成果/Result
- Reshaping the Active Pocket of Amine Dehydrogenases for Asymmetric Synthesis of Bulky Aliphatic Amines被引量：102收藏
- 作者：Chen, Fei-Fei Zheng, Gao-Wei Liu, Lei Li, Hao Chen, Qi Li, Fu-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2018
- 关键词：amine dehydrogenase reductive amination chiral amine protEIn engineering biocatalysis substrate scope
- Preparation of Structurally Diverse Chiral Alcohols by Engineering Ketoreductase CgKR1被引量：82收藏
- 作者：Zheng, Gao-Wei Liu, Yuan-Yang Chen, Qi Huang, Lei Yu, Hui-Lei Lou, Wen-Yong Li, Chun-Xiu Bai, Yun-Peng Li, Ai-Tao Xu, Jian-He
- 机构：East China Univ Sci & Technol;South China Univ Technol;Max Planck Inst Kohlenforsch
- 来源：ACS CATALYSIS 2017
- 关键词：asymmetric reduction ketoreductase chiral alcohol protEIn engineering substrate specificity biocatalysis
- Biocatalytic properties of a recombinant aldo-keto reductase with broad substrate spectrum and excellent stereoselectivity被引量：68收藏
- 作者：Ni, Yan Li, Chun-Xiu Ma, Hong-Min Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：YtbE Recombinant aldo-keto reductase Bacillus sp. Asymmetric reduction Chiral alcohols
- Two-step enzymatic synthesis of ursodeoxycholic acid with a new 7β-hydroxysteroid dehydrogenase from Ruminococcus torques被引量：66收藏
- 作者：Zheng, Ming-Min Wang, Ru-Feng Li, Chun-Xiu Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;China Pharmaceut Univ
- 来源：PROCESS BIOCHEMISTRY 2015
- 关键词：Ursodeoxycholic acid Chenodeoxycholic acid 7 alpha-Hydroxysteroid dehydrogenase 7 beta-Hydroxysteroid dehydrogenase Two-step reaction
- Switching Cofactor Dependence of 7β-Hydroxysteroid Dehydrogenase for Cost-Effective Production of Ursodeoxycholic Acid被引量：66收藏
- 作者：You, Zhi-Neng Chen, Qi Shi, Shou-Cheng Zheng, Ming-Min Pan, Jiang Qian, Xiao-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：ACS CATALYSIS 2019
- 关键词：biocatalysis cofactor dependence small-and-smart library 7 beta-hydroxysteroid dehydrogenase molecular dynamics simulation ursodeoxycholic acid
- Engineering 7β-Hydroxysteroid Dehydrogenase for Enhanced Ursodeoxycholic Acid Production by Multiobjective Directed Evolution被引量：57收藏
- 作者：Zheng, Ming-Min Chen, Ke-Cai Wang, Ru-Feng Li, Hao Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：biocatalysis cascade reaction 7 beta-hydroxysteroid dehydrogenase multiobjective directed evolution ursodeoxycholic acid
- Highly stereoselective reduction of prochiral ketones by a bacterial reductase coupled with cofactor regeneration被引量：46收藏
- 作者：Ni, Yan Li, Chun-Xiu Wang, Li-Juan Zhang, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC & BIOMOLECULAR CHEMISTRY 2011
- 关键词：Aromatic compounds - Bacteriology - Enantiomers - Ketones
- Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. Coli被引量：38收藏
- 作者：Du, Fu-Liang Yu, Hui-Lei Xu, Jian-He Li, Chun-Xiu
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Biochemistry - Biosynthesis - Flavor compounds - Gene expression - Monoterpenes
- Enzymatic synthesis of high-titer nicotinamide mononucleotide with a new nicotinamide riboside kinase and an efficient ATP regeneration system被引量：34收藏
- 作者：Qian, Xiao-Long Dai, Yi-Si Li, Chun-Xiu Pan, Jiang Xu, Jian-He Mu, Bozhong
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd
- 来源：BIORESOURCES AND BIOPROCESSING 2022
- 关键词：Enzymatic phosphorylation Nicotinamide riboside Nicotinamide riboside kinase beta-Nicotinamide mononucleotide (NMN) ATP regeneration system
- Highly efficient synthesis of chiral alcohols with a novel NADH-dependent reductase from Streptomyces coelicolor被引量：34收藏
- 作者：Wang, Li-Juan Li, Chun-Xiu Ni, Yan Zhang, Jie Liu, Xiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; School of Chemical and Material Engineering
- 来源：Bioresource Technology 2011
- 关键词：Enzyme activity - Ketones - Escherichia coli
- Identification of a new thermostable and alkali-tolerant α-carbonic anhydrase from Lactobacillus delbrueckii as a biocatalyst for CO2 biomineralization被引量：33收藏
- 作者：Li, Chun-Xiu Jiang, Xiao-Chen Qiu, Yong-Juan Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; Shanghai Collaborative Innovation Center for Biomanufacturing
- 来源：Bioresources and Bioprocessing 2015
- 关键词：Affinity chromatography - Alkalinity - Biomineralization - Calcite - Calcium carbonate - Dimers - Escherichia coli - Stability
- Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata被引量：32收藏
- 作者：Zhang, Chen-Sheng Zhang, Zhi-Jun Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：Applied Microbiology and Biotechnology 2012
- 关键词：Data mining - Hydrolysis - Lanthanum compounds - Enantioselectivity
- Crosslinking of enzyme coaggregate with polyethylenEImine: A simple and promising method for preparing stable biocatalyst of Serratia marcescens lipase被引量：31收藏
- 作者：Pan, Jiang Kong, Xu-Dong Li, Chun-Xiu Ye, Qin Xu, Jian-He Imanaka, Tadayuki
- 机构： State Key Laboratory of Bioreactor Engineering; Department of Biotechnology
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Coprecipitation - Enzyme immobilization
- Immobilization of Bacillus subtilis esterase by simple cross-linking for enzymatic resolution of dl-menthyl acetate被引量：31收藏
- 作者：Zheng, Gao-Wei Yu, Hui-Lei Li, Chun-Xiu Pan, Jiang Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Bacteriology - Monoterpenes - Nitrogen compounds - Cloning - Esters - Enzyme immobilization
- De Novo Multienzyme Synthetic Pathways for Lactic Acid Production被引量：31收藏
- 作者：Ding, Xu-Wei Rong, Jing Pan, Ze-Peng Zhu, Xin-Xin Zhu, Zhen-Yu Chen, Qi Zhang, Zhi-Jun Xu, Jian-He Li, Chun-Xiu Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2024
- 关键词：biocatalysis C1 compound lacticacid multienzyme cascade protEIn engineering
- A new thermostable β-glucosidase mined from Dictyoglomus thermophilum: Properties and performance in octyl glucoside synthesis at high temperatures被引量：30收藏
- 作者：Zou, Zheng-Zheng Yu, Hui-Lei Li, Chun-Xiu Zhou, Xin-Wen Hayashi, Chihiro Sun, Jie Liu, Bao-Hong Imanaka, Tadeyuki Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; Department of Chemistry and Institute of Biomedical Sciences; Department of Biotechnology
- 来源：Bioresource Technology 2012
- 关键词：Cloning - Glucose - Solutions
- A Novel (R)-Imine Reductase from Paenibacillus lactis for Asymmetric Reduction of 3H-Indoles被引量：29收藏
- 作者：Li, Hao Zhang, Guang-Xiang Li, Liu-Mei Ou, Yu-Shi Wang, Ming-Yang Li, Chun-Xiu Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CHEMCATCHEM 2016
- 关键词：asymmetric reduction biocatalysis imine reductase indoles indolines
- Improving Pseudomonas sp. esterase performance by engineering approaches for kinetic resolution of 2-acetoxyphenylacetic acids被引量：29收藏
- 作者：Ju, Xin Pan, Jiang Yu, Hui-Lei Li, Chun-Xiu Xu, Jian-He
- 机构： Lab. of Biocatalysis and Synthetic Biotechnology
- 来源：Biochemical Engineering Journal 2011
- 关键词：Bacteria - Cells - Enzymes - Organic acids - Hydrolysis - Molecular biology - Cell culture - Esters
- Enantioselective production of (S)-1-phenyl-1,2-ethanediol from dicarboxyesters by recombinant Bacillus subtilis esterase被引量：27收藏
- 作者：Tian, Xin Zheng, Gao-Wei Li, Chun-Xiu Wang, Zhi-Long Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology; School of Pharmacy
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Chlorine compounds - Enantioselectivity - Liquid crystal displays - Enzymes - Esters - Bacteriology - Hydrolysis
- Remodeling the Homologous Recombination Mechanism of Yarrowia lipolytica for High-Level Biosynthesis of Squalene被引量：26收藏
- 作者：Xu, Man Yang, Nan Pan, Jiang Hua, Qiang Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2024
- 关键词：homologous recombination squalene Yarrowia lipolytica metabolic engineering acetyl coenzyme A
- An efficient bioprocess for enzymatic production of l-menthol with high ratio of substrate to catalyst using whole cells of recombinant E. coli被引量：26收藏
- 作者：Zheng, Gao-Wei Pan, Jiang Yu, Hui-Lei Ngo-Thi, Minh-Thu Li, Chun-Xiu Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Bioprocessing
- 来源：Journal of Biotechnology 2010
- 关键词：Bacteriology - Cloning - Escherichia coli - Monoterpenes - Esters - Cytology - Gene encoding - Catalysis - Enzymes - Loading
- Combinatorial Evolution of Phosphotriesterase Toward a Robust Malathion Degrader by Hierarchical Iteration Mutagenesis被引量：25收藏
- 作者：Luo, Xiao-Jing Zhao, Jian Li, Chun-Xiu Bai, Yun-Peng Reetz, Manfred T. Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Max Planck Inst Kohlenforsch;Philipps Univ Marburg
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2016
- 关键词：biocatalysis directed evolution DNA shuffling malathion phosphotriesterase
- Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp nitrilase被引量：24收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2014
- 关键词：Immobilized cells Alcaligenes sp nitrilase Glutaraldehyde cross-linking Enantioselective hydrolysis (R)-(-)-Mandelic acid
- Fingerprint lipolytic enzymes with chromogenic p-nitrophenyl esters of structurally diverse carboxylic acids被引量：24收藏
- 作者：Qian, Le Liu, Jia-Yan Liu, Jia-Ying Yu, Hui-Lei Li, Chun-Xiu Xu, Jian-He
- 机构： Laboratory of Biocatalysis and Synthetic Biotechnology
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Data mining - Substrates - Lipases
- Catalytic Performance of Corn Stover Hydrolysis by a New Isolate Penicillium sp ECU0913 Producing both Cellulase and Xylanase被引量：24收藏
- 作者：Shi, Qian-Qian Sun, Jie Yu, Hui-Lei Li, Chun-Xiu Bao, Jie Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Penicillium sp. Cellulase Xylanase Enzymatic hydrolysis Corn stover
- Cloning of thermostable cellulase genes of Clostridium thermocellum and thEIr secretive expression in Bacillus subtilis被引量：24收藏
- 作者：Liu, Jian-Ming Xin, Xiu-Juan Li, Chun-Xiu Xu, Jian-He Bao, Jie
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Biochemistry and Biotechnology 2012
- 关键词：Bacteriology - Clostridium - Strain - Cellulose - Gene expression
- Enhancing transglutaminase production of Streptomyces mobaraensis by iterative mutagenesis breeding with atmospheric and room-temperature plasma (ARTP)被引量：19收藏
- 作者：Jiang, Ying Shang, Yue-Peng Li, Hao Zhang, Chao Pan, Jiang Bai, Yun-Peng Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：BIORESOURCES AND BIOPROCESSING 2017
- 关键词：Atmospheric and room-temperature plasma Breeding Streptomyces mobaraensis Transcription level Transglutaminase
- Asymmetric bioreduction of keto groups of 4-and 5-Oxodecanoic acids/esters with a new carbonyl reductase被引量：19收藏
- 作者：Zhang, Chao Pan, Jiang Li, Chun-Xiu Bai, Yun-Peng Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：CATALYSIS COMMUNICATIONS 2017
- 关键词：4-Oxodecanoic acid 5-Oxodecanoic acid (R)-gamma-Decalactone (R)-delta-Decalactone Carbonyl reductase Asymmetric reduction
- Design of a self-sufficient hydride-shuttling cascade for concurrent bioproduction of 7,12-dioxolithocholate and l-tert-leucine被引量：18收藏
- 作者：You, Zhi-Neng Zhou, Ke Han, Yu Yang, Bing-Yi Chen, Qi Pan, Jiang Qian, Xiao-Long Li, Chun-Xiu Xu, Jian-He
- 机构：East China Univ Sci & Technol;Suzhou Bioforany Enzynch Co Ltd
- 来源：GREEN CHEMISTRY 2021
- 关键词：Amines - Cost effectiveness - Hydrides
- Efficient Degradation of Malathion in the Presence of Detergents Using an Engineered Organophosphorus Hydrolase Highly. Expressed by Pichia pastoris without Methanol Induction被引量：17收藏
- 作者：Bai, Yun-Peng Luo, Xiao-Jing Zhao, Yu-Lian Li, Chun-Xiu Xu, Dian-Sheng Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2017
- 关键词：biodegradation Pichia pastoris organophosphorus hydrolase extracellular expression malathion
