- Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol被引量：123收藏
- 作者：Liu, Yiqi Tu, Xiaohu Xu, Qin Bai, Chenxiao Kong, Chuixing Liu, Qi Yu, Jiahui Peng, Qiangqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Methanol Methylotrophic yeast Co-culture Heterologous biosynthesis Monacolin J Lovastatin
- 质疑
- CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris被引量：89收藏
- 作者：Liu, Qi Shi, Xiaona Song, Lili Liu, Haifeng Zhou, Xiangshan Wang, Qiyao Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Chinare Resources Angde Biotech Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：MICROBIAL CELL FACTORIES 2019
- 关键词：Pichia pastoris CRISPR-Cas9 Homology directed repair Multiloci integration Multistep enzymatic pathway
- 质疑
- Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast被引量：45收藏
- 作者：Liu, Yiqi Bai, Chenxiao Liu, Qi Xu, Qin Qian, Zhilan Peng, Qiangqiang Yu, Jiahui Xu, Mingqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Ethanol Crabtree-negative yeast Acetyl-CoA Monacolin J Metabolic engineering Coculture
- 质疑
- A programmable high-expression yeast platform responsive to user-defined signals被引量：35收藏
- 作者：Liu, Qi Song, Lili Peng, Qiangqiang Zhu, Qiaoyun Shi, Xiaona Xu, Mingqiang Wang, Qiyao Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SCIENCE ADVANCES 2022
- 关键词：Recombinant proteins - Transcription
- 质疑
- de Novo Production of Plant 4′-deoxyflavones Baicalein and Oroxylin A from Ethanol in Crabtree-Negative Yeast被引量：32收藏
- 作者：Qian, Zhilan Yu, Jiahui Chen, Xinjie Kang, Yijia Ren, Yanna Liu, Qi Lu, Jian Zhao, Qing Cai, Menghao
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Chinese Acad Sci;East China Univ Sci & Technol
- 来源：ACS SYNTHETIC BIOLOGY 2022
- 关键词：plant 4 '-deoxyflavones Scutellaria baicalensis Georgi Pichia pastoris transcriptional regulation device fine-tuning pathway control
- 质疑
- Synthesis of core-shell molecularly imprinted polymers (MIP) for spiramycin I and their application in MIP chromatography被引量：29收藏
- 作者：Liu, Qi Wan, Junfen Cao, Xuejun
- 机构：East China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2018
- 关键词：Spiramycin I Core-shell molecularly imprinted polymers Selectivity Chromatographic column
- 质疑
- Coproduction of single cell protein and lipid from lignocellulose derived carbohydrates and inorganic ammonia salt with soluble ammonia recycling被引量：26收藏
- 作者：Zhang, Bin Ren, Dayu Liu, Qi Liu, Xiucai Bao, Jie
- 机构：East China Univ Sci & Technol;Cathay Biotech Inc
- 来源：BIORESOURCE TECHNOLOGY 2023
- 关键词：Single cell protein Microbial lipid Lignocellulose Trichosporon cutaneum Wastewater
- 质疑
- Heterologous pathway assembly reveals molecular steps of fungal terreic acid biosynthesis被引量：26收藏
- 作者：Kong, Chuixing Huang, Hezhou Xue, Ying Liu, Yiqi Peng, Qiangqiang Liu, Qi Xu, Qin Zhu, Qiaoyun Yin, Ying Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SCIENTIFIC REPORTS 2018
- 质疑
- Ultra-centrifugation force in adaptive evolution changes the cell structure of oleaginous yeast Trichosporon cutaneum into a favorable space for lipid accumulation被引量：16收藏
- 作者：Liu, Qi Lu, Minping Jin, Ci Hou, Weiliang Zhao, Liao Bao, Jie
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2022
- 关键词：adaptive evolution cell structure lignocellulose microbial lipid oleaginous yeast ultra-centrifugation
- 质疑
- Engineered dynamic distribution of malonyl-CoA flux for improving polyketide biosynthesis in Komagataella phaffii被引量：15收藏
- 作者：Wen, Jiao Tian, Lin Liu, Qi Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：JOURNAL OF BIOTECHNOLOGY 2020
- 关键词：Komagataella phaffii FapR/fapO Malonyl-CoA Dynamic control Synthetic biology
- 质疑
- Engineering substrate and energy metabolism for living cell production of cytidine-5′-diphosphocholine被引量：12收藏
- 作者：Ren, Yanna Liu, Qi Liu, Haifeng Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;China Resources Angde Biotech Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2020
- 关键词：ATP generation choline transport cytidine-5 '-diphosphocholine metabolic engineering Pichia pastoris
- 质疑
- de novo transcriptome sequencing of marine-derived Aspergillus glaucus and comparative analysis of metabolic and developmental variations in response to salt stress被引量：12收藏
- 作者：Liu, Shaomei Li, Jiaxin Wu, Yuan Ren, Yanna Liu, Qi Wang, Qiyao Zhou, Xiangshan Cai, Menghao Zhang, Yuanxing
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：GENES & GENOMICS 2017
- 关键词：Marine fungi Aspergillus glaucus Salt stress RNA-Seq Asexual development Secondary metabolites
- 质疑
- Yeast transcriptional device libraries enable precise synthesis of value-added chemicals from methanol被引量：12收藏
- 作者：Zhu, Qiaoyun Liu, Qi Yao, Chaoying Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;East China Univ Sci & Technol
- 来源：NUCLEIC ACIDS RESEARCH 2022
- 质疑
- 非常规酵母天然产物合成被引量：9收藏
- 作者：钱芷兰 宋丽丽 刘启 龚秀龙 康艺嘉 何子雨 龙浩雨 蔡孟浩
- 机构：华东理工大学生物工程学院;华东理工大学生物反应器工程国家重点实验室
- 来源：《生物工程学报》 2023
- 关键词：天然产物 非常规酵母 细胞工厂 代谢工程
- 摘要：以解脂耶氏酵母(Yarrowia lipolytica)、巴斯德毕赤酵母(Pichia pastoris)、马克斯克鲁维酵母(Kluyveromyces marxianus)、圆红冬孢酵母(Rhodosporidium ...
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- 巴斯德毕赤酵母底盘细胞的工程化改造及应用被引量：8收藏
- 作者：刘启 钱芷兰 宋丽丽 要超颖 徐名强 任燕娜 蔡孟浩
- 机构：华东理工大学生物工程学院
- 来源：《合成生物学》 2022
- 关键词：毕赤酵母 底盘细胞 甲基营养型酵母 细胞工厂 合成生物系统
- 摘要：优质的微生物底盘宿主是实现绿色、可持续生物制造的重要平台。巴斯德毕赤酵母底盘宿主因其在蛋白表达和发酵生产中的诸多优势受到了广泛的关注和应用。而作为一种工业甲基营养酵母,其可以有效地利用来源广泛的甲醇作为唯一碳源,使其成为...
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- Cellulase mediated stress triggers the mutations of oleaginous yeast Trichosporon cutaneum with super-large spindle morphology and high lipid accumulation被引量：8收藏
- 作者：Liu, Qi Li, Yuanyuan Hou, Weiliang Zhang, Bin Bao, Jie
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY JOURNAL 2023
- 关键词：cell size cell wall structure cellulase microbial lipid oleaginous yeast
- 质疑
- High-level living cell production of cytidine-5′-diphosphocholine in metabolically engineered yeast被引量：7收藏
- 作者：Ren, Yanna Liu, Qi Liu, Haifeng Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;China Resources Angde Biotech Pharma Co Ltd;China Resources Biopharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：JOURNAL OF BIOTECHNOLOGY 2021
- 关键词：Living cell production Metabolic engineering Cytidine-5 '-diphosphocholine Pichia pastoris
- 质疑
- Simultaneous enhancement of lignin-derived inhibitor tolerance and lipid accumulation of oleaginous yeast Trichosporon cutaneum by adaptive evolution被引量：7收藏
- 作者：Liu, Qi Zhang, Bin Hu, Mingshan Bao, Jie
- 机构：East China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2024
- 关键词：Lignocellulose Biolipid Oleaginous yeast Inhibitors Adaptive evolution
- 质疑
- de novo biosynthesis and nicotinamide biotransformation of nicotinamide mononucleotide by engineered yeast cells被引量：7收藏
- 作者：Ren, Yanna Han, Bei Wang, Shijie Wang, Xingbin Liu, Qi Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met
- 来源：MICROBIAL BIOTECHNOLOGY 2024
- 质疑
- Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris被引量：6收藏
- 作者：Liu, Qi He, Ziyu Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY 2025
- 关键词：Pichia pastoris Methanol metabolism Transcriptome analysis Transcription activator AOX1 promoter
- 质疑
- 毕赤酵母底盘芳香族氨基酸合成途径改造生产肉桂酸及对香豆酸被引量：5收藏
- 作者：陈鑫洁 钱芷兰 刘启 赵清 张元兴 蔡孟浩
- 机构：华东理工大学生物反应器工程国家重点实验室;中国科学院上海辰山植物科学研究中心上海市资源植物功能基因组学重点实验室
- 来源：《中国生物工程杂志》 2021
- 关键词：毕赤酵母 芳香族氨基酸 肉桂酸 对香豆酸
- 摘要：目的:改造毕赤酵母使其异源合成类黄酮生物合成途径的重要中间体肉桂酸、对香豆酸,并优化前体芳香族氨基酸生物合成途径以提高毕赤酵母的生产能力。方法:在毕赤酵母GS115中利用乙醇诱导型人工转录系统表达Rhodotorula ...
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- Recycling of waste calcium carbonate in lignocellulosic biorefining chain for chiral lactic acid production被引量：4收藏
- 作者：Zhang, Bin Han, Qingmei Liu, Qi Wang, Ya Bao, Jie
- 机构：East China Univ Sci & Technol;Shihezi Univ
- 来源：BIORESOURCE TECHNOLOGY 2024
- 关键词：Lignocellulose Biorefinery Solid residues Ash Neutralization
- 质疑
- Nucleotide distribution analysis of 5′UTRs in genome-scale directs their redesign and expression regulation in yeast被引量：4收藏
- 作者：Yao, Chaoying Yin, Yu Li, Qingyang Zhang, Hanqi Zhang, Yilun Shao, Qianqian Liu, Qi Ren, Yanna Cai, Menghao
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2025
- 关键词：Gene expression regulation - Nucleotides - Transcription
- 质疑
- Rational design and characterization of enhanced alcohol-inducible synthetic promoters in Pichia pastoris被引量：3收藏
- 作者：Liu, Qi Li, Yun-hao Tao, Liu-fei Yang, Jia-yi Zhang, Yi-lun Cai, Meng-hao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2025
- 关键词：Pichia pastoris methanol ethanol synthetic promoter
- 质疑
- Engineered Folding and Secretion for Expression Improvement of α-Amylase in Komagataella Phaffii被引量：3收藏
- 作者：Zhang, Yilun Wang, Xingbin Han, Bei Yao, Chaoying Liu, Qi Cai, Menghao
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：FOOD BIOENGINEERING 2025
- 关键词：K. phaffii molecular chaperone multicopy vesicle transport factor alpha-amylase
- 质疑
- 毕赤酵母表达全长单克隆抗体的设计及优化被引量：1收藏
- 作者：叶凯雄 龚秀龙 徐名强 钱芷兰 刘启 蔡孟浩
- 机构：华东理工大学生物工程学院生物反应器国家重点实验室
- 来源：《中国生物制品学杂志》 2025
- 关键词：单克隆抗体 毕赤酵母 分子伴侣
- 摘要：目的利用毕赤酵母底盘宿主表达单克隆抗体,并探索多种表达优化策略以提高抗体产量。方法以毕赤酵母为底盘细胞,用甲醇诱导型系统PAOX1和乙醇诱导型系统E1分别尝试多种单克隆抗体蛋白的重组表达,并通过分子伴侣过表达和培养基组分...
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- Heterologous Expression and Functional Characterization of a Truncated Marine Alginate Lyase被引量：1收藏
- 作者：Shao, Qianqian Yao, Chaoying Wang, Xingbin Han, Bei Liu, Qi Cai, Menghao
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2025
- 关键词：Alginate lyase
Komagataella phaffii Non-catalytic domain truncation Enzymatic property Heterologous expression - 质疑
- Biosynthesis of plant vitexin-derived flavonoid glycosides in yeast被引量：1收藏
- 作者：Qian, Zhilan Yu, Haishuang Song, Chenglin Zhang, Yang Liu, Qi Qu, Xudong Ye, Min Qiao, Xue Cai, Menghao
- 机构：East China Univ Sci & Technol;Peking Univ;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ
- 来源：NATURE SYNTHESIS 2026
- 质疑
- Programmable and controllable sexual life cycle for improved evolution in Komegataella phaffii被引量：0收藏
- 作者：Song, Lili Wu, Yanyan Hua, Li Liu, Qi Li, Yunhao Zhou, Xiangshan Cai, Menghao
- 机构：East China Univ Sci & Technol;China Resources Biopharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2026
- 关键词：Cell proliferation - Life cycle
- 质疑
- Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators in Pichia pastoris被引量：0收藏
- 作者：Qi Liu Ziyu He Menghao Cai
- 机构：State Key Laboratory of Bioreactor Engineering;Shanghai Collaborative Innovation Center for Biomanufacturing
- 来源：合成和系统生物技术(英文) 2025
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