成果/Result
- Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1被引量：82收藏
- 作者：Zheng, Gao-Wei Liu, Yuan-Yang Chen, Qi Huang, Lei Yu, Hui-Lei Lou, Wen-Yong Li, Chun-Xiu Bai, Yun-Peng Li, Ai-Tao Xu, Jian-He
- 机构：East China Univ Sci & Technol;South China Univ Technol;Max Planck Inst Kohlenforsch
- 来源：ACS CATALYSIS 2017
- 关键词：asymmetric reduction ketoreductase chiral alcohol protEIn engineering substrate specificity biocatalysis
- An engineered Pseudomonas putida can simultaneously degrade organophosphates, pyrethroids and carbamates被引量：64收藏
- 作者：Gong, Ting Xu, Xiaoqing Dang, Yulei Kong, Annie Wu, Yunbo Liang, Peixin Wang, Shufang Yu, Huilei Xu, Ping Yang, Chao
- 机构：Nankai Univ;Nankai Univ;East China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：SCIENCE OF THE TOTAL ENVIRONMENT 2018
- 关键词：Pesticide Bioremediation Metabolic engineering Pseudomonas putida KT2440
- Engineering of Cyclohexanone Monooxygenase for the Enantioselective Synthesis of (S)-Omeprazole被引量：59收藏
- 作者：Zhang, Yan Wu, Yin-Qi Xu, Na Zhao, Qian Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangsu Aosaikang Pharmaceut Co Ltd
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2019
- 关键词：Biocatalysis Cyclohexanone monooxygenase Substrate specificity ProtEIn engineering Esomeprazole sulfoxide Halo-based selection method
- Access to optically active aryl halohydrins using a substrate-tolerant carbonyl reductase discovered from kluyveromyces thermotolerans被引量：59收藏
- 作者：Xu, Guo-Chao Yu, Hui-Lei Zhang, Xiao-Yan Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：ACS Catalysis 2012
- 关键词：Data mining - Escherichia coli - Enzyme activity - Genes - Ketones
- A thermostable and organic-solvent tolerant esterase from Pseudomonas putida ECU1011: Catalytic properties and performance in kinetic resolution of α-hydroxy acids被引量：49收藏
- 作者：Ma, Bao-Di Yu, Hui-Lei Pan, Jiang Liu, Jia-Yan Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2013
- 关键词：Esterase Thermostability Organic-solvent tolerance alpha-Hydroxy (carboxylic) acids Deacetylation
- Hydroxylases involved in terpenoid biosynthesis: a review被引量：47收藏
- 作者：Zhang, Zihan Wu, Qing-Yang Ge, Yue Huang, Zheng-Yu Hong, Ran Li, Aitao Xu, Jian-He Yu, Hui-Lei
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Hubei Univ
- 来源：BIORESOURCES AND BIOPROCESSING 2023
- 关键词：Biooxidation Cytochrome P450 monooxygenase Hydroxylase Natural products Terpenoids
- Enzymatic Preparation of the Chiral (S)-Sulfoxide Drug Esomeprazole at Pilot-Scale Levels被引量：45收藏
- 作者：Xu, Na Zhu, Jun Wu, Yin-Qi Zhang, Yan Xia, Jian-Ye Zhao, Qian Lin, Guo-Qiang Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangsu Aosaikang Pharmaceut Co Ltd
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2020
- 关键词：prazole sulfide monooxygenase esomeprazole asymmetric oxidation oxygen mass transfer reaction regulation
- Regioselectivity Engineering of Epoxide Hydrolase: Near-Perfect Enantioconvergence through a Single Site Mutation被引量：41收藏
- 作者：Li, Fu-Long Kong, Xu-Dong Chen, Qi Zheng, Yu-Cong Xu, Qin Chen, Fei-Fei Fan, Li-Qiang Lin, Guo-Qiang Zhou, Jiahai Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ
- 来源：ACS CATALYSIS 2018
- 关键词：biocatalysis epoxide hydrolase crystal structure regioselectivity enantioconvergence protEIn engineering
- Confining Enzyme Clusters in Bacteriophage P22 Enhances Cofactor Recycling and Stereoselectivity for Chiral Alcohol Synthesis被引量：40收藏
- 作者：Zhang, Yan-Qing Feng, Tao-Tao Cao, Yu-Fei Zhang, Xiao-Yan Wang, Tao Nina, Mario Roque Huanca Wang, Li-Cheng Yu, Hui-Lei Xu, Jian-He Ge, Jun Bai, Yun-Peng
- 机构：East China Univ Sci & Technol ECUST;Tsinghua Univ;Tsinghua Shenzhen Int Grad Sch;Shenzhen Bay Lab;Tsinghua Univ
- 来源：ACS CATALYSIS 2021
- 关键词：enzyme cluster enzymatic catalysis cofactor recycling confinement effects asymmetric reduction
- Increased Catalyst Productivity in α-Hydroxy Acids Resolution by Esterase Mutation and Substrate Modification被引量：39收藏
- 作者：Ma, Bao-Di Kong, Xu-Dong Yu, Hui-Lei Zhang, Zhi-Jun Dou, Shuai Xu, Yan-Peng Ni, Yan Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ACS CATALYSIS 2014
- 关键词：catalyst productivity deacylation enzymatic resolution alpha-hydroxy acid product:catalyst ratio semirational design
- Synthesis of optically pure S-sulfoxide by Escherichia coli transformant cells coexpressing the P450 monooxygenase and glucose dehydrogenase genes被引量：38收藏
- 作者：Zhang, Jian-Dong Li, Ai-Tao Yu, Hui-Lei Imanaka, Tadayuki Xu, Jian-He
- 机构：E China Univ Sci & Technol;Ritsumeikan Univ
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2011
- 关键词：Coexpression P450SMO Glucose dehydrogenase NADPH regeneration D-glucose Sulfoxidation
- Bioproduction of chiral mandelate by enantioselective deacylation of α-acetoxyphenylacetic acid using whole cells of newly isolated Pseudomonas sp ECU1011被引量：38收藏
- 作者：Ju, Xin Yu, Hui-Lei Pan, Jiang Wei, Dong-Zhi Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2010
- 关键词：Microbial esterase Pseudomonas sp. Enantioselective deacylation alpha-Acetoxyphenylacetic acid Enantiopure mandelic acid
- Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. Coli被引量：38收藏
- 作者：Du, Fu-Liang Yu, Hui-Lei Xu, Jian-He Li, Chun-Xiu
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Biochemistry - Biosynthesis - Flavor compounds - Gene expression - Monoterpenes
- Engineering Streptomyces coelicolor Carbonyl Reductase for Efficient Atorvastatin Precursor Synthesis被引量：36收藏
- 作者：Li, Min Zhang, Zhi-Jun Kong, Xu-Dong Yu, Hui-Lei Zhou, Jiahai Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2017
- 关键词：carbonyl reductase protEIn engineering catalytic activity thermostability ethyl-(S)-4-chloro-3-hydroxybutyrate
- Immobilization of glycolate oxidase from Medicago falcata on magnetic nanoparticles for application in biosynthesis of glyoxylic acid被引量：34收藏
- 作者：Zhu, Hong Pan, Jiang Hu, Bin Yu, Hui-Lei Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2009
- 关键词：Nanomagnetics - Nanoparticles - Fourier transform infrared spectroscopy - Batch reactors - Enzyme immobilization - Enzyme activity - Synthesis (chemical) - Biochemistry - Plants (botany) - Scanning electron microscopy
- Discovery of Two Native Baeyer-Villiger Monooxygenases for Asymmetric Synthesis of Bulky Chiral Sulfoxides被引量：33收藏
- 作者：Zhang, Yan Liu, Feng Xu, Na Wu, Yin-Qi Zheng, Yu-Cong Zhao, Qian Lin, Guoqiang Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangsu Aosaikang Pharmaceut Co Ltd;Chinese Acad Sci
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2018
- 关键词：Baeyer-Villiger monooxygenase ethionamide monooxygenase stereoselectivity asymmetric sulfoxidation prazole thioether
- Dehydrogenase-Catalyzed Oxidation of Furanics: Exploitation of Hemoglobin Catalytic Promiscuity被引量：33收藏
- 作者：Jia, Hao-Yu Zong, Min-Hua Yu, Hui-Lei Li, Ning
- 机构：South China Univ Technol;East China Univ Sci & Technol
- 来源：CHEMSUSCHEM 2017
- 关键词：cofactor regeneration enzyme catalysis enzyme promiscuity oxidation platform chemicals
- A Baeyer-Villiger monooxygenase from Cupriavidus basilensis catalyzes asymmetric synthesis of (R)-lansoprazole and other pharmaco-sulfoxides被引量：33收藏
- 作者：Liu, Feng Shou, Chao Geng, Qiang Zhao, Chen Xu, Jianhe Yu, Huilei
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2021
- 关键词：Baeyer-Villiger monooxygenase asymmetric sulfoxidation (R)-lansoprazole proton pump inhibitor
- Resolution of racemic sulfoxides with high productivity and enantioselectivity by a Rhodococcus sp. strain as an alternative to biooxidation of prochiral sulfides for efficient production of enantiopure sulfoxides被引量：32收藏
- 作者：Li, Ai-Tao Yu, Hui-Lei Pan, Jiang Zhang, Jian-Dong Xu, Jian-He Lin, Guo-Qiang
- 机构： Laboratory of Biocatalysis and Bioprocessing; Shanghai Institute of Organic Chemistry
- 来源：Bioresource Technology 2011
- 关键词：Substrates - Enantioselectivity
- Enantioselective bioreductive preparation of chiral halohydrins employing two newly identified stereocomplementary reductases被引量：32收藏
- 作者：Xu, Guo-Chao Yu, Hui-Lei Shang, Yue-Peng Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol;Jiangnan Univ
- 来源：RSC ADVANCES 2015
- 关键词：Enzyme activity - Ketones
- Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata被引量：32收藏
- 作者：Zhang, Chen-Sheng Zhang, Zhi-Jun Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：Applied Microbiology and Biotechnology 2012
- 关键词：Data mining - Hydrolysis - Lanthanum compounds - Enantioselectivity
- Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst被引量：31收藏
- 作者：Yu, Hui-Lei Xu, Jian-He Lu, Wen-Ya Lin, Guo-Qiang
- 机构：E China Univ Sci & Technol;Chinese Acad Sci
- 来源：JOURNAL OF BIOTECHNOLOGY 2008
- 关键词：biocatalysis apple seed meal glycoside synthesis salidroside green chemistry
- Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of dl-menthyl acetate被引量：31收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Li, Chun-Xiu Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Bacteriology - Monoterpenes - Nitrogen compounds - Cloning - Esters - Enzyme immobilization
- Significantly improved asymmetric oxidation of sulfide with resting cells of Rhodococcus sp. in a biphasic system被引量：30收藏
- 作者：Li, Ai-Tao Zhang, Jian-Dong Yu, Hui-Lei Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Process Biochemistry 2011
- 关键词：Oxidation - Enantiomers - Paraffins - Alcohols - Polyols - Solvent extraction
- A new thermostable β-glucosidase mined from Dictyoglomus thermophilum: Properties and performance in octyl glucoside synthesis at high temperatures被引量：30收藏
- 作者：Zou, Zheng-Zheng Yu, Hui-Lei Li, Chun-Xiu Zhou, Xin-Wen Hayashi, Chihiro Sun, Jie Liu, Bao-Hong Imanaka, Tadeyuki Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; Department of Chemistry and Institute of Biomedical Sciences; Department of Biotechnology
- 来源：Bioresource Technology 2012
- 关键词：Cloning - Glucose - Solutions
- Improving Pseudomonas sp. esterase performance by engineering approaches for kinetic resolution of 2-acetoxyphenylacetic acids被引量：29收藏
- 作者：Ju, Xin Pan, Jiang Yu, Hui-Lei Li, Chun-Xiu Xu, Jian-He
- 机构： Lab. of Biocatalysis and Synthetic Biotechnology
- 来源：Biochemical Engineering Journal 2011
- 关键词：Bacteria - Cells - Enzymes - Organic acids - Hydrolysis - Molecular biology - Cell culture - Esters
- One-Pot Synthesis of Phenylglyoxylic Acid from Racemic Mandelic Acids via Cascade Biocatalysis被引量：28收藏
- 作者：Tang, Cun-Duo Ding, Peng-Ju Shi, Hong-Ling Jia, Yuan-yuan Zhou, Mao-zhi Yu, Hui-lei Xu, Jian-He Yao, Lun-Guang Kan, Yun-Chao
- 机构：Nanyang Normal Univ;Nanyang Normal Univ;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：mandelate dehydrogenase mandelate racemase cascade reaction phenylglyoxylic acid whole cell catalysis
- Bioamination of alkane with ammonium by an artificially designed multienzyme cascade被引量：28收藏
- 作者：Yu, Hui-Lei Li, Tuo Chen, Fei-Fei Luo, Xiao-Jing Li, Aitao Yang, Chao Zheng, Gao-Wei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Hubei Univ;Nankai Univ;Nankai Univ
- 来源：METABOLIC ENGINEERING 2018
- 关键词：Systems biocatalysis Cascade reaction C-H amination Cyclohexylamine
- Facile synthesis of alkyl β-D-glucopyranosides from D-glucose and the corresponding alcohols using fruit seed meals被引量：27收藏
- 作者：Lu, Wen-Ya Lin, Guo-Qiang Yu, Hui-Lei Tong, Ai-Ming Xu, Jian-He
- 机构：Chinese Acad Sci;E China Univ Sci & Technol
- 来源：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 2007
- 关键词：almond meal fruit seed meal beta-glucosidase reverse hydrolysis
- Immobilization of Serratia marcescens lipase onto amino-functionalized magnetic nanoparticles for repeated use in enzymatic synthesis of Diltiazem intermediate被引量：27收藏
- 作者：Hu, Bin Pan, Jiang Yu, Hui-Lei Liu, Jian-Wen Xu, Jian-He
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Process Biochemistry 2009
- 关键词：Nanoparticles - ProtEIns - High resolution transmission electron microscopy - Reaction intermediates - Fourier transform infrared spectroscopy - Magnetite - Coprecipitation - Nanomagnetics - Synthesis (chemical)
