成果/Result
- Biomanufacturing of Biobased Polymers被引量：337收藏
- 作者：Luo, Yuanchan Wu, Ju Fei, Peng Li, Jianing Xu, Mingcheng Guo, Pengye Zhao, Fei Qin, Zhitian Wu, Hui Fan, Liqiang Wang, Yibing Chen, Yijia Shi, Jinqi Zhao, Liming
- 机构： East China University of Science and Technology; State Key Laboratory of Bioreactor Engineering; MOE Key Laboratory of Bio-Intelligent Manufacturing
- 来源：Research and Applications of Bio-based Degradable Materials 2025
- 关键词：Biological materials - Natural resources
- Antioxidant properties of a vegetable-fruit beverage fermented with two Lactobacillus plantarum strains被引量：85收藏
- 作者：Yang, Xiaoxing Zhou, Jiachun Fan, Liqiang Qin, Zhen Chen, Qiming Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：FOOD SCIENCE AND BIOTECHNOLOGY 2018
- 关键词：Lactobacillus plantarum Vegetable-fruit beverage Fermentation Metabolites Antioxidant activities
- Progress in the biosynthesis of bio-based PET and PEF polyester monomers被引量：76收藏
- 作者：Cui, Yanan Deng, Chen Fan, Liqiang Qiu, Yongjun Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI
- 来源：GREEN CHEMISTRY 2023
- 关键词：Biochemistry - Biomass - Biosynthesis - Conservation - Cost effectiveness - Enzymes - Ethylene - Monomers - Natural resources - Petroleum industry - Plastic bottles - Polyethylene terephthalates
- Chitooligosaccharides display anti-tumor effects against human cervical cancer cells via the apoptotic and autophagic pathways被引量：57收藏
- 作者：Zhao, Mengyao Gu, Liming Li, Yun Chen, Shumin You, Jiangshan Fan, Liqiang Wang, Yudong Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ;SCICBT
- 来源：CARBOHYDRATE POLYMERS 2019
- 关键词：Chitooligosaccharides Enzyme-membrane coupling reactor Apoptosis ROS production Autophagy
- Insights into the nanofiltration separation mechanism of monosaccharides by molecular dynamics simulation被引量：49收藏
- 作者：Yao, Lei Qin, Zhen Chen, Qiming Zhao, Mengyao Zhao, Hefei Ahmad, Waheed Fan, Liqiang Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SC;Univ Nebraska
- 来源：SEPARATION AND PURIFICATION TECHNOLOGY 2018
- 关键词：Monosaccharide Nanofiltration Molecular dynamics simulation Diffusion coefficient Interaction energy
- Investigating the mechanism of nanofiltration separation of glucosamine hydrochloride and N-acetyl glucosamine被引量：44收藏
- 作者：Zhang, Shengnan Zhou, Jiachun Fan, Liqiang Qiu, Yongjun Jiang, Lihua Zhao, Liming
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai Collaborative Innovation Center for Biomanufacturing Technology
- 来源：Bioresources and Bioprocessing 2016
- 关键词：Bioactivity - Chemical reactions - Chitin - Efficiency - Electrolytes - Glucosamine - Magnesium compounds - Sodium chloride - Temperature
- High level production of a Bacillus amlyoliquefaciens chitosanase in Pichia pastoris suitable for chitooligosaccharides preparation被引量：41收藏
- 作者：Luo, Sa Qin, Zhen Chen, Qiming Fan, Liqiang Jiang, Lihua Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2020
- 关键词：Chitosanase Chitooligosaccharides Glycoside hydmlase family 46
- Regioselectivity Engineering of Epoxide Hydrolase: Near-Perfect Enantioconvergence through a Single Site Mutation被引量：41收藏
- 作者：Li, Fu-Long Kong, Xu-Dong Chen, Qi Zheng, Yu-Cong Xu, Qin Chen, Fei-Fei Fan, Li-Qiang Lin, Guo-Qiang Zhou, Jiahai Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Chinese Acad Sci;Shanghai Jiao Tong Univ;Shanghai Jiao Tong Univ
- 来源：ACS CATALYSIS 2018
- 关键词：biocatalysis epoxide hydrolase crystal structure regioselectivity enantioconvergence protEIn engineering
- Refolding, purification and characterization of an organic solvent-tolerant lipase from Serratia marcescens ECU1010被引量：37收藏
- 作者：Li, Suxia Pang, Huaiyu Lin, Kang Xu, Jianhe Zhao, Jian Fan, Liqiang
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Journal of Molecular Catalysis B: Enzymatic 2011
- 关键词：Cloning - Chromatography - Recombinant protEIns - Purification - Organic solvents
- Expression and Characterization of a Novel Enantioselective Lipase from Aspergillus fumigatus被引量：34收藏
- 作者：Shangguan, Jiao-Jiao Fan, Li-Qiang Ju, Xin Zhu, Qing-Qing Wang, Fu-Jun Zhao, Jian Xu, Jian-He
- 机构： State Key Laboratory of Bioreactor Engineering; Institute of Chinese Materia Medica
- 来源：Applied Biochemistry and Biotechnology 2012
- 关键词：Aspergillus - Cloning - Fatty acids - Purification - Escherichia coli - Enantioselectivity
- Efficient Immobilization of Bacterial GH Family 46 Chitosanase by Carbohydrate-Binding Module Fusion for the Controllable Preparation of Chitooligosaccharides被引量：33收藏
- 作者：Lin, Si Qin, Zhen Chen, Qiming Fan, Liqiang Zhou, Jiachun Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 2019
- 关键词：chitosanase carbohydrate binding module chitooligosaccharide one-step immobilization-purification packed-bed reactor
- The involvement of oxidative stress, neuronal lesions, neurotransmission impairment, and neuroinflammation in acrylamide-induced neurotoxicity in C57/BL6 mice被引量：33收藏
- 作者：Zhao, Mengyao Deng, Linlin Lu, Xiaoxuan Fan, Liqiang Zhu, Yang Zhao, Liming
- 机构：East China Univ Sci & Technol;Wageningen Univ & Res
- 来源：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH 2022
- 关键词：acrylamide Oxidative stress Neurotoxicity Neurotransmission impairment Neuroinflammation NLRP3 inflammasome
- Significantly improved expression and biochemical properties of recombinant serratia marcescens lipase as robust biocatalyst for kinetic resolution of chiral ester被引量：33收藏
- 作者：Wang, Yi Zhao, Jian Xu, Jian-He Fan, Li-Qiang Li, Su-Xia Zhao, Li-Li Mao, Xiao-Bo
- 机构： State Key Laboratory of Bioreactor Engineering
- 来源：Applied Biochemistry and Biotechnology 2010
- 关键词：Esters - Gene expression - Recombinant protEIns - Escherichia coli - Fatty acids - Affinity chromatography - Lipases - Substrates - Cloning - Metal ions - Metals - Nonionic surfactants - Purification
- Expression and Characterization of a Novel Lipase from Aspergillus fumigatus with High Specific activity被引量：31收藏
- 作者：Shangguan, Jiao-Jiao Liu, Yu-Qiang Wang, Fu-Jun Zhao, Jian Fan, Li-Qiang Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol;Shanghai Univ Chinese Tradit Med
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2011
- 关键词：Aspergillus fumigatus Recombinant lipase Expression Characterization active site
- Increasing thermal stability of glutamate decarboxylase from Escherichia. coli by site-directed saturation mutagenesis and its application in GABA production被引量：30收藏
- 作者：Fan, Li-Qiang Li, Ming-Wei Qiu, Yong-jun Chen, Qi-ming Jiang, Si-Jing Shang, Yu-Jie Zhao, Li-Ming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：JOURNAL OF BIOTECHNOLOGY 2018
- 关键词：Glutamate decarboxylase gamma-Aminobutyrate Site-directed saturation mutagenesis Thermostability Hydrogen bonds
- Heterologous overexpression of Vigna radiata epoxide hydrolase in Escherichia coli and its catalytic performance in enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol被引量：29收藏
- 作者：Zhu, Qing-Qing He, Wan-Hong Kong, Xu-Dong Fan, Li-Qiang Zhao, Jian Li, Su-Xia Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2014
- 关键词：RT-PCR Vigna radiata epoxide hydrolase p-Nitrostyrene oxide Enantioconvergency Characterization
- Bioconversion of chitin into chitin oligosaccharides using a novel chitinase with high chitin-binding capacity被引量：26收藏
- 作者：Zhao, Qiong Fan, Liqiang Deng, Chen Ma, Chunyu Zhang, Chunyue Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI;East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2023
- 关键词：Chitinase Chitin oligosaccharides Purification Molecular simulation Biodegradation
- Purification of DP 6 to 8 chitooligosaccharides by nanofiltration from the prepared chitooligosaccharides syrup被引量：25收藏
- 作者：Dong, Huizhong Wang, Yaosong Zhao, Liming Zhou, Jiachun Xia, Quanming Jiang, Lihua Fan, Liqiang
- 机构： State Key Laboratory of Bioreactor Engineering; Shanghai Collaborative Innovation Center for Biomanufacturing Technology
- 来源：Bioresources and Bioprocessing 2014
- 关键词：Desorption - Hydrogen bonds - Mass spectrometry - Polymerization - Purification
- Improvement of Ethanol Productivity and Energy Efficiency by Degradation of Inhibitors Using Recombinant Zymomonas mobilis (pHW20a-fdh)被引量：21收藏
- 作者：Dong, Hong-Wei Fan, Li-Qiang Luo, Zichen Zhong, Jian-Jiang Ryu, Dewey D. Y. Bao, Jie
- 机构：E China Univ Sci & Technol;Shanghai Res Inst Chem Ind;Shanghai Jiao Tong Univ;Univ Calif Davis
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2013
- 关键词：recombinant Z mobilis ZM4 (pHW20a-fdh) formate dehydrogenase (fdh) NADH regeneration growth inhibitor inhibitor degradation corn stover hydrolysate (CSH)
- 重组人锰超氧化物歧化酶工程菌高效表达培养工艺的研究被引量：21收藏
- 作者：张艳红 廖晓全 范立强 袁勤生
- 机构：华东理工大学生物工程学院国家重点实验室
- 来源：《中国药学杂志》 2002
- 关键词：重组人锰超氧化物歧化酶 工程菌 培养工艺 rhMn-SOD
- 摘要：目的 确定重组人锰超氧化物歧化酶 (rhMn SOD)工程菌高效表达的最适培养工艺条件。方法 通过摇瓶培养研究了包括接种量、诱导时机、Mn2 + 浓度、诱导后培养时间、发酵过程中 pH变化以及初始 pH值对发酵的影响。 ...
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- Chemoenzymatic enantioconvergent hydrolysis of p-nitrostyrene oxide into (R)-p-nitrophenyl glycol by a newly cloned epoxide hydrolase VrEH2 from Vigna radiata被引量：21收藏
- 作者：Wu, Yan-Wen Kong, Xu-Dong Zhu, Qing-Qing Fan, Li-Qiang Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：CATALYSIS COMMUNICATIONS 2015
- 关键词：Vigna radiata epoxide hydrolase Recombinant expression Enzymatic property Enantioconvergency Chemoenzymatic synthesis (R)-p-nitrophenyl glycol
- De novo biosynthesis of 2′-fucosyllactose in engineered Pichia pastoris被引量：18收藏
- 作者：Qian, Difan Zhang, Chunyue Deng, Chen Zhou, Mian Fan, Liqiang Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Changzheng Hosp;Shanghai Collaborat Innovat Ctr Biomfg Technol SCI
- 来源：BIOTECHNOLOGY LETTERS 2023
- 关键词：Biosynthesis 2 '-Fucosyllactose Human milk oligosaccharides Pichia pastoris
- Structure-function analysis of Gynuella sunshinyii chitosanase uncovers the mechanism of substrate binding in GH family 46 members被引量：18收藏
- 作者：Wang, Yani Qin, Zhen Fan, Liqiang Zhao, Liming
- 机构：East China Univ Sci & Technol;Shanghai Univ;Shanghai Collaborat Innovat Ctr Biomfg Technol SC
- 来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 2020
- 关键词：Chitooligosaccharides Chitosanase Complex structure Substrate recognition GH family 46
- An individually coated near-infrared fluorescent protEIn as a safe and robust nanoprobe for in vivo imaging被引量：16收藏
- 作者：Yang, Yu Xiang, Kun Yang, Yi-Xin Wang, Yan-Wen Zhang, Xin Cui, Yangdong Wang, Haifang Zhu, Qing-Qing Fan, Liqiang Liu, Yuanfang Cao, Aoneng
- 机构：Shanghai Univ;E China Univ Sci & Technol;Peking Univ
- 来源：NANOSCALE 2013
- 关键词：Quantum yield - Probes - Fluorescence imaging - ProtEIns - Infrared devices - Silica
- Establishment of a nanofiltration rejection sequence and calculated rejections of available monosaccharides被引量：16收藏
- 作者：Liu, Hetian Zhao, Liming Fan, Liqiang Jiang, Lihua Qiu, Yongjun Xia, Quanming Zhou, Jiachun
- 机构：E China Univ Sci & Technol;Shanghai Collaborat Innovat Ctr Biomfg Technol SC
- 来源：SEPARATION AND PURIFICATION TECHNOLOGY 2016
- 关键词：Nanofiltration Monosaccharide Molecular shape Spectrum of monosaccharide rejection
- Enhanced Low Molecular WEIght Poly-γ-Glutamic acid Production in Recombinant Bacillus subtilis 1A751 with Zinc Ion被引量：15收藏
- 作者：Jiang, Sijing Fan, Liqiang Zhao, Mengyao Qiu, Yongjun Zhao, Liming
- 机构：East China Univ Sci & Technol;SCICBT
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2019
- 关键词：Recombinant Bacillus subtilis Low molecular wEIght Poly-gamma-glutamic acid (gamma-PGA) Poly-gamma-glutamic acid synthase pgsBCAE center dot Zn2+
- Efficient inhibition of Cronobacter biofilms by chitooligosaccharides of specific molecular wEIght被引量：14收藏
- 作者：Lu, Jun Chen, Qiming Pan, Bolin Qin, Zhen Fan, Liqiang Xia, Quanming Zhao, Liming
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 2019
- 关键词：Cronobacter spp Biofilms Chitooligosaccharides
- 人铜锌超氧化物歧化酶cDNA的克隆、表达、发酵及纯化工艺研究被引量：12收藏
- 作者：施惠娟 范立强 陈长华 吴祥甫 袁勤生
- 机构：华东理工大学生物反应器工程国家重点实验室生物化学研究所;中国科学院上海生物化学研究所
- 来源：《中国药学杂志》 1999
- 关键词：铜 锌 超氧化物歧化酶 CDNA 基因克隆
- 摘要：目的：用基因工程的方法获得人铜锌超氧化物歧化酶（ｈＣｕ，Ｚｎ－ＳＯＤ）的高产菌，通过发酵培养，最后纯化获得大量廉价的高纯度的ｈＣｕ，Ｚｎ－ＳＯＤ。方法：用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ），扩增了人铜锌超氧化物歧化酶（...
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- Biocatalytic Properties of a Recombinant Fusarium proliferatum Lactonase with Significantly Enhanced Production by Optimal Expression in Escherichia coli被引量：12收藏
- 作者：Chen, Bing Fan, Li-Qiang Xu, Jian-He Zhao, Jian Zhang, Xian Ouyang, Li-Ming
- 机构：E China Univ Chem Technol
- 来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 2010
- 关键词：Levo-lactonase DL-pantoyl lactone Recombinant protEIn Immobilized metal affinity chromatography Biocatalytic property Substrate specificity
- 钝顶螺旋藻Fe-SOD的纯化及性质被引量：12收藏
- 作者：夏文超 李素霞 范立强 袁勤生
- 机构：华东理工大学生物反应器工程国家重点实验室生物工程学院
- 来源：《华东理工大学学报（自然科学版）》 2003
- 关键词：Fe-SOD 钝顶螺旋藻 铁型超氧化物歧化酶 纯化 平均比活 稳定性 金属酶
- 摘要：从螺旋藻中分离纯化的Fe-SOD平均比活为4576U/mg,总活力回收率50%,纯度达电泳纯。SDS-PAGE测定纯化的Fe-SOD亚基相对分子质量为21ku,每分子亚基含0.7个Fe原子;在Tris-盐酸缓冲液中的最适...
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