成果/Result
- A comparative assessment of cytotoxicity of commonly used agricultural insecticides to human and insect cells被引量：37收藏
- 作者：Yun, Xinming Huang, Qingchun Rao, Wenbing Xiao, Ciying Zhang, Tao Mao, Zhifan Wan, Ziyi
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2017
- 关键词：Insecticides Cytotoxicity Human cells Insect cells Cell-based systems
- Improved oxytetracycline production in Streptomyces rimosus M4018 by metabolic engineering of the G6PDH gene in the pentose phosphate pathway被引量：33收藏
- 作者：Tang, Zhenyu Xiao, Ciying Zhuang, Yingping Chu, Ju Zhang, Siliang Herron, Paul R. Hunter, Iain S. Guo, Meijin
- 机构：E China Univ Sci & Technol;Univ Strathclyde
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2011
- 关键词：Glucose-6-phosphate dehydrogenase Streptomyces rimosus M4018 Oxytetracycline biosynthesis NADPH generation Malonyl-CoA
- Emamectin benzoate induces ROS-mediated DNA damage and apoptosis in Trichoplusia Tn5B1-4 cells被引量：33收藏
- 作者：Luan, Shaorong Yun, Xinming Rao, Wenbing Xiao, Ciying Xu, Zhikang Lang, Jialin Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：CHEMICO-BIOLOGICAL INTERACTIONS 2017
- 关键词：Emamectin benzoate Cytotoxic action ROS DNA damage Cell apoptosis
- Improved oxytetracycline production in Streptomyces rimosus M4018 by metabolic engineering of the G6PDH gene in the pentose phosphate pathway被引量：31收藏
- 作者：Tang, Zhenyu Xiao, Ciying Zhuang, Yingping Chu, Ju Zhang, Siliang Herron, Paul R. Hunter, Iain S. Guo, Meijin
- 机构： State Key Laboratory of Bioreactor Engineering; Strathclyde Institute of Pharmacy and Biomedical Sciences
- 来源：Enzyme and Microbial Technology 2011
- 关键词：Biochemistry - Gene expression - Metabolic engineering - Carbon - Ketones - Bacteria - Biomolecules - Biosynthesis - Enzymes - Glucose - Physiology - Metabolites - Antibiotics
- Oxytetracycline biosynthesis improvement in Streptomyces rimosus following duplication of minimal PKS genes被引量：30收藏
- 作者：Yu, Lan Cao, Nan Wang, Long Xiao, Ciying Guo, Meijin Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构： State Key Laboratory of Bioreactor Engineering; School of Life and Science
- 来源：Enzyme and Microbial Technology 2012
- 关键词：Bacteria - Growth kinetics - Ketones - Cell growth - Plants (botany) - Polymerase chain reaction - Biochemistry - Morphology - Transcription
- Apoptosis of leukemia K562 and Molt-4 cells induced by emamectin benzoate involving mitochondrial membrane potential loss and intracellular Ca2+ modulation被引量：22收藏
- 作者：Yun, Xinming Rao, Wenbing Xiao, Ciying Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 2017
- 关键词：Cell apoptosis K562 Molt-4 Emamectin benzoate MMP loss [Ca2+](i) modulation
- Antioxidative cellular response of lepidopteran ovarian cells to photoactivated alpha-terthienyl被引量：21收藏
- 作者：Huang, Qingchun Yun, Xinming Rao, Wenbing Xiao, Ciying
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 2017
- 关键词：Alpha-terthienyl Tn5B1-4 cells Sf-21 cells Antioxidative response Cytotoxicity
- Fungicidal activity of novel quinazolin-6-ylcarboxylates and mode of action on Botrytis cinerea被引量：18收藏
- 作者：Xu, Jialin Yan, Dongmei Chen, Yongjun Cai, Danni Huang, Fengcheng Zhu, Lisong Zhang, Xianfei Luan, Shaorong Xiao, Ciying Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：PEST MANAGEMENT SCIENCE 2023
- 关键词：quinazolin-6-ylcarboxylates fungicidal activity suppressive efficacy mode of action Botrytis cinerea
- Oxytetracycline biosynthesis improvement in Streptomyces rimosus following duplication of minimal PKS genes被引量：15收藏
- 作者：Yu, Lan Cao, Nan Wang, Long Xiao, Ciying Guo, Meijin Chu, Ju Zhuang, Yingping Zhang, Siliang
- 机构：E China Univ Sci & Technol;Huzhou Teachers Coll
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2012
- 关键词：Minimal PKS genes Overexpression Morphology Oxytetracycline
- The effect of α-tocopherol and dithiothreitol in ameliorating emamectin benzoate cytotoxicity in human K562 cells involving the modulation of ROS accumulation and NF-κB signaling被引量：12收藏
- 作者：Luan, Shaorong Muhayimana, Solange Xu, Jiuyong Zhang, Xianfei Xiao, Ciying Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 2019
- 关键词：EMB cytotoxicity alpha-tocopherol Dithiothreitol Oxidative stress NF-kappa B signaling Human K562 cells
- The importance of selecting crystal form for triazole fungicide tebuconazole to enhance its botryticidal activity被引量：11收藏
- 作者：Chen, Yongjun Yan, Dongmei Xu, Jialin Xiong, Hui Luan, Shaorong Xiao, Ciying Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：SCIENCE OF THE TOTAL ENVIRONMENT 2023
- 关键词：Tebuconazole Crystal form Fungicidal activity TCA metabolism Point mutation Botrytis cinerea
- Novel Galactosyl Moiety-Conjugated Furylchalcones Synthesized Facilely Display Significant Regulatory Effect on Plant Growth被引量：9收藏
- 作者：Liu, Xuefeng Chen, Yongjun Deng, Yunfei Xiao, Ciying Luan, Shaorong Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2022
- 关键词：galactosyl furylchalcones plant growth herbicidal activity syntheses molecular docking
- 必特螺旋霉素基因工程菌发酵参数的研究被引量：9收藏
- 作者：戴剑漉 王永红 刘玉伟 肖慈英 佟祖佲 储炬 张嗣良 律光耀 王以光
- 机构：中国医学科学院中国协和医科大学医药生物技术研究所;华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心;北京赛而生物药业有限公司
- 来源：《中国抗生素杂志》 2005
- 关键词：必特螺旋霉索 溶氧 参数检测
- 摘要：曾利用同源重组技术构建了稳定的必特螺旋霉素基因工程菌。本研究在30L全自动发酵罐从菌体生长,糖、氮源利用及发酵效价增长情况考察了必特螺旋霉素基因工程菌的发酵过程,研究了发酵过程摄氧率(OUR)、二氧化碳释放率(CER)、...
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- Photosensitization of Chinese hamster V79 cells to photoactivated alpha-terthienyl involving membrane damage and oxidative stress被引量：8收藏
- 作者：Luan, Shaorong Muhayimana, Solange Xu, Jiuyong Zhang, Xianfei Xiao, Ciying Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY 2018
- 关键词：Chinese hamster V79 cells Alpha-terthienyl Membrane damage Oxidative stress Photoactivated cytotoxic effect
- Tolerance potential of Chilo suppressalis larvae to fipronil exposure via the modulation of detoxification and GABA responses被引量：7收藏
- 作者：Xiao, Ciying Luan, Shaorong Xu, Zhikang Lang, Jialin Rao, Wenbing Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY 2017
- 关键词：Chilo suppressalis Fipronil Detoxification enzymes Glutamate Gamma-aminobutyric acid
- 醉金香葡萄愈伤细胞悬浮培养基优化促进白藜芦醇的合成被引量：5收藏
- 作者：王晓惠 王泽建 肖慈英 刘泽波 郭美锦 庄英萍
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2022
- 关键词：醉金香葡萄 愈伤细胞 悬浮培养 营养组分优化 白藜芦醇
- 摘要：建立和优化了一种醉金香葡萄悬浮细胞合成白藜芦醇的工艺。以细胞生长和白藜芦醇生物合成量为响应值,通过单因素实验、Plackett-Burman、响应面实验考察了不同碳源、氮源、前体、诱导剂和激素等因素对醉金香葡萄细胞悬浮培...
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- Exposure to emamectin benzoate confers cytotoxic effects on human molt-4 T-cells and possible ameliorative role of vitamin E and dithiothreitol被引量：5收藏
- 作者：Chen, Yongjun Liu, Xuefeng Yan, Dongmei Xu, Jialin Luan, Shaorong Xiao, Ciying Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：DRUG AND CHEMICAL TOXICOLOGY 2023
- 关键词：Emamectin benzoate lymphocyte toxicity oxidative stress NF-kappa B signaling pathway Molt-4 T-cells
- Purification and biochemical characterization of a novel transglutaminase from Mythimna separata larvae (Noctuidae, Lepidoptera)被引量：4收藏
- 作者：Zhang, Lei Rao, Wenbing Muhayimana, Solange Zhang, Xianfei Xu, Jiuyong Xiao, Ciying Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2018
- 关键词：Larval transglutaminase Mythimna separata Purification Biochemical property
- 龟裂链霉菌工业菌中zwf1基因的阻断对土霉素生物合成影响被引量：3收藏
- 作者：郑子静 于岚 唐振宇 郭元昕 肖慈英 郭美锦
- 机构：华东理工大学生物反应器工程国家重点实验室国家生化工程技术研究中心(上海)
- 来源：《中国抗生素杂志》 2012
- 关键词：龟裂链霉菌工业菌 葡萄糖-6-磷酸脱氢酶 基因阻断 土霉素生物合成
- 摘要：目的运用代谢工程手段对龟裂链霉菌工业菌(Industrial Streptomyces rimosus,SRI)进行基因改造,提高土霉素(oxytetracycline,OTC)产量。方法利用pKC1139质粒阻断SRI...
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- 低氧诱导启动子PsADH2在毕赤酵母中的调控特性被引量：2收藏
- 作者：乔雪锋 陆俊杰 肖慈英 储炬 钱江潮
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2013
- 关键词：PsADH2启动子 低氧诱导 透明颤藻血红蛋白 毕赤酵母
- 摘要：树干毕赤酵母(Pichia stipitis)乙醇脱氢酶Ⅱ(Alcohol dehydrogenaseⅡ)基因PsADH2的启动子是低氧诱导型启动子。利用透明颤藻血红蛋白(VHb)基因vgb、绿色荧光蛋白(GFP)基因g...
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- 甘蔗糖蜜促进类球红细菌辅酶Q_(10)的糖转化效率被引量：2收藏
- 作者：葛燕 栗波 刘爱军 肖慈英 陈必钦 朱志春 梁剑光 庄英萍 王泽建
- 机构：常州大学药学院;华东理工大学生物反应器工程国家重点实验室;河北欣港药业有限公司;内蒙古金达威药业有限公司
- 来源：《华东理工大学学报（自然科学版）》 2024
- 关键词：类球红细菌 甘蔗糖蜜 辅酶Q_(10) 发酵优化 糖转化效率
- 摘要：考察了甘蔗糖蜜对类球红细菌发酵生产辅酶Q_(10)生物合成的影响,优化得到最佳的甘蔗糖蜜添加工艺,提升了糖转化率,大幅度降低了生产成本。不同碳源底物对辅酶Q_(10)产物合成有较大的影响,其中葡萄糖为最适碳源,利于类球红...
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- Novel quinazolin-6-yl Isoindolinone: Altering polysaccharide chemstructure for antibacterial efficacy against Staphylococcus aureus被引量：2收藏
- 作者：Huang, Qingchun Zhu, Lisong Huang, Fengcheng Zhao, Yanjun Wang, Hongye Luan, Shaorong Xiao, Ciying
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2024
- 关键词：Quinazolin-6-yl isoindolinone Antibacterial efficacy Biofilm formation Polysaccharide modulation N -acetylaminoglucosidase Staphylococcus aureus
- 内源信号肽DSE4介导头孢菌素C酰化酶在毕赤酵母中的分泌表达被引量：2收藏
- 作者：丁璐妹 肖慈英 储炬 钱江潮 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《华东理工大学学报（自然科学版）》 2015
- 关键词：毕赤酵母 分泌表达 DSE4信号肽 头孢菌素C酰化酶 β-半乳糖苷酶
- 摘要：头孢菌素C(CPC)酰化酶可用于一步酶法合成7-氨基头孢烷酸(7-ACA)。对来自Pseudomonas sp.Strain SE83的高活性CPC酰化酶突变基因进行基于毕赤酵母偏好密码子优化,获得基因SECA。选取毕赤...
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- Revealing TCA Cycle Flow Dynamics in Fungicide-Adapted Botrytis cinerea through Anionic Chromatographic Simultaneous Separation被引量：1收藏
- 作者：Zhao, Yanjun Zhao, Sijia Wang, Xiaowei Luan, Shaorong Xiao, Ciying Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2025
- 关键词：TCA cycle flow simultaneous separation fungicideadaptation
Botrytis cinerea
- 基于易错PCR技术的红酵母D-氨基酸氧化酶的定向进化被引量：1收藏
- 作者：孙晨 肖慈英 郭美锦 储炬 张嗣良
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：《工业微生物》 2014
- 关键词：易错PCR 定向进化 高通量筛选 红酵母D-氨基酸氧化酶
- 摘要：采用易错PCR技术对来源于红酵母Rhodotorula gracilis的D-氨基酸氧化酶基因(RgDAAO)进行突变,构建并优化了突变株文库;结合48深孔板的高通量筛选方法,获得突变株M3217,其V_(max)相对于...
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- Antifungal Activity of Novel Isoindoline-2-Yl Putrescines as Potential Autophagy-Activated Fungicide被引量：1收藏
- 作者：Zhu, Lisong Huang, Fengcheng Wang, Hongye Zhao, Yanjun Luan, Shaorong Xiao, Ciying Huang, Qingchun
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：CHEMISTRY & BIODIVERSITY 2025
- 关键词：Isoindoline-2-yl putrescine
In vivo antifungal activity Molecular docking Plant fungal pathogen
- 低氧诱导启动子PsADH2在毕赤酵母中的调控特性被引量：0收藏
- 作者：乔雪锋 陆俊杰 肖慈英 储炬 钱江潮
- 机构：华东理工大学生物反应器工程国家重点实验室
- 来源：[1]华东理工大学生物反应器工程国家重点实验室; 0
- 关键词：PsADH2启动子 低氧诱导 透明颤藻血红蛋白 毕赤酵母
- 巨大芽孢杆菌发酵合成有机酸的条件优化被引量：0收藏
- 作者：王心语 余君雄 张子恕 肖慈英 张红兵 王泽建
- 机构：河北经贸大学生物科学与工程学院;华东理工大学生物工程学院
- 来源：《河北科技大学学报》 2026
- 关键词：发酵工程 巨大芽孢杆菌 有机酸 工艺优化 过程控制
- 摘要：为提高巨大芽孢杆菌(Bacillus megaterium)补料分批发酵体系的有机酸产量与底物转化率,采用摇瓶与5 L生物反应器,对培养基组分和发酵过程控制工艺条件进行优化,并建立在线耗氧速率(oxygen uptake...
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- Electronic Nose-Based Methanol Feedback Precision Control Strategy for High-Yield Alkaline Phosphatase Fermentation by Hansenula polymorpha被引量：0收藏
- 作者：Liang, Jian-Guang Wang, Shao-Jie Wang, Jia-Jia Siddique, Muhammad Safwan Zhang, Zishu Xiao, Ciying Wang, Ze-Jian
- 机构：Changzhou Univ;Changzhou Univ;Hebei Univ Econ & Business;East China Univ Sci & Technol
- 来源：FOOD BIOENGINEERING 2026
- 关键词：alkaline phosphatase animal food nutrient feedback control precision control strategy
- 龟裂链霉菌中选择性σ因子lsigB在大肠杆菌中的异源表达及功能验证被引量：0收藏
- 作者：曹楠 郭美锦 肖慈英
- 机构：华东理工大学生物工程学院
- 来源：《食品工业科技》 2015
- 关键词：龟裂链霉菌 sigma因子 大肠杆菌
- 摘要：本文扩增了龟裂链霉菌中选择性调控因子的编码基因lsig B,并在大肠杆菌中进行异源表达。通过SDS-PAGE以及Western blotting方法鉴定Lsig B蛋白后,在多种环境压力下培养重组大肠杆菌。实验结果表明,...
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