成果/Result
- Engineering of Cyclohexanone Monooxygenase for the Enantioselective Synthesis of (S)-Omeprazole被引量：59收藏
- 作者：Zhang, Yan Wu, Yin-Qi Xu, Na Zhao, Qian Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangsu Aosaikang Pharmaceut Co Ltd
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2019
- 关键词：Biocatalysis Cyclohexanone monooxygenase Substrate specificity ProtEIn engineering Esomeprazole sulfoxide Halo-based selection method
- Enzymatic Preparation of the Chiral (S)-Sulfoxide Drug Esomeprazole at Pilot-Scale Levels被引量：45收藏
- 作者：Xu, Na Zhu, Jun Wu, Yin-Qi Zhang, Yan Xia, Jian-Ye Zhao, Qian Lin, Guo-Qiang Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangsu Aosaikang Pharmaceut Co Ltd
- 来源：ORGANIC PROCESS RESEARCH & DEVELOPMENT 2020
- 关键词：prazole sulfide monooxygenase esomeprazole asymmetric oxidation oxygen mass transfer reaction regulation
- LuxO controls extracellular protease, haemolytic activities and siderophore production in fish pathogen Vibrio alginolyticus被引量：38收藏
- 作者：Wang, Q. Liu, Q. Ma, Y. Rui, H. Zhang, Y.
- 机构： State Key Laboratory of Bioreactor Engineering; State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Applied Microbiology 2007
- 关键词：Cloning - Fish - Physiology
- Dynamic Metabolism Mechanism of Halomonas Elongata Dsm 2581t Response to Nacl Shock Stress: An Effective Technology for Enhancing Ectoine Biosynthesis被引量：36收藏
- 作者：Yu, Junxiong Zhang, Yue Wang, Jing Liu, Yuxuan Mohisn, Ali Liu, Hao Zheng, Yanning Xing, Jianmin Zhuang, Yingping Guo, Meijin Wang, Zejian
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Chemical Engineering for Energy Resources; State Key Laboratory of Microbial Resources; College of Chemical Engineering
- 来源：SSRN 2023
- 关键词：Biochemistry - Biosynthesis - Industrial research - Industrial water treatment - Metabolism - Metabolites - Wastewater treatment
- Discovery of Two Native Baeyer-Villiger Monooxygenases for Asymmetric Synthesis of Bulky Chiral Sulfoxides被引量：33收藏
- 作者：Zhang, Yan Liu, Feng Xu, Na Wu, Yin-Qi Zheng, Yu-Cong Zhao, Qian Lin, Guoqiang Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Jiangsu Aosaikang Pharmaceut Co Ltd;Chinese Acad Sci
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2018
- 关键词：Baeyer-Villiger monooxygenase ethionamide monooxygenase stereoselectivity asymmetric sulfoxidation prazole thioether
- Determination of β-adrenergic agonists by hapten microarray被引量：32收藏
- 作者：Zuo, Peng Zhang, Yin Liu, Jie Ye, Bang-Ce
- 机构：E China Univ Sci & Technol
- 来源：TALANTA 2010
- 关键词：beta-Adrenergic agonist Residues Microarray Multiplexed determination
- Engineering Pichia pastoris to improve S-adenosyl- l-methionine production using systems metabolic strategies被引量：32收藏
- 作者：Qin, Xiulin Lu, Junjie Zhang, Yin Wu, Xiaole Qiao, Xuefeng Wang, Zhipeng Chu, Ju Qian, Jiangchao
- 机构：East China Univ Sci & Technol;Guangxi Univ
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2020
- 关键词：metabolic engineering Pichia pastoris promoter library S-adenosyl-L-methionine transcriptome profiling
- Enhancement of alkaline protease production in recombinant Bacillus licheniformis by response surface methodology被引量：31收藏
- 作者：Zhang, Ying Hu, Jingmin Zhang, Qing Cai, Dongbo Chen, Shouwen Wang, Yonghong
- 机构：East China Univ Sci & Technol;Hubei Univ
- 来源：BIORESOURCES AND BIOPROCESSING 2023
- 关键词：Alkaline protease Recombinant Bacillus licheniformis Medium optimization Response surface methodology
- Modeling and optimization of membrane preparation conditions of the alginate-based microcapsules with response surface methodology被引量：30收藏
- 作者：Ma, Ying Zhang, Ying Zhao, Shan Wang, Yu Wang, Siran Zhou, Yan Li, Na Xie, Hongguo Yu, Weiting Liu, Yang Wang, Wei Ma, Xiaojun
- 机构：Chinese Acad Sci;Chinese Acad Sci;E China Univ Sci & Technol
- 来源：JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 2012
- 关键词：microencapsulation membrane preparation optimization conditions response surface methodology modeling
- Mutation breeding of high avermectin B1a-producing strain by the combination of high energy carbon heavy ion irradiation and sodium nitrite mutagenesis based on high throughput screening被引量：24收藏
- 作者：Song, Xiaoqing Zhang, Yun Zhu, Xudong Wang, Yonghong Chu, Ju Zhuang, Yingping
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol
- 来源：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 2017
- 关键词：avermectin B-1a miniaturization culture evaluation of mutagenesis method high-throughput screening
- Efficient gene deletion and replacement in Aspergillus niger by modified in vivo CRISPR/Cas9 systems被引量：23收藏
- 作者：Zhang, Yuan Ouyang, Liming Nan, Yilin Chu, Ju
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2019
- 关键词：Aspergillus niger CRISPR/Cas9 Filamentous fungi Genome editing Ribozyme pAN7-1 Glucoamylase
- Tolerance of Trichosporon cutaneum to lignin derived phenolic aldehydes facilitate the cell growth and cellulosic lipid accumulation被引量：20收藏
- 作者：Zhang, Yi Bao, Jie
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF BIOTECHNOLOGY 2022
- 关键词：Lignocellulose Oleaginous yeast Trichosporon cutaneum Phenolic aldehyde Microbial lipid
- Partition of spiramycin in a recyclable aqueous two-phase system based on pH-responsive and thermosensitive polymers被引量：19收藏
- 作者：Zhang, Yan Zhang, Hongzhou He, Dong Cao, Xuejun Wan, Junfen
- 机构：East China Univ Sci & Technol;Henan Topfond Pharmaceut Co Ltd
- 来源：PROCESS BIOCHEMISTRY 2020
- 关键词：Aqueous two-phase system Partition Recovery Spiramycin
- Design and optimization of E. coli artificial genetic circuits for detection of explosive composition 2,4-dinitrotoluene被引量：18收藏
- 作者：Zhang, Yan Zou, Zhen-Ping Chen, Sheng-Yan Wei, Wen-Ping Zhou, Ying Ye, Bang-Ce
- 机构：East China Univ Sci & Technol;Shihezi Univ;Zhejiang Univ Technol;East China Univ Sci & Technol
- 来源：BIOSENSORS & BIOELECTRONICS 2022
- 关键词：Whole-cell biosensor Genetic circuit 2,4-Dinitrotoluene Explosives Synthetic biology
- Enhancing nemadectin production by Streptomyces cyaneogriseus ssp noncyanogenus through quantitative evaluation and optimization of dissolved oxygen and shear force被引量：18收藏
- 作者：Song, Xiaoqing Zhang, Yun Xue, Jiayun Li, Chao Wang, Zejian Wang, Yonghong
- 机构：East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2018
- 关键词：Nemadectin Oxygen supply Shear stress Impeller configuration Computational fluid dynamics
- Cloning and Characterization of a Novel Esterase from Rhodococcus sp. for Highly Enantioselective Synthesis of a Chiral Cilastatin Precursor被引量：16收藏
- 作者：Zhang, Yan Pan, Jiang Luan, Zheng-Jiao Xu, Guo-Chao Park, Sunghoon Xu, Jian-He
- 机构：E China Univ Sci & Technol;Pusan Natl Univ
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2014
- 关键词：Enantioselectivity - Enzymes - Escherichia coli - Carboxylation - Hydrolysis
- Physiological metabolic topology analysis of Halomonas elongata DSM 2581T in response to sodium chloride stress被引量：15收藏
- 作者：Yu, Junxiong Wang, Zejian Wang, Jing Mohisn, Ali Liu, Hao Zhang, Yue Zhuang, Yingping Guo, Meijin
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2022
- 关键词：ectoine Halomonas elongata DSM 2581(T) kinetic NaCl stress transcriptomic
- Comparative analysis of the roles of catalases KatB and KatG in the physiological fitness and pathogenesis of fish pathogen Edwardsiella tarda被引量：14收藏
- 作者：Xiao, J. Chen, T. Wang, Q. Zhang, Y.
- 机构：E China Univ Sci & Technol
- 来源：LETTERS IN APPLIED MICROBIOLOGY 2012
- 关键词：catalase Edwardsiella tarda katB katG virulence
- Extracellular Secretion of β-glucosidase in Ethanologenic E-coli Enhances Ethanol Fermentation of Cellobiose被引量：11收藏
- 作者：Luo, Zichen Zhang, Yao Bao, Jie
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2014
- 关键词：E. coli Ethanol fermentation beta-glucosidase Secretive expression Surfactant
- Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids被引量：8收藏
- 作者：Liu, H. Gu, D. Sheng, L. Wang, Q. Zhang, Y.
- 机构：E China Univ Sci & Technol
- 来源：LETTERS IN APPLIED MICROBIOLOGY 2012
- 关键词：Asp biofilm gateway T6SS Vibrio alginolyticus
- Sensitivity improvement of rapid Vibrio harveyi detection with an enhanced chemiluminescent-based dot blot被引量：7收藏
- 作者：Li, H. Xiao, J. Zhou, Y. Wang, Q. Zhang, Y.
- 机构：East China Univ Sci & Technol;Shanghai Engn Res Ctr Maricultured Anim Vaccines;Shanghai Collaborat Innovat Ctr Biomfg
- 来源：LETTERS IN APPLIED MICROBIOLOGY 2017
- 关键词：dot blot enhanced chemiluminescent hatcheries monoclonal antibodies rapid detection Vibrio harveyi
- NADH-dependent lactate dehydrogenase from Alcaligenes eutrophus H16 reduces 2-oxoadipate to 2-hydroxyadipate被引量：6收藏
- 作者：Zhang, Yan Ashok, Somasundar Seol, Eunhee Ainala, Satish Kumar Lee, Sun-Gu Madan, Bharat Xu, Jian-He Park, Sunghoon
- 机构：Pusan Natl Univ;E China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 2014
- 关键词：adipic acid 2-Oxoadipate 2-Oxoadipate reductase 2-Oxoglutarate Alcaligenes eutrophus H16 lactate dehydrogenase
- A genome-scale metabolic model of the effect of dissolved oxygen on 1,3-propanediol fermentation by Klebsiella pneumoniae被引量：4收藏
- 作者：Zhang, Yang Yang, Menglei Bao, Yangyang Tao, Weihua Tuo, Jinyou Liu, Boya Gan, Luxi Fu, Shuilin Gong, Heng
- 机构：East China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2023
- 关键词：1 3-Propanediol Flux balance analysis Genome-scale metabolic model Klebsiella pneumoniae Microaerobic culture
- Oxygen uptake rate (OUR) guided nitrogen control strategy for improving nemadectin production by Streptomyces cyaneogriseus ssp. noncyanogenus被引量：2收藏
- 作者：Song, Xiaoqing Zhang, Zishu Yu, Junxiong Zhang, Yun Xue, Jiayun Guo, Yuanxin Liang, Jianguang Ren, Min Ling, Qingyun Mohsin, Ali Qian, Jiangchao Wang, Zejian Wang, Yonghong
- 机构：East China Univ Sci & Technol;Changshu Inst Technol;Jiangsu Weiling Pharmaceut Co Ltd
- 来源：PROCESS BIOCHEMISTRY 2025
- 关键词：Nemadectin S. cyaneogriseus On-line parameters Real-time monitoring Fed-batch fermentation
- ChIP-seq and structural analyses delineating the regulatory mechanism of master regulator EsrB in Edwardsiella piscicida被引量：2收藏
- 作者：Zhang, Boya Zhang, Yi Liu, Jingjing Reverter, David Wang, Qiyao Choi, Sang Ho Liu, Bing Shao, Shuai
- 机构：East China Univ Sci & Technol;Hangzhou Normal Univ;Univ Autonoma Barcelona;Engn Res Ctr Maricultured Anim Vaccines;Seoul Natl Univ
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2024
- 关键词：ChIP-seq crystal structure Edwardsiella piscicida EsrB metabolism pathogenesis
- Biological Sentinel: An Efficient Engineered Bacterial System for Monitoring and Tracing Regulated Hazardous Chemicals被引量：1收藏
- 作者：Zhang, Yan Zou, Zhen-Ping Chen, Sheng-Yan Zhou, Ying Ye, Bang-Ce
- 机构：East China Univ Sci & Technol;Shihezi Univ
- 来源：ANALYTICAL CHEMISTRY 2023
- 关键词：DNA sequences - Hazards
- Effective extraction of tylosin and spiramycin from fermentation broth using thermo-responsive ethylene oxide/propylene oxide aqueous two-phase systems被引量：1收藏
- 作者：Yang, Ting Zheng, Ting Wang, Yan Zhang, Yan He, Dong Zeng, Hainan Wei, Yanli Chen, Xi Wan, Junfen Cao, Xuejun
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;Henan Topfond Pharmaceut Co Ltd
- 来源：JOURNAL OF SEPARATION SCIENCE 2022
- 关键词：antibiotics ethylene oxide propylene oxide copolymer molecular dynamic simulation partition coefficient recyclable aqueous two-phase systems
- Elucidating metabolic mechanisms underlying the influence of specific growth rate on alkaline protease synthesis in Bacillus licheniformis through combined omics and computational modeling analysis被引量：1收藏
- 作者：Zhang, Ying Liu, Zhihao Hu, Jingmin Yao, Qiaoer Xu, Hongfei Zhang, Qing Chen, Shouwen Wang, Yonghong
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Hubei Univ
- 来源：BIORESOURCE TECHNOLOGY 2025
- 关键词：Alkaline protease Bacillus licheniformis Transcriptomic Genome-scale metabolic model Flux balance analysis
- 细胞生长和非生长相结合的拟干酪乳杆菌高效生产L-乳酸研究被引量：1收藏
- 作者：张悦 田锡炜 庄英萍
- 机构：华东理工大学生物工程学院;华东理工大学发酵工程实验教学示范中心;华东理工大学生物反应器工程国家重点实验室
- 来源：《食品工业科技》 2021
- 关键词：拟干酪乳杆菌 乳酸 细胞生长和非生长相结合 静息细胞 高密度发酵
- 摘要：本研究以拟干酪乳杆菌发酵生产L-乳酸为研究对象,通过对最适初始葡萄糖浓度以及稳定期静息细胞的代谢能力进行研究,开发了基于细胞生长和非生长相关组合的高细胞密度L-乳酸生产策略。研究结果表明,140 g/L初始葡萄糖浓度下,...
- 下载全文在线阅读
- Resource utilization of penicillin fermentation fungi residue for ectoine production by Halomonas elongata被引量：0收藏
- 作者：Liu, Yuxuan Zhang, Sinan Zhang, Guohui Zhang, Yue Yang, Chen Wang, Zejian Yu, Junxiong Wu, Shiyong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIORESOURCE TECHNOLOGY 2025
- 关键词：PFFR Hydrolysate Ectoine Halomonas elongata Resource utilization
