成果/Result
- Significant enhancement of (R)-mandelic acid production by relieving substrate inhibition of recombinant nitrilase in toluene-water biphasic system被引量：60收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Liu, Jun-Feng Xu, Jian-He He, Yu-Cai Liu, You-Yan
- 机构：E China Univ Sci & Technol;Changzhou Univ;Guangxi Univ
- 来源：JOURNAL OF BIOTECHNOLOGY 2011
- 关键词：Aqueous-organic biphasic system Recombinant nitrilase Enantioselective hydrolysis High substrate tolerance (R)-(-)-Mandelic acid Escherichia coli whole cells
- Efficient production of (R)-(-)-mandelic acid with highly substrate/product tolerant and enantioselective nitrilase of recombinant Alcaligenes sp.被引量：60收藏
- 作者：Zhang, Zhi-Jun Xu, Jian-He He, Yu-Cai Ouyang, Li-Ming Liu, You-Yan Imanaka, Tadayuki
- 机构：E China Univ Sci & Technol;Guangxi Univ;Ritsumeikan Univ
- 来源：PROCESS BIOCHEMISTRY 2010
- 关键词：Alcaligenes sp. Nitrilase Enantioselective Highly substrate/product tolerable (R)-(-)-Mandelic acid Fed-batch reaction
- Highly Efficient Chemoenzymatic Cascade Catalysis of Biomass into Furfurylamine by a Heterogeneous Shrimp Shell-Based Chemocatalyst and an ω-Transaminase Biocatalyst in Deep Eutectic Solvent-Water被引量：58收藏
- 作者：Ni, Jiacheng Li, Qing Gong, Lei Liao, Xiao-Long Zhang, Zhi-Jun Ma, Cuiluan He, Yucai
- 机构：Changzhou Univ;Hubei Univ;East China Univ Sci & Technol
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2021
- 关键词：furfural furfurylamine deep eutectic solvent shrimp shell corncob
- Cloning and biochemical properties of a highly thermostable and enantioselective nitrilase from Alcaligenes sp ECU0401 and its potential for (R)-(-)-mandelic acid production被引量：56收藏
- 作者：Zhang, Zhi-Jun Xu, Jian-He He, Yu-Cai Ouyang, Li-Ming Liu, You-Yan
- 机构：E China Univ Sci & Technol;Changzhou Univ;Guangxi Univ
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2011
- 关键词：Alcaligenes sp. Nitrilase Thermostable Enantioselective Biochemical properties (R)-(-)-mandelic acid
- Efficient production of (R)-o-chloromandelic acid by deracemization of o-chloromandelonitrile with a new nitrilase mined from Labrenzia aggregata被引量：53收藏
- 作者：Zhang, Chen-Sheng Zhang, Zhi-Jun Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2012
- 关键词：Nitrilase Labrenzia aggregate Enantioselective hydrolysis (R)-o-Chloromandelic acid Biphasic system
- Development of an engineered thermostable amine dehydrogenase for the synthesis of structurally diverse chiral amines被引量：44收藏
- 作者：Liu, Lei Wang, Dong-Hao Chen, Fei-Fei Zhang, Zhi-Jun Chen, Qi Xu, Jian-He Wang, Zhi-Long Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Shanghai Jiao Tong Univ
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2020
- 关键词：Ammonia - Amino acids - Amines
- Engineering Isopropanol Dehydrogenase for Efficient Regeneration of Nicotinamide Cofactors被引量：41收藏
- 作者：Jia, Qiao Zheng, Yu-Cong Li, Hai-Peng Qian, Xiao-Long Zhang, Zhi-Jun Xu, Jian-He
- 机构：East China Univ Sci & Technol;Suzhou Bioforany EnzyTech Co Ltd;East China Univ Sci & Technol
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2022
- 关键词：isopropanol dehydrogenase cofactor regeneration thermostability evolution cofactor specificity reversal protein engineering
- Increased Catalyst Productivity in α-Hydroxy Acids Resolution by Esterase Mutation and Substrate Modification被引量：39收藏
- 作者：Ma, Bao-Di Kong, Xu-Dong Yu, Hui-Lei Zhang, Zhi-Jun Dou, Shuai Xu, Yan-Peng Ni, Yan Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ACS CATALYSIS 2014
- 关键词：catalyst productivity deacylation enzymatic resolution alpha-hydroxy acid product:catalyst ratio semirational design
- Complete Genome Sequence of Industrial Biocontrol Strain Paenibacillus polymyxa HY96-2 and Further Analysis of Its Biocontrol Mechanism被引量：37收藏
- 作者：Luo, Yuanchan Cheng, Yuejuan Yi, Jincui Zhang, Zhijun Luo, Qian Zhang, Daojing Li, Yuanguang
- 机构：East China Univ Sci & Technol
- 来源：FRONTIERS IN MICROBIOLOGY 2018
- 关键词：genome sequencing Paenibacillus polymyxa comparative genomic analysis biocontrol mechanism biofilm antibiotics induced resistance
- Engineering Streptomyces coelicolor Carbonyl Reductase for Efficient Atorvastatin Precursor Synthesis被引量：36收藏
- 作者：Li, Min Zhang, Zhi-Jun Kong, Xu-Dong Yu, Hui-Lei Zhou, Jiahai Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol;Chinese Acad Sci
- 来源：APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2017
- 关键词：carbonyl reductase protein engineering catalytic activity thermostability ethyl-(S)-4-chloro-3-hydroxybutyrate
- De Novo Multienzyme Synthetic Pathways for Lactic Acid Production被引量：31收藏
- 作者：Ding, Xu-Wei Rong, Jing Pan, Ze-Peng Zhu, Xin-Xin Zhu, Zhen-Yu Chen, Qi Zhang, Zhi-Jun Xu, Jian-He Li, Chun-Xiu Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2024
- 关键词：biocatalysis C1 compound lacticacid multienzyme cascade protein engineering
- Efficient synthesis of a statin precursor in high space-time yield by a new aldehyde-tolerant aldolase identified from Lactobacillus brevis被引量：30收藏
- 作者：Jiao, Xue-Cheng Pan, Jiang Xu, Guo-Chao Kong, Xu-Dong Chen, Qi Zhang, Zhi-Jun Xu, Jian-He
- 机构：E China Univ Sci & Technol; E China Univ Sci & Technol
- 来源：CATALYSIS SCIENCE & TECHNOLOGY 2015
- 关键词：Crystal structure - Stereochemistry - Acetaldehyde - Hydrogen bonds - Ketones - Stability - Substrates
- Biocatalytic synthesis of (R)-(-)-mandelic acid from racemic mandelonitrile by cetyltrimethylammonium bromide-permeabilized cells of Alcaligenes faecalis ECU0401被引量：28收藏
- 作者：He, Yu-Cai Zhang, Zhi-Jun Xu, Jian-He Liu, You-Yan
- 机构：E China Univ Sci & Technol;Jiangsu Polytech Univ;Guangxi Univ
- 来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 2010
- 关键词：Nitrilase Alcaligenes faecalis ECU0401 Mandelonitrile (R)-(-)-Mandelic acid CTAB permeability
- An Ammonium-Formate-Driven Trienzymatic Cascade for ω-Transaminase-Catalyzed (R)-Selective Amination被引量：25收藏
- 作者：Chen, Fei-Fei Zhang, Yu-Hui Zhang, Zhi-Jun Liu, Lei Wu, Jian-Ping Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol;Zhejiang Univ
- 来源：JOURNAL OF ORGANIC CHEMISTRY 2019
- 关键词：Amino acids - Amines - Amination
- Efficient production of (R)-(-)-mandelic acid using glutaraldehyde cross-linked Escherichia coli cells expressing Alcaligenes sp nitrilase被引量：24收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Li, Chun-Xiu Yu, Hui-Lei Zheng, Gao-Wei Ju, Xin Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：BIOPROCESS AND BIOSYSTEMS ENGINEERING 2014
- 关键词：Immobilized cells Alcaligenes sp nitrilase Glutaraldehyde cross-linking Enantioselective hydrolysis (R)-(-)-Mandelic acid
- One Pot Asymmetric Synthesis of (R)-Phenylglycinol from Racemic Styrene Oxide via Cascade Biocatalysis被引量：23收藏
- 作者：Sun, Zai-Bao Zhang, Zhi-Jun Li, Fu-Long Nie, Yao Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol;Jiangnan Univ
- 来源：CHEMCATCHEM 2019
- 关键词：biocatalysis asymmetric synthesis cascade reaction in situ product removal (R)-phenylglycinol
- Identification two key residues at the intersection of domains of a thioether monooxygenase for improving its sulfoxidation performance被引量：22收藏
- 作者：Ren, Shi-Miao Liu, Feng Wu, Yin-Qi Chen, Qi Zhang, Zhi-jun Yu, Hui-Lei Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：BIOTECHNOLOGY AND BIOENGINEERING 2021
- 关键词：biocatalysis protein engineering (S)‐ omeprazole thioether monooxygenases
- Stereocomplementary Bioreduction of β-Ketonitrile without Ethylated Byproduct被引量：22收藏
- 作者：Xu, Guo-Chao Yu, Hui-Lei Zhang, Zhi-Jun Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：ORGANIC LETTERS 2013
- Efficient production of (R)-(-)-mandelic acid by isopropanol-permeabilized recombinant E. coli cells expressing Alcaligenes sp nitrilase被引量：21收藏
- 作者：Zhang, Zhi-Jun Yu, Hui-Lei Imanaka, Tadayuki Xu, Jian-He
- 机构：E China Univ Sci & Technol;E China Univ Sci & Technol;Ritsumeikan Univ
- 来源：BIOCHEMICAL ENGINEERING JOURNAL 2015
- 关键词：Biocatalysis Alcaligenes sp nitrilase Mass transfer Cell permeabilization Immobilised cells Bioprocess design
- Significantly enhanced production of recombinant nitrilase by optimization of culture conditions and glycerol feeding被引量：21收藏
- 作者：Liu, Jun-Feng Zhang, Zhi-Jun Li, Ai-Tao Pan, Jiang Xu, Jian-He
- 机构：E China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2011
- 关键词：Nitrilase Optimization Fermentation Glycerol feeding Recombinant Escherichia coli
- Efficient Transformation of Linoleic Acid into 13(S)-Hydroxy-9,11-(Z,E)-octadecadienoic Acid Using Putative Lipoxygenases from Cyanobacteria被引量：20收藏
- 作者：Qi, Yi-Ke Zheng, Yu-Cong Zhang, Zhi-Jun Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2020
- 关键词：Linoleic acid Biotransformation 13S-Hydroxyoctadecadienoic acid Lipoxygenase Cyanobacteria
- Evolution of a Catalytic Mechanism被引量：19收藏
- 作者：Rauwerdink, Alissa Lunzer, Mark Devamani, Titu Jones, Bryan Mooney, Joanna Zhang, Zhi-Jun Xu, Jian-He Kazlauskas, Romas J. Dean, Antony M.
- 机构：Univ Minnesota;Univ Minnesota;E China Univ Sci & Technol;Univ Minnesota;Sun Yat Sen Univ
- 来源：MOLECULAR BIOLOGY AND EVOLUTION 2016
- 关键词：catalysis transition state stabilization gene duplication and divergence escape from adaptive conflict innovation amplification divergence
- Effective pretreatment of sugarcane bagasse with combination pretreatment and its hydrolyzates as reaction media for the biosynthesis of ethyl (S)-4-chloro-3-hydroxybutanoate by whole cells of E-coli CCZU-K14被引量：16收藏
- 作者：He, Yu-Cai Zhang, Dan-Ping Di, Jun-Hua Wu, Yin-Qi Tao, Zhi-Cheng Liu, Feng Zhang, Zhi-Jun Chong, Gang-Gang Ding, Yun Ma, Cui-Luan
- 机构：Changzhou Univ;E China Univ Sci & Technol;Washington State Univ
- 来源：BIORESOURCE TECHNOLOGY 2016
- 关键词：Sugarcane bagasse Combination pretreatment Ethyl 4-chloro-3-oxobutanoate Ethyl (S)-4-chloro-3-hydroxybutanoate Asymmetric bioreduction
- Coevolution of the Activity and Thermostability of an ε-Keto Ester Reductase for Better Synthesis of an (R)-α-Lipoic Acid Precursor被引量：15收藏
- 作者：Xu, Yao Chen, Qi Zhang, Zhi-Jun Xu, Jian-He Zheng, Gao-Wei
- 机构：East China Univ Sci & Technol
- 来源：CHEMBIOCHEM 2020
- 关键词：asymmetric synthesis directed evolution enzyme catalysis keto ester reductases lipoic acid
- Improving the Oxygenation Performance of a Cyanobacterial Lipoxygenase by Oxygen Channel Engineering被引量：14收藏
- 作者：Qi, Yi-Ke Zheng, Yu-Cong Chen, Qi He, Yucai Zhang, Zhi-Jun Xu, Jian-He
- 机构：East China Univ Sci & Technol;Hubei Univ
- 来源：ACS SUSTAINABLE CHEMISTRY & ENGINEERING 2021
- 关键词：Biocatalysis Lipoxygenase Oxygen channel Enzyme engineering Hydroxy fatty acids
- Regio- and stereo-selective amination of fatty acids to d-amino acids by a three-step one-pot cascade被引量：13收藏
- 作者：Yu, Xing Chen, Xin-Yi Yu, Hui-Lei Xu, Jian-He Zhang, Zhi-Jun
- 机构：East China Univ Sci & Technol
- 来源：GREEN CHEMISTRY 2023
- 关键词：Amination - Amines - Amino acids - Recycling - Stereochemistry
- Computation-driven redesign of an NRPS-like carboxylic acid reductase improves activity and selectivity被引量：13收藏
- 作者：Shi, Kun Li, Ju-Mou Wang, Mu-Qiang Zhang, Yi-Ke Zhang, Zhi-Jun Chen, Qi Hollmann, Frank Xu, Jian-He Yu, Hui-Lei
- 机构：East China Univ Sci & Technol;Delft Univ Technol
- 来源：SCIENCE ADVANCES 2024
- 关键词：Biosynthesis - Carboxylation
- Characterization of a new nitrilase from Hoeflea phototrophica DFL-43 for a two-step one-pot synthesis of (S)-β-amino acids被引量：12收藏
- 作者：Zhang, Zhi-Jun Cai, Rui-Feng Xu, Jian-He
- 机构：East China Univ Sci & Technol;East China Univ Sci & Technol
- 来源：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2018
- 关键词：Biocatalysis Nitrilase omega-Transaminase Cascade reaction (S)-beta-Phenylalanine
- Efficient Biocatalytic Synthesis of Chiral Chemicals被引量：11收藏
- 作者：Zhang, Zhi-Jun Pan, Jiang Ma, Bao-Di Xu, Jian-He
- 机构：East China Univ Sci & Technol
- 来源：BIOREACTOR ENGINEERING RESEARCH AND INDUSTRIAL APPLICATIONS I: CELL FACTORIES 2016
- 关键词：Biocatalysis Data mining Efficient biocatalytic synthesis Chiral chemicals High volumetric productivity
- Hierarchical Engineering of meso-Diaminopimelate Dehydrogenase for Efficient Synthesis of Bulky D-Amino Acids被引量：10收藏
- 作者：Wei, Yan Geng, Qiang Liu, Hai-Ping Wang, Yu-Qing Zhang, Guo-Feng Qian, Xiao-Long Yu, Hui-Lei Xu, Jian-He Zhang, Zhi-Jun
- 机构：East China Univ Sci & Technol
- 来源：ACS CATALYSIS 2024
- 关键词：meso-diaminopimelate dehydrogenase asymmetric synthesis < sc > d -amino acids proteinengineering catalytic efficiency
