周祥山 收藏
( 任职于 生物工程学院)
生物化工专业：博士生/硕士生导师 生物工程专业：博士生/硕士生导师 生物工程领域：工程硕士生导师 男，博士，华东理工大学生物工程学院，生物反应器工程国家重点实验室教授，博士生导师；东阿阿胶股份有限公司副总裁、国家胶类中药工程技术研究中心主任。 1974年9月生，2002年获华东理工大学生物化工博士学位，2014年获中欧国际工商学院工商管理硕士学位（EMBA）。2001年留校华东理工大学生物工程学院，生物反应器工程国家重点实验室工作。2004年升副教授，2008年德国卡尔斯鲁尔大学访问学者，2009年升教授，博士生... 详细>>
- Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shift Induction in Pichia pastoris被引量：136收藏
- 作者：Wang, Jinjia Wang, Xiaolong Shi, Lei Qi, Fei Zhang, Ping Zhang, Yuanxing Zhou, Xiangshan Song, Zhiwei Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg;Bioproc Technol Inst;Natl Univ Singapore
- 来源：SCIENTIFIC REPORTS 2017
- 质疑
- Engineered monoculture and co-culture of methylotrophic yeast for de novo production of monacolin J and lovastatin from methanol被引量：123收藏
- 作者：Liu, Yiqi Tu, Xiaohu Xu, Qin Bai, Chenxiao Kong, Chuixing Liu, Qi Yu, Jiahui Peng, Qiangqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Methanol Methylotrophic yeast Co-culture Heterologous biosynthesis Monacolin J Lovastatin
- 质疑
- Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris被引量：95收藏
- 作者：Wang, Xiaolong Wang, Qi Wang, Jinjia Bai, Peng Shi, Lei Shen, Wei Zhou, Mian Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：E China Univ Sci & Technol;SCICB
- 来源：JOURNAL OF BIOLOGICAL CHEMISTRY 2016
- 关键词：DNA-protein interaction protein domain signal transduction transcription coactivator transcription regulation AOX1 promoter Mit1 Pichia pastoris glycerol repression methanol induction
- 质疑
- CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris被引量：89收藏
- 作者：Liu, Qi Shi, Xiaona Song, Lili Liu, Haifeng Zhou, Xiangshan Wang, Qiyao Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Chinare Resources Angde Biotech Pharmaceut Co Ltd;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：MICROBIAL CELL FACTORIES 2019
- 关键词：Pichia pastoris CRISPR-Cas9 Homology directed repair Multiloci integration Multistep enzymatic pathway
- 质疑
- Zearalenone: A Mycotoxin With Different Toxic Effect in Domestic and Laboratory Animals' Granulosa Cells被引量：80收藏
- 作者：Zhang, Guo-Liang Feng, Yu-Long Song, Jun-Lin Zhou, Xiang-Shan
- 机构：Qingdao Agr Univ;Dong E E Jiao Co Ltd;East China Univ Sci & Technol
- 来源：FRONTIERS IN GENETICS 2018
- 关键词：zearalenone toxicity swine Equus asinus granulosa cells
- 质疑
- Donkey genomes provide new insights into domestication and selection for coat color被引量：71收藏
- 作者：Wang, Changfa Li, Haijing Guo, Yu Huang, Jinming Sun, Yan Min, Jiumeng Wang, Jinpeng Fang, Xiaodong Zhao, Zicheng Wang, Shuai Zhang, Yanlin Liu, Qingfeng Jiang, Qiang Wang, Xiuge Guo, Yijun Yang, Chunhong Wang, Yinchao Tian, Fang Zhuang, Guilong Fan, Yanna Gao, Qican Li, Yuhua Ju, Zhihua Li, Jianbin Li, Rongling Hou, Minghai Yang, Guiwen Liu, Guiqin Liu, Wenqiang Guo, Jiao Pan, Shanshan Fan, Guangyi Zhang, Wei Zhang, Ruitao Yu, Jie Zhang, Xinhao Yin, Qi Ji, Chuanliang Jin, Yuanchun Yue, Guidong Liu, Mei Xu, Jiake Liu, Shimin Jordana, Jordi Noce, Antonia Amills, Marcel Wu, Dong Dong Li, Shuaicheng Zhou, Xiangshan Zhong, Jifeng
- 机构：Shandong Acad Agr Sci;Liaocheng Univ;Dong EE Jiao Co Ltd;BGI Shenzhen;City Univ Hong Kong
- 来源：NATURE COMMUNICATIONS 2020
- 质疑
- 不同甲醇流加策略对重组毕赤酵母高密度发酵生产水蛭素的影响被引量：66收藏
- 作者：周祥山 范卫民 张元兴
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《生物工程学报》 2002
- 关键词：甲醇 流加策略 重组毕赤酵母 高密度发酵 水蛭素
- 摘要：考察了不同甲醇流加策略对毕赤酵母高密度发酵生产水蛭素的影响。溶氧控制法不能有效地防止甲醇的过量流加。气相色谱离线检测法虽然防止了甲醇流加过量 ,但甲醇浓度的波动较大。利用甲醇传感器在线检测控制甲醇的流加可维持较恒定的甲醇...
- 下载全文在线阅读质疑
- A novel methanol-free Pichia pastoris system for recombinant protein expression被引量：60收藏
- 作者：Shen, Wei Xue, Ying Liu, Yiqi Kong, Chuixing Wang, Xiaolong Huang, Mengmeng Cai, Menghao Zhou, Xiangshan Zhang, Yuanxing Zhou, Mian
- 机构：East China Univ Sci & Technol;SCICB
- 来源：MICROBIAL CELL FACTORIES 2016
- 关键词：Recombination protein expression AOX1 promoter Dihydroxyacetone GUT1 DAK Pichia pastoris
- 质疑
- An upstream activation sequence controls the expression of AOX1 gene in Pichia pastoris被引量：54收藏
- 作者：Xuan, Yaoji Zhou, Xiangshan Zhang, Wenwen Zhang, Xiao Song, Zhiwei Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Agcy Sci Technol & Res
- 来源：FEMS YEAST RESEARCH 2009
- 关键词：Pichia pastoris AOX1 promoter transcriptional regulation
- 质疑
- Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production被引量：52收藏
- 作者：Gao, Limei Cai, Menghao Shen, Wei Xiao, Siwei Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2013
- 关键词：Polyketide Pichia pastoris 6-MSA 6-MSAS Heterologous expression Fermentation
- 质疑
- Catabolite Repression of Aox in Pichia pastoris Is Dependent on Hexose Transporter PpHxt1 and Pexophagy被引量：52收藏
- 作者：Zhang, Ping Zhang, Wenwen Zhou, Xiangshan Bai, Peng Cregg, James M. Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Keck Grad Inst Appl Life Sci
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2010
- 质疑
- Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida被引量：51收藏
- 作者：Yin, Kaiyu Guan, Yunpeng Ma, Ruiqing Wei, Lifan Liu, Bing Liu, Xiaohong Zhou, Xiangshan Ma, Yue Zhang, Yuanxing Waldor, Matthew K. Wang, Qiyao
- 机构：East China Univ Sci & Technol;Qingdao Natl Lab Marine Sci & Technol;East China Univ Sci & Technol;Univ Autonoma Barcelona;Brigham & Womens Hosp
- 来源：PLOS PATHOGENS 2018
- 质疑
- Catabolite Repression of Aox in Pichia pastoris is dependent on hexose transporter PpHxt1 and pexophagy被引量：50收藏
- 作者：Zhang, Ping Zhang, Wenwen Zhou, Xiangshan Bai, Peng Cregg, James M. Zhang, Yuanxing
- 机构： Keck Graduate Institute of Applied Life Sciences; State Key Laboratory of Bioreactor Engineering
- 来源：Applied and Environmental Microbiology 2010
- 关键词：Glucose sensors - Glucose oxidase - Fructose - Gene expression - Yeast
- 质疑
- Decrease of proteolytic degradation of recombinant hirudin produced by Pichia pastoris by controlling the specific growth rate被引量：50收藏
- 作者：Zhou, XS Zhang, YX
- 机构：E China Univ Chem Technol
- 来源：BIOTECHNOLOGY LETTERS 2002
- 关键词：fermentation Pichia pastoris proteolytic degradation recombinant hirudin specific growth rate
- 质疑
- Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation被引量：45收藏
- 作者：Xiao, Anfeng Zhou, Xiangshan Zhou, Li Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2006
- 质疑
- Engineered ethanol-driven biosynthetic system for improving production of acetyl-CoA derived drugs in Crabtree-negative yeast被引量：45收藏
- 作者：Liu, Yiqi Bai, Chenxiao Liu, Qi Xu, Qin Qian, Zhilan Peng, Qiangqiang Yu, Jiahui Xu, Mingqiang Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：METABOLIC ENGINEERING 2019
- 关键词：Ethanol Crabtree-negative yeast Acetyl-CoA Monacolin J Metabolic engineering Coculture
- 质疑
- Characterization of a thermostable raw-starch hydrolyzing α-amylase from deep-sea thermophile Geobacillus sp.被引量：43收藏
- 作者：Jiang, Tao Cai, Menghao Huang, Mengmeng He, Hao Lu, Jian Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：PROTEIN EXPRESSION AND PURIFICATION 2015
- 关键词：alpha-Amylase Geobacillus sp. Heterologous expression Thermophile
- 质疑
- Regulating unfolded protein response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris被引量：39收藏
- 作者：Huang, Mengmeng Gao, Yanyun Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2017
- 关键词：Unfolded protein response HAC1p Heterologous expression P. pastoris Raw-starch hydrolyzing alpha-amylase
- 质疑
- PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris被引量：38收藏
- 作者：Wang, Xiaolong Cai, Menghao Shi, Lei Wang, Qi Zhu, Jinxiang Wang, Jinjia Zhou, Mian Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：BIOTECHNOLOGY LETTERS 2016
- 关键词：AOX1 promoter Catabolite repression Glucose Glycerol PpNrg1 Pichia pastoris Transcriptional regulation
- 质疑
- Improvement of cell viability and hirudin production by ascorbic acid in Pichia pastoris fermentation被引量：37收藏
- 作者：Xiao, Anfeng Zhou, Xiangshan Zhou, Li Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2006
- 关键词：Biotechnology - Cell membranes - Cells - Fermentation - Microbiology - Oxygen
- 质疑
- Genetically shaping morphology of the filamentous fungus Aspergillus glaucus for production of antitumor polyketide aspergiolide A被引量：37收藏
- 作者：Cai, Menghao Zhang, Ying Hu, Wei Shen, Wei Yu, Zhenzhong Zhou, Weiqiang Jiang, Tao Zhou, Xiangshan Zhang, Yuanxing
- 机构：E China Univ Sci & Technol
- 来源：MICROBIAL CELL FACTORIES 2014
- 关键词：Filamentous fungi Aspergillus glaucus Genetically morphology shaping Shear stress Aspergiolide A
- 质疑
- Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway被引量：36收藏
- 作者：Xue, Ying Kong, Chuixing Shen, Wei Bai, Chenxiao Ren, Yanna Zhou, Xiangshan Zhang, Yuanxing Cai, Menghao
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：JOURNAL OF BIOTECHNOLOGY 2017
- 关键词：Polyketides Pichia pastoris Heterologous biosynthesis Citrinin Biosynthetic pathway
- 质疑
- Isolation of a Virulent Aeromonas salmonicida subsp. masoucida Bacteriophage and Its Application in Phage Therapy in Turbot (Scophthalmus maximus)被引量：36收藏
- 作者：Xu, Zhenhe Jin, Peng Zhou, Xiangshan Zhang, Yuanxing Wang, Qiyao Liu, Xiaohong Shao, Shuai Liu, Qin
- 机构：East China Univ Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai;Shanghai Engn Res Ctr Maricultured Anim Vaccines
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2021
- 关键词：Aeromonas salmonicida subsp. masoucida antibacterial aquaculture phage therapy turbot Scophthalmus maximus
- 质疑
- Improving intracellular production of recombinant protein in Pichia pastoris using an optimized preinduction glycerol-feeding scheme被引量：36收藏
- 作者：Wei, Chun Zhou, Xiangshan Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Microbiology and Biotechnology 2008
- 关键词：Cells - Fermentation - Glycerol - Growth rate - Hormones - Proteins
- 质疑
- Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously被引量：35收藏
- 作者：Sun, Xueqian Zhou, Xiangshan Cai, Menghao Tao, Kejing Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2009
- 关键词：Ketones - Biosynthesis - Metabolites
- 质疑
- Significance of seed culture methods on mycelial morphology and production of a novel anti-cancer anthraquinone by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403)被引量：34收藏
- 作者：Yu, Chao Cai, Menghao Kang, Li Zhang, Yuanxing Zhou, Xiangshan
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2012
- 关键词：Mycelium - Aromatic compounds - Biomass - Bottles - Diseases - Ketones
- 质疑
- Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungus Aspergillus glaucus被引量：34收藏
- 作者：Sun, Xueqian Zhou, Xiangshan Cai, Menghao Zhou, Jiushun Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Bioresource Technology 2010
- 关键词：Carboxylic acids - Biosynthesis - Ketones - Biochemistry
- 质疑
- Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus被引量：32收藏
- 作者：Cai, Meng-Hao Zhou, Xiang-Shan Sun, Xue-Qian Tao, Ke-Jing Zhang, Yuan-Xing
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2009
- 关键词：Aspergillus glaucus Aspergiolide A Statistical methodologies Medium optimization Carbon-nitrogen ratio
- 质疑
- Zebrafish gasdermin E cleavage-engaged pyroptosis by inflammatory and apoptotic caspases被引量：32收藏
- 作者：Chen, Hao Wu, Xiaomin Gu, Zhaoyan Chen, Shouwen Zhou, Xiangshan Zhang, Yuanxing Liu, Qin Wang, Zhuang Yang, Dahai
- 机构：East China Univ Sci & Technol;Southern Marine Sci & Engn Guangdong Lab Zhuhai;Shanghai Engn Res Ctr Maricultured Anim Vaccines
- 来源：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 2021
- 关键词：Zebrafish Gsdme Pyroptosis Inflammatory caspases Apoptotic caspases
- 质疑
- Roles of cobalt in biosynthesis stimulation of a cytotoxic compound from marine-derived Aspergillus glaucus被引量：30收藏
- 作者：Cai, Menghao Sun, Xueqian Zhou, Xiangshan Zhang, Yuanxing
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2012
- 关键词：Aspergillus - Biochemistry - Biosynthesis - Cobalt - Ketones - Trace elements
- 质疑
