cascade Reactors with Co-immobilized Enzymes被引量：33收藏
- 作者：Zheng, Ming-Min Chen, Fei-Fei Li, Hao Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2018
- 关键词：domino reactions enzymes immobilization natural products supported catalysts
- An efficient bioprocess for enzymatic production of L-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli被引量：31收藏
- 作者：Zheng, Gao-Wei Pan, Jiang Yu, Hui-Lei Ngo-Thi, Minh-Thu Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2010
- 关键词：Whole-cell biocatalysis High ratio of substrate to catalyst High substrate loading Esterase L-Menthol
- De Novo Multienzyme Synthetic Pathways for Lactic Acid Production被引量：31收藏
- 作者：Ding, Xu-Wei Rong, Jing Pan, Ze-Peng Zhu, Xin-Xin Zhu, Zhen-Yu Chen, Qi Zhang, Zhi-Jun Xu, Jian-He Li, Chun-Xiu Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2024
- 关键词：biocatalysis C1 compound lacticacid multienzyme cascade protein engineering
- A Novel (R)-Imine Reductase from Paenibacillus lactis for Asymmetric Reduction of 3H-Indoles被引量：29收藏
- 作者：Li, Hao Zhang, Guang-Xiang Li, Liu-Mei Ou, Yu-Shi Wang, Ming-Yang Li, Chun-Xiu Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CHEMCATCHEM 2016
- 关键词：asymmetric reduction biocatalysis imine reductase indoles indolines
- A new thermostable β-glucosidase mined from Dictyoglomus thermophilum: Properties and performance in octyl glucoside synthesis at high temperatures被引量：28收藏
- 作者：Zou, Zheng-Zheng Yu, Hui-Lei Li, Chun-Xiu Zhou, Xin-Wen Hayashi, Chihiro Sun, Jie Liu, Bao-Hong Imanaka, Tadeyuki Xu, Jian-He
- 机构：E China Univ Sci & Technol;Fudan Univ;Fudan Univ;Ritsumeikan Univ
- 来源：BIORESOURCE TECHNOLOGY 2012
- 关键词：beta-Glucosidase Dictyoglomus thermophilum Thermostability Octyl glucoside Reverse hydrolysis
- Remodeling the Homologous Recombination Mechanism of Yarrowia lipolytica for High-Level Biosynthesis of Squalene被引量：26收藏
- 作者：Xu, Man Yang, Nan Pan, Jiang Hua, Qiang Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2024
- 关键词：homologous recombination squalene Yarrowia lipolytica metabolic engineering acetyl coenzyme A
- Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of DL-menthyl acetate被引量：26收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Li, Chun-Xiu Pan, Jiang Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2011
- 关键词：Bacillus subtilis esterase Cross-linked enzyme aggregates Hydrolysis Immobilization L-Menthol
- Combinatorial Evolution of Phosphotriesterase Toward a Robust Malathion Degrader by Hierarchical Iteration Mutagenesis被引量：25收藏
- 作者：Luo, Xiao-Jing Zhao, Jian Li, Chun-Xiu Bai, Yun-Peng Reetz, Manfred T. Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Max Planck Inst Kohlenforsch;Philipps Univ Marburg
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2016
- 关键词：biocatalysis directed evolution DNA shuffling malathion phosphotriesterase
- Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp nitrilase被引量：24收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2014
- 关键词：Immobilized cells Alcaligenes sp nitrilase Glutaraldehyde cross-linking Enantioselective hydrolysis (R)-(-)-Mandelic acid
- Catalytic Performance of Corn Stover Hydrolysis by a New Isolate Penicillium sp ECU0913 Producing both Cellulase and Xylanase被引量：24收藏
- 作者：Shi, Qian-Qian Sun, Jie Yu, Hui-Lei Li, Chun-Xiu Bao, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Penicillium sp. Cellulase Xylanase Enzymatic hydrolysis Corn stover
- Cloning of Thermostable Cellulase Genes of Clostridium thermocellum and Their Secretive Expression in Bacillus subtilis被引量：24收藏
- 作者：Liu, Jian-Ming Xin, Xiu-Juan Li, Chun-Xiu Xu, Jian-He Bao, Jie
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2012
- 关键词：Clostridium thermocellum Bacillus subtilis pP43JM2 Cellulase genes Synergy
- Burkholderia jiangsuensis sp nov., a methyl parathion degrading bacterium, isolated from methyl parathion contaminated soil被引量：23收藏
- 作者：Liu, Xu-Yun Li, Chun-Xiu Luo, Xiao-Jing Lai, Qi-Liang Xu, Jian-He
- 机构：E China Univ Sci & Technol;State Key Lab Breeding Base Marine Genet Resource;SOA;Key Lab Marine Genet Resources Fujian Prov
- 来源：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 2014
- Efficient Synthesis of 12-Oxochenodeoxycholic Acid Using a 12α-Hydroxysteroid Dehydrogenase from Rhodococcus ruber被引量：23收藏
- 作者：Shi, Shou-Cheng You, Zhi-Neng Zhou, Ke Chen, Qi Pan, Jiang Qian, Xiao-Long Xu, Jian-He Li, Chun-Xiu
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2019
- 关键词：biocatalysis 12 alpha-hydroxysteroid dehydrogenase NAD(+)-dependence structure-guided genome mining 12-oxochenodeoxycholic acid
- Bioreduction of methyl o-chlorobenzoylformate at 500 g L-1 without external cofactors for efficient production of enantiopure clopidogrel intermediate被引量：22收藏
- 作者：Ni, Yan Pan, Jiang Ma, Hong-Min Li, Chun-Xiu Zhang, Jie Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：TETRAHEDRON LETTERS 2012
- 关键词：Asymmetric reduction Aldo-keto reductase Whole-cell catalyst Methyl (R)-o-chloromandelate Clopidogrel
