成果/Result
- Biocatalytic ketone reduction: A green and efficient access to enantiopure alcohols被引量：200收藏
- 作者：Ni, Yan Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY ADVANCES 2012
- 关键词：Asymmetric reduction Biocatalysis Chiral alcohols Genome mining Reductases
- Biocatalysis in development of green pharmaceutical processes被引量：190收藏
- 作者：Tao, Junhua Xu, Jian-He
- 机构：Elevance Renewable Sci Inc;E China Univ Sci & Technol
- 来源：CURRENT OPINION IN CHEMICAL BIOLOGY 2009
- Nano-SiO2 induces apoptosis via activation of p53 and Bax mediated by oxidative stress in human hepatic cell line被引量：170收藏
- 作者：Ye, Yiyi Liu, Jianwen Xu, Jianhe Sun, Lijuan Chen, Mingcang Lan, Minbo
- 机构：E China Univ Sci & Technol
- 来源：TOXICOLOGY IN VITRO 2010
- 关键词：Nano-SiO2 Oxidative stress Apoptosis p53 Bax Human hepatic cells
- Cloning and characterization of a panel of constitutive promoters for applications in pathway engineering in Saccharomyces cerevisiae被引量：149收藏
- 作者：Sun, Jie Shao, Zengyi Zhao, Hua Nair, Nikhil Wen, Fei Xu, Jian-He Zhao, Huimin
- 机构：Univ Illinois;E China Univ Sci & Technol;Univ Illinois
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2012
- 关键词：yeast promoters xylanases zeaxanthin consolidated bioprocessing synthetic biology
- New opportunities for biocatalysis: driving the synthesis of chiral chemicals被引量：148收藏
- 作者：Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CURRENT OPINION IN BIOTECHNOLOGY 2011
- 关键词：Stereochemistry - Biocatalysts - Biochemical engineering
- Immobilization of Serratia marcescens lipase onto amino-functionalized magnetic nanoparticles for repeated use in enzymatic synthesis of Diltiazem intermediate被引量：140收藏
- 作者：Hu, Bin Pan, Jiang Yu, Hui-Lei Liu, Jian-Wen Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：PROCESS BIOCHEMISTRY 2009
- 关键词：Lipase Immobilization Magnetic nanoparticles Enzymatic biocatalytic resolution Diltiazem intermediate Biphasic reaction
- Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor被引量：129收藏
- 作者：Wang, Li-Juan Li, Chun-Xiu Ni, Yan Zhang, Jie Liu, Xiang Xu, Jian-He
- 机构：E China Univ Sci & Technol;Jiangnan Univ
- 来源：BIORESOURCE TECHNOLOGY 2011
- 关键词：Carbonyl reductase Streptomyces coelicolor Asymmetric reduction Ethyl (S)-4-chloro-3-hydroxybutanoate NADH regeneration
- Enhancing effect of Tween-80 on lipase performance in enantioselective hydrolysis of ketoprofen ester被引量：113收藏
- 作者：Liu, YY Xu, JH Hu, Y
- 机构：E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2000
- 关键词：Candida rugosa lipase enantioselectivity activity ketoprofen Tween-80
- Asymmetric Amination of Secondary Alcohols by using a Redox-Neutral Two-Enzyme Cascade被引量：112收藏
- 作者：Chen, Fei-Fei Liu, You-Yan Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol;Guangxi Univ;Guangxi Acad Sci
- 来源：CHEMCATCHEM 2015
- 关键词：amine dehydrogenase asymmetric catalysis biotransformations cascade reaction chiral amines
- Reshaping the Active Pocket of Amine Dehydrogenases for Asymmetric Synthesis of Bulky Aliphatic Amines被引量：102收藏
- 作者：Chen, Fei-Fei Zheng, Gao-Wei Liu, Lei Li, Hao Chen, Qi Li, Fu-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2018
- 关键词：amine dehydrogenase reductive amination chiral amine protein engineering biocatalysis substrate scope
- Development of an Engineered Ketoreductase with Simultaneously Improved Thermostability and Activity for Making a Bulky Atorvastatin Precursor被引量：101收藏
- 作者：Gong, Xu-Min Qin, Zhen Li, Fu-Long Zeng, Bu-Bing Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis asymmetric reduction ketoreductase protein engineering thermostabilization atorvastatin precursor
- Reshaping an Enzyme Binding Pocket for Enhanced and Inverted Stereoselectivity: Use of Smallest Amino Acid Alphabets in Directed Evolution被引量：98收藏
- 作者：Sun, Zhoutong Lonsdale, Richard Kong, Xu-Dong Xu, Jian-He Zhou, Jiahai Reetz, Manfred T.
- 机构：Max Planck Inst Kohlenforsch;Univ Marburg;E China Univ Sci & Technol;Chinese Acad Sci
- 来源：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2015
- 关键词：amino acid alphabet directed evolution epoxide hydrolases saturation mutagenesis stereoselectivity
- Biochemical properties and potential applications of an organic solvent-tolerant lipase isolated from Serratia marcescens ECU1010被引量：84收藏
- 作者：Zhao, Li-Li Xu, Jian-He Zhao, Jian Pan, Jiang Wang, Zhi-Long
- 机构：E China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：PROCESS BIOCHEMISTRY 2008
- 关键词：lipase Serratia marcescens organic solvent tolerance enantiomeric resolution chiral compounds biochemical properties
- Whole cell microbial transformation in cloud point system被引量：84收藏
- 作者：Wang, Zhilong Xu, Jian-He Chen, Daijie
- 机构： School of Pharmacy; Laboratory of Biocatalysis and Bioprocessing; Shanghai Institutes of Pharmacy Industry
- 来源：Journal of Industrial Microbiology and Biotechnology 2008
- 关键词：Biocompatibility - Nonionic surfactants - Phase separation - Solvent extraction
- Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp ECU0401被引量：83收藏
- 作者：He, Yu-Cai Xu, Jian-He Su, Jin-Huan Zhou, Li
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Nitrilase Alcaligenes sp ECU0401 Glycolic acid Glycolonitrile Immobilization
- Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1被引量：82收藏
- 作者：Zheng, Gao-Wei Liu, Yuan-Yang Chen, Qi Huang, Lei Yu, Hui-Lei Lou, Wen-Yong Li, Chun-Xiu Bai, Yun-Peng Li, Ai-Tao Xu, Jian-He
- 机构：East China Univ Sci & Technol;South China Univ Technol;Max Planck Inst Kohlenforsch
- 来源：ACS CATALYSIS 2017
- 关键词：asymmetric reduction ketoreductase chiral alcohol protein engineering substrate specificity biocatalysis
- Engineering of an epoxide hydrolase for efficient bioresolution of bulky pharmaco substrates被引量：81收藏
- 作者：Kong, Xu-Dong Yuan, Shuguang Li, Lin Chen, She Xu, Jian-He Zhou, Jiahai
- 机构：E China Univ Sci & Technol;Chinese Acad Sci;Natl Inst Biol Sci
- 来源：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 2014
- 关键词：epoxide hydrolase X-ray crystallography protein engineering product release bulky substrate
- Newly Identified Thermostable Esterase from Sulfobacillus acidophilus: Properties and Performance in Phthalate Ester Degradation被引量：77收藏
- 作者：Zhang, Xiao-Yan Fan, Xiang Qiu, Yong-Jun Li, Cheng-Yuan Xing, Shuai Zheng, Yi-Tao Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2014
- 关键词：Escherichia coli - Esters - Hydrolysis
- A Motif-Based Deep Learning Tool for the Identification of Unusual NADH-Dependent Imine Reductases被引量：73收藏
- 作者：Shen, Xin-Yuan Wu, Yu-Xuan Ma, Zhi-Feng Yi, Xiao Shi, Min Zhu, Zhen-Yu Jin, Tian Hu, Xiao-Yu Huang, Zi-Yi Gao, Yun-Fei Chen, Qi Yu, Hui-Qun Xu, Jian-He Fan, Gui-Sheng Zheng, Gao-Wei
- 机构： State Key Laboratory of Bioreactor Engineering; School of Information Science and Engineering
- 来源：SSRN 2025
- 关键词：Amination - Enzyme immobilization - Enzyme inhibition - Enzyme kinetics - Hydrolases - Lipases
- Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution被引量：72收藏
- 作者：Li, Aitao Ilie, Adriana Sun, Zhoutong Lonsdale, Richard Xu, Jian-He Reetz, Manfred T.
- 机构：Max Planck Inst Kohlenforsch;Univ Marburg;East China Univ Sci & Technol
- 来源：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 2016
- 关键词：cascade reactions C-H activation directed evolution enantioselectivity P450 monooxygenase
- Access to Optically Active Aryl Halohydrins Using a Substrate-Tolerant Carbonyl Reductase Discovered from Kluyveromyces thermotolerans被引量：71收藏
- 作者：Xu, Guo-Chao Yu, Hui-Lei Zhang, Xiao-Yan Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ACS CATALYSIS 2012
- 关键词：carbonyl reductase asymmetric hydrogen transfer aryl halohydrin Kluyveromyces thermotolerans genome data mining substrate/product tolerance
- Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity被引量：68收藏
- 作者：Ni, Yan Li, Chun-Xiu Ma, Hong-Min Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：YtbE Recombinant aldo-keto reductase Bacillus sp. Asymmetric reduction Chiral alcohols
- Enantioselective Synthesis of Chiral Vicinal Amino Alcohols Using Amine Dehydrogenases被引量：67收藏
- 作者：Chen, Fei-Fei Cosgrove, Sebastian C. Birmingham, William R. Mangas-Sanchez, Juan Citoler, Joan Thompson, Matthew P. Zheng, Gao-Wei Xu, Jian-He Turner, Nicholas J.
- 机构：East China Univ Sci & Technol;Univ Manchester;EnginZyme AB
- 来源：ACS CATALYSIS 2019
- 关键词：amine dehydrogenase biocatalysis chiral amino alcohols directed evolution reductive amination
- Chemical modulators for enzymatic reactions被引量：66收藏
- 作者：Xu, Jian-He Zheng, Gao-Wei Luo, Xiao-Jing
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：RSC Green Chemistry 2015
- Switching Cofactor Dependence of 7β-Hydroxysteroid Dehydrogenase for Cost-Effective Production of Ursodeoxycholic Acid被引量：66收藏
- 作者：You, Zhi-Neng Chen, Qi Shi, Shou-Cheng Zheng, Ming-Min Pan, Jiang Qian, Xiao-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis cofactor dependence small-and-smart library 7 beta-hydroxysteroid dehydrogenase molecular dynamics simulation ursodeoxycholic acid
- Efficient Synthesis of Chiral Indolines using an Imine Reductase from Paenibacillus lactis被引量：66收藏
- 作者：Li, Hao Luan, Zheng-Jiao Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ADVANCED SYNTHESIS & CATALYSIS 2015
- 关键词：asymmetric hydrogenation biocatalysis chiral indolines imine reductase 3H-indoles
- Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques被引量：66收藏
- 作者：Zheng, Ming-Min Wang, Ru-Feng Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;China Pharmaceut Univ
- 来源：PROCESS BIOCHEMISTRY 2015
- 关键词：Ursodeoxycholic acid Chenodeoxycholic acid 7 alpha-Hydroxysteroid dehydrogenase 7 beta-Hydroxysteroid dehydrogenase Two-step reaction
- Bio-resolution of a chiral epoxide using whole cells of Bacillus megaterium ECU1001 in a biphasic system被引量：65收藏
- 作者：Gong, Peng-Fei Xu, Jian-He
- 机构： Lab. Biocatalysis and Bioprocessing
- 来源：Enzyme and Microbial Technology 2005
- 关键词：Biocatalysts - Cells - Concentration (process) - Ethers - Optimization - Solubility
- Direct Conversion of Xylan to Ethanol by Recombinant Saccharomyces cerevisiae Strains Displaying an Engineered Minihemicellulosome被引量：63收藏
- 作者：Sun, Jie Wen, Fei Si, Tong Xu, Jian-He Zhao, Huimin
- 机构：Univ Illinois;E China Univ Sci & Technol; Univ Illinois
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2012
- Bio-resolution of a chiral epoxide using whole cells of Bacillus megaterium ECU1001 in a biphasic system被引量：63收藏
- 作者：Gong, PF Xu, JH
- 机构：E China Univ Sci & Technol
- 来源：ENZYME AND MICROBIAL TECHNOLOGY 2005
- 关键词：kinetic resolution glycidyl phenyl ether epoxide hydrolase Bacillus megaterium enantiospecific hydrolysis organic-aqueous two-liquid system stirred reactor
